LOCUS       sequence-205466-       12631 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 12631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-417
FEATURES             Location/Qualifiers
     source          1..12631
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     875..894
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(914..991)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1197..1216
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1300..1319
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1300..1318
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1319..1343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1397..1414
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4968..4985
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5255..5908
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     CDS             5915..5932
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             7630..7719
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     CDS             7660..7749
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     primer_bind     complement(8865..8883)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8879..8926
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8963..9418
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9050..9069)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9260..9281
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(9510..10325)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9510..10325)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10233..10252
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10317..10336
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10447..11035
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10936..10955
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11189..11206
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    11221..11360
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(11286..11305)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11446..11468
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(11462..11653)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(12462..12631,1..913))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg
       61 gcgggatata acatgagctg tcctcggtat cgtcgtatcc cactaccgag atatccgcac
      121 caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg
      181 caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt tgttgaaaac
      241 cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga
      301 gatatttatg ccagccagcc agacgcagac gcgccgagac agaacttaat gggcccgcta
      361 acagcgcgat ttgctggtga cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt
      421 cctcatggga gaaaataata ctgttgatgg gtgtctggtc agagacatca agaaataacg
      481 ccggaacatt agtgcaggca gcttccacag caatggcatc ctggtcatcc agcggatagt
      541 taatgatcag cccactgacg cgttgcgcga gaagattgtg caccgccgct ttacaggctt
      601 cgacgccgct tcgttctacc atcgacacca ccacgctggc acccagttga tcggcgcgag
      661 atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc cagactggag gtggcaacgc
      721 caatcagcaa cgactgtttg cccgccagtt gttgtgccac gcggttggga atgtaattca
      781 gctccgccat cgccgcttcc actttttccc gcgttttcgc agaaacgtgg ctggcctggt
      841 tcaccacgcg ggaaacggtc tgataagaga caccggcata ctctgcgaca tcgtataacg
      901 ttactggttt cacattcacc accctgaatt gactctcttc cgggcgctat catgccatac
      961 cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc gacgctctcc cttatgcgac
     1021 tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg
     1081 aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata
     1141 cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg
     1201 atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg
     1261 cgtccggcgt agaggatcga gatctcgatc ccgcgaaatt aatacgactc actatagggg
     1321 aattgtgagc ggataacaat tcccctctag aaataatttt gccttaactt taagaaggag
     1381 atataccatg ggcagccacc accaccacca ccacagcgga atgcagatct ttgttaagac
     1441 tctcaccgga aagacaatca ccctagaggt ggaaagctcc gacaccatcg acaacgttaa
     1501 ggccaagatc caggataagg agggcattcc tccggatcag cagaggctta ttttcgccgg
     1561 caagcagcta gaggatggcc gtacgttggc tgattacaat atccagaagg aatccaccct
     1621 ccacttggtc ctcaggctcc gtggtggtta agcttgcaat taactttaag aaggagatat
     1681 acaatgcttc acaagcgagc tagtgaagct aacgataaga acgataatac catcatcggt
