LOCUS       sequence-20545-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GAL-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(49..173)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(181..197)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(181..197)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(212..653)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(218..241)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    536..653
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(677..697)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(677..695)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 gaggccggaa gctaaagtgt aaagcctggg ggtgcctaat gcggccgcca tagtgactgg
       61 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata
      121 ttgatattta tatcatttta cgtttctcgt tcagcttttt tgtacaaact tgtgatgggg
      181 gatccactag ttctagaatc cggggttttt tctccttgac gttaaagtat agaggtatat
      241 taacaatttt ttgttgatac ttttattaca tttgaataag aagtaataca aaccgaaaat
      301 gttgaaagta ttagttaaag tggttatgca gtttttgcat ttatatatct gttaatagat
      361 caaaaatcat cgcttcgctg attaattacc ccagaaataa ggctaaaaaa ctaatcgcat
      421 tatcatccta tggttgttaa tttgattcgt tcatttgaag gtttgtgggg ccaggttact
      481 gccaattttt cctcttcata accataaaag ctagtattgt agaatcttta ttgttcggag
      541 cagtgcggcg cgaggcacat ctgcgtttca ggaacgcgac cggtgaagac gaggacgcac
      601 ggaggagagt cttccttcgg agggctgtca cccgctcggc ggcttctaat ccgtactaga
      661 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatgg
//
