LOCUS       sequence-205473-        9636 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9636)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9636)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-297
FEATURES             Location/Qualifiers
     source          1..9636
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     84..103
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        84..102
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    103..127
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             181..198
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3746..3835
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3848..3937
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4654..4672)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4668..4715
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4752..5207
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4839..4858)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5049..5070
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5299..6114)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5299..6114)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6022..6041
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6106..6125
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6236..6824
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6725..6744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6978..6995
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7010..7149
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7075..7094)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7235..7257
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7251..7442)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8251..9333)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9295..9314
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9334..9411)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9617..9636
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga
       61 tcgagatctc gatcccgcga aattaatacg actcactata ggggaattgt gagcggataa
      121 caattcccct ctagaaataa ttttgcctta actttaagaa ggagatatac catgggcagc
      181 caccaccacc accaccacag cggaatgcag atctttgtta agactctcac cggaaagaca
      241 atcaccctag aggtggaaag ctccgacacc atcgacaacg ttaaggccaa gatccaggat
      301 aaggagggca ttcctccgga tcagcagagg cttattttcg ccggcaagca gctagaggat
      361 ggccgtacgt tggctgatta caatatccag aaggaatcca ccctccactt ggtcctcagg
      421 ctccgtggtg gttaagcttg caattaactt taagaaggag atatacaatg cttcacaagc
      481 gagctagtga agctaacgat aagaacgata ataccatcat cggttccgat ttagcatcat
      541 ctaagaaacg ccgtatcgat ttcactgaat cttcgtctga taagtcatct tcgattttag
      601 cttctggtag tagcaggggt tttcacggcg atagcgtcgt tcagcaaatc gacatggctt
      661 ttggtaattc gaaccgtcag gagattgatg aagatctgca cagtcgccag ctcgccgtct
      721 atggtcgtga gactatgagg cgtctctttg cttccaatgt tctcatctcg gggatgcacg
      781 gtcttggtgc cgagattgcc aagaatctta tacttgctgg tgtgaagtct gtgaccttgc
      841 atgatgaaag agttgtagag ctatgggact tatcaagcaa ctttgttttc tcggaggatg
      901 atgttggcaa gaatagagca gatgcttctg ttcagaagtt acaggatctt aacaatgctg
      961 tggttgtctc tagcttgacc aaaagcttaa acaaagagga tctttctggc ttccaggttg
     1021 ttgtattctc tgacataagc atggaaagag caatcgaatt tgatgactat tgtcacagcc
     1081 atcagcctcc tatcgctttt gtcaaggctg atgtcagggg tcttttcggc tctgtctttt
     1141 gtgattttgg gcctgaattt gcggttcttg atgttgatgg agaggaaccc catacaggca
     1201 tcattgcgtc tatctctaat gagaaccagg catttatttc ctgtgtcgac gatgagaggc
     1261 ttgagtttga agatggtgac ctagttgttt tctcagaagt tgagggtatg acagaattga
     1321 atgatgggaa accaaggaag ataaaaagta cacggccata ttcattcacc cttgatgagg
     1381 acactactaa ctatggaaca tatgtgaagg gtggtattgt cactcaagtg aaacagccaa
     1441 agttactgaa tttcaagcca ttgagggaag cgcttaagga tccaggggat tttctattta
     1501 gtgatttctc taagtttgat cggcctccac tccttcactt agcattccag gcacttgatc
     1561 attttaaagc tgaagctggc agatttcctg ttgctggctc agaagaggac gctcagaagc
     1621 ttatatcgat tgccacagcc atcaataccg ggcagggtga tttaaaggtg gagaatgttg
     1681 atcagaagct tctaagacac ttttcctttg gagccaaggc tgtcctgaat cctatggctg
     1741 caatgtttgg cggtattgtt ggacaggagg ttgttaaagc ttgctctgga aaatttcatc
     1801 ctctcttcca gtttttctac tttgattcag tggagtcact cccctctgaa cctgtggact
     1861 cgagtgactt tgcaccaaga aacagccggt atgatgccca aatatctgta tttggggcca
     1921 agttccagaa gaaacttgaa gatgctaaag ttttcacagt agggtctggt gctcttggct
     1981 gtgagttctt gaaaaatttg gctctgatgg gggtttcttg tggaagccaa gggaagctga
     2041 cagtgacaga tgatgatatc attgagaaga gtaacctcag tcgtcaattt ctgttccgtg
     2101 actggaacat tggacaggct aaatccacag ttgctgcttc ggctgctgct gttataaatc
     2161 ctcgcttcaa cattgaggcc ctgcaaaacc gtgttggcgc ggagactgag aatgtatttg
     2221 acgatgcctt ctgggagaac ttaactgttg tcgtcaatgc actggataat gttaatgcga
     2281 ggctctatgt tgattcgagg tgcttgtatt tccagaagcc tctccttgag tctggaactc
     2341 ttggtacaaa gtgcaacaca cagtcggtta tcccacatct aactgaaaat tacggtgcct
     2401 caagggaccc accagagaaa caggccccca tgtgtacagt gcattccttc ccgcataaca
     2461 ttgatcactg tttaacttgg gctcgctctg agtttgaggg tctgctggag aaaactcccg
     2521 ctgaagtgaa tgcatatctt tctagcccag ttgagtacac taactcaatg atgagtgctg
     2581 gtgatgctca ggcgagggac acattggaga ggattgttga gtgccttgaa aaggagaagt
     2641 gtgaaacctt ccaagactgc ttaacctggg cacgactgag gtttgaggat tactttgtga
     2701 accgtgtcaa gcaattgata tacacatttc ctgaagatgc tgcgactagt accggagctc
     2761 cattctggtc tgctccaaaa agattccctc gtccactcca gtactcgtcg tccgacccaa
     2821 gtctccttaa ctttatcaca gcgactgcta ttttacgagc agagacattt gggatcccga
     2881 tacctgagtg gactaagaac ccaaaggaag cagcagaagc tgttgacaga gtgatagtcc
     2941 cagactttga gccaaggcaa gatgcaaaga ttgtgactga tgagaaagct accactttaa
     3001 ccactgcttc ggtggacgac gctgcagtca tcgacgatct cattgctaag atcgaccagt
     3061 gtaggcataa cttgtcgcca gacttcagga tgaaacctat tcagttcgaa aaggatgatg
     3121 atacaaacta tcacatggat gtgatagcag gactagccaa catgagggcg agaaattaca
     3181 gcatccctga agtggacaag ctgaaagcaa agttcatagc agggagaatc atcccagcca
     3241 ttgcgacttc aacagccatg gctactgggc tggtctgcct cgagctgtac aaggtccttg
     3301 atggaggaca taaagtggaa gcttacagaa acacatttgc caacctggca cttccactat
     3361 tctccatggc tgaaccactt ccaccaaagg tggtgaagca ccgtgacatg gcttggacag
     3421 tttgggacag atgggttctc aagggaaacc caactcttcg tgaggtgttg cagtggctgg
     3481 aggacaaagg gcttagcgca tacagcatct cttgtggaag ctgtctactg ttcaacagta
     3541 tgttcacaag gcacaaggag aggatggaca agaaggttgt ggatctcgct cgggatgttg
     3601 cgaaagtcga gttaccgcct tatcgcaacc atcttgatgt agtggtggct tgtgaggatg
