LOCUS       sequence-205474-        9630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9630)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-298
FEATURES             Location/Qualifiers
     source          1..9630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     555..574
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(594..671)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     877..896
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     980..999
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        980..998
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    999..1023
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1077..1094
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4642..4731
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4744..4833
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             complement(5294..5311)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5544..5562)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5558..5605
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5642..6097
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5729..5748)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5939..5960
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6189..7004)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6189..7004)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6912..6931
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6996..7015
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7126..7714
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7615..7634
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7868..7885
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7900..8039
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7965..7984)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8125..8147
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8141..8332)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9141..9630,1..593))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga
       61 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cctcatggga gaaaataata
      121 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca
      181 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg
      241 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc
      301 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt
      361 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg
      421 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc
      481 actttttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc
      541 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc
      601 accctgaatt gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat
      661 tcgatggtgt ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc
      721 cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat
      781 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
      841 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
      901 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga
      961 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
     1021 tcccctctag aaataatttt gccttaactt taagaaggag atataccatg ggcagccacc
     1081 accaccacca ccacagcgga atgcagatct ttgttaagac tctcaccgga aagacaatca
     1141 ccctagaggt ggaaagctcc gacaccatcg acaacgttaa ggccaagatc caggataagg
     1201 agggcattcc tccggatcag cagaggctta ttttcgccgg caagcagcta gaggatggcc
     1261 gtacgttggc tgattacaat atccagaagg aatccaccct ccacttggtc ctcaggctcc
     1321 gtggtggtta agcttgcaat taactttaag aaggagatat acaatgcttc acaagcgagc
     1381 tagtgaagct aacgataaga acgataatac catcatcggt tccgatttag catcatctaa
     1441 gaaacgccgt atcgatttca ctgaatcttc gtctgataag tcatcttcga ttttagcttc
     1501 tggtagtagc aggggttttc acggcgatag cgtcgttcag caaatcgaca tggcttttgg
     1561 taattcgaac cgtcaggaga ttgatgaaga tctgcacagt cgccagctcg ccgtctatgg
     1621 tcgtgagact atgaggcgtc tctttgcttc caatgttctc atctcgggga tgcacggtct
     1681 tggtgccgag attgccaaga atcttatact tgctggtgtg aagtctgtga ccttgcatga
     1741 tgaaagagtt gtagagctat gggacttatc aagcaacttt gttttctcgg aggatgatgt
     1801 tggcaagaat agagcagatg cttctgttca gaagttacag gatcttaaca atgctgtggt
     1861 tgtctctagc ttgaccaaaa gcttaaacaa agaggatctt tctggcttcc aggttgttgt
     1921 attctctgac ataagcatgg aaagagcaat cgaatttgat gactattgtc acagccatca
     1981 gcctcctatc gcttttgtca aggctgatgt caggggtctt ttcggctctg tcttttgtga
     2041 ttttgggcct gaatttgcgg ttcttgatgt tgatggagag gaaccccata caggcatcat
     2101 tgcgtctatc tctaatgaga accaggcatt tatttcctgt gtcgacgatg agaggcttga
     2161 gtttgaagat ggtgacctag ttgttttctc agaagttgag ggtatgacag aattgaatga
     2221 tgggaaacca aggaagataa aaagtacacg gccatattca ttcacccttg atgaggacac
     2281 tactaactat ggaacatatg tgaagggtgg tattgtcact caagtgaaac agccaaagtt
     2341 actgaatttc aagccattga gggaagcgct taaggatcca ggggattttc tatttagtga
     2401 tttctctaag tttgatcggc ctccactcct tcacttagca ttccaggcac ttgatcattt
     2461 taaagctgaa gctggcagat ttcctgttgc tggctcagaa gaggacgctc agaagcttat
     2521 atcgattgcc acagccatca ataccgggca gggtgattta aaggtggaga atgttgatca
     2581 gaagcttcta agacactttt cctttggagc caaggctgtc ctgaatccta tggctgcaat
     2641 gtttggcggt attgttggac aggaggttgt taaagcttgc tctggaaaat ttcatcctct
     2701 cttccagttt ttctactttg attcagtgga gtcactcccc tctgaacctg tggactcgag
     2761 tgactttgca ccaagaaaca gccggtatga tgcccaaata tctgtatttg gggccaagtt
     2821 ccagaagaaa cttgaagatg ctaaagtttt cacagtaggg tctggtgctc ttggctgtga
     2881 gttcttgaaa aatttggctc tgatgggggt ttcttgtgga agccaaggga agctgacagt
     2941 gacagatgat gatatcattg agaagagtaa cctcagtcgt caatttctgt tccgtgactg
     3001 gaacattgga caggctaaat ccacagttgc tgcttcggct gctgctgtta taaatcctcg
     3061 cttcaacatt gaggccctgc aaaaccgtgt tggcgcggag actgagaatg tatttgacga
     3121 tgccttctgg gagaacttaa ctgttgtcgt caatgcactg gataatgtta atgcgaggct
     3181 ctatgttgat tcgaggtgct tgtatttcca gaagcctctc cttgagtctg gaactcttgg
     3241 tacaaagtgc aacacacagt cggttatccc acatctaact gaaaattacg gtgcctcaag
     3301 ggacccacca gagaaacagg cccccatgtg tacagtgcat tccttcccgc ataacattga
     3361 tcactgttta acttgggctc gctctgagtt tgagggtctg ctggagaaaa ctcccgctga
     3421 agtgaatgca tatctttcta gcccagttga gtacactaac tcaatgatga gtgctggtga
     3481 tgctcaggcg agggacacat tggagaggat tgttgagtgc cttgaaaagg agaagtgtga
     3541 aaccttccaa gactgcttaa cctgggcacg actgaggttt gaggattact ttgtgaaccg
     3601 tgtcaagcaa ttgatataca catttcctga agatgctgcg actagtaccg gagctccatt
     3661 ctggtctgct ccaaaaagat tccctcgtcc actccagtac tcgtcgtccg acccaagtct
     3721 ccttaacttt atcacagcga ctgctatttt acgagcagag acatttggga tcccgatacc
