LOCUS       sequence-205451-       10917 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express PKA-SPARK, an activity reporter of protein kinase A in 
            Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Zhang Q, Huang H, Lu Q, Wu R, Chung CI, Zhang SQ, Torra J, Schepis 
            A, Coughlin SR, Kornberg TB, Shu X
  TITLE     Visualizing Dynamics of Cell Signaling In Vivo with a Phase 
            Separation-Based Kinase Reporter.
  JOURNAL   Mol Cell. 2018 Jan 3. pii: S1097-2765(17)30936-X. doi: 
            10.1016/j.molcel.2017.12.008.
  PUBMED    29307513
REFERENCE   2  (bases 1 to 10917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Jan 3. pii: S1097-2765(17)30936-X. doi: 
            10.1016/j.molcel.2017.12.008."
COMMENT     Sequence Label: pUASattB-PKA-SPARK
FEATURES             Location/Qualifiers
     source          1..10917
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             673..726
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1210..1212,1213..1215,1216..1926)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     intron          2229..2294
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             2424..2444
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2716..2850
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2753..2772)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2807..2826
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    2879..2948
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(3167..3185)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3206..3222)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3206..3222)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3219..3241)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3230..3246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3254..3260,3261..3278,3279..3284))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3299..3320
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3437..3454)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3608..4196)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3688..3707)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4367..5158,5159..5227))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4990..5009
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5228..5332)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5358..5813)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5495..5516)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5707..5726
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5940..5962
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5954..5971
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     5955..5971
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5978..5997
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5978..5996
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6030..6048)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(6500..6571,6973..7246,7321..7975,8037..8352,8556..8687,
                     8758..9372)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    10147..10180
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    10213..10307
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        10326..10564
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     10511..10532
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     CDS             join(10678..10680,10681..10683,10684..10917,1..477)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
ORIGIN
        1 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
       61 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      121 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      181 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      241 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      301 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      361 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      421 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaaggga
      481 tccggcagtg ctggtggtag tgctggtggt agtgctggtg gtagtgctgg tggcagtgct
      541 ggtggtagtg ctggtggtag tgctggtggc tctagaggag aaatcgcaaa gagtctgaaa
      601 gaaattgcaa aatccttgaa ggaaatagcg tggtcactga aagaaattgc caagtccctc
      661 aaagggggta ccgagggcag aggaagtctg ctaacatgcg gtgacgtcga ggagaatcct
      721 ggcccaaagc ttgccaccat gcataagttt tctcaagaac agatcggcga aaacattgtg
      781 tgcagggtca tttgtaccac gggtcaaatt cccatccgag atttgtcagc tgatatttca
      841 caagtgctta aggaaaaacg atccataaag aaagtttgga catttggtag aaacccagcc
      901 tgtgactatc atttaggaaa catttcaaga ctgtcaaata agcatttcca aatactacta
      961 ggagaagacg gtaacctttt attgaatgac atttccacta atgggacctg gttaaatggg
     1021 caaaaagtcg agaagaacag caatcagtta ctgtctcaag gtgatgaaat aaccgttggt
     1081 gtaggcgtgg aatcagatat tttatctctg gtcattttca taaacgacaa atttaagcag
     1141 tgcctcgaac agaacaaagt tgatcgcctc gagtccggac tcagatctgg cagcggtgga
     1201 ggcgatatca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     1261 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     1321 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     1381 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     1441 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     1501 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     1561 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     1621 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     1681 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     1741 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     1801 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     1861 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     1921 tacaagggat ccggcagtgc tggtggtagt gctggtggta gtgctggtgg tagtgctggt
