LOCUS       sequence-20539-e         684 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 684)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GAL-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..684
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..145)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(153..169)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(153..169)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(184..625)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(190..213)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    508..625
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(649..669)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(649..667)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ggggtgccta atgcggccgc catagtgact ggatatgttg tgttttacag tattatgtag
       61 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      121 gttcagcttt tttgtacaaa cttgtgatgg gggatccact agttctagaa tccggggttt
      181 tttctccttg acgttaaagt atagaggtat attaacaatt ttttgttgat acttttatta
      241 catttgaata agaagtaata caaaccgaaa atgttgaaag tattagttaa agtggttatg
      301 cagtttttgc atttatatat ctgttaatag atcaaaaatc atcgcttcgc tgattaatta
      361 ccccagaaat aaggctaaaa aactaatcgc attatcatcc tatggttgtt aatttgattc
      421 gttcatttga aggtttgtgg ggccaggtta ctgccaattt ttcctcttca taaccataaa
      481 agctagtatt gtagaatctt tattgttcgg agcagtgcgg cgcgaggcac atctgcgttt
      541 caggaacgcg accggtgaag acgaggacgc acggaggaga gtcttccttc ggagggctgt
      601 cacccgctcg gcggcttcta atccgtacta gagctccagc ttttgttccc tttagtgagg
      661 gttaattgcg cgcttggcgt aatc
//
