LOCUS       sequence-205421-       20460 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Polycistronic expression of ekivR, esyn1, luc using P2A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20460)
  AUTHORS   Schuetze T, Meyer V
  TITLE     Polycistronic gene expression in Aspergillus niger.
  JOURNAL   Microb Cell Fact. 2017 Sep 25;16(1):162. doi: 
            10.1186/s12934-017-0780-z.
  PUBMED    28946884
REFERENCE   2  (bases 1 to 20460)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2017-09-25"; volume: "16(1):162. doi"; pages: " 
            10.1186/s12934-017-0780-z"
COMMENT     Sequence Label: pTS38.3
FEATURES             Location/Qualifiers
     source          1..20460
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    join(1..19,20..42,43..61,62..84,85..103,104..126,127..145,
                     146..168,169..187,188..210,211..229,230..252,253..271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     intron          341..456
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             1856..1912
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             11318..11374
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(15795..15814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(15796..15814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(15821..15838)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(15821..15837)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15830..15852)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(15978..16433)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(16115..16136)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     16327..16346
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        16460..16564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(16565..16633,16634..17425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(16783..16802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      17596..18184
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     18085..18104
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     18338..18355
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    18472..18493
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(18508..18513,18514..18531,18532..18538)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    18546..18562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     18551..18573
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     18570..18586
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     18570..18586
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        18607..18625
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     18653..18669
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     18654..18670
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          19234..19349
                     /gene="gpdA"
                     /label=gpdA intron
                     /note="intron from the Aspergillus nidulans 
                     glyceraldehyde-3-phosphate dehydrogenase gene (Punt et al.,
                     1990)"
     CDS             join(19367..19984,19985..19990,19991..20026,20027..20029,
                     20030..20065,20066..20068,20069..20104,20105..20113)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
ORIGIN
        1 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
       61 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
      121 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
      181 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
