LOCUS       sequence-205472-        9531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-295
FEATURES             Location/Qualifiers
     source          1..9531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     961..980
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1000..1077)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1283..1302
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1386..1405
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1386..1404
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1405..1429
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1483..1500
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5048..5137
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5150..5239
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5851..5869)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5865..5912
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5949..6404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6036..6055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6246..6267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6496..7311)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6496..7311)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7219..7238
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7303..7322
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7433..8021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7922..7941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8175..8192
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8207..8346
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8272..8291)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8432..8454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8448..8639)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9448..9531,1..999))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 attgggcgcc agggtggttt ttcttttcac cagtgacacg ggcaacagct gattgccctt
       61 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
      121 aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtcct cggtatcgtc
      181 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
      241 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
      301 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
      361 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
      421 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
      481 atgctccacg cccagtcgcg taccgtcctc atgggagaaa ataatactgt tgatgggtgt
      541 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
      601 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
      661 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
      721 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
      781 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
      841 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
      901 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
      961 ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact
     1021 ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg
     1081 gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     1141 ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     1201 ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     1261 ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     1321 tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc
     1381 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat
     1441 aattttgcct taactttaag aaggagatat accatgggca gccaccacca ccaccaccac
     1501 agcggaatgc agatctttgt taagactctc accggaaaga caatcaccct agaggtggaa
     1561 agctccgaca ccatcgacaa cgttaaggcc aagatccagg ataaggaggg cattcctccg
     1621 gatcagcaga ggcttatttt cgccggcaag cagctagagg atggccgtac gttggctgat
     1681 tacaatatcc agaaggaatc caccctccac ttggtcctca ggctccgtgg tggttaagct
     1741 tgcaattaac tttaagaagg agatatacaa tgcttcacaa gcgagctagt gaagctaacg
     1801 ataagaacga taataccatc atcggttccg atttagcatc atctaagaaa cgccgtatcg
     1861 atttcactga atcttcgtct gataagtcat cttcgatttt agcttctggt agtagcaggg
     1921 gttttcacgg cgatagcgtc gttcagcaaa tcgacatggc ttttggtaat tcgaaccgtc
     1981 aggagattga tgaagatctg cacagtcgcc agctcgccgt ctatggtcgt gagactatga
     2041 ggcgtctctt tgcttccaat gttctcatct cggggatgca cggtcttggt gccgagattg
     2101 ccaagaatct tatacttgct ggtgtgaagt ctgtgacctt gcatgatgaa agagttgtag
     2161 agctatggga cttatcaagc aactttgttt tctcggagga tgatgttggc aagaatagag
     2221 cagatgcttc tgttcagaag ttacaggatc ttaacaatgc tgtggttgtc tctagcttga
     2281 ccaaaagctt aaacaaagag gatctttctg gcttccaggt tgttgtattc tctgacataa
     2341 gcatggaaag agcaatcgaa tttgatgact attgtcacag ccatcagcct cctatcgctt
     2401 ttgtcaaggc tgatgtcagg ggtcttttcg gctctgtctt ttgtgatttt gggcctgaat
     2461 ttgcggttct tgatgttgat ggagaggaac cccatacagg catcattgcg tctatctcta
     2521 atgagaacca ggcatttatt tcctgtgtcg acgatgagag gcttgagttt gaagatggtg
     2581 acctagttgt tttctcagaa gttgagggta tgacagaatt gaatgatggg aaaccaagga
     2641 agataaaaag tacacggcca tattcattca cccttgatga ggacactact aactatggaa
     2701 catatgtgaa gggtggtatt gtcactcaag tgaaacagcc aaagttactg aatttcaagc
     2761 cattgaggga agcgcttaag gatccagggg attttctatt tagtgatttc tctaagtttg
     2821 atcggcctcc actccttcac ttagcattcc aggcacttga tcattttaaa gctgaagctg
     2881 gcagatttcc tgttgctggc tcagaagagg acgctcagaa gcttatatcg attgccacag
     2941 ccatcaatac cgggcagggt gatttaaagg tggagaatgt tgatcagaag cttctaagac
     3001 acttttcctt tggagccaag gctgtcctga atcctatggc tgcaatgttt ggcggtattg
     3061 ttggacagga ggttgttaaa gcttgctctg gaaaatttca tcctctcttc cagtttttct
     3121 actttgattc agtggagtca ctcccctctg aacctgtgga ctcgagtgac tttgcaccaa
     3181 gaaacagccg gtatgatgcc caaatatctg tatttggggc caagttccag aagaaacttg
     3241 aagatgctaa agttttcaca gtagggtctg gtgctcttgg ctgtgagttc ttgaaaaatt
     3301 tggctctgat gggggtttct tgtggaagcc aagggaagct gacagtgaca gatgatgata
     3361 tcattgagaa gagtaacctc agtcgtcaat ttctgttccg tgactggaac attggacagg
     3421 ctaaatccac agttgctgct tcggctgctg ctgttataaa tcctcgcttc aacattgagg
     3481 ccctgcaaaa ccgtgttggc gcggagactg agaatgtatt tgacgatgcc ttctgggaga
     3541 acttaactgt tgtcgtcaat gcactggata atgttaatgc gaggctctat gttgattcga
     3601 ggtgcttgta tttccagaag cctctccttg agtctggaac tcttggtaca aagtgcaaca
     3661 cacagtcggt tatcccacat ctaactgaaa attacggtgc ctcaagggac ccaccagaga
     3721 aacaggcccc catgtgtaca gtgcattcct tcccgcataa cattgatcac tgtttaactt
     3781 gggctcgctc tgagtttgag ggtctgctgg agaaaactcc cgctgaagtg aatgcatatc