     1981 ggcagtgctg gtggtagtgc tggtggtagt gctggtggct ctagaacctt gagagaaatc
     2041 gaggaattgt tgaggaaaat cattgaagac agcgtgcgca gtgtggccga gctggaggac
     2101 attgagaagt ggctgaagaa aatctaagaa ttcgggcccg tttaatctag aggatctttg
     2161 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     2221 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     2281 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     2341 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     2401 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     2461 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     2521 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     2581 aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag
     2641 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     2701 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     2761 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     2821 ctcatcaatg tatcttatca tgtctggatc cactagtgtc gacgatgtag gtcacggtct
     2881 cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg tactccacct
     2941 cacccatctg gtccatcatg atgaacgggt cgaggtggcg gtagttgatc ccggcgaacg
     3001 cgcggcgcac cgggaagccc tcgccctcga aaccgctggg cgcggtggtc acggtgagca
     3061 cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc gggttctcga
     3121 cggtcacggc gggcatgtcg acactagttc tagccagctt ttgttccctt tagtgagggt
     3181 taatttcgag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     3241 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     3301 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     3361 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     3421 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     3481 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     3541 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     3601 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     3661 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     3721 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     3781 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     3841 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     3901 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     3961 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     4021 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     4081 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     4141 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     4201 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     4261 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     4321 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     4381 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     4441 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     4501 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     4561 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     4621 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     4681 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     4741 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     4801 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     4861 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     4921 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     4981 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     5041 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     5101 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     5161 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     5221 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     5281 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     5341 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     5401 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     5461 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     5521 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     5581 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     5641 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     5701 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     5761 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
     5821 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     5881 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     5941 ccagtcacga cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga
     6001 attgggtacg taccgggccc ctagtatgta tgtaagttaa taaaacccat ttttgcggaa
     6061 agtagataaa aaaaacattt ttttttttta ctgcactgga tatcattgaa cttatctgat
     6121 cagttttaaa tttacttcga tccaagggta tttgatgtac caggttcttt cgattacctc
     6181 tcactcaaaa tgacattcca ctcaaagtca gcgctgtttg cctccttctc tgtccacaga
     6241 aatatcgccg tctctttcgc cgctgcgtcc gctatctctt tcgccaccgt ttgtagcgtt
     6301 acgtagcgtc aatgtccgcc ttcagttgca ttttgtcagc ggtttcgtga cgaagctcca
     6361 agcggtttac gccatcaatt aaacacaaag tgctgtgcca aaactcctct cgcttcttat
     6421 ttttgtttgt tttttgagtg attggggtgg tgattggttt tgggtgggta agcaggggaa
     6481 agtgtgaaaa atcccggcaa tgggccaaga ggatcaggag ctattaattc gcggaggcag
     6541 caaacaccca tctgccgagc atctgaacaa tgtgagtagt acatgtgcat acatcttaag