      241 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagctcc ccatcttcag
      301 tatattcatc ttcccatcca agaaccttta tttcccctaa gtaagtactt tgctacatcc
      361 atactccatc cttcccatcc cttattcctt tgaacctttc agttcgagct ttcccacttc
      421 atcgcagctt gactaacagc taccccgctt gagcagacat caccgtttaa acaccatgga
      481 aaagcctgtg gcctcacaag tccaccccag ctggctaaca gctcttctac ctgaatccag
      541 aatacccccc aagctgtttg cctggtcctc agccaacatc cgcccagcaa gctcctccaa
      601 gctcggaggc tctgccacca acatccacca tgcagagtgt tcacatccaa ccccaaactc
      661 cacagcaagc tctgagcagc gcatcttcat cgttggtccc ggcaacatag gtaggctcta
      721 cgcaagccac atggtccgcc gcccaaatcc cctccccatc actctcgtcg tccccagaaa
      781 ggaactccta tcgcaatggg caggctgtga aggcgtgggc cttatagacc cgggtcgcgg
      841 caaggtcctc aaaagcaagc gcttcaccgt cgagtggtgg acggagacta gaccacagta
      901 tggtcctgtg agagaggtgg cagacggaaa gaagctgcgc aacgtgttca tatccacaaa
      961 ggcatccgct gggctggccg aggcagatcg tcttcgtcgg tatttgggca agtgtagttc
     1021 tgtcgtcttt gcgcagaacg gagtatgcaa gctgtggcct cctcatgggc cgttatacgt
     1081 cgccggtcga taccctcctg gtgatgctcc taccttctca tcctgcgtcg tgaatcatgg
     1141 cgttgcatca gcggggcctt ttctgagcat tcacgcagct ccggcagatg catccatcgg
     1201 acctgtcctc tggccttccc atccgatgcc gcacaagaaa cccctgaacg acttcttcac
     1261 aagatacatt gctactacac ccttcctaaa cacaaagcaa gtttcctctg gtgagctctg
     1321 gcttattcag ctggagaaac ttgttaccaa tgccgccatc aaccccctca cagctttgct
     1381 acgctgcaaa acgggacagc tgttcacaac ccatgacccc acagaccctc ttacgcgcgt
     1441 gctggacagg ttgctatggc aggccagcgc cgtgattcag ggtcttatca accacgacgc
     1501 gagtgtcgac atcgtcgctt cctacgccga gcacctccac cagaccgaac caagcagcga
     1561 ctcaagcctt gtcgcagtac ggaccaagtt aacagagcgg ttctcccagc ctaccttgag
     1621 agcgaagcta ttcgcctttg gtcgcaagat atacgagcat cgcagctcga tgcttcagga
     1681 tgttgaggcc ggcaggaaaa cggagataag agatttcaac gggtgggttg tggatatggc
     1741 gagttttctg gggacggggc tgaatgtgag cgtgcatcag agtttgattg tgctgattga
     1801 acgcggtgag gttttgaaca aggatgagtt ggctaggagg acattaggct ccggcgccac
     1861 caacttctcc ctcctgaagc aggccggtga cgtcgaggag aaccccggcc ccatgtcact
     1921 ccacacccca agtgacggtc aacaagaccc agcgttagcc tccaaaaccc tctgtgagca
     1981 aatctcgcga gccctcggcc tcggccagga caagattgag aatatcttcc cagggacacc
     2041 cttccagcgg gacgtcatag actgcgccgc agacgacaag cagcgcgccg tgggccacgc
     2101 tgtctttgaa attcccaaag atatagacgc ggcccgcctg gcggctgcgt ggaaggagac
     2161 cgtgcttcac acccctgctc tgcggacctg cacgtttacg tccaaatctg gggatgtgct
     2221 tcaggtggtc ctgagagata gctttgtctt ttcgtggatg tctgggccgt ctgtggatct
     2281 taaggaggcg gttgtgcaag acgaggctgc tgctgctttg gctggcccgc gctgtaaccg
     2341 cttcgtcctt cttgaggacc ctgatacaaa ggaacgtcag ctcatctgga cgttcagcca
     2401 tgctctcgtt gatagtacct tccaggaacg tatccttcga cgagtcctca aagcttacaa
     2461 ggatgccaat gacgaacatc ctcgccagtt cgagacacca gattcttccc aggccacgcc
     2521 cgaggaggat ttacagccca atccatccaa gatgctcaag atccctcaag ccgcagatat
     2581 ggaccgtgct gttgagtttt ggaaggatca tctaagtggc ctgaatgctt ctgccttccc
     2641 gcatttgtcc tctcatctgt ctatgccaca tccagatgcc aaggcagagc atagaatctc
     2701 ctactcgtct tcagcacagc aaaaaatgtc cagtgctact atttgccgaa ctgccctggc
     2761 aattctgtta tctcgttata cgcactctcc tgaagcactc tttggcattg taactgagca
     2821 aactcctcta ctggaggagc aactcatgct tgatggccca acacgtacag tcgtaccaat
     2881 ccgcgtatcc tgtgcttctg aacagtcagt gtcagatatc atgagcacga ttgactccta
     2941 cgatcagacc atgcggcagt ttgcccacgc tggtcttcgc aacatcgcca gtgctggaga
     3001 cgatgaatct gccgcctgcg ggttccagac cgttctcttg gtctcagatg gagacgccca