     3841 tttctagccc agttgagtac actaactcaa tgatgagtgc tggtgatgct caggcgaggg
     3901 acacattgga gaggattgtt gagtgccttg aaaaggagaa gtgtgaaacc ttccaagact
     3961 gcttaacctg ggcacgactg aggtttgagg attactttgt gaaccgtgtc aagcaattga
     4021 tatacacatt tcctgaagat gctgcgacta gtaccggagc tccattctgg tctgctccaa
     4081 aaagattccc tcgtccactc cagtactcgt cgtccgaccc aagtctcctt aactttatca
     4141 cagcgactgc tattttacga gcagagacat ttgggatccc gatacctgag tggactaaga
     4201 acccaaagga agcagcagaa gctgttgaca gagtgatagt cccagacttt gagccaaggc
     4261 aagatgcaaa gattgtgact gatgagaaag ctaccacttt aaccactgct tcggtggacg
     4321 acgctgcagt catcgacgat ctcattgcta agatcgacca gtgtaggcat aacttgtcgc
     4381 cagacttcag gatgaaacct attcagttcg aaaaggatga tgatacaaac tatcacatgg
     4441 atgtgatagc aggactagcc aacatgaggg cgagaaatta cagcatccct gaagtggaca
     4501 agctgaaagc aaagttcata gcagggagaa tcatcccagc cattgcgact tcaacagcca
     4561 tggctactgg gctggtctgc ctcgagctgt acaaggtcct tgatggagga cataaagtgg
     4621 aagcttacag aaacacattt gccaacctgg cacttccact attctccatg gctgaaccac
     4681 ttccaccaaa ggtggtgaag caccgtgaca tggcttggac agtttgggac agatgggttc
     4741 tcaagggaaa cccaactctt cgtgaggtgt tgcagtggct ggaggacaaa gggcttagcg
     4801 catacagcat ctcttgtgga agctgtctac tgttcaacag tatgttcaca aggcacaagg
     4861 agaggatgga caagaaggtt gtggatctcg ctcgggatgt tgcgaaagtc gagttaccgc
     4921 cttatcgcaa ccatcttgat gtagtggtgg cttgtgagga tgaagatgac aatgacgtcg
     4981 atattcctct cgtctctatc tacttcaggt gagcttacta ttaactttaa gaaggagata
     5041 taccatgtac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     5101 tgcaggattg tatccatatg acgttccaga ttacgccact agagctgctt acccatacga
     5161 tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat tgtatccata
     5221 tgacgttcca gattacgcaa tgacaactcc agcaaaaaag agactgatgt gggatttcaa
     5281 gagactgcag aaagatcctc ctgtgggaat aagtggagct ccacaagata acaatatcat
     5341 gcattggaat gctctcatct ttggacctga ggacactcca tgggatggag gtactttcaa
     5401 gttgactctt cacttcaccg aggattatcc gaacaaaccc ccgattgttc ggtttgtttc
     5461 acggatgttt catccaaaca tttatgctga tggaagcatt tgcttggata tcttgcaaaa
     5521 ccagtggagc cccatctacg acgttgctgc agttctcaca tcaattcagt ctttgctctg
     5581 tgacccaaac cctgactcac cagcgaacgc agaggctgca aggttgttca gtgagaataa
     5641 aagagaatat aacaggaaag taatcgagat cgtggagcag agctatgtat aagctttnac
     5701 gcttttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     5761 gctcccggca tccgcttaca gacaagctgt gaccgggatg agatccggct gctaacaaag
     5821 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg
     5881 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggattgg
     5941 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6001 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6061 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6121 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6181 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6241 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6301 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6361 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6421 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6481 ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca atttattcat
     6541 atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag gagaaaactc
     6601 accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc
     6661 aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa gtgagaaatc
     6721 accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt ctttccagac
     6781 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt
     6841 attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt
     6901 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc
     6961 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt
     7021 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa
     7081 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt
     7141 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc
     7201 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt
     7261 ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca taacacccct
     7321 tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc ccttaacgtg
     7381 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7441 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7501 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7561 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7621 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7681 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7741 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7801 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7861 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7921 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7981 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8041 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8101 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8161 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     8221 ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg
     8281 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     8341 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     8401 gcatccgctt acagacaagc tgtgaccgac tccgggagct gcatgtgtca gaggttttca
     8461 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     8521 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     8581 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     8641 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     8701 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
     8761 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
     8821 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
     8881 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
     8941 cggaaaccca agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
     9001 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
     9061 cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc cgccatgccg
     9121 gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga
     9181 gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag
     9241 cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc
     9301 atgataaaga acacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag
     9361 gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc ctaatgagtg
     9421 agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     9481 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg t
//
