LOCUS       sequence-205463-       11519 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11519)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 11519)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-153
FEATURES             Location/Qualifiers
     source          1..11519
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             3..20
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3574..3591
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3861..4514
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             4521..4538
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(6359..6377)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6373..6420
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6457..6912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6544..6563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6754..6775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7004..7819)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7004..7819)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7727..7746
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7811..7830
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7941..8529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8430..8449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8683..8700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8715..8854
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8780..8799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8940..8962
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8956..9147)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(9956..11038)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     11000..11019
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(11039..11116)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     11322..11341
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     11425..11444
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11425..11443
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    11444..11468
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gccaccacca ccaccaccac agcggaatgc agatctttgt taagactctc accggaaaga
       61 caatcaccct agaggtggaa agctccgaca ccatcgacaa cgttaaggcc aagatccagg
      121 ataaggaggg cattcctccg gatcagcaga ggcttatttt cgccggcaag cagctagagg
      181 atggccgtac gttggctgat tacaatatcc agaaggaatc caccctccac ttggtcctca
      241 ggctccgtgg tggttaagct tgcaattaac tttaagaagg agatatacaa tgcttcacaa
      301 gcgagctagt gaagctaacg ataagaacga taataccatc atcggttccg atttagcatc
      361 atctaagaaa cgccgtatcg atttcactga atcttcgtct gataagtcat cttcgatttt
      421 agcttctggt agtagcaggg gttttcacgg cgatagcgtc gttcagcaaa tcgacatggc
      481 ttttggtaat tcgaaccgtc aggagattga tgaagatctg cacagtcgcc agctcgccgt
      541 ctatggtcgt gagactatga ggcgtctctt tgcttccaat gttctcatct cggggatgca
      601 cggtcttggt gccgagattg ccaagaatct tatacttgct ggtgtgaagt ctgtgacctt
      661 gcatgatgaa agagttgtag agctatggga cttatcaagc aactttgttt tctcggagga
      721 tgatgttggc aagaatagag cagatgcttc tgttcagaag ttacaggatc ttaacaatgc
      781 tgtggttgtc tctagcttga ccaaaagctt aaacaaagag gatctttctg gcttccaggt
      841 tgttgtattc tctgacataa gcatggaaag agcaatcgaa tttgatgact attgtcacag
      901 ccatcagcct cctatcgctt ttgtcaaggc tgatgtcagg ggtcttttcg gctctgtctt
      961 ttgtgatttt gggcctgaat ttgcggttct tgatgttgat ggagaggaac cccatacagg
     1021 catcattgcg tctatctcta atgagaacca ggcatttatt tcctgtgtcg acgatgagag
     1081 gcttgagttt gaagatggtg acctagttgt tttctcagaa gttgagggta tgacagaatt
     1141 gaatgatggg aaaccaagga agataaaaag tacacggcca tattcattca cccttgatga
     1201 ggacactact aactatggaa catatgtgaa gggtggtatt gtcactcaag tgaaacagcc
     1261 aaagttactg aatttcaagc cattgaggga agcgcttaag gatccagggg attttctatt
     1321 tagtgatttc tctaagtttg atcggcctcc actccttcac ttagcattcc aggcacttga
     1381 tcattttaaa gctgaagctg gcagatttcc tgttgctggc tcagaagagg acgctcagaa
     1441 gcttatatcg attgccacag ccatcaatac cgggcagggt gatttaaagg tggagaatgt
     1501 tgatcagaag cttctaagac acttttcctt tggagccaag gctgtcctga atcctatggc
     1561 tgcaatgttt ggcggtattg ttggacagga ggttgttaaa gcttgctctg gaaaatttca
     1621 tcctctcttc cagtttttct actttgattc agtggagtca ctcccctctg aacctgtgga
     1681 ctcgagtgac tttgcaccaa gaaacagccg gtatgatgcc caaatatctg tatttggggc
     1741 caagttccag aagaaacttg aagatgctaa agttttcaca gtagggtctg gtgctcttgg
     1801 ctgtgagttc ttgaaaaatt tggctctgat gggggtttct tgtggaagcc aagggaagct
     1861 gacagtgaca gatgatgata tcattgagaa gagtaacctc agtcgtcaat ttctgttccg
     1921 tgactggaac attggacagg ctaaatccac agttgctgct tcggctgctg ctgttataaa
     1981 tcctcgcttc aacattgagg ccctgcaaaa ccgtgttggc gcggagactg agaatgtatt
     2041 tgacgatgcc ttctgggaga acttaactgt tgtcgtcaat gcactggata atgttaatgc
     2101 gaggctctat gttgattcga ggtgcttgta tttccagaag cctctccttg agtctggaac
     2161 tcttggtaca aagtgcaaca cacagtcggt tatcccacat ctaactgaaa attacggtgc