     3061 gccagcatct acttgggaaa ttctcaagaa aactgaagag cctgagggtt tcatcccctg
     3121 tacgaaccgt gctctgcttc taagttgcca gatgaccagc tctggagcac acctaaccgc
     3181 cagatacgac cagagtatca tcgacgctga gcaaatggcc cgactcctgc gacaactagg
     3241 ccatctgatc caaaatcttc aaacctccac agatctaccc gtggaaaagg tggacatgat
     3301 gacgcaagaa gactggttag agattgagag gtggaactca gactccatcg acgcacaaga
     3361 cacgcttatc cacagcgaga tgttgaagtg gacttctcag tcgcctaaca aagctgccgt
     3421 cgccgcgtgg gatggagagt ggacgtatgc tgagctggac aatgtatctt cgcgcctggc
     3481 tcagcatatc aactctattg acctgggcaa ggagcacgcg atagtaccca tctactttga
     3541 gaaatcgaaa tgggtcgttg cttcgatgct ggctgtgctc aaggctggcc atgcgtttac
     3601 tctcattgac cccagtgatc caccagctcg aacagcacag gtcgtgcagc aaacctctgc
     3661 gacagttgct cttacttcca agctccatcg cgagactgtt caatcaactg tcgggcgctg
     3721 cattgttgtc gacgaagagt ttgtcaaatc cctcccccag tccagcgaac tctcagcgtc
     3781 tgtaaaggca catgacctgg catatgtcat cttcacctcg ggcagcacag gcattccaaa
     3841 gggaatcatg attgagcacc gctcattttc atcctgcgcc atcaaattcg gtcccgcgct
     3901 tggaataacc tccgacacac gcgctcttca gtttggatct catgcctttg gcgcgtgtat
     3961 cctcgagatc atgacgacgc tcatccacgg aggctgtgtc tgtatcccct ctgacgatga
     4021 ccgcatgaac aacgtccttg agttcatcaa ccggactaac gtcaactggg tcatggcaac
     4081 gccttcgtac atggggacat tccagccaga ggttgttcct ggtttgaaga ccttggtatt
     4141 ggtaggtgag cagatgtctg cttctgtgaa tgaggtctgg gctcctcgtg ttcaactcct
     4201 gaacggttac ggacagagcg agagttcttc tatctgctgc gtagccaaga tcagccctgg
     4261 ttcatctgag ccaaacaata ttggccacgc agtaggcgca cattcttgga tcgttgaccc
     4321 tgaggatccc aatcgcttgg ctcccatcgg tgccgttggc gagctggtca tcgagagtgc
     4381 aggtatcgca cgcgattaca tcgttgcgcc aactcaggat aagtctcctt tcattaagac
     4441 tgctcctacg tggtatccgg ccaagcagct cccggacggc ttcaagatct acagaacagg
     4501 cgatcttgca tgctacgcat ctgatggcag tatagtgtgc cttggccgta tggactccca
     4561 ggtcaagatc agaggacagc gtgttgagct gggtgcagtg gagactcatc ttcgacagca
     4621 gatgcctgat gatatgacca tcgttgttga agctgtcaag ttctctgatt cctccagtac
     4681 tacagttttg acagccttct tgattggtgc aggggagaag aacagtcata tcttggacca
     4741 gcgtgctaca agggagatca atgccaagat ggagcaagtt cttcctcgcc attccatccc
     4801 cgcgttctac atctctatga acaacctccc ccaaacagcc actggtaagg tcgatcgacg
     4861 aaaacttcgt atcatgggta gcaagatact gagccaaaag acgcattcca ccccatctca
     4921 gcagagtcag gccgccatct catctggaac cgacacagag accaagctgg agagcatctg
     4981 gatcacaagc ttggacctcg agcccggctc tgcaaacatg tcagctacat tcttcgagat
     5041 gggcggaaac tccatcatcg caatcaagat ggtcaacatg gcgcgctcca atggcataga
     5101 gcttaaggtc tcggacattt accagaaccc cacgctcgct ggtctcaagg ctattgtcat
     5161 tggtacttcg ctgccataca gccttattcc caaggttacc cgccaaggcc ctgttagcga
     5221 gcagtcttat gcgcaaaaca gaatgtggtt cctggatcag ttgtctgagg gtgcttcatg
     5281 gtatctgatt cctttcgctg tgcgcatgcg aggtccggtg gatgttgatg cgttgacgcg
     5341 tgctttgctc gctcttgaac agcgtcatga gacactacga actacatttg agaaccaaga
     5401 tggtgttgga gtccagatca tccatgatag actctccaaa gagctacaag tcatcgatgc
     5461 cttggatggt gacgagggtg gtctcaagac actctacaaa gtagagacca ccacattcga
     5521 catcacatcc gaagcaggct ggagctcaac cctcatccgc ctcggcaaag acgatcacat
     5581 tctgtctatc gtcatgcacc acatcatctc cgacggctgg tccatcgacg ttctccgccg
     5641 cgaactcatc caactctacg ccgccgctct gcagggcaag gatccttcct ccgcactaac
     5701 ccccctaccc atccagtaca gcgacttcgc cgtgtggcag aagcaggagg cccaagcagc
     5761 tgagcacgag aggcagctcc agtactggaa gaagcaactc gcagatagtt cacctgccaa
     5821 gatccctacc gacttccccc gtccagatct cctgtccggt gacgcaggcg ttgtgcccgt
