LOCUS       sequence-205456-       12410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express ERK-SPARK, an activity reporter of ERK in Drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12410)
  AUTHORS   Zhang Q, Huang H, Lu Q, Wu R, Chung CI, Zhang SQ, Torra J, Schepis 
            A, Coughlin SR, Kornberg TB, Shu X
  TITLE     Visualizing Dynamics of Cell Signaling In Vivo with a Phase 
            Separation-Based Kinase Reporter.
  JOURNAL   Mol Cell. 2018 Jan 3. pii: S1097-2765(17)30936-X. doi: 
            10.1016/j.molcel.2017.12.008.
  PUBMED    29307513
REFERENCE   2  (bases 1 to 12410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2018 Jan 3. pii: S1097-2765(17)30936-X. doi: 
            10.1016/j.molcel.2017.12.008."
COMMENT     Sequence Label: pUASattB-ERK-SPARK
FEATURES             Location/Qualifiers
     source          1..12410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(387..389,390..392,393..1103)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             1299..1352
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1359..1368
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1569..1571,1572..1574,1575..2285)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    join(3951..4152,4153..4183)
                     /label=P element 3' end
                     /note="P element 3' end"
     primer_bind     complement(4721..4740)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4840..4862
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4900..4918)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    5189..5258
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        5367..5471
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5472..5540,5541..6332)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5690..5709)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6503..7091
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6992..7011
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    join(7326..7356,7357..7911)
                     /label=P element 5' end
     CDS             complement(join(8690..9304,9375..9506,9710..10025,
                     10087..10741,10816..11089,11491..11562))
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     primer_bind     12014..12032
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
ORIGIN
        1 gagaggaaag gttgtgtgcg gacgaatttt tttttgaaaa ccggtgatag agcctgaacc
       61 agaaaagata aaagaaggct ataccagtgg gagtacacaa acagagtaag tttgaatagt
      121 aaaaaaaatc atttatgtaa acaataacgt gactgtgcgt taggtcctgt tcattggtac
      181 ccgcccgggg atcagatccg cggccgcata ggccactagt ggatctggat cccaaaatgg
      241 ctgaccaact gactgaagag tggtcgacgg taccgcggac cggtcctgac gtaccacgta
      301 caccagtagg aaaggcaaag ctgtcattcc aattcccggg cggactcgag tccggactca
      361 gatctggcag cggtggaggc gatatcatgg tgagcaaggg cgaggagctg ttcaccgggg
      421 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      481 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
      541 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      601 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      661 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      721 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      781 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
      841 atatcatggc cgacaagcag aagaacggca tcaaggccaa cttcaagatc cgccacaaca
      901 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      961 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     1021 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     1081 tcggcatgga cgagctgtac aagggatccg gcagtgctgg tggtagtgct ggtggtagtg
     1141 ctggtggtag tgctggtggc agtgctggtg gtagtgctgg tggtagtgct ggtggctcta
     1201 gaggagaaat cgcaaagagt ctgaaagaaa ttgcaaaatc cttgaaggaa atagcgtggt
     1261 cactgaaaga aattgccaag tccctcaaag ggggtaccga gggcagagga agtctgctaa
     1321 catgcggtga cgtcgaggag aatcctggcc caaagcttgc caccatggcg gacgaggaga
     1381 agctgccgcc cggctgggag aagcgcatga gccgcagctc aggccgagtg tactacttca
     1441 accacatcac taacgccagc cagtgggagc ggcccagcgg caacagcagc agtggtggca
     1501 aaaacgggca gggggagcct gccaggctcg agtccggact cagatctggc agcggtggag
