LOCUS       sequence-205471-        9642 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9642)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-296
FEATURES             Location/Qualifiers
     source          1..9642
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     948..967
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(987..1064)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1270..1289
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1373..1392
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1373..1391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1392..1416
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1470..1487
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5035..5124
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5137..5226
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             complement(5744..5761)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5949..5967)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5963..6010
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6047..6502
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6134..6153)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6344..6365
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6594..7409)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6594..7409)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7317..7336
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7401..7420
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7531..8119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8020..8039
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8273..8290
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8305..8444
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8370..8389)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8530..8552
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8546..8737)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9546..9642,1..986))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtggtttttc ttttcaccag tgacacgggc aacagctgat tgcccttcac cgcctggccc
       61 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
      121 atggtggtta acggcgggat ataacatgag ctgtcctcgg tatcgtcgta tcccactacc
      181 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
      241 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
      301 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
      361 tgattgcgag tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt
      421 aatgggcccg ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc
      481 agtcgcgtac cgtcctcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca
      541 tcaagaaata acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca
      601 tccagcggat agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc
      661 gctttacagg cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt
      721 tgatcggcgc gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg
      781 gaggtggcaa cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg
      841 ggaatgtaat tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg
      901 tggctggcct ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg
      961 acatcgtata acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc
     1021 tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc
     1081 tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac
     1141 cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg
     1201 gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc
     1261 ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat
     1321 gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga
     1381 ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat tttgccttaa
     1441 ctttaagaag gagatatacc atgggcagcc accaccacca ccaccacagc ggaatgcaga
     1501 tctttgttaa gactctcacc ggaaagacaa tcaccctaga ggtggaaagc tccgacacca
     1561 tcgacaacgt taaggccaag atccaggata aggagggcat tcctccggat cagcagaggc
     1621 ttattttcgc cggcaagcag ctagaggatg gccgtacgtt ggctgattac aatatccaga
     1681 aggaatccac cctccacttg gtcctcaggc tccgtggtgg ttaagcttgc aattaacttt
     1741 aagaaggaga tatacaatgc ttcacaagcg agctagtgaa gctaacgata agaacgataa
     1801 taccatcatc ggttccgatt tagcatcatc taagaaacgc cgtatcgatt tcactgaatc
     1861 ttcgtctgat aagtcatctt cgattttagc ttctggtagt agcaggggtt ttcacggcga
     1921 tagcgtcgtt cagcaaatcg acatggcttt tggtaattcg aaccgtcagg agattgatga
     1981 agatctgcac agtcgccagc tcgccgtcta tggtcgtgag actatgaggc gtctctttgc
     2041 ttccaatgtt ctcatctcgg ggatgcacgg tcttggtgcc gagattgcca agaatcttat
     2101 acttgctggt gtgaagtctg tgaccttgca tgatgaaaga gttgtagagc tatgggactt
     2161 atcaagcaac tttgttttct cggaggatga tgttggcaag aatagagcag atgcttctgt
     2221 tcagaagtta caggatctta acaatgctgt ggttgtctct agcttgacca aaagcttaaa
     2281 caaagaggat ctttctggct tccaggttgt tgtattctct gacataagca tggaaagagc
     2341 aatcgaattt gatgactatt gtcacagcca tcagcctcct atcgcttttg tcaaggctga
     2401 tgtcaggggt cttttcggct ctgtcttttg tgattttggg cctgaatttg cggttcttga
     2461 tgttgatgga gaggaacccc atacaggcat cattgcgtct atctctaatg agaaccaggc
     2521 atttatttcc tgtgtcgacg atgagaggct tgagtttgaa gatggtgacc tagttgtttt
     2581 ctcagaagtt gagggtatga cagaattgaa tgatgggaaa ccaaggaaga taaaaagtac
     2641 acggccatat tcattcaccc ttgatgagga cactactaac tatggaacat atgtgaaggg
     2701 tggtattgtc actcaagtga aacagccaaa gttactgaat ttcaagccat tgagggaagc
     2761 gcttaaggat ccaggggatt ttctatttag tgatttctct aagtttgatc ggcctccact
     2821 ccttcactta gcattccagg cacttgatca ttttaaagct gaagctggca gatttcctgt
     2881 tgctggctca gaagaggacg ctcagaagct tatatcgatt gccacagcca tcaataccgg
     2941 gcagggtgat ttaaaggtgg agaatgttga tcagaagctt ctaagacact tttcctttgg
     3001 agccaaggct gtcctgaatc ctatggctgc aatgtttggc ggtattgttg gacaggaggt
     3061 tgttaaagct tgctctggaa aatttcatcc tctcttccag tttttctact ttgattcagt
     3121 ggagtcactc ccctctgaac ctgtggactc gagtgacttt gcaccaagaa acagccggta
     3181 tgatgcccaa atatctgtat ttggggccaa gttccagaag aaacttgaag atgctaaagt
     3241 tttcacagta gggtctggtg ctcttggctg tgagttcttg aaaaatttgg ctctgatggg
     3301 ggtttcttgt ggaagccaag ggaagctgac agtgacagat gatgatatca ttgagaagag
     3361 taacctcagt cgtcaatttc tgttccgtga ctggaacatt ggacaggcta aatccacagt
     3421 tgctgcttcg gctgctgctg ttataaatcc tcgcttcaac attgaggccc tgcaaaaccg
     3481 tgttggcgcg gagactgaga atgtatttga cgatgccttc tgggagaact taactgttgt
     3541 cgtcaatgca ctggataatg ttaatgcgag gctctatgtt gattcgaggt gcttgtattt
     3601 ccagaagcct ctccttgagt ctggaactct tggtacaaag tgcaacacac agtcggttat