     5881 tgccatcgac ggcgagctgt atcagaaact aaggggcttc tgcaacaagc acaacagcac
     5941 tgccttctcc atcctgctcg ctgctttccg cgcggcgcat taccgtctca cagccgttga
     6001 cgacgccgtg atcggcatcc ccattgcaaa ccgtaaccgc tgggagctgg agaacatgat
     6061 tggtttcttt gtcaacacgc agtgtatgcg catcgccgtt gacgagacgg atacatttga
     6121 gagtctggtg cgccaggtca gatctaccac tacagctgcg tttgcgcacg aggatgtccc
     6181 cttcgagcgt gtcgtttcag cgcttcagcc tggccataga gatctctcgc gaacaccgct
     6241 ggcacagata atgtttgctg ttcactcgca gaaggacctt ggacgtttcg agctggaggg
     6301 tatccagtct gagcctatcg ccagcaaggc ctacaccaga ttcgatgtcg agttccatct
     6361 gttccaacag gcagacggac tgaagggcag ttgcaacttt gccacagatc tcttcaagcc
     6421 cgaaactatc cagaatgttg tcagcgtgtt tttccagatt ctacgccatg gccttgacca
     6481 gcctgagacg tgtatctcgg ttcttccact gactgatgga gtcgaggagc ttcgcaggtt
     6541 ggatctgctg gaaatcaaga ggactaacta ccctcgcgat tcgagcgtgg tagatgtctt
     6601 ccgcgaacaa gctgccgcca acccagaggt tatcgctgtt accgactcat cttctcgtct
     6661 gacttatgca gagctggaca ataagtctga gctgctctca cgatggcttc gacgacgtaa
     6721 cttgacgcca gagacgctgg tcagtgttct tgctccccgg tcttgcgaga ctatcgttgc
     6781 ctatgttggt atcctcaagg cgaacctggc gtatcttcct cttgacgtga ggtccccggt
     6841 gactcgtatg aaggacatct tgtcgagcgt gtctggaaac accatagttc ttatgggctc
     6901 tggggtagag gatcctggct ttgacttacc gcaactagag ctcgtacgca tcaccgacac
     6961 tttcgatgag accatcgagg acgtgcaaga ctctccccaa ccgtctgcca caagcctcgc
     7021 ctacgtcgtc ttcacatccg gttcaactgg taaacccaag ggcgtcatga tcgagcaccg
     7081 ggccattgtg cgtctcgtca agagtgacaa ctttcctggc ttcccctccc ccgctcgcat
     7141 gtcaaatgtc ttcaaccctg ccttcgacgg agccatctgg gagatcaact ggatgctcct
     7201 gaacggcgga acagtcgtct gcatcgacta cctgaccacc ctggacggca aagagctcgc
     7261 tgctgtgttc gccaaagagc gcgtcaacgc cgccttcttc gcacctgcga tgctcaagct
     7321 ttacctcgtt gatgcgcgcg aggctttgaa gaatcttgac ttccttattg ttggaggtga
     7381 gaggttcgat accaaggaag ccgtggaggc catgccgctt gtgagaggca agattgccaa
     7441 tatctatggt ccgactgagg ctggaataat cagcacgtgc tataacatcc ccaaggatga
     7501 ggcttacacc aatggtgttc ccattggtgg aagtatctac aactctggtg cctacgtcat
     7561 ggatcctaac cagcaacttg tcggccttgg cgtcatggga gagcttgttg ttaccggaga
     7621 cggtgttggt cgaggctaca ctaaccccga gctcaacaag aaccgcttca tcgacatcac
     7681 catcgagggc aagactttca aggcttaccg tactggtgac cggatgcgtg cacgagtagg
     7741 cgacggtctc cttgagttct tcggccgcat ggacaaccag ttcaagatcc gcggcaaccg
     7801 tatcgaagca ggggaagttg agtctgccat gctcagcctc aagaatgtcc ttaacgccgc
     7861 cattgtcgtc cgcgggggcg gagaagatga agggccactc gagatggtcg gattcatcgt
     7921 cgcggacgac aagaatgata ccacggagga ggaagagaca ggcaaccaag ttgagggctg
     7981 gcaggaccat ttcgagagtg gtatgtactc ggatatcagc accgccgtgg accaatctgc
     8041 cattggaaac gactttaagg gctggacttc tatgtacgat ggtaaggata tcgacaaggg
     8101 cgagatgcag gagtggttgg acgacgctat tcacaccctg cataacggcc agatccctcg
     8161 cgatgtcctc gagatcggta ccggtagtgg tatgatcctg ttcaacctca acccgggcct
     8221 caacagctac gttggtcttg atccatccaa gtcagcagtc gagttcgtca acagagccgt
     8281 cgagtcctct cccaagttcg caggaaaagc caaggtccac gtcggcatgg ccacagacgt
     8341 caacaaactc ggcgaagtac accccgatct cgtggtcttc aactccgttg ttcaatactt
     8401 ccccacaccc gagtatctcg ccgaggtcat cgatggcctc attgccatcc ccagcgtcaa
     8461 gcgcatcttc cttggcgata tcagatcata tgctaccaac ggacacttcc tcgccgcacg
     8521 cgccatccac acgctcggca ccaataacaa cgccaccaag gatagggtgc gccagaagat
     8581 ccaggagctg gaggatcgag aggaagagtt tctcgttgag cctgccttct tcaccactct
     8641 gaaggagcga cgtccagatg ttgtcaagca tgttgagatc atccccaaga acatgaaggc
