LOCUS       sequence-205465-       13009 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 13009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-413
FEATURES             Location/Qualifiers
     source          1..13009
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     8..27
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     111..130
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        111..129
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    130..154
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             208..225
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3773..3862
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3875..3964
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4444..5097
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             5104..5121
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             6819..6908
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             6849..6938
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(8054..8072)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8068..8115
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8152..8607
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8239..8258)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8449..8470
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8699..9514)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8699..9514)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9422..9441
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9506..9525
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9636..10224
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10125..10144
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10378..10395
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10410..10549
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10475..10494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10635..10657
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10651..10842)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(11651..12733)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12695..12714
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(12734..12811)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
       61 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
      121 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgccttaact
      181 ttaagaagga gatataccat gggcagccac caccaccacc accacagcgg aatgcagatc
      241 tttgttaaga ctctcaccgg aaagacaatc accctagagg tggaaagctc cgacaccatc
      301 gacaacgtta aggccaagat ccaggataag gagggcattc ctccggatca gcagaggctt
      361 attttcgccg gcaagcagct agaggatggc cgtacgttgg ctgattacaa tatccagaag
      421 gaatccaccc tccacttggt cctcaggctc cgtggtggtt aagcttgcaa ttaactttaa
      481 gaaggagata tacaatgctt cacaagcgag ctagtgaagc taacgataag aacgataata
      541 ccatcatcgg ttccgattta gcatcatcta agaaacgccg tatcgatttc actgaatctt
      601 cgtctgataa gtcatcttcg attttagctt ctggtagtag caggggtttt cacggcgata
      661 gcgtcgttca gcaaatcgac atggcttttg gtaattcgaa ccgtcaggag attgatgaag
      721 atctgcacag tcgccagctc gccgtctatg gtcgtgagac tatgaggcgt ctctttgctt
      781 ccaatgttct catctcgggg atgcacggtc ttggtgccga gattgccaag aatcttatac
      841 ttgctggtgt gaagtctgtg accttgcatg atgaaagagt tgtagagcta tgggacttat
      901 caagcaactt tgttttctcg gaggatgatg ttggcaagaa tagagcagat gcttctgttc
      961 agaagttaca ggatcttaac aatgctgtgg ttgtctctag cttgaccaaa agcttaaaca
     1021 aagaggatct ttctggcttc caggttgttg tattctctga cataagcatg gaaagagcaa
     1081 tcgaatttga tgactattgt cacagccatc agcctcctat cgcttttgtc aaggctgatg
     1141 tcaggggtct tttcggctct gtcttttgtg attttgggcc tgaatttgcg gttcttgatg
     1201 ttgatggaga ggaaccccat acaggcatca ttgcgtctat ctctaatgag aaccaggcat
     1261 ttatttcctg tgtcgacgat gagaggcttg agtttgaaga tggtgaccta gttgttttct
     1321 cagaagttga gggtatgaca gaattgaatg atgggaaacc aaggaagata aaaagtacac
     1381 ggccatattc attcaccctt gatgaggaca ctactaacta tggaacatat gtgaagggtg
     1441 gtattgtcac tcaagtgaaa cagccaaagt tactgaattt caagccattg agggaagcgc
     1501 ttaaggatcc aggggatttt ctatttagtg atttctctaa gtttgatcgg cctccactcc
     1561 ttcacttagc attccaggca cttgatcatt ttaaagctga agctggcaga tttcctgttg
     1621 ctggctcaga agaggacgct cagaagctta tatcgattgc cacagccatc aataccgggc
     1681 agggtgattt aaaggtggag aatgttgatc agaagcttct aagacacttt tcctttggag
     1741 ccaaggctgt cctgaatcct atggctgcaa tgtttggcgg tattgttgga caggaggttg