     3661 cccacatcta actgaaaatt acggtgcctc aagggaccca ccagagaaac aggcccccat
     3721 gtgtacagtg cattccttcc cgcataacat tgatcactgt ttaacttggg ctcgctctga
     3781 gtttgagggt ctgctggaga aaactcccgc tgaagtgaat gcatatcttt ctagcccagt
     3841 tgagtacact aactcaatga tgagtgctgg tgatgctcag gcgagggaca cattggagag
     3901 gattgttgag tgccttgaaa aggagaagtg tgaaaccttc caagactgct taacctgggc
     3961 acgactgagg tttgaggatt actttgtgaa ccgtgtcaag caattgatat acacatttcc
     4021 tgaagatgct gcgactagta ccggagctcc attctggtct gctccaaaaa gattccctcg
     4081 tccactccag tactcgtcgt ccgacccaag tctccttaac tttatcacag cgactgctat
     4141 tttacgagca gagacatttg ggatcccgat acctgagtgg actaagaacc caaaggaagc
     4201 agcagaagct gttgacagag tgatagtccc agactttgag ccaaggcaag atgcaaagat
     4261 tgtgactgat gagaaagcta ccactttaac cactgcttcg gtggacgacg ctgcagtcat
     4321 cgacgatctc attgctaaga tcgaccagtg taggcataac ttgtcgccag acttcaggat
     4381 gaaacctatt cagttcgaaa aggatgatga tacaaactat cacatggatg tgatagcagg
     4441 actagccaac atgagggcga gaaattacag catccctgaa gtggacaagc tgaaagcaaa
     4501 gttcatagca gggagaatca tcccagccat tgcgacttca acagccatgg ctactgggct
     4561 ggtctgcctc gagctgtaca aggtccttga tggaggacat aaagtggaag cttacagaaa
     4621 cacatttgcc aacctggcac ttccactatt ctccatggct gaaccacttc caccaaaggt
     4681 ggtgaagcac cgtgacatgg cttggacagt ttgggacaga tgggttctca agggaaaccc
     4741 aactcttcgt gaggtgttgc agtggctgga ggacaaaggg cttagcgcat acagcatctc
     4801 ttgtggaagc tgtctactgt tcaacagtat gttcacaagg cacaaggaga ggatggacaa
     4861 gaaggttgtg gatctcgctc gggatgttgc gaaagtcgag ttaccgcctt atcgcaacca
     4921 tcttgatgta gtggtggctt gtgaggatga agatgacaat gacgtcgata ttcctctcgt
     4981 ctctatctac ttcaggtgag cttactatta actttaagaa ggagatatac catgtaccca
     5041 tacgatgttc ctgactatgc gggctatccc tatgacgtcc cggactatgc aggattgtat
     5101 ccatatgacg ttccagatta cgccactaga gctgcttacc catacgatgt tcctgactat
     5161 gcgggctatc cctatgacgt cccggactat gcaggattgt atccatatga cgttccagat
     5221 tacgcaatgt catcgccaag caaacgcaga gaaatggata tgatgaagct gatgatgagc
     5281 gattataaag tggaaacgat caacgatggc atgcaagaat tctatgttga attcaatggt
     5341 cccaaagaca gtctctatca aggaggtgtg tggaagataa gagttgagct tccagatgct
     5401 tatccttata aatctccatc tgttggtttc attactaaaa tttatcatcc taatgttgat
     5461 gaactgtcgg gttctgtttg tttagatgtg attaaccaaa cttggagtcc tatgttcgac
     5521 cttgtgaatg tgtttgagac atttcttcct cagcttcttc tgtatccaaa cccatcagat
     5581 ccattgaatg gagaagctgc tgcgttaatg atgcgtgatc gtcctgctta tgagcaacga
     5641 gttaaagaat actgtgagaa gtatgcaaag ccaggggaag gttcagaaga taagtctagc
     5701 gatgaagaac taagtgaaga ggaatacggc tcagataatg aggatgatga tgatgatgat
     5761 gttgcaattg ctggcaaacc agatccttga gcttttacgc ttttaagcca gccccgacac
     5821 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     5881 caagctgtga ccgggatgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc
     5941 tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag
     6001 gggttttttg ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc
     6061 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6121 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6181 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     6241 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     6301 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     6361 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     6421 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     6481 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa
     6601 aactcatcga gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat
     6661 ttttgaaaaa gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg
     6721 gcaagatcct ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat
     6781 ttcccctcgt caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc
     6841 ggtgagaatg gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta
     6901 cgctcgtcat caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga
     6961 gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac
     7021 cggcgcagga acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct
     7081 aatacctgga atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga
     7141 gtacggataa aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg
     7201 accatctcat ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct
     7261 ggcgcatcgg gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg
     7321 cgagcccatt tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag
     7381 caagacgttt cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca
     7441 gacagtttta ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7501 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7561 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     7681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     8161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     8221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     8281 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     8341 tatttcacac cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     8401 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     8461 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     8521 tgaccgactc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     8581 ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt
     8641 catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc
     8701 gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat
     8761 ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta
     8821 ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga
     8881 tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg
     8941 taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc
     9001 agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaacccaag accattcatg
     9061 ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg
     9121 gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca
     9181 ggagcacgat catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc
     9241 cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga
     9301 ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa
     9361 tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaac acagtcataa
     9421 gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc
     9481 tcaagggcat cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt
     9541 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     9601 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgcca gg
//