     8701 caccaacgaa ctcagcgcct atcgctacac ggctgttgtg catctgcggg atgaaacgga
     8761 cgaacctgtg tatcatattg agaaggatag ctgggttgac tttgaggcga agcagatgga
     8821 caagacggct cttcttgacc acctgcgcct ctccaaggat gctatgagtg tggcggttag
     8881 caacatcacc tacgcccaca ctgcctttga acgtcgtatc gttgagtctc tcgatgagga
     8941 tagcaaggat gacaccaagg gtacactcga tggtgcagcc tggctctcag cagttcgctc
     9001 cgaagccgaa aaccgcgcct cactcaccgt ccccgacatc ctggagatcg ccaaagaggc
     9061 tggtttccga gttgaagtca gcgctgctcg ccagtggtcc caaagtggtg ctttagacgc
     9121 agtcttccac cactttccac cctccagcac tgaccgcact ctaatccagt tccccacgga
     9181 caacgagctt cgatcatcac tcaccctcgc caaccgccct ctccagaagc tgcagcgccg
     9241 tcgtgccgct ctgcaagtcc gcgagaagct ccagacgctg gtcccgtctt acatggttcc
     9301 tccgaatatc gtggtgctgg acacgatgcc tctcaatact aacggcaaga tcgacagaaa
     9361 ggagcttacg cgtagagcac gaacactgcc gaagcagcag actgcggcgc ctgtgccgga
     9421 cttccctatc tctgatatcg agatcacgct gtgcgaggag gcaactgagg tctttggaat
     9481 gaaggttgaa atcagcgatc acttcttcca gctcggcggt cactctctcc tcgctacgaa
     9541 actcatttct cgcatccagc accgtctcca tgtgcgggtt actgtgaagg acgtattcga
     9601 cagccctgtc tttgccgatc tggcagtcat catccgtcaa ggacttgcta tgcagaaccc
     9661 tgttgctgaa ggacaggaca agcaaggctg gtcctcgaga gttgcccctc gtacagaagt
     9721 cgagaagatg ctgtgcgagg agttcgcagc tggtcttggt gtcccggttg gtatcactga
     9781 caacttcttc gatctcggtg gtcactcgct catggctact aagctagctg tgcgaattgg
     9841 ccgtcgcctt gataccgcca tcacagtcaa ggacatcttc gactaccctg tgcttttcca
     9901 attggcgaag aagttggagt cttcgcattc caagagctac gaggagtctg gcgacgatat
     9961 ccagatggcc gattacactg cattccagct cctcgatctg gaagaccccc aagactttgt
    10021 tcagtcccag attcggcctc aactggactc ctgctacggc accatccagg atgtctaccc
    10081 gtctacgcag atgcaaaagg ccttcctctt cgatcccacg accggcgagc cccgaggtct
    10141 tgtgcctttc tatatcgact tccccagcaa tgcagatgcc gagaccctca ccaaggctat
    10201 cggagctcta gttgacaagc tcgatatgtt cagaactgtc ttcctggagg ccgcaggcga
    10261 tctgtaccaa gttgtcgttg agcacctcaa cttgcctatt gagaccatcg agactgagaa
    10321 gaacgtcaac actgcaaccg gtgactacct ggatgttcat ggaaaggacc ctgtccgtct
    10381 aggccacccg tgcatccaat tcgccatcct gaagactgcc tcctctgtac gtgttctcct
    10441 gcgaatgtcc cacgctctgt atgatggttt gagttttgag tacatcgtcc gtggtctcca
    10501 cgttctctac agcggtagaa acctcccccc accaacacag tttgcgcgat acatgcagta
    10561 tgctgcacac agtcgtgaag aaggttatcc cttctggcgc gaggttctgc agaacgcgcc
    10621 catgacagtt ctacacgaca ccaataacgg tatgtctgag caagagatgc cagcctccaa
    10681 ggcggtacac ctgtcagagg tcgtcaacgt tccagcgcaa gctattcgaa acagtaccaa
    10741 cacccaagcg accgtcttca acaccgcctg tgcccttgtc ctagccaagg aatccggctc
    10801 acaggatgtc gtcttcggcc gtattgtctc tggtcgacaa ggtctaccag tcgtctggca
    10861 ggacatcatc ggcccctgca caaacgccgt gcccgtccac gcacgcgtcg acgatggaaa
    10921 cccccaacgc atcatccgcg acctacgcga ccaatacctc cgcactctgc ccttcgaatc
    10981 gcttggcttc gaggaaatca agcgtaactg cacggactgg cccgaagaat tgaccaactt
    11041 ctctgtctgc gtgacgtacc acaacttcga gtaccacccc gagagcgaag ttgacaacca
    11101 aaaggttgag atgggagttt tggcaaagta tgttgagttg agtgaaaacg agccgctgta
    11161 cgatcttgct attgcgggag aggttgaggc ggatggagtt aacctgaagg tcactgttgt
    11221 tgcaaaggca aggctttaca atgaggcgag gattagacat gtgcttgagg aagtttgcaa
    11281 aactttcaat ggtttgaacg aggctttggg ctccggcgcc accaacttct ccctcctgaa
    11341 gcaggccggt gacgtcgagg agaaccccgg ccccatggag gacgccaaga acatcaagaa
    11401 gggccccgcc cccttctacc ccctcgagga cggcaccgcc ggcgagcagc tccacaaggc
    11461 catgaagcgc tacgccctcg tccccggcac catcgccttc gtaggtttcc tccagctctc
