LOCUS       sequence-205409-       11240 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  stable lentiviral expression of cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11240)
  AUTHORS   Liang JR, Lingeman E, Ahmed S, Corn JE
  TITLE     Atlastins remodel the endoplasmic reticulum for selective autophagy.
  JOURNAL   J Cell Biol. 2018 Aug 24. pii: jcb.201804185. doi: 
            10.1083/jcb.201804185.
  PUBMED    30143524
REFERENCE   2  (bases 1 to 11240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2018 Aug 24. pii: jcb.201804185. doi: 10.1083/jcb.201804185."
COMMENT     Sequence Label: pLenti-X1-Neo-GFP-FAM134B
FEATURES             Location/Qualifiers
     source          1..11240
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        26..130
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(131..199,200..991)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(349..368)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1162..1750
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1651..1670
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1904..1921
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2038..2059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2074..2079,2080..2097,2098..2104)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2112..2128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2117..2139
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2136..2152
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2136..2152
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2171..2191
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2173..2191
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2219..2445
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2446..2626
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2673..2798
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3291..3524
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3709..3753
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4020..4136
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    4170..4194
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4325..5503
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4556..5494
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5451..5471
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     promoter        5520..5538
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(5583..5585,5586..5588,5589..6299)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5583..5585,5586..5588,5589..6299)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5628..5649)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5889..5908)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6236..6257
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7823..7847)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    7878..8466
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7931..7951)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8349..8360)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        8517..9016
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8552..8575)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8949..8968
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9030..9833
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9093..9112)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9703..9722
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             9977..10210
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10282..10403
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10319..10338)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10373..10392
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10443..10578
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        complement(10599..10617)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10627..10644)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10627..10643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10636..10658)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10785..11213
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10872..10891)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11082..11103
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
       61 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
      121 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
      181 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
      241 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
      301 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
      361 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
      421 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
      481 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
      541 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
      601 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
      661 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
      721 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
      781 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
      841 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
      901 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
      961 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     1021 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     1081 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     1141 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     1201 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     1261 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     1321 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     1381 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     1441 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     1501 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     1561 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     1621 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     1681 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     1741 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     1801 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     1861 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
     1921 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
     1981 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
     2041 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
     2101 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
     2161 cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc aagcttaatg
     2221 tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc
     2281 ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt
     2341 gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattgccg
     2401 cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc tctctggtta
     2461 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     2521 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     2581 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     2641 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     2701 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     2761 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     2821 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     2881 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     2941 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     3001 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     3061 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     3121 ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat
     3181 tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc
     3241 accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg
     3301 ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg
     3361 gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct
     3421 attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca
     3481 agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc
     3541 tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct
     3601 ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac
     3661 acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa
     3721 gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg
     3781 ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt
