LOCUS       sequence-20548-e         680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Alberti S, Gitler AD, Lindquist S
  TITLE     A suite of Gateway cloning vectors for high-throughput genetic 
            analysis in Saccharomyces cerevisiae.
  JOURNAL   Yeast. 2007 Oct . 24(10):913-9.
  PUBMED    17583893
REFERENCE   2  (bases 1 to 680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2007
            Oct . 24(10):913-9."
COMMENT     Sequence Label: pAG415GPD-ccdB-EGFP
FEATURES             Location/Qualifiers
     source          1..680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(36..160)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(168..184)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(168..184)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(190..453)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(273..296)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
ORIGIN
        1 aaagtgtaaa gcctgggggt gcctaatgcg gccgccatag tgactggata tgttgtgttt
       61 tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg atatttatat
      121 cattttacgt ttctcgttca gcttttttgt acaaacttgt gatgggggat ccactagttc
      181 tagaatccgt cgaaactaag ttctggtgtt ttaaaactaa aaaaaagact aactataaaa
      241 gtagaattta agaagtttaa gaaatagatt tacagaatta caatcaatac ctaccgtctt
      301 tatatactta ttagtcaagt aggggaataa tttcagggaa ctggtttcaa cctttttttt
      361 cagctttttc caaatcagag agagcagaag gtaatagaag gtgtaagaaa atgagataga
      421 tacatgcgtg ggtcaattgc cttgtgtcat catttactcc aggcaggttg catcactcca
      481 ttgaggttgt gcccgttttt tgcctgtttg tgcccctgtt ctctgtagtt gcgctaagag
      541 aatggaccta tgaactgatg gttggtgaag aaaacaatat tttggtgctg ggattctttt
      601 tttttctgga tgccagctta aaaagcgggc tccattatat ttagtggatg ccaggaataa
      661 actgttcacc cagacaccta
//