     1561 gcgatatcat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     1621 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     1681 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     1741 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc
     1801 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     1861 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     1921 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     1981 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     2041 agaagaacgg catcaaggcc aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     2101 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     2161 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     2221 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     2281 acaagggatc cggcagtgct ggtggtagtg ctggtggtag tgctggtggt agtgctggtg
     2341 gcagtgctgg tggtagtgct ggtggtagtg ctggtggctc tagaaccttg agagaaatcg
     2401 aggaattgtt gaggaaaatc attgaagaca gcgtgcgcag tgtggccgag ctggaggaca
     2461 ttgagaagtg gctgaagaaa atctaagaat tcgggcccgt ttaatctaga gtcgacctcg
     2521 aacgttaacg ttaacgtaac gttaactcga ggagcttgat aacattatac ctaaacccat
     2581 ggtcaagagt aaacatttct gcctttgaag ttgagaacac aattaagcat cccctggtta
     2641 aacctgacat tcatacttgt taatagcgcc ataaacatag caccaatttc gaagaaatca
     2701 gttaaaagca attagcaatt agcaattagc aataactctg ctgacttcaa aacgagaaga
     2761 gttgcaagta tttgtaaggc acagtttata gaccaccgac ggctcattag ggctcgtcat
     2821 gtaactaagc gcggtgaaac ccaattgaac atatagtgga attattatta tcaatgggga
     2881 agatttaacc ctcaggtagc aaagtaattt aattgcaaat agagagtcct aagactaaat
     2941 aatatattta aaaatctggc cctttgacct tgcttgtcag gtgcatttgg gttcaatcgt
     3001 aagttgcttc tatataaaca ctttccccat ccccgcaata atgaagaata ccgcagaata
     3061 aagagagatt tgcaacaaaa aataaaggca ttgcgaaaac tttttatggg ggatcattac
     3121 actcgggcct acggttacaa ttcccagcca cttaagcgac aagtttggcc aacaatccat
     3181 ctaatagcta atagcgcaat cactggtaat cgcaagagta tataggcaat agaacccatg
     3241 gatttgacca aaggtaaccg agacaatgga gaagcaagag gatttcaaac tgaacaccca
     3301 cagtgctgtg tactaccact ggcgcgtttg ggagctcact ggcctgatgc gtcctccggg
     3361 cgtttcaagc ctgctttacg tggtatactc cattacggtc aacttggtgg tcaccgtgct
     3421 gtttcccttg agcttgctgg ccaggctgct gttcaccacc aacatggccg gattgtgcga
     3481 gaacctgacc ataactatta ccgatattgt ggccaatttg aagtttgcga atgtgtacat
     3541 ggtgaggaag cagctccatg agattcgctc tctcctaagg ctcatggacg ctagagcccg
     3601 gctggtgggc gatcccgagg agatttctgc cttgaggaag gaagtgaata tcgcacaggg
     3661 cactttccgc acctttgcca gtattttcgt atttggcact actttgagtt gcgtccgcgt
     3721 ggtcgttcgc ccggatcgag agctcctgta tccggcctgg ttcggcgttg actggatgca
     3781 ctccaccaga aactatgtgc tcatcaatat ctaccagctc ttcggcttga tagtgcaggc
     3841 tatacagaac tgcgctagtg actcctatcc gcctgcgttt ctctgcctgc tcacgggtca
     3901 tatgcgtgct ttggagctga gggtgcggcg gattggctgc agccaagctt tgcgtactcg
     3961 caaattatta aaaataaaac tttaaaaata atttcgtcta attaatatta tgagttaatt
     4021 caaaccccac ggacatgcta agggttaatc aacaatcata tcgctgtctc actcagactc
     4081 aatacgacac tcagaatact attcctttca ctcgcactta ttgcaagcat acgttaagtg
     4141 gatgtctctt gccgacggga ccaccttatg ttatttcatc atggtctggc cattctcatc
     4201 gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg caaggagtag
     4261 ccgacatata tccgaaataa ctgcttgttt ttttttttta ccattattac catcgtgttt
     4321 actgtttatt gccccctcaa aaagctaatg taattatatt tgtgccaata aaaacaagat
     4381 atgacctata gaatacaagt atttcccctt cgaacatccc cacaagtaga ctttggattt
     4441 gtcttctaac caaaagactt acacacctgc ataccttaca tcaaaaactc gtttatcgct
     4501 acataaaaca ccgggatata ttttttatat acatactttt caaatcgcgc gccctcttca
     4561 taattcacct ccaccacacc acgtttcgta gttgctcttt cgctgtctcc cacccgctct
     4621 ccgcaacaca ttcacctttt gttcgacgac cttggagcga ctgtcgttag ttccgcgcga
     4681 ttcggtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg
     4741 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     4801 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     4861 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     4921 tataggttaa tgtcatgata ataatggttt cttagacgtc gcgaattagg ccttctagtg
     4981 gatcaattcg gcttgtcgac atgcccgccg tgaccgtcga gaacccgctg acgctgcccc
     5041 gcgtatccgc acccgccgac gccgtcgcac gtcccgtgct caccgtgacc accgcgccca
     5101 gcggtttcga gggcgagggc ttcccggtgc gccgcgcgtt cgccgggatc aactaccgcc