     1801 ttaaagcttg ctctggaaaa tttcatcctc tcttccagtt tttctacttt gattcagtgg
     1861 agtcactccc ctctgaacct gtggactcga gtgactttgc accaagaaac agccggtatg
     1921 atgcccaaat atctgtattt ggggccaagt tccagaagaa acttgaagat gctaaagttt
     1981 tcacagtagg gtctggtgct cttggctgtg agttcttgaa aaatttggct ctgatggggg
     2041 tttcttgtgg aagccaaggg aagctgacag tgacagatga tgatatcatt gagaagagta
     2101 acctcagtcg tcaatttctg ttccgtgact ggaacattgg acaggctaaa tccacagttg
     2161 ctgcttcggc tgctgctgtt ataaatcctc gcttcaacat tgaggccctg caaaaccgtg
     2221 ttggcgcgga gactgagaat gtatttgacg atgccttctg ggagaactta actgttgtcg
     2281 tcaatgcact ggataatgtt aatgcgaggc tctatgttga ttcgaggtgc ttgtatttcc
     2341 agaagcctct ccttgagtct ggaactcttg gtacaaagtg caacacacag tcggttatcc
     2401 cacatctaac tgaaaattac ggtgcctcaa gggacccacc agagaaacag gcccccatgt
     2461 gtacagtgca ttccttcccg cataacattg atcactgttt aacttgggct cgctctgagt
     2521 ttgagggtct gctggagaaa actcccgctg aagtgaatgc atatctttct agcccagttg
     2581 agtacactaa ctcaatgatg agtgctggtg atgctcaggc gagggacaca ttggagagga
     2641 ttgttgagtg ccttgaaaag gagaagtgtg aaaccttcca agactgctta acctgggcac
     2701 gactgaggtt tgaggattac tttgtgaacc gtgtcaagca attgatatac acatttcctg
     2761 aagatgctgc gactagtacc ggagctccat tctggtctgc tccaaaaaga ttccctcgtc
     2821 cactccagta ctcgtcgtcc gacccaagtc tccttaactt tatcacagcg actgctattt
     2881 tacgagcaga gacatttggg atcccgatac ctgagtggac taagaaccca aaggaagcag
     2941 cagaagctgt tgacagagtg atagtcccag actttgagcc aaggcaagat gcaaagattg
     3001 tgactgatga gaaagctacc actttaacca ctgcttcggt ggacgacgct gcagtcatcg
     3061 acgatctcat tgctaagatc gaccagtgta ggcataactt gtcgccagac ttcaggatga
     3121 aacctattca gttcgaaaag gatgatgata caaactatca catggatgtg atagcaggac
     3181 tagccaacat gagggcgaga aattacagca tccctgaagt ggacaagctg aaagcaaagt
     3241 tcatagcagg gagaatcatc ccagccattg cgacttcaac agccatggct actgggctgg
     3301 tctgcctcga gctgtacaag gtccttgatg gaggacataa agtggaagct tacagaaaca
     3361 catttgccaa cctggcactt ccactattct ccatggctga accacttcca ccaaaggtgg
     3421 tgaagcaccg tgacatggct tggacagttt gggacagatg ggttctcaag ggaaacccaa
     3481 ctcttcgtga ggtgttgcag tggctggagg acaaagggct tagcgcatac agcatctctt
     3541 gtggaagctg tctactgttc aacagtatgt tcacaaggca caaggagagg atggacaaga
     3601 aggttgtgga tctcgctcgg gatgttgcga aagtcgagtt accgccttat cgcaaccatc
     3661 ttgatgtagt ggtggcttgt gaggatgaag atgacaatga cgtcgatatt cctctcgtct
     3721 ctatctactt caggtgagct tactattaac tttaagaagg agatatacca tgtacccata
     3781 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gattgtatcc
     3841 atatgacgtt ccagattacg ccactagagc tgcttaccca tacgatgttc ctgactatgc
     3901 gggctatccc tatgacgtcc cggactatgc aggattgtat ccatatgacg ttccagatta
     3961 cgcaatggct tcgaaacgga tcttgaagga gctgaaggat ctccagaaag accctccaac
     4021 ctcctgcagt gcaggtccag ttgctgagga catgtttcat tggcaagcta caattatggg
     4081 tcctgcagag agtccgtatt caggcggtgt gtttctcgtt accattcact tccctccgga
     4141 ctatccattc aaaccaccaa aggttgcatt taggacgaag gtgtttcacc ctaatatcaa
     4201 cagcaacgga agcatttgcc ttgacatttt gaaagaacaa tggagccctg ccctcaccat
     4261 ttccaaggtg ttgctctcga tatgttcgct gttaacagat ccaaatccag atgacccttt
     4321 ggtaccagag attgcacaca tgtacaaaac cgacagagcc aaatacgagg ctactgcaag
     4381 aaactggact cagaagtatg ccatgggcta agcttttact taactttaag aaggagatat
     4441 accatgtccc ctatactagg ttattggaaa attaagggcc ttgtgcaacc cactcgactt
     4501 cttttggaat atcttgaaga aaaatatgaa gagcatttgt atgagcgcga tgaaggtgat
     4561 aaatggcgaa acaaaaagtt tgaattgggt ttggagtttc ccaatcttcc ttattatatt
     4621 gatggtgatg ttaaattaac acagtctatg gccatcatac gttatatagc tgacaagcac
     4681 aacatgttgg gtggttgtcc aaaagagcgt gcagagattt caatgcttga aggagcggtt
     4741 ttggatatta gatacggtgt ttcgagaatt gcatatagta aagactttga aactctcaaa
     4801 gttgattttc ttagcaagct acctgaaatg ctgaaaatgt tcgaagatcg tttatgtcat
     4861 aaaacatatt taaatggtga tcatgtaacc catcctgact tcatgttgta tgacgctctt
     4921 gatgttgttt tatacatgga cccaatgtgc ctggatgcgt tcccaaaatt agtttgtttt
     4981 aaaaaacgta ttgaagctat cccacaaatt gataagtact tgaaatccag caagtatata
     5041 gcatggcctt tgcagggctg gcaagccacg tttggtggtg gcgaccatcc tccaaaatcg
     5101 gatctggttc cgcgtggatc aatggcgggt atcaatagag cgggaccatc gggcgcttat
     5161 tttgttggcc acacagaccc cgagccagta tcggggcaag cacacggatc cggcagcggc
     5221 gccagctcct cgaacagtcc gcaggttcag ccgcgaccct cgaatactcc cccgtcgaac
     5281 gcgcccgcac cgccgccaac cggacgtgag aggctttcac gatccacggc gctgtcgcgc
     5341 caaaccaggg agtggctgga gcagggtatg cctacagcgg aggatgccag cgtgcgtcgt
