LOCUS       sequence-205475-        9615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-300
FEATURES             Location/Qualifiers
     source          1..9615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     45..64
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     148..167
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        148..166
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    167..191
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             245..262
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3810..3899
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3912..4001
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4697..4715)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4711..4758
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4795..5250
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4882..4901)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5092..5113
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5342..6157)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5342..6157)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6065..6084
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6149..6168
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6279..6867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6768..6787
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7021..7038
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7053..7192
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7118..7137)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7278..7300
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7294..7485)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8294..9376)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9338..9357
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9377..9454)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
       61 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      121 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
      181 ataacaattc ccctctagaa ataattttgc cttaacttta agaaggagat ataccatggg
      241 cagccaccac caccaccacc acagcggaat gcagatcttt gttaagactc tcaccggaaa
      301 gacaatcacc ctagaggtgg aaagctccga caccatcgac aacgttaagg ccaagatcca
      361 ggataaggag ggcattcctc cggatcagca gaggcttatt ttcgccggca agcagctaga
      421 ggatggccgt acgttggctg attacaatat ccagaaggaa tccaccctcc acttggtcct
      481 caggctccgt ggtggttaag cttgcaatta actttaagaa ggagatatac aatgcttcac
      541 aagcgagcta gtgaagctaa cgataagaac gataatacca tcatcggttc cgatttagca
      601 tcatctaaga aacgccgtat cgatttcact gaatcttcgt ctgataagtc atcttcgatt
      661 ttagcttctg gtagtagcag gggttttcac ggcgatagcg tcgttcagca aatcgacatg
      721 gcttttggta attcgaaccg tcaggagatt gatgaagatc tgcacagtcg ccagctcgcc
      781 gtctatggtc gtgagactat gaggcgtctc tttgcttcca atgttctcat ctcggggatg
      841 cacggtcttg gtgccgagat tgccaagaat cttatacttg ctggtgtgaa gtctgtgacc
      901 ttgcatgatg aaagagttgt agagctatgg gacttatcaa gcaactttgt tttctcggag
      961 gatgatgttg gcaagaatag agcagatgct tctgttcaga agttacagga tcttaacaat
     1021 gctgtggttg tctctagctt gaccaaaagc ttaaacaaag aggatctttc tggcttccag
     1081 gttgttgtat tctctgacat aagcatggaa agagcaatcg aatttgatga ctattgtcac
     1141 agccatcagc ctcctatcgc ttttgtcaag gctgatgtca ggggtctttt cggctctgtc
     1201 ttttgtgatt ttgggcctga atttgcggtt cttgatgttg atggagagga accccataca
     1261 ggcatcattg cgtctatctc taatgagaac caggcattta tttcctgtgt cgacgatgag
     1321 aggcttgagt ttgaagatgg tgacctagtt gttttctcag aagttgaggg tatgacagaa
     1381 ttgaatgatg ggaaaccaag gaagataaaa agtacacggc catattcatt cacccttgat
     1441 gaggacacta ctaactatgg aacatatgtg aagggtggta ttgtcactca agtgaaacag
     1501 ccaaagttac tgaatttcaa gccattgagg gaagcgctta aggatccagg ggattttcta
     1561 tttagtgatt tctctaagtt tgatcggcct ccactccttc acttagcatt ccaggcactt
     1621 gatcatttta aagctgaagc tggcagattt cctgttgctg gctcagaaga ggacgctcag
     1681 aagcttatat cgattgccac agccatcaat accgggcagg gtgatttaaa ggtggagaat
     1741 gttgatcaga agcttctaag acacttttcc tttggagcca aggctgtcct gaatcctatg
     1801 gctgcaatgt ttggcggtat tgttggacag gaggttgtta aagcttgctc tggaaaattt
     1861 catcctctct tccagttttt ctactttgat tcagtggagt cactcccctc tgaacctgtg
     1921 gactcgagtg actttgcacc aagaaacagc cggtatgatg cccaaatatc tgtatttggg
     1981 gccaagttcc agaagaaact tgaagatgct aaagttttca cagtagggtc tggtgctctt
     2041 ggctgtgagt tcttgaaaaa tttggctctg atgggggttt cttgtggaag ccaagggaag
     2101 ctgacagtga cagatgatga tatcattgag aagagtaacc tcagtcgtca atttctgttc
     2161 cgtgactgga acattggaca ggctaaatcc acagttgctg cttcggctgc tgctgttata
     2221 aatcctcgct tcaacattga ggccctgcaa aaccgtgttg gcgcggagac tgagaatgta
     2281 tttgacgatg ccttctggga gaacttaact gttgtcgtca atgcactgga taatgttaat
     2341 gcgaggctct atgttgattc gaggtgcttg tatttccaga agcctctcct tgagtctgga
     2401 actcttggta caaagtgcaa cacacagtcg gttatcccac atctaactga aaattacggt
     2461 gcctcaaggg acccaccaga gaaacaggcc cccatgtgta cagtgcattc cttcccgcat
     2521 aacattgatc actgtttaac ttgggctcgc tctgagtttg agggtctgct ggagaaaact
     2581 cccgctgaag tgaatgcata tctttctagc ccagttgagt acactaactc aatgatgagt
     2641 gctggtgatg ctcaggcgag ggacacattg gagaggattg ttgagtgcct tgaaaaggag
     2701 aagtgtgaaa ccttccaaga ctgcttaacc tgggcacgac tgaggtttga ggattacttt
     2761 gtgaaccgtg tcaagcaatt gatatacaca tttcctgaag atgctgcgac tagtaccgga
     2821 gctccattct ggtctgctcc aaaaagattc cctcgtccac tccagtactc gtcgtccgac
     2881 ccaagtctcc ttaactttat cacagcgact gctattttac gagcagagac atttgggatc
     2941 ccgatacctg agtggactaa gaacccaaag gaagcagcag aagctgttga cagagtgata
     3001 gtcccagact ttgagccaag gcaagatgca aagattgtga ctgatgagaa agctaccact
     3061 ttaaccactg cttcggtgga cgacgctgca gtcatcgacg atctcattgc taagatcgac
     3121 cagtgtaggc ataacttgtc gccagacttc aggatgaaac ctattcagtt cgaaaaggat
     3181 gatgatacaa actatcacat ggatgtgata gcaggactag ccaacatgag ggcgagaaat
     3241 tacagcatcc ctgaagtgga caagctgaaa gcaaagttca tagcagggag aatcatccca
     3301 gccattgcga cttcaacagc catggctact gggctggtct gcctcgagct gtacaaggtc
     3361 cttgatggag gacataaagt ggaagcttac agaaacacat ttgccaacct ggcacttcca
     3421 ctattctcca tggctgaacc acttccacca aaggtggtga agcaccgtga catggcttgg
     3481 acagtttggg acagatgggt tctcaaggga aacccaactc ttcgtgaggt gttgcagtgg
     3541 ctggaggaca aagggcttag cgcatacagc atctcttgtg gaagctgtct actgttcaac
     3601 agtatgttca caaggcacaa ggagaggatg gacaagaagg ttgtggatct cgctcgggat
     3661 gttgcgaaag tcgagttacc gccttatcgc aaccatcttg atgtagtggt ggcttgtgag
     3721 gatgaagatg acaatgacgt cgatattcct ctcgtctcta tctacttcag gtgagcttac