     5161 acctcgaccc gttcatcatg atggaccaga tgggtgaggt ggagtacgcg cccggggagc
     5221 ccaagggcac gccctggcac ccgcaccgcg gcttcgagac cgtgacctac atcgtcgacg
     5281 gtacctgaag ccgaattgat ccggagagct cccaacgcgt tggatgcagc actaggacgt
     5341 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5401 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5461 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5521 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5581 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5641 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5701 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5761 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5821 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5881 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5941 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     6001 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     6061 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     6121 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6181 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6241 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6301 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6361 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6421 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6481 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6541 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6601 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6661 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6721 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6781 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6841 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6901 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6961 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     7021 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     7081 gcctatggaa aaacgccttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     7141 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     7201 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     7261 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     7321 tggcccatga tgaaataaca taaggtggtc ccgtcgatag ccgaagctta ccgaagtata
     7381 cacttaaatt cagtgcacgt ttgcttgttg agaggaaagg ttgtgtgcgg acgaattttt
     7441 ttttgaaaac attaaccctt acgtggaata aaaaaaaatg aaatattgca aattttgctg
     7501 caaagctgtg actggagtaa aattaattca cgtgccgaag tgtgctatta agagaaaatt
     7561 gtgggagcag agccttgggt gcagccttgg tgaaaactcc caaatttgtg atacccactt
     7621 taatgattcg cagtggaagg ctgcacctgc aaaaggtcag acatttaaaa ggaggcgact
     7681 caacgcagat gccgtaccta gtaaagtgat agagcctgaa ccagaaaaga taaaagaagg
     7741 ctataccagt gggagtacac aaacagagta agtttgaata gtaaaaaaaa tcatttatgt
     7801 aaacaataac gtgactgtgc gttaggtcct gttcattgtt taatgaaaat aagagcttga
     7861 gggaaaaaat tcgtactttg gagtacgaaa tgcgtcgttt agagcagcag ccgaattaat
     7921 tctagttcca gtgaaatcca agcattttct aaattaaatg tattcttatt attatagttg
     7981 ttatttttga tatatataaa caacactatt atgcccacca tttttttgag atgcatctac
     8041 acaaggaaca aacactggat gtcactttca gttcaaattg taacgctaat cactccgaac
     8101 aggtcacaaa aaattacctt aaaaagtcat aatattaaat tagaataaat atagctgtga
     8161 gggaaatata tacaaatata ttggagcaaa taaattgtac atacaaatat ttattactaa
     8221 tttctattga gacgaaatga accactcgga accatttgag cgaaccgaat cgcgcggaac
     8281 taacgacagt cgctccaagg tcgtcgaaca aaaggtgaat gtgttgcgga gagcgggtgg
     8341 gagacagcga aagagcaact acgaaacgtg gtgtggtgga ggtgaattat gaagagggcg
     8401 cgcgatttga aaagtatgta tataaaaaat atatcccggt gttttatgta gcgataaacg
     8461 agtttttgat gtaaggtatg caggtgtgta agtcttttgg ttagaagaca aatccaaagt
     8521 ctacttgtgg ggatgttcga aggggaaata cttgtattct ataggtcata tcttgttttt
     8581 attggcacaa atataattac attagctttt tgagggggca ataaacagta aacacgatgg
     8641 taataatggt aaaaaaaaaa aacaagcagt tatttcggat atatgtcggc tactccttgc
     8701 gtcgggcccg aagtcttaga gccagatatg cgagcacccg gaagctcacg atgagaatgg
     8761 ccagacccac gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga
     8821 ccttgcccga actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca
     8881 cgtccgccca ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg
     8941 acaaccattt gaggtatact ggcaccgagc ccgagttcaa gaagaagccg ccaaagagca