     5401 aggccacagg tgactgccga tgccgcaacg ccgcgtgcag aggcaagacg cacgccggag
     5461 gcaactgccg atgccagcgc accgcgtaga ggggcggttg cacacgccaa cagtatcgtt
     5521 cagcaattgg tcagtgaggg cgctgatatt tcgcatactc gtaacatgct ccgcaatgca
     5581 atgaatggcg acgcagtcgc tttttctcga gtagaacaga acatatttcg ccagcatttc
     5641 ccgaacatgc ccatgcatgg aatcagccga gattcggaac tcgctatcga gctccgtgga
     5701 gcgcttcgtc gagcggttca ccaacaggcg gcgtcagcgc cagtgaggtc gcccacgcca
     5761 acaccggcca gccctgcggc atcatcatcg ggcagcagtc agcgttcttt atttggacgg
     5821 tttgcccgtt tgatggcgcc aaaccaggga cggtcgtcga acactgccgc ctctcagacg
     5881 ccggtcgaca ggagcccgcc acgcgtcaac caaagaccca tacgcgtcga cagggctgcg
     5941 atgcgtaatc gtggcaatga cgaggcggac gccgcgctgc gggggttagt acaacagggg
     6001 gtcaatttag agcacctgcg cacggccctt gaaagacatg taatgcagcg cctccctatc
     6061 cccctcgata taggcagcgc gttgcagaat gtgggaatta acccaagtat cgacttgggg
     6121 gaaagccttg tgcaacatcc cctgctgaat ttgaatgtag cgttgaatcg catgctgggg
     6181 ctgcgtccca gcgctgaaag agcgcctcgt ccagccgtcc ccgtggctcc cgcgaccgcc
     6241 tccaggcgac cggatggtac gcgtgcaaca cgattgcggg tgatgccgga gcgggaggat
     6301 tacgaaaata atgtggccta tggagtgcgc ttgcttaacc tgaacccggg ggtgggggta
     6361 aggcaggctg ttgcggcctt tgtaaccgac cgggctgagc ggccagcagt ggtggctaat
     6421 atccgggcag ccctggaccc tatcgcgtca caattcagtc agctgcgcac aatttcgaag
     6481 gccgatgctg aatctgaaga gctgggtttt aaggatgcgg cagatcatca cacggatgac
     6541 gtgacgcact gtcttgcggg cggagaattg tcgctgagta atccggatca gcaggtgatc
     6601 ggtttggcgg gtaatccgac ggacacgtcg cagccttaca gccaagaggg aaataaggac
     6661 ctggcgttca tggatatgaa aaaacttgcc caattcctcg caggcaagcc tgagcatccg
     6721 atgaccagag aaacgcttaa cgccgaaaat atcgccaagt atgcttttag aatagtcccc
     6781 tgagcttcag attaacttta agaaggagat ataccatgga acaaaagttg atctctgaag
     6841 aggaccttga gcagaaattg atctctgagg aagatcttga gcagaagctt atctcagagg
     6901 aggatttgga gcaaaaactt atttctgaag aggacctaat gggaagcaag tattccaagg
     6961 caacaaattc gataaatgat gcttctaact tgagttatgg cgttcctttt gaaaattatc
     7021 gagttccttt tgtagatttg gaggaagcaa ctaacaactt tgatgacaat tttttcattg
     7081 gagagggtgg atttgggaag gtttacaggg gtgttttgcg tgatggaaca aaggtcgccc
     7141 tgaaaaagca taaacctgag tcctcacaag gtattgaaga gttcgaaaca gaaattgaga
     7201 ttctctcatt ttgcagccat ccgcatctgg tttcattgat aggattctgt gatgaaagaa
     7261 atgagatgat tctaatttat gactacatgg agaatgggaa cctcaagagc catttgtatg
     7321 gatcagatct accctccatg agctgggagc agaggctgga gatatgtatc ggggcagcca
     7381 gaggccttca ctaccttcat aagaatgcag ttatacatcg tgatgtcaag tgtacaaaca
     7441 tattgcttga tgagaatttt gtgccaaaaa ttactgattt tggaatatct aagacaatgc
     7501 ccgagcttga ccaaacccat cttagcacag tagtgagagg aaatataggc tacattgccc
     7561 ctgaatatgc tttgtgggga cagctgacag aaaaatctga tgtttattct ttcggtgttg
     7621 ttttattcga agttctttgt gctaggcctg ccttagatcg ttcagagata atgagtttag
     7681 atgatgagac gcagaagatg ggacagttgg aacaaattgt agatcccact attgcggcca
     7741 aaataagacc agagtccctc aggatgtttg gagaaacagc gatcaaatgc ttagctccgt
     7801 ctagtaaaaa taggccatca atgggtgatg tgctgtggaa actggagtat gcactttgtc
     7861 tccaagagcc taccattcaa gatgatcctg aatgagcttt gtggctttta agccagcccc
     7921 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     7981 acagacaagc tgtgaccggg atgagatccg gctgctaaca aagcccgaaa ggaagctgag
     8041 ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc
     8101 ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg gacgcgccct
     8161 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     8221 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     8281 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     8341 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     8401 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     8461 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     8521 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     8581 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     8641 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga attaattctt
     8701 agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga ttatcaatac
     8761 catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg cagttccata
     8821 ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca atacaaccta
     8881 ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga gtgacgactg
     8941 aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca acaggccagc
     9001 cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt cgtgattgcg