     2221 ctcaagggac ccaccagaga aacaggcccc catgtgtaca gtgcattcct tcccgcataa
     2281 cattgatcac tgtttaactt gggctcgctc tgagtttgag ggtctgctgg agaaaactcc
     2341 cgctgaagtg aatgcatatc tttctagccc agttgagtac actaactcaa tgatgagtgc
     2401 tggtgatgct caggcgaggg acacattgga gaggattgtt gagtgccttg aaaaggagaa
     2461 gtgtgaaacc ttccaagact gcttaacctg ggcacgactg aggtttgagg attactttgt
     2521 gaaccgtgtc aagcaattga tatacacatt tcctgaagat gctgcgacta gtaccggagc
     2581 tccattctgg tctgctccaa aaagattccc tcgtccactc cagtactcgt cgtccgaccc
     2641 aagtctcctt aactttatca cagcgactgc tattttacga gcagagacat ttgggatccc
     2701 gatacctgag tggactaaga acccaaagga agcagcagaa gctgttgaca gagtgatagt
     2761 cccagacttt gagccaaggc aagatgcaaa gattgtgact gatgagaaag ctaccacttt
     2821 aaccactgct tcggtggacg acgctgcagt catcgacgat ctcattgcta agatcgacca
     2881 gtgtaggcat aacttgtcgc cagacttcag gatgaaacct attcagttcg aaaaggatga
     2941 tgatacaaac tatcacatgg atgtgatagc aggactagcc aacatgaggg cgagaaatta
     3001 cagcatccct gaagtggaca agctgaaagc aaagttcata gcagggagaa tcatcccagc
     3061 cattgcgact tcaacagcca tggctactgg gctggtctgc ctcgagctgt acaaggtcct
     3121 tgatggagga cataaagtgg aagcttacag aaacacattt gccaacctgg cacttccact
     3181 attctccatg gctgaaccac ttccaccaaa ggtggtgaag caccgtgaca tggcttggac
     3241 agtttgggac agatgggttc tcaagggaaa cccaactctt cgtgaggtgt tgcagtggct
     3301 ggaggacaaa gggcttagcg catacagcat ctcttgtgga agctgtctac tgttcaacag
     3361 tatgttcaca aggcacaagg agaggatgga caagaaggtt gtggatctcg ctcgggatgt
     3421 tgcgaaagtc gagttaccgc cttatcgcaa ccatcttgat gtagtggtgg cttgtgagga
     3481 tgaagatgac aatgacgtcg atattcctct cgtctctatc tacttcaggt gagcttacta
     3541 ttaactttaa gaaggagata taccatgggc agccaccacc accaccacca cagcggaatg
     3601 cagatctttg ttaagactct caccggaaag acaatcaccc tagaggtgga aagctccgac
     3661 accatcgaca acgttaaggc caagatccag gataaggagg gcattcctcc ggatcagcag
     3721 aggcttattt tcgccggcaa gcagctagag gatggccgta cgttggctga ttacaatatc
     3781 cagaaggaat ccaccctcca cttggtcctc aggctccgtg gtggttaagc ttttacttaa
     3841 ctttaagaag gagatatacc atgtccccta tactaggtta ttggaaaatt aagggccttg
     3901 tgcaacccac tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg
     3961 agcgcgatga aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca
     4021 atcttcctta ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt
     4081 atatagctga caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa
     4141 tgcttgaagg agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag
     4201 actttgaaac tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg
     4261 aagatcgttt atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca
     4321 tgttgtatga cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc
     4381 caaaattagt ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga
     4441 aatccagcaa gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggcg
     4501 accatcctcc aaaatcggat ctggttccgc gtggatcaat ggcgggtatc aatagagcgg
     4561 gaccatcggg cgcttatttt gttggccaca cagaccccga gccagtatcg gggcaagcac
     4621 acggatccgg cagcggcgcc agctcctcga acagtccgca ggttcagccg cgaccctcga
     4681 atactccccc gtcgaacgcg cccgcaccgc cgccaaccgg acgtgagagg ctttcacgat
     4741 ccacggcgct gtcgcgccaa accagggagt ggctggagca gggtatgcct acagcggagg
     4801 atgccagcgt gcgtcgtagg ccacaggtga ctgccgatgc cgcaacgccg cgtgcagagg
     4861 caagacgcac gccggaggca actgccgatg ccagcgcacc gcgtagaggg gcggttgcac
     4921 acgccaacag tatcgttcag caattggtca gtgagggcgc tgatatttcg catactcgta
     4981 acatgctccg caatgcaatg aatggcgacg cagtcgcttt ttctcgagta gaacagaaca
     5041 tatttcgcca gcatttcccg aacatgccca tgcatggaat cagccgagat tcggaactcg
     5101 ctatcgagct ccgtggagcg cttcgtcgag cggttcacca acaggcggcg tcagcgccag
     5161 tgaggtcgcc cacgccaaca ccggccagcc ctgcggcatc atcatcgggc agcagtcagc
     5221 gttctttatt tggacggttt gcccgtttga tggcgccaaa ccagggacgg tcgtcgaaca
     5281 ctgccgcctc tcagacgccg gtcgacagga gcccgccacg cgtcaaccaa agacccatac
     5341 gcgtcgacag ggctgcgatg cgtaatcgtg gcaatgacga ggcggacgcc gcgctgcggg
     5401 ggttagtaca acagggggtc aatttagagc acctgcgcac ggcccttgaa agacatgtaa
     5461 tgcagcgcct ccctatcccc ctcgatatag gcagcgcgtt gcagaatgtg ggaattaacc
     5521 caagtatcga cttgggggaa agccttgtgc aacatcccct gctgaatttg aatgtagcgt
     5581 tgaatcgcat gctggggctg cgtcccagcg ctgaaagagc gcctcgtcca gccgtccccg
     5641 tggctcccgc gaccgcctcc aggcgaccgg atggtacgcg tgcaacacga ttgcgggtga
     5701 tgccggagcg ggaggattac gaaaataatg tggcctatgg agtgcgcttg cttaacctga
     5761 acccgggggt gggggtaagg caggctgttg cggcctttgt aaccgaccgg gctgagcggc
     5821 cagcagtggt ggctaatatc cgggcagccc tggaccctat cgcgtcacaa ttcagtcagc
     5881 tgcgcacaat ttcgaaggcc gatgctgaat ctgaagagct gggttttaag gatgcggcag
     5941 atcatcacac ggatgacgtg acgcactgtc tttttggcgg agaattgtcg ctgagtaatc
     6001 cggatcagca ggtgatcggt ttggcgggta atccgacgga cacgtcgcag ccttacagcc