     6601 ttcacttgat ctataggaac tgcgattgca acatcaaatt gtctgcggcg tgagaactgc
     6661 gacccacaaa aatcccaaac cgcaattgca caaacaaata gtgacacgaa acagattatt
     6721 ctggtagctg ttctcgctat ataagacaat ttttgagatc atatcatgat caagacatct
     6781 aaaggcattc attttcgact atattctttt ttacaaaaaa tataacaacc agatatttta
     6841 agctgatcct agatgcacaa aaaataaata aaagtataaa cctacttcgt aggatacttc
     6901 ggggtacttt ttgttcgggg ttagatgagc ataacgcttg tagttgatat ttgagatccc
     6961 ctatcattgc agggtgacag cggagcggct tcgcagagct gcattaacca gggcttcggg
     7021 caggccaaaa actacggcac gctccggcca cccagtccgc cggaggactc cggttcaggg
     7081 agcggccaac tagccgagaa cctcacctat gcctggcaca atatggacat ctttggggcg
     7141 gtcaatcagc cgggctccgg atggcggcag ctggtcaacc ggacacgcgg actattctgc
     7201 aacgagcgac acataccggc gcccaggaaa catttgctca agaacggtga gtttctattc
     7261 gcagtcggct gatctgtgtg aaatcttaat aaagggtcca attaccaatt tgaaactcag
     7321 tttgcggcgt ggcctatccg ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa
     7381 agacgaccct gctgaatgcc cttgcctttc gatcgccgca gggcatccaa gtatcgccat
     7441 ccgggatgcg actgctcaat ggccaacctg tggacgccaa ggagatgcag gccaggtgcg
     7501 cctatgtcca gcaggatgac ctctttatcg gctccctaac ggccagggaa cacctgattt
     7561 tccaagccat ggtgcggatg ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg
     7621 atcaggtgat ccaggagctt tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg
     7681 gcagggtgaa aggtctgtcc ggcggagaaa ggaagcgtct ggcattcgcc tccgaggctc
     7741 taaccgatcc gccgcttctg atctgcgatg agcccacctc cggactggac tcctttaccg
     7801 cccacagcgt cgtccaggtg ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga
     7861 ccattcatca gccgtcttcc gagctgtttg agctctttga caagatcctt ctgatggccg
     7921 agggcagggt agctttcttg ggcactccca gcgaagccgt cgacttcttt tcctagtgag
     7981 ttcgatgtgt ttattaaggg tatctagtat tacataacat ctcaactcct atccagcgtg
     8041 ggtgcccagt gtcctaccaa ctacaatccg gcggactttt acgtacaggt gttggccgtt
     8101 gtgcccggac gggagatcga gtcccgtgat cggatcgcca agatatgcga caattttgcc
     8161 attagcaaag tagcccggga tatggagcag ttgttggcca ccaaaaatct ggagaagcca
     8221 ctggagcagc cggagaatgg gtacacctac aaggccacct ggttcatgca gttccgggcg
     8281 gtcctgtggc gatcctggct gtcggtgctc aaggaaccac tcctcgtaaa agtgcgactt
     8341 attcagacaa cggtgagtgg ttccagtgga aacaaatgat ataacgctta caattcttgg
     8401 aaacaaattc gctagatttt agatagaatt gcctgattcc acacccttct tagttttttt
     8461 caatgagatg tatagtttat agttttgcag aagataaata aatttcattt aactcgcgaa
     8521 tattaatgag atgcgagtaa cattttaatt tgcagatggt tgccatcttg attggcctca
     8581 tctttttggg ccaacaactc acgcaagtgg gtgtgatgaa tatcaacgga gccatcttcc
     8641 tcttcctgac caacatgacc tttcaaaacg tctttgccac gataaatgta agtcatgttt
     8701 agaatacatt tgcatttcaa taatttacta actttctaat gaatcgattc gatttaggtg
     8761 ttcacctcag agctgccagt ttttatgagg gaggcccgaa gtcgacttta tcgctgtgac
     8821 acatactttc tgggcaaaac gattgccgaa ttgccgcttt ttctcacagt gccactggtc
     8881 ttcacggcga ttgcctatcc gatgatcgga ctgcgggccg gagtgctgca cttcttcaac
     8941 tgcctggcgc tggtcactct ggtggccaat gtgtcaacgt ccttcggata tctaatatcc
     9001 tgcgccagct cctcgacctc gatggcgctg tctgtgggtc cgccggttat cataccattc
     9061 ctgctctttg gcggcttctt cttgaactcg ggctcggtgc cagtatacct caaatggttg
     9121 tcgtacctct catggttccg ttacgccaac gagggtctgc tgattaacca atgggcggac
     9181 gtggagccgg gcgaaattag ctgcacatcg tcgaacacca cgtgccccag ttcgggcaag
     9241 gtcatcctgg agacgcttaa cttctccgcc gccgatctgc cgctggacta cgtgggtctg
     9301 gccattctca tcgtgagctt ccgggtgctc gcatatctgg ctctaagact tcgggcccga
     9361 cgcaaggagt agccgacata tatccgaaat aactgcttgt tttttttttt taccattatt
     9421 accatcgtgt ttactgttta ttgccccctc aaaaagctaa tgtaattata tttgtgccaa
     9481 taaaaacaag atatgaccta tagaatacaa gtatttcccc ttcgaacatc cccacaagta
     9541 gactttggat ttgtcttcta accaaaagac ttacacacct gcatacctta catcaaaaac
     9601 tcgtttatcg ctacataaaa caccgggata tattttttat atacatactt ttcaaatcgc
     9661 gcgccctctt cataattcac ctccaccaca ccacgtttcg tagttgctct ttcgctgtct
     9721 cccacccgct ctccgcaaca cattcacctt ttgttcgacg accttggagc gactgtcgtt
     9781 agttccgcgc gattcggttc gctcaaatgg ttccgagtgg ttcatttcgt ctcaatagaa
     9841 attagtaata aatatttgta tgtacaattt atttgctcca atatatttgt atatatttcc
     9901 ctcacagcta tatttattct aatttaatat tatgactttt taaggtaatt ttttgtgacc
     9961 tgttcggagt gattagcgtt acaatttgaa ctgaaagtga catccagtgt ttgttccttg
    10021 tgtagatgca tctcaaaaaa atggtgggca taatagtgtt gtttatatat atcaaaaata
    10081 acaactataa taataagaat acatttaatt tagaaaatgc ttggatttca ctggaactag
    10141 gctagcataa cttcgtataa tgtatgctat acgaagttat gctagcggat ccaagcttgc
    10201 atgcctgcag gtcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg
    10261 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agactctagc
    10321 gagcgccgga gtataaatag aggcgcttcg tctacggagc gacaattcaa ttcaaacaag
    10381 caaagtgaac acgtcgctaa gcgaaagcta agcaaataaa caagcgcagc tgaacaagct
    10441 aaacaatctg cagtaaagtg caagttaaag tgaatcaatt aaaagtaacc agcaaccaag
    10501 taaatcaact gcaactactg aaatctgcca agaagtaatt attgaataca agaagagaac
    10561 tctgaatagg gaattgggaa ttccaaaatg ggcggtggag ggagcctgcg tcgcgccacc
    10621 ctggtggacg gcggactcga gtccggactc agatctggca gcggtggagg cgatatcatg
    10681 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
    10741 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
    10801 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
    10861 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaag
//
