LOCUS       sequence-205462-       11897 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11897)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 11897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-125
FEATURES             Location/Qualifiers
     source          1..11897
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..79
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     163..182
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        163..181
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    182..206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             260..277
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3825..3914
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3927..4016
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4496..5149
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             5156..5173
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(6994..7012)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7008..7055
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7092..7547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7179..7198)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7389..7410
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7639..8454)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7639..8454)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8362..8381
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8446..8465
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8576..9164
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9065..9084
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9318..9335
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9350..9489
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9415..9434)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9575..9597
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9591..9782)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(10591..11673)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     11635..11654
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(11674..11751)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
       61 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
      121 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
      181 gggaattgtg agcggataac aattcccctc tagaaataat tttgccttaa ctttaagaag
      241 gagatatacc atgggcagcc accaccacca ccaccacagc ggaatgcaga tctttgttaa
      301 gactctcacc ggaaagacaa tcaccctaga ggtggaaagc tccgacacca tcgacaacgt
      361 taaggccaag atccaggata aggagggcat tcctccggat cagcagaggc ttattttcgc
      421 cggcaagcag ctagaggatg gccgtacgtt ggctgattac aatatccaga aggaatccac
      481 cctccacttg gtcctcaggc tccgtggtgg ttaagcttgc aattaacttt aagaaggaga
      541 tatacaatgc ttcacaagcg agctagtgaa gctaacgata agaacgataa taccatcatc
      601 ggttccgatt tagcatcatc taagaaacgc cgtatcgatt tcactgaatc ttcgtctgat
      661 aagtcatctt cgattttagc ttctggtagt agcaggggtt ttcacggcga tagcgtcgtt
      721 cagcaaatcg acatggcttt tggtaattcg aaccgtcagg agattgatga agatctgcac
      781 agtcgccagc tcgccgtcta tggtcgtgag actatgaggc gtctctttgc ttccaatgtt
      841 ctcatctcgg ggatgcacgg tcttggtgcc gagattgcca agaatcttat acttgctggt
      901 gtgaagtctg tgaccttgca tgatgaaaga gttgtagagc tatgggactt atcaagcaac
      961 tttgttttct cggaggatga tgttggcaag aatagagcag atgcttctgt tcagaagtta
     1021 caggatctta acaatgctgt ggttgtctct agcttgacca aaagcttaaa caaagaggat
     1081 ctttctggct tccaggttgt tgtattctct gacataagca tggaaagagc aatcgaattt
     1141 gatgactatt gtcacagcca tcagcctcct atcgcttttg tcaaggctga tgtcaggggt
     1201 cttttcggct ctgtcttttg tgattttggg cctgaatttg cggttcttga tgttgatgga
     1261 gaggaacccc atacaggcat cattgcgtct atctctaatg agaaccaggc atttatttcc
     1321 tgtgtcgacg atgagaggct tgagtttgaa gatggtgacc tagttgtttt ctcagaagtt
     1381 gagggtatga cagaattgaa tgatgggaaa ccaaggaaga taaaaagtac acggccatat
     1441 tcattcaccc ttgatgagga cactactaac tatggaacat atgtgaaggg tggtattgtc
     1501 actcaagtga aacagccaaa gttactgaat ttcaagccat tgagggaagc gcttaaggat
     1561 ccaggggatt ttctatttag tgatttctct aagtttgatc ggcctccact ccttcactta
     1621 gcattccagg cacttgatca ttttaaagct gaagctggca gatttcctgt tgctggctca
     1681 gaagaggacg ctcagaagct tatatcgatt gccacagcca tcaataccgg gcagggtgat
     1741 ttaaaggtgg agaatgttga tcagaagctt ctaagacact tttcctttgg agccaaggct
     1801 gtcctgaatc ctatggctgc aatgtttggc ggtattgttg gacaggaggt tgttaaagct
     1861 tgctctggaa aatttcatcc tctcttccag tttttctact ttgattcagt ggagtcactc
     1921 ccctctgaac ctgtggactc gagtgacttt gcaccaagaa acagccggta tgatgcccaa
     1981 atatctgtat ttggggccaa gttccagaag aaacttgaag atgctaaagt tttcacagta
     2041 gggtctggtg ctcttggctg tgagttcttg aaaaatttgg ctctgatggg ggtttcttgt
     2101 ggaagccaag ggaagctgac agtgacagat gatgatatca ttgagaagag taacctcagt
     2161 cgtcaatttc tgttccgtga ctggaacatt ggacaggcta aatccacagt tgctgcttcg
     2221 gctgctgctg ttataaatcc tcgcttcaac attgaggccc tgcaaaaccg tgttggcgcg
     2281 gagactgaga atgtatttga cgatgccttc tgggagaact taactgttgt cgtcaatgca
     2341 ctggataatg ttaatgcgag gctctatgtt gattcgaggt gcttgtattt ccagaagcct
     2401 ctccttgagt ctggaactct tggtacaaag tgcaacacac agtcggttat cccacatcta
     2461 actgaaaatt acggtgcctc aagggaccca ccagagaaac aggcccccat gtgtacagtg
     2521 cattccttcc cgcataacat tgatcactgt ttaacttggg ctcgctctga gtttgagggt
     2581 ctgctggaga aaactcccgc tgaagtgaat gcatatcttt ctagcccagt tgagtacact