     9061 cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca attacaaaca ggaatcgaat
     9121 gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa tcaggatatt
     9181 cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac catgcatcat
     9241 caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc agccagttta
     9301 gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt ttcagaaaca
     9361 actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat tgcccgacat
     9421 tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt aatcgcggcc
     9481 tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta ctgtttatgt
     9541 aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc gttccactga
     9601 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     9661 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     9721 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     9781 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     9841 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     9901 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     9961 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
    10021 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
    10081 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
    10141 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
    10201 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
    10261 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
    10321 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
    10381 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg
    10441 tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    10501 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
    10561 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
    10621 agctgtgacc gactccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
    10681 cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc
    10741 ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat
    10801 aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg
    10861 gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg
    10921 ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt
    10981 atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac
    11041 agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat
    11101 ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac ccaagaccat
    11161 tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg
    11221 tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa
    11281 cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa tggcctgctt
    11341 ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat
    11401 tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc
    11461 gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaacacagt
    11521 cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa
    11581 ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac ttacattaat
    11641 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg
    11701 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg gtttttcttt
    11761 tcaccagtga cacgggcaac agctgattgc ccttcaccgc ctggccctga gagagttgca
    11821 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
    11881 gcgggatata acatgagctg tcctcggtat cgtcgtatcc cactaccgag atatccgcac
    11941 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
    12001 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
    12061 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
    12121 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
    12181 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
    12241 cctcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
    12301 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
    12361 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
    12421 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
    12481 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
    12541 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
    12601 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
    12661 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
    12721 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
    12781 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
    12841 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
    12901 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
    12961 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatctt
//