    11521 gcctccagca cccgaggcac acctcgggca tcttcacaac aacagacact gacatctcat
    11581 tctcacagac cgacgcccac atcgaggtca acatcaccta cgccgagtac ttcgagatgt
    11641 ccgtccgcct cgccgaggcc atgaagcgct acggcctcaa caccaaccac cgcatcgtcg
    11701 tctgctccga gaactccctc cagttcttca tgcccgtcct cggcgccctc ttcatcggcg
    11761 tcgccgtcgc ccccgccaac gacatctaca acgagcgcga gctcctcaac tccatgaaca
    11821 tctcccagcc caccgtcgtc ttcgtctcca agaagggcct ccagaagatc ctcaacgtcc
    11881 agaagaagct ccccatcatc cagaagatca tcatcatgga ctccaagacc gactaccagg
    11941 gcttccagtc catgtacacc ttcgtcacct cccacctccc ccccggcttc agcgagtacg
    12001 acttcgtccc cgagtccttc gaccgcgaca agaccatcgc cctcatcatg aactcctccg
    12061 gctccaccgg cctccccaag ggcgtcgccc tcccccaccg caccgcctgc gtccgcttct
    12121 cccacgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgcc atcctctccg
    12181 tcgtcccctt ccaccacggc ttcggcatgt tcaccaccct cggctacctc atctgcggct
    12241 tccgcgtcgt cctcatgtac cgcttcgagg aggagctctt cctccgctcc ctccaggact
    12301 acaagatcca gtccgccctc ctcgtcccca ccctcttctc cttcttcgcc aagtccaccc
    12361 tcatcgacaa gtacgacctc tccaacctcc acgagatcgc ctccggcggc gcccccctct
    12421 ccaaggaggt cggcgaggcc gtcgccaagc gcttccacct ccccggcatc cgccagggct
    12481 acggcctcac cgagaccacc tccgccatcc tcatcacccc cgagggcgac gacaagcccg
    12541 gcgccgtcgg caaggtcgtc cccttcttcg aggccaaggt cgtcgacctc gacaccggca
    12601 agaccctcgg cgtcaaccag cgcggcgagc tctgcgtccg cggccccatg atcatgtccg
    12661 gctacgtcaa caaccccgag gccaccaacg ccctcatcga caaggacggc tggctccact
    12721 ccggcgacat cgcctactgg gacgaggacg agcacttctt catcgtcgac cgcctcaagt
    12781 ccctcatcaa gtacaagggc taccaggtcg cccccgccga gctcgagtcc atcctcctcc
    12841 agcaccccaa catcttcgac gccggcgtcg ccggcctccc cgacgacgac gccggcgagc
    12901 tccccgccgc cgtcgtcgtc ctcgagcacg gcaagaccat gaccgagaag gagatcgtcg
    12961 actacgtcgc ctcccaggtc accaccgcca agaagctccg cggcggcgtc gtcttcgtcg
    13021 acgaggtccc caagggcctc accggcaagc tcgacgcccg caagatccgc gagatcctca
    13081 tcaaggccaa gaagggcggc aagtccaagc tctaagttta aacagtagat gccgaccggg
    13141 atccacttaa cgttactgaa atcatcaaac agcttgacga atctggatat aagatcgttg
    13201 gtgtcgatgt cagctccgga gttgagacaa atggtgttca ggatctcgat aagatacgtt
    13261 catttgtcca agcagcaaag agtgccttct agtgatttaa tagctccatg tcaacaagaa
    13321 taaaacgcgt ttcgggttta cctcttccag atacagctca tctgcaatgc attaatgcat
    13381 tggacctcgc aaccctagta cgcccttcag gctccggcga agcagaagaa tagcttagca
    13441 gagtctattt tcattttcgg gagacgagat caagcagatc aacggtcgtc aagagaccta
    13501 cgagactgag gaatccgctc ttggctccac gcgggcgcgc ctcggtcgct cactgttcct
    13561 ttacggatat gtgtactccg gcaagccgcg gggtcgcggt cgtttgtacg gcagcttcta
    13621 cttgcttgca cagggagctc tctggggctt gacgtctttt ggagttgcga gggagttgat
    13681 ttcctacttc taagtttgga ctgaatccgt ggtgtgattg aggtgattgg cgatgtttgg
    13741 ctataccagc tatatgtaat aatctctact gtatactact attcaacgca ttttactatg
    13801 cgtgctgcta gggtcggcaa tgacaatggc aatctgactg acgtggtcta tttctccatg
    13861 tgcagcaggg aatacgagct ccaatggacc tcgggagtgg cacagtcaat ggcaaggaaa
    13921 ctccgccttt gcaggtgtgg ctgaacccca cgggtcggag gcggagcaat ccacccccga
    13981 tgtggctggt gcgtggaggg gctcgcgatg attttactga gcttgctttt cttgtcgaca
    14041 ttgaacattg tccttggtct tccttcagat ttaagggtca gtcactgcta catttctcag
    14101 tagtatccgc gcacgtctct ggatttacga atcagggtcc accagtcgaa acttcgaact
    14161 actctcatta tacaatcctc tttccattcc cgcattaacc cctccatcaa caccatgtcc
    14221 tccaagtcgc aattgaccta cactgcccgt gccagcaagc atcccaatgc tctggcgaag
    14281 aggctgttcg agattgccga ggccaagaag accaatgtga ctgtctcggc tgacgttacc