     3781 tattaacttt aagaaggaga tataccatgt acccatacga tgttcctgac tatgcgggct
     3841 atccctatga cgtcccggac tatgcaggat tgtatccata tgacgttcca gattacgcca
     3901 ctagagctgc ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
     3961 actatgcagg attgtatcca tatgacgttc cagattacgc aatgtttttc ttcttcttaa
     4021 ttcatctgac cattggattg gttttcgatt gcagagtgtt caattggaat ttggatcgtg
     4081 ggattttgga aatggcatcg aaacggattt tgaaggaatt gaaggatctg cagaaggatc
     4141 ctcctacttc atgtagcgca ggacccgttg cggaggacat gtttcattgg caggccacta
     4201 taatgggtcc atcggatagc ccttattctg gaggagtttt tcttgtaacc atccatttcc
     4261 ctccagatta cccatttaag cctcctaagg tggcttttag gacgaaggtg ttccatccaa
     4321 acattaacag caatggaagc atctgcctcg acatcttgaa ggagcagtgg agtcctgctc
     4381 tcacaatttc caaggtgctg ctatcgatct gttctttgtt aacggatcca aacccagatg
     4441 atcctttggt ccctgagata gctcacatgt acaagacaga caagaacaag tacgagtcca
     4501 ctgctcggac ctggacccaa aagtatgcca tgggctgagc ttttacgctt ttaagccagc
     4561 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     4621 cttacagaca agctgtgacc gggatgagat ccggctgcta acaaagcccg aaaggaagct
     4681 gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg
     4741 gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc
     4801 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     4861 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     4921 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     4981 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5041 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5101 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5161 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5221 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     5281 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt
     5341 cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
     5401 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
     5461 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
     5521 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
     5581 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     5641 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     5701 gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     5761 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     5821 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     5881 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     5941 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     6001 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     6061 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     6121 gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     6181 tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6241 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6301 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6361 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     6421 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     6481 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     6541 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     6601 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     6661 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     6721 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     6781 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     6841 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6901 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     6961 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7021 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     7081 ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat
     7141 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca
     7201 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     7261 acaagctgtg accgactccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     7321 acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc
     7381 tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct
     7441 gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta
     7501 agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat
     7561 acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc
     7621 ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa
     7681 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat
     7741 aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aacccaagac
     7801 cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc
     7861 gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct
     7921 caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg
     7981 cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa
     8041 gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc
     8101 gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaacac
     8161 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
     8221 gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt
     8281 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8341 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc
     8401 ttttcaccag tgacacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt
     8461 gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta
     8521 acggcgggat ataacatgag ctgtcctcgg tatcgtcgta tcccactacc gagatatccg
     8581 caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt
     8641 tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa
     8701 aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag
     8761 tgagatattt atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg
     8821 ctaacagcgc gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac
     8881 cgtcctcatg ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata
     8941 acgccggaac attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat
     9001 agttaatgat cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg
     9061 cttcgacgcc gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc
     9121 gagatttaat cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa
     9181 cgccaatcag caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat
     9241 tcagctccgc catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct
     9301 ggttcaccac gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata
     9361 acgttactgg tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca
     9421 taccgcgaaa ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc
     9481 gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca
     9541 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     9601 atacccacgc cgaaa
//