     3661 aagatgacaa tgacgtcgat attcctctcg tctctatcta cttcaggtga gcttactatt
     3721 aactttaaga aggagatata ccatgtaccc atacgatgtt cctgactatg cgggctatcc
     3781 ctatgacgtc ccggactatg caggattgta tccatatgac gttccagatt acgccactag
     3841 agctgcttac ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta
     3901 tgcaggattg tatccatatg acgttccaga ttacgcaatg tcatcgccga gcaagcgtag
     3961 agaaatggat ttgatgaaac tgatgatgag tgactacaag gtggagatga tcaatgatgg
     4021 catgcaagag ttctttgtcg aattcagtgg acccaaagac agtatatatg agggaggtgt
     4081 gtggaagata agagttgaac ttcctgatgc ttatccttat aaatctccat ctgttggttt
     4141 cattaccaaa atataccacc ctaatgtcga tgaaatgtca ggttctgttt gtctagatgt
     4201 tattaaccag acctggagcc cgatgttcga cctggtgaat gtgttcgaga catttcttcc
     4261 gcaacttctt ctgtatccga atccgtcaga tccattgaat ggtgaagcag ctgcattgat
     4321 gatgcgtgat cgtcctacct atgaacagag agttaaagag tattgtgaga aatacgcaaa
     4381 gccaagggcg gatactgagg aaatgtctag tgatgatgaa atgagtgaag atgaatatgc
     4441 ctcggatggt gatgatgaag atgatgttgc aatcgccggt aaacttgatc cttaagcttt
     4501 tacgctttta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4561 tctgctcccg gcatccgctt acagacaagc tgtgaccggg atgagatccg gctgctaaca
     4621 aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc
     4681 ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat
     4741 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4801 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4861 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4921 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4981 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     5041 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     5101 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     5161 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
     5221 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     5281 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
     5341 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
     5401 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
     5461 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
     5521 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
     5581 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
     5641 gttattcatt cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca
     5701 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     5761 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     5821 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     5881 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     5941 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     6001 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     6061 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     6121 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     6181 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6241 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6301 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6361 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     6421 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6481 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6541 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6601 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6661 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6721 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     6781 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     6841 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     6901 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     6961 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     7021 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     7081 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     7141 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     7201 ccggcatccg cttacagaca agctgtgacc gactccggga gctgcatgtg tcagaggttt
     7261 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     7321 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     7381 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     7441 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     7501 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     7561 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     7621 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     7681 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     7741 acacggaaac ccaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     7801 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     7861 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     7921 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     7981 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     8041 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     8101 tgcatgataa agaacacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     8161 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     8221 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8281 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8341 cgccagggtg gtttttcttt tcaccagtga cacgggcaac agctgattgc ccttcaccgc
     8401 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     8461 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcctcggtat cgtcgtatcc
     8521 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     8581 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     8641 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     8701 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     8761 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     8821 cacgcccagt cgcgtaccgt cctcatggga gaaaataata ctgttgatgg gtgtctggtc
     8881 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     8941 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     9001 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     9061 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     9121 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     9181 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     9241 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     9301 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     9361 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     9421 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     9481 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     9541 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     9601 cccgatcttc cccatcggtg atgtcggcga tatagg
//
