LOCUS       sequence-205459-        1473 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  destabilizing domains for yeast (S. cerevisiae) derived from the 
            human estrogen receptor ligand binding domain. This ERdd can be 
            stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1473)
  TITLE     pScDD2_ERdd
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pScDD2_ERdd
FEATURES             Location/Qualifiers
     source          1..1473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..714
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             1..714
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 atgtctaaag gtgaagaatt attcactggt gttgtcccaa ttttggttga attagatggt
       61 gatgttaatg gtcacaaatt ttctgtctcc ggtgaaggtg aaggtgatgc tacttacggt
      121 aaattgacct taaaatttat ttgtactact ggtaaattgc cagttccatg gccaacctta
      181 gtcactactt taacttatgg tgttcaatgt ttttctagat acccagatca tatgaaacaa
      241 catgactttt tcaagtctgc catgccagaa ggttatgttc aagaaagaac tatttttttc
      301 aaagatgacg gtaactacaa gaccagagct gaagtcaagt ttgaaggtga taccttagtt
      361 aatagaatcg aattaaaagg tattgatttt aaagaagatg gtaacatttt aggtcacaaa
      421 ttggaataca actataactc tcacaatgtt tacatcatgg ctgacaaaca aaagaatggt
      481 atcaaagtta acttcaaaat tagacacaac attgaagatg gttctgttca attagctgac
      541 cattatcaac aaaatactcc aattggtgat ggtccagtct tgttaccaga caaccattac
      601 ttatccactc aatctgcctt atccaaagat ccaaacgaaa agagagacca catggtcttg
      661 ttagaatttg ttactgctgc tggtattacc catggtatgg atgaattgta caaaactagt
      721 ggtagaggag aaggtcaaat ggctctgtcc ttgactgctg accaaatggt ttctgctttg
      781 ttggacgctg aaccaccaat cttgtactct gaatacgacc caactagacc attctctgaa
      841 gcttctatga tgggtttgtt gactaacttg gctgacagag aattggttca catgatcaac
      901 tgggctaaga gagttccagg tttcgttgac ttgactttgc acgaccaagt tcacttgttg
      961 ggatgtgctt ggttggaaat cttgatgatc ggtttggttt ggagatctat ggaacaccca
     1021 ggtaagttgt tgttcgctcc aaacttgttg ttggacagaa accaaggtaa gtgtgttgaa
     1081 ggtatggttg aaatcttcga catgttgttg gctacttctt ctggattcag aatgatgaac
     1141 ttgcaaggtg aagaattcgt ttgtttgaag tctatcatct tgttgaactc tggtgtttac
     1201 actttcttgt cttctacttt gaagtctttg gaagaaaagg accacatcca cagagttttg
     1261 gacaagatca ctgacacttt gatccacttg atggctaagg ctggtttgac tttgcaacaa
     1321 caacaccaaa gattggctca attgttgttg atcttgtctc acatcagaca catgtctaac
     1381 aagggtatgg aacacttgta ctctatgaag tgtaagagcg ttgttccatt gtacgacttg
     1441 ttgttggaaa tgttggacgc tcacagattg taa
//