     3841 tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag
     3901 acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga
     3961 gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact
     4021 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
     4081 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa aattttatcg
     4141 tccagtgtgg tggaattctg cagatatcaa caagtttgta caaaaaagca ggctccacca
     4201 tgggaaccga cattgattat tgactaggct tttgcaaaaa gctttgcaaa gatggataaa
     4261 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
     4321 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     4381 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     4441 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     4501 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     4561 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     4621 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     4681 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     4741 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     4801 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     4861 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     4921 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     4981 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     5041 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     5101 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     5161 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     5221 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     5281 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     5341 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     5401 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     5461 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact
     5521 aatacgactc actataggga gacccaagct ggctaggtaa gcttggtacc gagctcggat
     5581 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     5641 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     5701 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
     5761 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     5821 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     5881 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     5941 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     6001 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     6061 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     6121 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     6181 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     6241 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     6301 gtggatctgg aggttctggt ggaatggcga gcccggcgcc tccggagcac gccgaggagg
     6361 gatgcccggc tcctgccgcc gaggagcagg cgccgccgtc gccgccaccg ccccaggcat
     6421 cccccgcaga gcggcagcag caggaggagg aagcgcagga agctggggcg gcggagggcg
     6481 cggggttgca ggtggaggag gccgcgggcc gggcggcggc cgcggtaacc tggctgctcg
     6541 gggagccggt gctgtggctg ggctgccgcg ccgacgagct gctgagctgg aagaggccgc
     6601 tgcggagcct gctcggcttc gtcgctgcca acctgctgtt ctggttcctt gcattgactc
     6661 catggagagt atatcacctg atttccgtca tgatacttgg gcgtgttatt atgcaaataa
     6721 taaaggatat ggttttgtct agaacaagag gtgcacagtt gtggagaagc ctcagtgaaa
     6781 gctgggaagt tatcaattcc aaaccagatg aaagacccag gctcagccac tgtattgcag
     6841 aatcatggat gaatttcagc atatttcttc aagaaatgtc tctttttaaa cagcagagcc
     6901 ctggcaagtt ttgtctcctg gtctgtagtg tgtgcacatt ttttacgatc ttgggaagtt
     6961 acattcctgg ggttatactc agctatctac tgttactgtg tgcatttttg tgtccattgt
     7021 ttaaatgtaa tgatattgga caaaaaattt acagcaaaat taagtcagtt ctgctgaaac
     7081 tggattttgg aattggagaa tatattaatc agaagaaacg tgagagatct gaagcagaca
     7141 aagaaaaaag tcacaaagat gacagtgaat tagacttttc agctctttgt cctaagatta
     7201 gcctcacggt tgctgccaaa gagttatctg tgtctgacac agacgtctca gaggtatcct
     7261 ggactgataa tgggaccttc aacctttcag aaggatacac tccacagaca gacacttctg
     7321 atgatcttga ccgacccagt gaggaagttt tctctagaga tctttcagat tttccatctc
     7381 tagaaaatgg catgggaaca aatgatgaag atgaattaag ccttggtttg cccactgagc
     7441 tcaagagaaa gaaggaacag ttggacagtg gtcacagacc aagcaaagag acgcaatcag
     7501 cagctggtct cacccttcct ctgaacagtg accaaacctt tcacctgatg agcaacctgg
     7561 ctggggatgt tatcacagct gcagtgactg cagctatcaa agaccagtta gagggtgtgc
     7621 agcaagcact ttctcaggct gcccccatcc cagaagagga cacagacact gaagaaggtg
     7681 atgactttga actacttgac cagtcagagc tggatcaaat tgagagtgaa ttgggactta
     7741 cacaagacca ggaagcagaa gcacagcaaa ataagaagtc ttcaggtttc ctttcaaatc
     7801 tgctgggagg ccattaatct agacccagct ttcttgtaca aagtggttga tatccagcac
     7861 agtggcggcc gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     7921 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     7981 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     8041 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     8101 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     8161 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     8221 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     8281 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     8341 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     8401 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     8461 ccccgcctgg acctgcagga ttctgcagat atccggacta gtgatctaat tctaccgggt
     8521 aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac
     8581 ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca
     8641 accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa
     8701 gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct
     8761 cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg
     8821 cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg
     8881 gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg
     8941 aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc
     9001 atctccgggc ctttcgacct gcagccaata tgggatcggc cattgaacaa gatggattgc
     9061 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     9121 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     9181 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     9241 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     9301 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     9361 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     9421 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     9481 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     9541 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     9601 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     9661 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     9721 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     9781 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgaacgcgtc
     9841 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag
     9901 ggcccggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     9961 aaagaaaagg ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt
    10021 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
    10081 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
    10141 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
    10201 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
    10261 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
    10321 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
    10381 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
    10441 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
    10501 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
    10561 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
    10621 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    10681 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    10741 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
    10801 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
    10861 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
    10921 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
    10981 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
    11041 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
    11101 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
    11161 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
    11221 aatattaacg cttacaattt
//