     1741 tccgatttag catcatctaa gaaacgccgt atcgatttca ctgaatcttc gtctgataag
     1801 tcatcttcga ttttagcttc tggtagtagc aggggttttc acggcgatag cgtcgttcag
     1861 caaatcgaca tggcttttgg taattcgaac cgtcaggaga ttgatgaaga tctgcacagt
     1921 cgccagctcg ccgtctatgg tcgtgagact atgaggcgtc tctttgcttc caatgttctc
     1981 atctcgggga tgcacggtct tggtgccgag attgccaaga atcttatact tgctggtgtg
     2041 aagtctgtga ccttgcatga tgaaagagtt gtagagctat gggacttatc aagcaacttt
     2101 gttttctcgg aggatgatgt tggcaagaat agagcagatg cttctgttca gaagttacag
     2161 gatcttaaca atgctgtggt tgtctctagc ttgaccaaaa gcttaaacaa agaggatctt
     2221 tctggcttcc aggttgttgt attctctgac ataagcatgg aaagagcaat cgaatttgat
     2281 gactattgtc acagccatca gcctcctatc gcttttgtca aggctgatgt caggggtctt
     2341 ttcggctctg tcttttgtga ttttgggcct gaatttgcgg ttcttgatgt tgatggagag
     2401 gaaccccata caggcatcat tgcgtctatc tctaatgaga accaggcatt tatttcctgt
     2461 gtcgacgatg agaggcttga gtttgaagat ggtgacctag ttgttttctc agaagttgag
     2521 ggtatgacag aattgaatga tgggaaacca aggaagataa aaagtacacg gccatattca
     2581 ttcacccttg atgaggacac tactaactat ggaacatatg tgaagggtgg tattgtcact
     2641 caagtgaaac agccaaagtt actgaatttc aagccattga gggaagcgct taaggatcca
     2701 ggggattttc tatttagtga tttctctaag tttgatcggc ctccactcct tcacttagca
     2761 ttccaggcac ttgatcattt taaagctgaa gctggcagat ttcctgttgc tggctcagaa
     2821 gaggacgctc agaagcttat atcgattgcc acagccatca ataccgggca gggtgattta
     2881 aaggtggaga atgttgatca gaagcttcta agacactttt cctttggagc caaggctgtc
     2941 ctgaatccta tggctgcaat gtttggcggt attgttggac aggaggttgt taaagcttgc
     3001 tctggaaaat ttcatcctct cttccagttt ttctactttg attcagtgga gtcactcccc
     3061 tctgaacctg tggactcgag tgactttgca ccaagaaaca gccggtatga tgcccaaata
     3121 tctgtatttg gggccaagtt ccagaagaaa cttgaagatg ctaaagtttt cacagtaggg
     3181 tctggtgctc ttggctgtga gttcttgaaa aatttggctc tgatgggggt ttcttgtgga
     3241 agccaaggga agctgacagt gacagatgat gatatcattg agaagagtaa cctcagtcgt
     3301 caatttctgt tccgtgactg gaacattgga caggctaaat ccacagttgc tgcttcggct
     3361 gctgctgtta taaatcctcg cttcaacatt gaggccctgc aaaaccgtgt tggcgcggag
     3421 actgagaatg tatttgacga tgccttctgg gagaacttaa ctgttgtcgt caatgcactg
     3481 gataatgtta atgcgaggct ctatgttgat tcgaggtgct tgtatttcca gaagcctctc
     3541 cttgagtctg gaactcttgg tacaaagtgc aacacacagt cggttatccc acatctaact
     3601 gaaaattacg gtgcctcaag ggacccacca gagaaacagg cccccatgtg tacagtgcat
     3661 tccttcccgc ataacattga tcactgttta acttgggctc gctctgagtt tgagggtctg
     3721 ctggagaaaa ctcccgctga agtgaatgca tatctttcta gcccagttga gtacactaac
     3781 tcaatgatga gtgctggtga tgctcaggcg agggacacat tggagaggat tgttgagtgc
     3841 cttgaaaagg agaagtgtga aaccttccaa gactgcttaa cctgggcacg actgaggttt
     3901 gaggattact ttgtgaaccg tgtcaagcaa ttgatataca catttcctga agatgctgcg
     3961 actagtaccg gagctccatt ctggtctgct ccaaaaagat tccctcgtcc actccagtac
     4021 tcgtcgtccg acccaagtct ccttaacttt atcacagcga ctgctatttt acgagcagag
     4081 acatttggga tcccgatacc tgagtggact aagaacccaa aggaagcagc agaagctgtt
     4141 gacagagtga tagtcccaga ctttgagcca aggcaagatg caaagattgt gactgatgag
     4201 aaagctacca ctttaaccac tgcttcggtg gacgacgctg cagtcatcga cgatctcatt
     4261 gctaagatcg accagtgtag gcataacttg tcgccagact tcaggatgaa acctattcag
     4321 ttcgaaaagg atgatgatac aaactatcac atggatgtga tagcaggact agccaacatg
     4381 agggcgagaa attacagcat ccctgaagtg gacaagctga aagcaaagtt catagcaggg
     4441 agaatcatcc cagccattgc gacttcaaca gccatggcta ctgggctggt ctgcctcgag
     4501 ctgtacaagg tccttgatgg aggacataaa gtggaagctt acagaaacac atttgccaac
     4561 ctggcacttc cactattctc catggctgaa ccacttccac caaaggtggt gaagcaccgt
     4621 gacatggctt ggacagtttg ggacagatgg gttctcaagg gaaacccaac tcttcgtgag
     4681 gtgttgcagt ggctggagga caaagggctt agcgcataca gcatctcttg tggaagctgt
     4741 ctactgttca acagtatgtt cacaaggcac aaggagagga tggacaagaa ggttgtggat
     4801 ctcgctcggg atgttgcgaa agtcgagtta ccgccttatc gcaaccatct tgatgtagtg
     4861 gtggcttgtg aggatgaaga tgacaatgac gtcgatattc ctctcgtctc tatctacttc
     4921 aggtgagctt actattaact ttaagaagga gatataccat gggcagccac caccaccacc
     4981 accacagcgg aatgcagatc tttgttaaga ctctcaccgg aaagacaatc accctagagg
     5041 tggaaagctc cgacaccatc gacaacgtta aggccaagat ccaggataag gagggcattc
     5101 ctccggatca gcagaggctt attttcgccg gcaagcagct agaggatggc cgtacgttgg
     5161 ctgattacaa tatccagaag gaatccaccc tccacttggt cctcaggctc cgtggtggtt
