LOCUS       sequence-20543-e         640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG414GPD-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(119..243)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(320..341)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(581..600)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cggggaagaa gtggctgatt ctcagccacc gcgaatgaca tcaaaaacgc cattaacctg
       61 atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca
      121 tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta
      181 atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt
      241 ggtgatgggc tgcaggaatt cgatatcaag cttaatggtg agcaagggcg aggagctgtt
      301 caccggggtg gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
      361 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
      421 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
      481 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
      541 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
      601 ccgcgcccaa gtgaagttcg agggcgacac cctggtgaac
//
