LOCUS       sequence-205420-       10998 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic expression of luc, ekivR using P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10998)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 10998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS35.41
FEATURES             Location/Qualifiers
     source          1..10998
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          341..456
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             2223..2279
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(6333..6352)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6334..6352)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6359..6376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6359..6375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6368..6390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6517..6972
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6604..6623)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6814..6835
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6998..7102
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7103..7171,7172..7963)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7321..7340)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8134..8722
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8623..8642
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8876..8893
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9010..9031
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9046..9051,9052..9069,9070..9076)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9084..9100
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9089..9111
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9108..9124
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9108..9124
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        9145..9163
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     9191..9207
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     9192..9208
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          9772..9887
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(9905..10522,10523..10528,10529..10564,10565..10567,
                     10568..10603,10604..10606,10607..10642,10643..10651)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcc ccatcttcag
      301 tatattcatc ttcccatcca agaaccttta tttcccctaa gtaagtactt tgctacatcc
      361 atactccatc cttcccatcc cttattcctt tgaacctttc agttcgagct ttcccacttc
      421 atcgcagctt gactaacagc taccccgctt gagcagacat caccgtttaa acaccatgga
      481 ggacgccaag aacatcaaga agggccccgc ccccttctac cccctcgagg acggcaccgc
      541 cggcgagcag ctccacaagg ccatgaagcg ctacgccctc gtccccggca ccatcgcctt
      601 cgtaggtttc ctccagctct cgcctccagc acccgaggca cacctcgggc atcttcacaa
      661 caacagacac tgacatctca ttctcacaga ccgacgccca catcgaggtc aacatcacct
      721 acgccgagta cttcgagatg tccgtccgcc tcgccgaggc catgaagcgc tacggcctca
      781 acaccaacca ccgcatcgtc gtctgctccg agaactccct ccagttcttc atgcccgtcc
      841 tcggcgccct cttcatcggc gtcgccgtcg cccccgccaa cgacatctac aacgagcgcg
      901 agctcctcaa ctccatgaac atctcccagc ccaccgtcgt cttcgtctcc aagaagggcc
      961 tccagaagat cctcaacgtc cagaagaagc tccccatcat ccagaagatc atcatcatgg
     1021 actccaagac cgactaccag ggcttccagt ccatgtacac cttcgtcacc tcccacctcc
     1081 cccccggctt cagcgagtac gacttcgtcc ccgagtcctt cgaccgcgac aagaccatcg
     1141 ccctcatcat gaactcctcc ggctccaccg gcctccccaa gggcgtcgcc ctcccccacc
     1201 gcaccgcctg cgtccgcttc tcccacgccc gcgaccccat cttcggcaac cagatcatcc
     1261 ccgacaccgc catcctctcc gtcgtcccct tccaccacgg cttcggcatg ttcaccaccc
     1321 tcggctacct catctgcggc ttccgcgtcg tcctcatgta ccgcttcgag gaggagctct
     1381 tcctccgctc cctccaggac tacaagatcc agtccgccct cctcgtcccc accctcttct