     5221 aagcttttac ttaactttaa gaaggagata taccatgtcc cctatactag gttattggaa
     5281 aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
     5341 agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
     5401 tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
     5461 ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
     5521 tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
     5581 tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
     5641 gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
     5701 ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
     5761 cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
     5821 tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
     5881 gtttggtggt ggcgaccatc ctccaaaatc ggatctggtt ccgcgtggat caatggcggg
     5941 tatcaataga gcgggaccat cgggcgctta ttttgttggc cacacagacc ccgagccagt
     6001 atcggggcaa gcacacggat ccggcagcgg cgccagctcc tcgaacagtc cgcaggttca
     6061 gccgcgaccc tcgaatactc ccccgtcgaa cgcgcccgca ccgccgccaa ccggacgtga
     6121 gaggctttca cgatccacgg cgctgtcgcg ccaaaccagg gagtggctgg agcagggtat
     6181 gcctacagcg gaggatgcca gcgtgcgtcg taggccacag gtgactgccg atgccgcaac
     6241 gccgcgtgca gaggcaagac gcacgccgga ggcaactgcc gatgccagcg caccgcgtag
     6301 aggggcggtt gcacacgcca acagtatcgt tcagcaattg gtcagtgagg gcgctgatat
     6361 ttcgcatact cgtaacatgc tccgcaatgc aatgaatggc gacgcagtcg ctttttctcg
     6421 agtagaacag aacatatttc gccagcattt cccgaacatg cccatgcatg gaatcagccg
     6481 agattcggaa ctcgctatcg agctccgtgg agcgcttcgt cgagcggttc accaacaggc
     6541 ggcgtcagcg ccagtgaggt cgcccacgcc aacaccggcc agccctgcgg catcatcatc
     6601 gggcagcagt cagcgttctt tatttggacg gtttgcccgt ttgatggcgc caaaccaggg
     6661 acggtcgtcg aacactgccg cctctcagac gccggtcgac aggagcccgc cacgcgtcaa
     6721 ccaaagaccc atacgcgtcg acagggctgc gatgcgtaat cgtggcaatg acgaggcgga
     6781 cgccgcgctg cgggggttag tacaacaggg ggtcaattta gagcacctgc gcacggccct
     6841 tgaaagacat gtaatgcagc gcctccctat ccccctcgat ataggcagcg cgttgcagaa
     6901 tgtgggaatt aacccaagta tcgacttggg ggaaagcctt gtgcaacatc ccctgctgaa
     6961 tttgaatgta gcgttgaatc gcatgctggg gctgcgtccc agcgctgaaa gagcgcctcg
     7021 tccagccgtc cccgtggctc ccgcgaccgc ctccaggcga ccggatggta cgcgtgcaac
     7081 acgattgcgg gtgatgccgg agcgggagga ttacgaaaat aatgtggcct atggagtgcg
     7141 cttgcttaac ctgaacccgg gggtgggggt aaggcaggct gttgcggcct ttgtaaccga
     7201 ccgggctgag cggccagcag tggtggctaa tatccgggca gccctggacc ctatcgcgtc
     7261 acaattcagt cagctgcgca caatttcgaa ggccgatgct gaatctgaag agctgggttt
     7321 taaggatgcg gcagatcatc acacggatga cgtgacgcac tgtctttttg gcggagaatt
     7381 gtcgctgagt aatccggatc agcaggtgat cggtttggcg ggtaatccga cggacacgtc
     7441 gcagccttac agccaagagg gaaataagga cctggcgttc atggatatga aaaaacttgc
     7501 ccaattcctc gcaggcaagc ctgagcatcc gatgaccaga gaaacgctta acgccgaaaa
     7561 tatcgccaag tatgctttta gaatagtccc ctgagcttca gattaacttt aagaaggaga
     7621 tataccatgg aacaaaagtt gatctctgaa gaggaccttg agcagaaatt gatctctgag
     7681 gaagatcttg agcagaagct tatctcagag gaggatttgg agcaaaaact tatttctgaa
     7741 gaggacctaa tgggaagcaa gtattccaag gcaacaaatt cgataaatga tgcttctaac
     7801 ttgagttatg gcgttccttt tgaaaattat cgagttcctt ttgtagattt ggaggaagca
     7861 actaacaact ttgatgacaa ttttttcatt ggagagggtg gatttgggaa ggtttacagg
     7921 ggtgttttgc gtgatggaac aaaggtcgcc ctgaaaaagc ataaacctga gtcctcacaa
     7981 ggtattgaag agttcgaaac agaaattgag attctctcat tttgcagcca tccgcatctg
     8041 gtttcattga taggattctg tgatgaaaga aatgagatga ttctaattta tgactacatg
     8101 gagaatggga acctcaagag ccatttgtat ggatcagatc taccctccat gagctgggag
     8161 cagaggctgg agatatgtat cggggcagcc agaggccttc actaccttca taagaatgca
     8221 gttatacatc gtgatgtcaa gtgtacaaac atattgcttg atgagaattt tgtgccaaaa
     8281 attactgatt ttggaatatc taagacaatg cccgagcttg accaaaccca tcttagcaca
     8341 gtagtgagag gaaatatagg ctacattgcc cctgaatatg ctttgtgggg acagctgaca
     8401 gaaaaatctg atgtttattc tttcggtgtt gttttattcg aagttctttg tgctaggcct
     8461 gccttagatc gttcagagat aatgagttta gatgatgaga cgcagaagat gggacagttg
     8521 gaacaaattg tagatcccac tattgcggcc aaaataagac cagagtccct caggatgttt
     8581 ggagaaacag cgatcaaatg cttagctccg tctagtaaaa ataggccatc aatgggtgat
     8641 gtgctgtgga aactggagta tgcactttgt ctccaagagc ctaccattca agatgatcct
     8701 gaatgagctt tgtggctttt aagccagccc cgacacccgc caacacccgc tgacgcgccc