    14341 accactaagg agctactaga tcttgctgac cgtaggccga cccgctactc tgcctgatta
    14401 tgctgcatgc aaacttatta acggtgatac cggactgcag gtctcggtcc ctacattgcc
    14461 gtgatcaaaa cccacatcga tatcctctct gatttcagca acgagaccat tgagggactt
    14521 aaggctctcg cgcagaagca caactttctc atcttcgagg accgcaagtt cattgacatc
    14581 ggcaacacgg tccagaagca ataccacggc ggtaccctcc gtatctcgga atgggcccac
    14641 atcatcaact gcagcattct ccctggtgag ggtatcgtcg aggctctcgc tcagacggcg
    14701 tctgcaccgg acttcgcata cggccccgaa cgcggtctgt tgatcttggc agagatgacc
    14761 tctaagggct ccttggctac cggccagtac actacttcct cggtcgatta tgcccggaaa
    14821 tacaagaact tcgttatggg attcgtgtcg acgcgcgcgt tgggtgaggt gcagtcggaa
    14881 gtcagctctc cttcggatga ggaggacttt gtggtcttca cgactggtgt gaacatttct
    14941 tccaagggag ataagcttgg tcagcagtac cagacgcccg gatcggctat cggccggggt
    15001 gctgacttca ttatcgcggg tcgcggtatc tacgccgcgc cggatcgatc cggtgcaggc
    15061 tgcgcaacag tatcagaagg aggggtggga agcctacctg gcccgtgtcg gcggaaacta
    15121 atactataaa aggaggatcg aagttctgat ggttatgaat gatatagaaa tgcaacttgc
    15181 cgcaacggat acggaagcgg aaacggacca atgtcgagca cgggtagtca gactgcggca
    15241 tcggatgtcc aaacggtatt gatcctgcag gctactatgg tgtggcacaa ggatcaatgc
    15301 ggtacgacga tttgatgcag ataagcaggc tgcgaagtag taactcttgc gtagagaaaa
    15361 tggcgacggg tggctgataa gggcggtgat aagcttaatt gtcatcgcag ataagcactg
    15421 ctgtcttgca tccaagtcag cgtcagcaga aatacgggac ttccgaaagt atatggcaaa
    15481 attaaagaac ttgactctcc agcaatgttt tgccctgacc gtcgctaaaa cgttactacc
    15541 cctatacccg tctgtttgtc ccagcccgag gcattaggtc tgactgacag cacggcgcca
    15601 tgcgggcttg ggacgccatg tccgtcgcgt gataagggtt gatccatgca gctactatcc
    15661 ttccatcgtt ccattcccat ccttgtccta tctccatcct tgaaacttta ctagtttagt
    15721 tggatgctcg agcttgctct cggctactcc gtcggcgcgc cttgcggccg ccaccgcggt
    15781 ggagctccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
    15841 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
    15901 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    15961 gcctgaatgg cgaatggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
    16021 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
    16081 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
    16141 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
    16201 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
    16261 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
    16321 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
    16381 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
    16441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    16501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    16561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    16621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    16681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    16741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    16801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    16861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    16921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    16981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    17041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    17101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    17161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    17221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    17281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    17341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    17401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    17461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    17521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    17581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    17641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    17701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    17761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    17821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