     1441 ccttcttcgc caagtccacc ctcatcgaca agtacgacct ctccaacctc cacgagatcg
     1501 cctccggcgg cgcccccctc tccaaggagg tcggcgaggc cgtcgccaag cgcttccacc
     1561 tccccggcat ccgccagggc tacggcctca ccgagaccac ctccgccatc ctcatcaccc
     1621 ccgagggcga cgacaagccc ggcgccgtcg gcaaggtcgt ccccttcttc gaggccaagg
     1681 tcgtcgacct cgacaccggc aagaccctcg gcgtcaacca gcgcggcgag ctctgcgtcc
     1741 gcggccccat gatcatgtcc ggctacgtca acaaccccga ggccaccaac gccctcatcg
     1801 acaaggacgg ctggctccac tccggcgaca tcgcctactg ggacgaggac gagcacttct
     1861 tcatcgtcga ccgcctcaag tccctcatca agtacaaggg ctaccaggtc gcccccgccg
     1921 agctcgagtc catcctcctc cagcacccca acatcttcga cgccggcgtc gccggcctcc
     1981 ccgacgacga cgccggcgag ctccccgccg ccgtcgtcgt cctcgagcac ggcaagacca
     2041 tgaccgagaa ggagatcgtc gactacgtcg cctcccaggt caccaccgcc aagaagctcc
     2101 gcggcggcgt cgtcttcgtc gacgaggtcc ccaagggcct caccggcaag ctcgacgccc
     2161 gcaagatccg cgagatcctc atcaaggcca agaagggcgg caagtccaag ctcggctccg
     2221 gcgccaccaa cttctccctc ctgaagcagg ccggtgacgt cgaggagaac cccggcccca
     2281 tggaaaagcc tgtggcctca caagtccacc ccagctggct aacagctctt ctacctgaat
     2341 ccagaatacc ccccaagctg tttgcctggt cctcagccaa catccgccca gcaagctcct
     2401 ccaagctcgg aggctctgcc accaacatcc accatgcaga gtgttcacat ccaaccccaa
     2461 actccacagc aagctctgag cagcgcatct tcatcgttgg tcccggcaac ataggtaggc
     2521 tctacgcaag ccacatggtc cgccgcccaa atcccctccc catcactctc gtcgtcccca
     2581 gaaaggaact cctatcgcaa tgggcaggct gtgaaggcgt gggccttata gacccgggtc
     2641 gcggcaaggt cctcaaaagc aagcgcttca ccgtcgagtg gtggacggag actagaccac
     2701 agtatggtcc tgtgagagag gtggcagacg gaaagaagct gcgcaacgtg ttcatatcca
     2761 caaaggcatc cgctgggctg gccgaggcag atcgtcttcg tcggtatttg ggcaagtgta
     2821 gttctgtcgt ctttgcgcag aacggagtat gcaagctgtg gcctcctcat gggccgttat
     2881 acgtcgccgg tcgataccct cctggtgatg ctcctacctt ctcatcctgc gtcgtgaatc
     2941 atggcgttgc atcagcgggg ccttttctga gcattcacgc agctccggca gatgcatcca
     3001 tcggacctgt cctctggcct tcccatccga tgccgcacaa gaaacccctg aacgacttct
     3061 tcacaagata cattgctact acacccttcc taaacacaaa gcaagtttcc tctggtgagc
     3121 tctggcttat tcagctggag aaacttgtta ccaatgccgc catcaacccc ctcacagctt
     3181 tgctacgctg caaaacggga cagctgttca caacccatga ccccacagac cctcttacgc
     3241 gcgtgctgga caggttgcta tggcaggcca gcgccgtgat tcagggtctt atcaaccacg
     3301 acgcgagtgt cgacatcgtc gcttcctacg ccgagcacct ccaccagacc gaaccaagca
     3361 gcgactcaag ccttgtcgca gtacggacca agttaacaga gcggttctcc cagcctacct
     3421 tgagagcgaa gctattcgcc tttggtcgca agatatacga gcatcgcagc tcgatgcttc
     3481 aggatgttga ggccggcagg aaaacggaga taagagattt caacgggtgg gttgtggata
     3541 tggcgagttt tctggggacg gggctgaatg tgagcgtgca tcagagtttg attgtgctga
     3601 ttgaacgcgg tgaggttttg aacaaggatg agttggctag gaggacatta taagtttaaa
     3661 cagtagatgc cgaccgggat ccacttaacg ttactgaaat catcaaacag cttgacgaat
     3721 ctggatataa gatcgttggt gtcgatgtca gctccggagt tgagacaaat ggtgttcagg
     3781 atctcgataa gatacgttca tttgtccaag cagcaaagag tgccttctag tgatttaata
     3841 gctccatgtc aacaagaata aaacgcgttt cgggtttacc tcttccagat acagctcatc
     3901 tgcaatgcat taatgcattg gacctcgcaa ccctagtacg cccttcaggc tccggcgaag
     3961 cagaagaata gcttagcaga gtctattttc attttcggga gacgagatca agcagatcaa
     4021 cggtcgtcaa gagacctacg agactgagga atccgctctt ggctccacgc gggcgcgcct
     4081 cggtcgctca ctgttccttt acggatatgt gtactccggc aagccgcggg gtcgcggtcg
     4141 tttgtacggc agcttctact tgcttgcaca gggagctctc tggggcttga cgtcttttgg
     4201 agttgcgagg gagttgattt cctacttcta agtttggact gaatccgtgg tgtgattgag
     4261 gtgattggcg atgtttggct ataccagcta tatgtaataa tctctactgt atactactat
     4321 tcaacgcatt ttactatgcg tgctgctagg gtcggcaatg acaatggcaa tctgactgac