     8761 tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgg gatgagatcc
     8821 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact
     8881 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac
     8941 tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     9001 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     9061 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     9121 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     9181 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     9241 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     9301 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     9361 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     9421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     9481 caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat caaatgaaac
     9541 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     9601 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     9661 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     9721 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagtttatgc
     9781 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     9841 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac gcgatcgctg
     9901 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     9961 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
    10021 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
    10081 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
    10141 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
    10201 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
    10261 tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg ttgaatatgg
    10321 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgaccaa
    10381 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    10441 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
    10501 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
    10561 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
    10621 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
    10681 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
    10741 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
    10801 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
    10861 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
    10921 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
    10981 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
    11041 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
    11101 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
    11161 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
    11221 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca atggtgcact
    11281 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac
    11341 gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
    11401 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgactccggg agctgcatgt
    11461 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag
    11521 cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt
    11581 tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt
    11641 cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac
    11701 cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac
    11761 tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca
    11821 ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc
    11881 agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca
    11941 gactttacga aacacggaaa cccaagacca ttcatgttgt tgctcaggtc gcagacgttt
    12001 tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa
    12061 ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg
    12121 gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag
    12181 tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca
    12241 tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct
    12301 gtcctacgag ttgcatgata aagaacacag tcataagtgc ggcgacgata gtcatgcccc
    12361 gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgagatcccg
    12421 gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc gctttccagt
    12481 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    12541 tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg acacgggcaa cagctgattg
    12601 cccttcaccg cctggccctg agagagttgc a
//