     9001 ggaatggtat gataaccggc ggacccacag acagcgccat cgaggtcgag gagctggcgc
     9061 aggatattag atatccgaag gacgttgaca cattggccac cagagtgacc agcgccaggc
     9121 agttgaagaa gtgcagcact ccggcccgca gtccgatcat cggataggca atcgccgtga
     9181 agaccagtgg cactgtgaga aaaagcggca attcggcaat cgttttgccc agaaagtatg
     9241 tgtcacagcg ataaagtcga cttcgggcct ccctcataaa aactggcagc tctgaggtga
     9301 acacctaaat cgaatcgatt cattagaaag ttagtaaatt attgaaatgc aaatgtattc
     9361 taaacatgac ttacatttat cgtggcaaag acgttttgaa aggtcatgtt ggtcaggaag
     9421 aggaagatgg ctccgttgat attcatcaca cccacttgcg tgagttgttg gcccaaaaag
     9481 atgaggccaa tcaagatggc aaccatctgc aaattaaaat gttactcgca tctcattaat
     9541 attcgcgagt taaatgaaat ttatttatct tctgcaaaac tataaactat acatctcatt
     9601 gaaaaaaact aagaagggtg tggaatcagg caattctatc taaaatctag cgaatttgtt
     9661 tccaagaatt gtaagcgtta tatcatttgt ttccactgga accactcacc gttgtctgaa
     9721 taagtcgcac ttttacgagg agtggttcct tgagcaccga cagccaggat cgccacagga
     9781 ccgcccggaa ctgcatgaac caggtggcct tgtaggtgta cccattctcc ggctgctcca
     9841 gtggcttctc cagatttttg gtggccaaca actgctccat atcccgggct actttgctaa
     9901 tggcaaaatt gtcgcatatc ttggcgatcc gatcacggga ctcgatctcc cgtccgggca
     9961 caacggccaa cacctgtacg taaaagtccg ccggattgta gttggtagga cactgggcac
    10021 ccacgctgga taggagttga gatgttatgt aatactagat acccttaata aacacatcga
    10081 actcactagg aaaagaagtc gacggcttcg ctgggagtgc ccaagaaagc taccctgccc
    10141 tcggccatca gaaggatctt gtcaaagagc tcaaacagct cggaagacgg ctgatgaatg
    10201 gtcaggatga cggtcttgcc cttctgcgac agcttcttca gcacctggac gacgctgtgg
    10261 gcggtaaagg agtccagtcc ggaggtgggc tcatcgcaga tcagaagcgg cggatcggtt
    10321 agagcctcgg aggcgaatgc cagacgcttc ctttctccgc cggacagacc tttcaccctg
    10381 ccgggcacac cgatgatcgt gtgctgacat ttgctgagcg aaagctcctg gatcacctga
    10441 tccacgcggg ccactcgctg ccgataggtc agatgtcgtg gcatccgcac catggcttgg
    10501 aaaatcaggt gttccctggc cgttagggag ccgataaaga ggtcatcctg ctggacatag
    10561 gcgcacctgg cctgcatctc cttggcgtcc acaggttggc cattgagcag tcgcatcccg
    10621 gatggcgata cttggatgcc ctgcggcgat cgaaaggcaa gggcattcag cagggtcgtc
    10681 tttccggcac cggaactgcc catcacggcc aaaagttcgc ccggataggc cacgccgcaa
    10741 actgagtttc aaattggtaa ttggaccctt tattaagatt tcacacagat cagccgactg
    10801 cgaatagaaa ctcaccgttc ttgagcaaat gtttcctggg cgccggtatg tgtcgctcgt
    10861 tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga
    10921 ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc
    10981 tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct
    11041 gcccgaagcc ctggttaatg cagctctgcg aagccgctcc gctgtcaccc tgcaatgata
    11101 ggggatctca aatatcaact acaagcgtta tgctcatcta accccgaaca aaaagtaccc
    11161 cgaagtatcc tacgaagtag gtttatactt ttatttattt tttgtgcatc taggatcagc
    11221 ttaaaatatc tggttgttat attttttgta aaaaagaata tagtcgaaaa tgaatgcctt
    11281 tagatgtctt gatcatgata tgatctcaaa aattgtctta tatagcgaga acagctacca
    11341 gaataatctg tttcgtgtca ctatttgttt gtgcaattgc ggtttgggat ttttgtgggt
    11401 cgcagttctc acgccgcaga caatttgatg ttgcaatcgc agttcctata gatcaagtga
    11461 acttaagatg tatgcacatg tactactcac attgttcaga tgctcggcag atgggtgttt
    11521 gctgcctccg cgaattaata gctcctgatc ctcttggccc attgccggga tttttcacac
    11581 tttcccctgc ttacccaccc aaaaccaatc accaccccaa tcactcaaaa aacaaacaaa
    11641 aataagaagc gagaggagtt ttggcacagc actttgtgtt taattgatgg cgtaaaccgc
    11701 ttggagcttc gtcacgaaac cgctgacaaa atgcaactga aggcggacat tgacgctacg
    11761 taacgctaca aacggtggcg aaagagatag cggacgcagc ggcgaaagag acggcgatat
    11821 ttctgtggac agagaaggag gcaaacagcg ctgactttga gtggaatgtc attttgagtg
    11881 agaggtaatc gaaagaacct ggtacatcaa atacccttgg atcgaagtaa atttaaaact
    11941 gatcagataa gttcaatgat atccagtgca gtaaaaaaaa aaaatgtttt ttttatctac
    12001 tttccgcaaa aatgggtttt attaacttac atacatacta gaattggccg ctctagcccc
    12061 ccctcgaatg ttctctctct tctcttctct ctctctttct cgaatgttct ctctcttctc
    12121 ttctctctct ctttctcgag gtcatcaagc ttaggcctcc aaggcggagt actgtcctcc
    12181 gggctggcgg agtactgtcc tccggcaagg cggagtactg tcctccgggc tggcggagta
    12241 ctgtcctccg gcaaggcgga gtactgtcct ccgggctggc ggagtactgt cctccggcaa
    12301 ggcggagtac tgtcctccgg gctggcggag tactgtcctc cggcaagggt cgagtcgata
    12361 gccgaagctt accgaagtat acacttaaat tcagtgcacg tttgcttgtt
//