     4381 gtggtctatt tctccatgtg cagcagggaa tacgagctcc aatggacctc gggagtggca
     4441 cagtcaatgg caaggaaact ccgcctttgc aggtgtggct gaaccccacg ggtcggaggc
     4501 ggagcaatcc acccccgatg tggctggtgc gtggaggggc tcgcgatgat tttactgagc
     4561 ttgcttttct tgtcgacatt gaacattgtc cttggtcttc cttcagattt aagggtcagt
     4621 cactgctaca tttctcagta gtatccgcgc acgtctctgg atttacgaat cagggtccac
     4681 cagtcgaaac ttcgaactac tctcattata caatcctctt tccattcccg cattaacccc
     4741 tccatcaaca ccatgtcctc caagtcgcaa ttgacctaca ctgcccgtgc cagcaagcat
     4801 cccaatgctc tggcgaagag gctgttcgag attgccgagg ccaagaagac caatgtgact
     4861 gtctcggctg acgttaccac cactaaggag ctactagatc ttgctgaccg taggccgacc
     4921 cgctactctg cctgattatg ctgcatgcaa acttattaac ggtgataccg gactgcaggt
     4981 ctcggtccct acattgccgt gatcaaaacc cacatcgata tcctctctga tttcagcaac
     5041 gagaccattg agggacttaa ggctctcgcg cagaagcaca actttctcat cttcgaggac
     5101 cgcaagttca ttgacatcgg caacacggtc cagaagcaat accacggcgg taccctccgt
     5161 atctcggaat gggcccacat catcaactgc agcattctcc ctggtgaggg tatcgtcgag
     5221 gctctcgctc agacggcgtc tgcaccggac ttcgcatacg gccccgaacg cggtctgttg
     5281 atcttggcag agatgacctc taagggctcc ttggctaccg gccagtacac tacttcctcg
     5341 gtcgattatg cccggaaata caagaacttc gttatgggat tcgtgtcgac gcgcgcgttg
     5401 ggtgaggtgc agtcggaagt cagctctcct tcggatgagg aggactttgt ggtcttcacg
     5461 actggtgtga acatttcttc caagggagat aagcttggtc agcagtacca gacgcccgga
     5521 tcggctatcg gccggggtgc tgacttcatt atcgcgggtc gcggtatcta cgccgcgccg
     5581 gatcgatccg gtgcaggctg cgcaacagta tcagaaggag gggtgggaag cctacctggc
     5641 ccgtgtcggc ggaaactaat actataaaag gaggatcgaa gttctgatgg ttatgaatga
     5701 tatagaaatg caacttgccg caacggatac ggaagcggaa acggaccaat gtcgagcacg
     5761 ggtagtcaga ctgcggcatc ggatgtccaa acggtattga tcctgcaggc tactatggtg
     5821 tggcacaagg atcaatgcgg tacgacgatt tgatgcagat aagcaggctg cgaagtagta
     5881 actcttgcgt agagaaaatg gcgacgggtg gctgataagg gcggtgataa gcttaattgt
     5941 catcgcagat aagcactgct gtcttgcatc caagtcagcg tcagcagaaa tacgggactt
     6001 ccgaaagtat atggcaaaat taaagaactt gactctccag caatgttttg ccctgaccgt
     6061 cgctaaaacg ttactacccc tatacccgtc tgtttgtccc agcccgaggc attaggtctg
     6121 actgacagca cggcgccatg cgggcttggg acgccatgtc cgtcgcgtga taagggttga
     6181 tccatgcagc tactatcctt ccatcgttcc attcccatcc ttgtcctatc tccatccttg
     6241 aaactttact agtttagttg gatgctcgag cttgctctcg gctactccgt cggcgcgcct
     6301 tgcggccgcc accgcggtgg agctccaatt cgccctatag tgagtcgtat tacaattcac
     6361 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6421 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6481 cttcccaaca gttgcgcagc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa
     6541 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6601 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6661 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6721 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6781 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6841 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6901 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6961 cgcttacaat ttccattcgc cattcaggct gmrtgtgcgc ggaaccccta tttgtttatt
     7021 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     7081 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     7141 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     7201 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7261 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7321 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7381 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7441 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7501 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7561 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7621 