     2641 aactcaatga tgagtgctgg tgatgctcag gcgagggaca cattggagag gattgttgag
     2701 tgccttgaaa aggagaagtg tgaaaccttc caagactgct taacctgggc acgactgagg
     2761 tttgaggatt actttgtgaa ccgtgtcaag caattgatat acacatttcc tgaagatgct
     2821 gcgactagta ccggagctcc attctggtct gctccaaaaa gattccctcg tccactccag
     2881 tactcgtcgt ccgacccaag tctccttaac tttatcacag cgactgctat tttacgagca
     2941 gagacatttg ggatcccgat acctgagtgg actaagaacc caaaggaagc agcagaagct
     3001 gttgacagag tgatagtccc agactttgag ccaaggcaag atgcaaagat tgtgactgat
     3061 gagaaagcta ccactttaac cactgcttcg gtggacgacg ctgcagtcat cgacgatctc
     3121 attgctaaga tcgaccagtg taggcataac ttgtcgccag acttcaggat gaaacctatt
     3181 cagttcgaaa aggatgatga tacaaactat cacatggatg tgatagcagg actagccaac
     3241 atgagggcga gaaattacag catccctgaa gtggacaagc tgaaagcaaa gttcatagca
     3301 gggagaatca tcccagccat tgcgacttca acagccatgg ctactgggct ggtctgcctc
     3361 gagctgtaca aggtccttga tggaggacat aaagtggaag cttacagaaa cacatttgcc
     3421 aacctggcac ttccactatt ctccatggct gaaccacttc caccaaaggt ggtgaagcac
     3481 cgtgacatgg cttggacagt ttgggacaga tgggttctca agggaaaccc aactcttcgt
     3541 gaggtgttgc agtggctgga ggacaaaggg cttagcgcat acagcatctc ttgtggaagc
     3601 tgtctactgt tcaacagtat gttcacaagg cacaaggaga ggatggacaa gaaggttgtg
     3661 gatctcgctc gggatgttgc gaaagtcgag ttaccgcctt atcgcaacca tcttgatgta
     3721 gtggtggctt gtgaggatga agatgacaat gacgtcgata ttcctctcgt ctctatctac
     3781 ttcaggtgag cttactatta actttaagaa ggagatatac catgtaccca tacgatgttc
     3841 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggattgtat ccatatgacg
     3901 ttccagatta cgccactaga gctgcttacc catacgatgt tcctgactat gcgggctatc
     3961 cctatgacgt cccggactat gcaggattgt atccatatga cgttccagat tacgcaatgg
     4021 cttcgaaacg gatcttgaag gagctgaagg atctccagaa agaccctcca acctcctgca
     4081 gtgcaggtcc agttgctgag gacatgtttc attggcaagc tacaattatg ggtcctgcag
     4141 agagtccgta ttcaggcggt gtgtttctcg ttaccattca cttccctccg gactatccat
     4201 tcaaaccacc aaaggttgca tttaggacga aggtgtttca ccctaatatc aacagcaacg
     4261 gaagcatttg ccttgacatt ttgaaagaac aatggagccc tgccctcacc atttccaagg
     4321 tgttgctctc gatatgttcg ctgttaacag atccaaatcc agatgaccct ttggtaccag
     4381 agattgcaca catgtacaaa accgacagag ccaaatacga ggctactgca agaaactgga
     4441 ctcagaagta tgccatgggc taagctttta cttaacttta agaaggagat ataccatgtc
     4501 ccctatacta ggttattgga aaattaaggg ccttgtgcaa cccactcgac ttcttttgga
     4561 atatcttgaa gaaaaatatg aagagcattt gtatgagcgc gatgaaggtg ataaatggcg
     4621 aaacaaaaag tttgaattgg gtttggagtt tcccaatctt ccttattata ttgatggtga
     4681 tgttaaatta acacagtcta tggccatcat acgttatata gctgacaagc acaacatgtt
     4741 gggtggttgt ccaaaagagc gtgcagagat ttcaatgctt gaaggagcgg ttttggatat
     4801 tagatacggt gtttcgagaa ttgcatatag taaagacttt gaaactctca aagttgattt
     4861 tcttagcaag ctacctgaaa tgctgaaaat gttcgaagat cgtttatgtc ataaaacata
     4921 tttaaatggt gatcatgtaa cccatcctga cttcatgttg tatgacgctc ttgatgttgt
     4981 tttatacatg gacccaatgt gcctggatgc gttcccaaaa ttagtttgtt ttaaaaaacg
     5041 tattgaagct atcccacaaa ttgataagta cttgaaatcc agcaagtata tagcatggcc
     5101 tttgcagggc tggcaagcca cgtttggtgg tggcgaccat cctccaaaat cggatctggt
     5161 tccgcgtgga tcaatggcgg gtatcaatag agcgggacca tcgggcgctt attttgttgg
     5221 ccacacagac cccgagccag tatcggggca agcacacgga tccggcagcg gcgccagctc
     5281 ctcgaacagt ccgcaggttc agccgcgacc ctcgaatact cccccgtcga acgcgcccgc
     5341 accgccgcca accggacgtg agaggctttc acgatccacg gcgctgtcgc gccaaaccag
     5401 ggagtggctg gagcagggta tgcctacagc ggaggatgcc agcgtgcgtc gtaggccaca
     5461 ggtgactgcc gatgccgcaa cgccgcgtgc agaggcaaga cgcacgccgg aggcaactgc
     5521 cgatgccagc gcaccgcgta gaggggcggt tgcacacgcc aacagtatcg ttcagcaatt
     5581 ggtcagtgag ggcgctgata tttcgcatac tcgtaacatg ctccgcaatg caatgaatgg
     5641 cgacgcagtc gctttttctc gagtagaaca gaacatattt cgccagcatt tcccgaacat
     5701 gcccatgcat ggaatcagcc gagattcgga actcgctatc gagctccgtg gagcgcttcg
     5761 tcgagcggtt caccaacagg cggcgtcagc gccagtgagg tcgcccacgc caacaccggc
     5821 cagccctgcg gcatcatcat cgggcagcag tcagcgttct ttatttggac ggtttgcccg
     5881 tttgatggcg ccaaaccagg gacggtcgtc gaacactgcc gcctctcaga cgccggtcga
     5941 caggagcccg ccacgcgtca accaaagacc catacgcgtc gacagggctg cgatgcgtaa
     6001 tcgtggcaat gacgaggcgg acgccgcgct gcgggggtta gtacaacagg gggtcaattt
     6061 agagcacctg cgcacggccc ttgaaagaca tgtaatgcag cgcctcccta tccccctcga
     6121 tataggcagc gcgttgcaga atgtgggaat taacccaagt atcgacttgg gggaaagcct
     6181 tgtgcaacat cccctgctga atttgaatgt agcgttgaat cgcatgctgg ggctgcgtcc
     6241 cagcgctgaa agagcgcctc gtccagccgt ccccgtggct cccgcgaccg cctccaggcg
     6301 accggatggt acgcgtgcaa cacgattgcg ggtgatgccg gagcgggagg attacgaaaa
     6361 taatgtggcc tatggagtgc gcttgcttaa cctgaacccg ggggtggggg taaggcaggc
     6421 tgttgcggcc tttgtaaccg accgggctga gcggccagca gtggtggcta atatccgggc
     6481 agccctggac cctatcgcgt cacaattcag tcagctgcgc acaatttcga aggccgatgc
     6541 tgaatctgaa gagctgggtt ttaaggatgc ggcagatcat cacacggatg acgtgacgca
     6601 ctgtcttgcg ggcggagaat tgtcgctgag taatccggat cagcaggtga tcggtttggc
     6661 gggtaatccg acggacacgt cgcagcctta cagccaagag ggaaataagg acctggcgtt
     6721 catggatatg aaaaaacttg cccaattcct cgcaggcaag cctgagcatc cgatgaccag
     6781 agaaacgctt aacgccgaaa atatcgccaa gtatgctttt agaatagtcc cctgagcttc