     3781 tgagtggact aagaacccaa aggaagcagc agaagctgtt gacagagtga tagtcccaga
     3841 ctttgagcca aggcaagatg caaagattgt gactgatgag aaagctacca ctttaaccac
     3901 tgcttcggtg gacgacgctg cagtcatcga cgatctcatt gctaagatcg accagtgtag
     3961 gcataacttg tcgccagact tcaggatgaa acctattcag ttcgaaaagg atgatgatac
     4021 aaactatcac atggatgtga tagcaggact agccaacatg agggcgagaa attacagcat
     4081 ccctgaagtg gacaagctga aagcaaagtt catagcaggg agaatcatcc cagccattgc
     4141 gacttcaaca gccatggcta ctgggctggt ctgcctcgag ctgtacaagg tccttgatgg
     4201 aggacataaa gtggaagctt acagaaacac atttgccaac ctggcacttc cactattctc
     4261 catggctgaa ccacttccac caaaggtggt gaagcaccgt gacatggctt ggacagtttg
     4321 ggacagatgg gttctcaagg gaaacccaac tcttcgtgag gtgttgcagt ggctggagga
     4381 caaagggctt agcgcataca gcatctcttg tggaagctgt ctactgttca acagtatgtt
     4441 cacaaggcac aaggagagga tggacaagaa ggttgtggat ctcgctcggg atgttgcgaa
     4501 agtcgagtta ccgccttatc gcaaccatct tgatgtagtg gtggcttgtg aggatgaaga
     4561 tgacaatgac gtcgatattc ctctcgtctc tatctacttc aggtgagctt actattaact
     4621 ttaagaagga gatataccat gtacccatac gatgttcctg actatgcggg ctatccctat
     4681 gacgtcccgg actatgcagg attgtatcca tatgacgttc cagattacgc cactagagct
     4741 gcttacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca
     4801 ggattgtatc catatgacgt tccagattac gcaatggcgt cgccgagcaa acgtcgagag
     4861 atggatatga tgaaactgat gatgagtgat tacaaggtgg atactgtcaa cgacgatttg
     4921 caaatgttct atgtcacatt ccatggaccc actgacagtc tttatcaggg gggtgtatgg
     4981 aagataaaag tggaacttcc cgaagcttat ccatacaaat ctccatctgt tggatttgtc
     5041 aacaagattt atcaccctaa tgtcgatgaa tcttctggtg ctgtttgctt agatgttata
     5101 aaccagactt ggagcccaat gtttgatctt ataaatgtgt tcgaatcctt ccttccgcaa
     5161 ctgcttctat atccaaaccc atcagatcca tttaatggag aagccgcttc tctgttgatg
     5221 cgtgaccgtg cagcctatga gctcaaagtg aaagaatatt gtgagaaata tgcaaaacca
     5281 gaggagattt taagtgatga tgatgatgat gatagcatga gcgaagatgg ttcggactct
     5341 gatgatgatg acgacgacga aatcgttggg aaagccgatc catgagcttt tacgctttta
     5401 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     5461 gcatccgctt acagacaagc tgtgaccggg atgagatccg gctgctaaca aagcccgaaa
     5521 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     5581 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
     5641 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     5701 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     5761 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     5821 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     5881 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     5941 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     6001 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     6061 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     6121 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     6181 attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     6241 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     6301 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     6361 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     6421 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     6481 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     6541 cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca attacaaaca
     6601 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     6661 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     6721 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     6781 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     6841 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     6901 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     6961 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     7021 ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc
     7081 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7141 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7201 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7261 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7321 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     7381 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     7441 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     7501 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     7561 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     7621 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     7681 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     7741 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     7801 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     7861 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     7921 tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat
     7981 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     8041 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8101 cttacagaca agctgtgacc gactccggga gctgcatgtg tcagaggttt tcaccgtcat
     8161 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
     8221 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
     8281 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
     8341 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
     8401 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
     8461 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
     8521 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
     8581 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
     8641 ccaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
     8701 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
     8761 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa
     8821 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
     8881 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
     8941 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
     9001 agaacacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
     9061 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
     9121 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     9181 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgccagggtg
     9241 gtttttcttt tcaccagtga cacgggcaac agctgattgc ccttcaccgc ctggccctga
     9301 gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc ctgtttgatg
     9361 gtggttaacg gcgggatata acatgagctg tcctcggtat cgtcgtatcc cactaccgag
     9421 atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc cagcgccatc
     9481 tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat ttgcatggtt
     9541 tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg ctgaatttga
     9601 ttgcgagtga gatatttatg ccagccagcc
//