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7681 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7741 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7801 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7861 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7921 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7981 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     8041 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     8101 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     8161 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     8221 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8281 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8341 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8401 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8461 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8521 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8581 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8641 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8701 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8761 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8821 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8881 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8941 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     9001 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     9061 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     9121 tgaccatgat tacgccaagc tcgaaattaa ccctcactaa agggaacaaa agctggtacc
     9181 gggccccccc tcgaggtcga cggtatcgat aagcttcgga gaatatggag cttcatcgaa
     9241 tcaccggcag taagcgaagg agaatgtgaa gccaggggtg tatagccgtc ggcgaaatag
     9301 catgccatta acctaggtac agaagtccaa ttgcttccga tctggtaaaa gattcacgag
     9361 atagtacctt ctccgaagta ggtagagcga gtacccggcg cgtaagctcc ctaattggcc
     9421 catccggcat ctgtagggcg tccaaatatc gtgcctctcc tgctttgccc ggtgtatgaa
     9481 accggaaagg ccgctcagga gctggccagc ggcgcagacc gggaacacaa gctggcagtc
     9541 gacccatccg gtgctctgca ctcgacctgc tgaggtccct cagtccctgg taggcagctt
     9601 tgccccgtct gtccgcccgg tgtgtcggcg gggttgacaa ggtcgttgcg tcagtccaac
     9661 atttgttgcc atattttcct gctctcccca ccagctgctc ttttcttttc tctttctttt
     9721 cccatcttca gtatattcat cttcccatcc aagaaccttt atttccccta agtaagtact
     9781 ttgctacatc catactccat ccttcccatc ccttattcct ttgaaccttt cagttcgagc
     9841 tttcccactt catcgcagct tgactaacag ctaccccgct tgagcagaca tcaccgaatt
     9901 caccatgtct agactggaca agagcaaagt cataaacggc gctctggaat tactcaatgg
     9961 agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc
    10021 taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat
    10081 gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc aagactttct
    10141 gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg gggctaaagt
    10201 gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt
    10261 cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca
    10321 ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga
    10381 gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc tgttcgaccg
    10441 gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa
    10501 acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg acttagacat
    10561 gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga
    10621 cgattttgac cttgacatgc tccccgggta actaagtaag gatccactag tacagcagaa
    10681 gaatctctct ccgctgttgc ttcagtgtct gccatgcatt aacttcatcc tactgtccta
    10741 cccgcagtac ccattcacat ttgcggcaga taccaggttg ttttctattc cctcggttct
    10801 tcagttcttc agatattaca ttactcgaga aattggcggc gatgcaggag tttggttggt
    10861 tttaattgtt tcagtctcct tgacctgatt tgtatgacat gcaatgtttc gacggaagac
    10921 tatctcggtg aatatacgtc atgaatcatg cggccgcgcg tatcacgagg ccctttcgtc
    10981 ttcactcgag tttaccac
//