     6061 aagagggaaa taaggacctg gcgttcatgg atatgaaaaa acttgcccaa ttcctcgcag
     6121 gcaagcctga gcatccgatg accagagaaa cgcttaacgc cgaaaatatc gccaagtatg
     6181 cttttagaat agtcccctga gcttcagagc ttttaagcca gccccgacac ccgccaacac
     6241 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6301 ccgggatgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     6361 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     6421 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
     6481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6901 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6961 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
     7021 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     7081 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     7141 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     7201 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     7261 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     7321 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa
     7381 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     7441 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     7501 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     7561 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     7621 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     7681 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     7741 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     7801 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     7861 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8701 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     8761 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     8821 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     8881 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgactc
     8941 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     9001 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     9061 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     9121 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     9181 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     9241 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     9301 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     9361 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     9421 cttccgcgtt tccagacttt acgaaacacg gaaacccaag accattcatg ttgttgctca
     9481 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     9541 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     9601 catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     9661 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     9721 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     9781 cgctgccggc acctgtccta cgagttgcat gataaagaac acagtcataa gtgcggcgac
     9841 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     9901 cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact
     9961 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10021 ggggagaggc ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgacacgg
    10081 gcaacagctg attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc
    10141 tggtttgccc cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg
    10201 agctgtcctc ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg
    10261 actcggtaat ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag
    10321 tgggaacgat gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc
    10381 agtcgccttc ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc
    10441 cagccagacg cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct
    10501 ggtgacccaa tgcgaccaga tgctccacgc ccagtcgcgt accgtcctca tgggagaaaa
    10561 taatactgtt gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc
    10621 aggcagcttc cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac
    10681 tgacgcgttg cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt
    10741 ctaccatcga caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga
    10801 caatttgcga cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact
    10861 gtttgcccgc cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg
    10921 cttccacttt ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa
    10981 cggtctgata agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat
    11041 tcaccaccct gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc
    11101 gccattcgat ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag
    11161 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag
    11221 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa
    11281 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag
    11341 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg
    11401 atcgagatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
    11461 acaattcccc tctagaaata attttgcctt aactttaaga aggagatata ccatgggca
//