     6841 agagctttta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     6901 tctgctcccg gcatccgctt acagacaagc tgtgaccggg atgagatccg gctgctaaca
     6961 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     7021 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     7081 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     7141 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     7201 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     7261 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     7321 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     7381 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     7441 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     7501 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     7561 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     7621 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     7681 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     7741 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     7801 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     7861 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     7921 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     7981 gttattcatt cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca
     8041 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     8101 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     8161 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     8221 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     8281 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     8341 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     8401 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     8461 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     8521 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     8581 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     8641 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     8701 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     8761 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     8821 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     8881 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     8941 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     9001 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     9061 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     9121 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     9181 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     9241 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     9301 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     9361 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     9421 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     9481 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     9541 ccggcatccg cttacagaca agctgtgacc gactccggga gctgcatgtg tcagaggttt
     9601 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     9661 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     9721 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     9781 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     9841 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     9901 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     9961 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
    10021 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
    10081 acacggaaac ccaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
    10141 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
    10201 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
    10261 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
    10321 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
    10381 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
    10441 tgcatgataa agaacacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
    10501 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
    10561 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
    10621 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
    10681 cgccagggtg gtttttcttt tcaccagtga cacgggcaac agctgattgc ccttcaccgc
    10741 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
    10801 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcctcggtat cgtcgtatcc
    10861 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
    10921 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
    10981 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
    11041 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
    11101 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
    11161 cacgcccagt cgcgtaccgt cctcatggga gaaaataata ctgttgatgg gtgtctggtc
    11221 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
    11281 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
    11341 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
    11401 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
    11461 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
    11521 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
    11581 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
    11641 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
    11701 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
    11761 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
    11821 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
    11881 ccacggggcc tgccacc
//