    17881 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
    17941 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
    18001 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
    18061 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
    18121 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
    18181 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
    18241 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
    18301 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
    18361 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
    18421 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    18481 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    18541 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
    18601 gctcgaaatt aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgaggtc
    18661 gacggtatcg ataagcttcg gagaatatgg agcttcatcg aatcaccggc agtaagcgaa
    18721 ggagaatgtg aagccagggg tgtatagccg tcggcgaaat agcatgccat taacctaggt
    18781 acagaagtcc aattgcttcc gatctggtaa aagattcacg agatagtacc ttctccgaag
    18841 taggtagagc gagtacccgg cgcgtaagct ccctaattgg cccatccggc atctgtaggg
    18901 cgtccaaata tcgtgcctct cctgctttgc ccggtgtatg aaaccggaaa ggccgctcag
    18961 gagctggcca gcggcgcaga ccgggaacac aagctggcag tcgacccatc cggtgctctg
    19021 cactcgacct gctgaggtcc ctcagtccct ggtaggcagc tttgccccgt ctgtccgccc
    19081 ggtgtgtcgg cggggttgac aaggtcgttg cgtcagtcca acatttgttg ccatattttc
    19141 ctgctctccc caccagctgc tcttttcttt tctctttctt ttcccatctt cagtatattc
    19201 atcttcccat ccaagaacct ttatttcccc taagtaagta ctttgctaca tccatactcc
    19261 atccttccca tcccttattc ctttgaacct ttcagttcga gctttcccac ttcatcgcag
    19321 cttgactaac agctaccccg cttgagcaga catcaccgaa ttcaccatgt ctagactgga
    19381 caagagcaaa gtcataaacg gcgctctgga attactcaat ggagtcggta tcgaaggcct
    19441 gacgacaagg aaactcgctc aaaagctggg agttgagcag cctaccctgt actggcacgt
    19501 gaagaacaag cgggccctgc tcgatgccct gccaatcgag atgctggaca ggcatcatac
    19561 ccacttctgc cccctggaag gcgagtcatg gcaagacttt ctgcggaaca acgccaagtc
    19621 attccgctgt gctctcctct cacatcgcga cggggctaaa gtgcatctcg gcacccgccc
    19681 aacagagaaa cagtacgaaa ccctggaaaa tcagctcgcg ttcctgtgtc agcaaggctt
    19741 ctccctggag aacgcactgt acgctctgtc cgccgtgggc cactttacac tgggctgcgt
    19801 attggaggaa caggagcatc aagtagcaaa agaggaaaga gagacaccta ccaccgattc
    19861 tatgccccca cttctgagac aagcaattga gctgttcgac cggcagggag ccgaacctgc
    19921 cttccttttc ggcctggaac taatcatatg tggcctggag aaacagctaa agtgcgaaag
    19981 cggcgggccg gccgacgccc ttgacgattt tgacttagac atgctcccag ccgatgccct
    20041 tgacgacttt gaccttgata tgctgcctgc tgacgctctt gacgattttg accttgacat
    20101 gctccccggg taactaagta aggatccact agtacagcag aagaatctct ctccgctgtt
    20161 gcttcagtgt ctgccatgca ttaacttcat cctactgtcc tacccgcagt acccattcac
    20221 atttgcggca gataccaggt tgttttctat tccctcggtt cttcagttct tcagatatta
    20281 cattactcga gaaattggcg gcgatgcagg agtttggttg gttttaattg tttcagtctc
    20341 cttgacctga tttgtatgac atgcaatgtt tcgacggaag actatctcgg tgaatatacg
    20401 tcatgaatca tgcggccgcg cgtatcacga ggccctttcg tcttcactcg agtttaccac
//
