LOCUS       sequence-205491-        9377 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  A lentiviral vector for expression in mammalian cells encoding 
            MEF2C-S222A insert.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Brown FC, Still E, Koche RP, Yim CY, Takao S, Cifani P, Reed C, 
            Gunasekera S, Ficarro SB, Romanienko P, Mark W, McCarthy C, de 
            Stanchina E, Gonen M, Seshan V, Bhola P, O'Donnell C, Spitzer B, 
            Stutzke C, Lavallee VP, Hebert J, Krivtsov AV, Melnick A, Paietta 
            EM, Tallman MS, Letai A, Sauvageau G, Pouliot G, Levine R, Marto JA,
            Armstrong SA, Kentsis A
  TITLE     MEF2C Phosphorylation Is Required for Chemotherapy Resistance in 
            Acute Myeloid Leukemia.
  JOURNAL   Cancer Discov. 2018 Apr;8(4):478-497. doi: 
            10.1158/2159-8290.CD-17-1271. Epub 2018 Feb 5.
  PUBMED    29431698
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/2159-8290.CD-17-1271"; journalName: "Cancer Discov"; date: 
            "2018-04"; volume: "8"; issue: "4"; pages: "478-497"
COMMENT     Sequence Label: pRecLV105 MEF2C S222A
FEATURES             Location/Qualifiers
     source          1..9377
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    93..210
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        259..769
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     688..706
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             791..1390
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(791..810)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1287..1307
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             1518..1751
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1823..1944
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1860..1879)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1914..1933
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1984..2119
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2139..2158)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2140..2158)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2168..2185)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2168..2184)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2177..2199)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2326..2781
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2413..2432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2623..2644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2807..2911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2912..2980,2981..3772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3130..3149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3943..4531
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4432..4451
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4685..4702
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4819..4840
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4855..4860,4861..4878,4879..4885)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4893..4909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4898..4920
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4917..4933
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4917..4933
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4952..4972
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4954..4972
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        5000..5226
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5227..5407
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5454..5579
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6072..6305
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6490..6534
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6832..7080
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6832..6852
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7003..7022
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        7235..7614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7615..7818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7769..7789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7815..7839
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    7897..7921
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(9371..9377,1..18))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttcttgtac aaagtggttg atcgcgtgca tgcgacgtca tagctctctc ccaattctcg
       61 acctcgagac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
      121 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
      181 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
      241 ctttggccgc ggctcgaggg ggttggggtt gcgccttttc caaggcagcc ctgggtttgc
      301 gcagggacgc ggctgctctg ggcgtggttc cgggaaacgc agcggcgccg accctgggtc
      361 tcgcacattc ttcacgtccg ttcgcagcgt cacccggatc ttcgccgcta cccttgtggg
      421 ccccccggcg acgcttcctg ctccgcccct aagtcgggaa ggttccttgc ggttcgcggc
      481 gtgccggacg tgacaaacgg aagccgcacg tctcactagt accctcgcag acggacagcg
      541 ccagggagca atggcagcgc gccgaccgcg atgggctgtg gccaatagcg gctgctcagc
      601 agggcgcgcc gagagcagcg gccgggaagg ggcggtgcgg gaggcggggt gtggggcggt
      661 agtgtgggcc ctgttcctgc ccgcgcggtg ttccgcattc tgcaagcctc cggagcgcac
      721 gtcggcagtc ggctccctcg ttgaccgaat caccgacctc tctccccagg gggatccacc
      781 ggagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
      841 cagggcggta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
      901 cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
      961 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     1021 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     1081 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     1141 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     1201 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     1261 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     1321 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     1381 cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca
     1441 tgcatcggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     1501 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt
     1561 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     1621 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     1681 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     1741 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     1801 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     1861 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     1921 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     1981 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     2041 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     2101 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     2161 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     2221 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     2281 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     2341 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2401 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2461 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2521 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     2581 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2641 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     2701 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2761 aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat
     2821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     2881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     2941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     3001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     3061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     3121 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     3181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     3241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     3301 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     3361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     3421 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     3481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     3541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     3601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     3661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     3721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     3781 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     3841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     3901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     3961 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4021 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4081 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4141 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4201 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4261 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     4321 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     4381 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     4441 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     4501 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     4561 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     4621 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     4681 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
     4741 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     4801 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     4861 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     4921 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
     4981 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
     5041 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
     5101 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
     5161 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
     5221 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5281 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5341 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5401 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     5461 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     5521 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     5581 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     5641 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     5701 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     5761 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     5821 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     5881 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     5941 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     6001 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     6061 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     6121 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     6181 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     6241 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     6301 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     6361 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     6421 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     6481 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     6541 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     6601 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     6661 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     6721 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     6781 tctcgacggt atcgatcacg agactagcct cgagcggccg cccccttcac cgagggccta
     6841 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
     6901 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
     6961 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
     7021 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
     7081 ggtatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
     7141 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
     7201 tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa
     7261 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     7321 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     7381 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     7441 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
     7501 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     7561 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg
     7621 cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt
     7681 ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca
     7741 aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag
     7801 gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc
     7861 tgttttgacc tccatagaag attctagatt cgaagaacaa gtttgtacaa aaaagcagaa
     7921 gggccgtcaa ggcccaccat ggggagaaaa aagattcaga ttacgaggat tatggatgaa
     7981 cgtaacagac aggtgacatt tacaaagagg aaatttgggt tgatgaagaa ggcttatgag
     8041 ctgagcgtgc tgtgtgactg tgagattgcg ctgatcatct tcaacagcac caacaagctg
     8101 ttccagtatg ccagcaccga catggacaaa gtgcttctca agtacacgga gtacaacgag
     8161 ccgcatgaga gccggacaaa ctcagacatc gtggagacgt tgagaaagaa gggccttaat
     8221 ggctgtgaca gcccagaccc cgatgcggac gattccgtag gtcacagccc tgagtctgag
     8281 gacaagtaca ggaaaattaa cgaagatatt gatctaatga tcagcaggca aagattgtgt
     8341 gctgttccac ctcccaactt cgagatgcca gtctccatcc cagtgtccag ccacaacagt
     8401 ttggtgtaca gcaaccctgt cagctcactg ggaaacccca acctattgcc actggctcac
     8461 ccttctctgc agaggaatag tatgtctcct ggtgtaacac atcgacctcc aagtgcaggt
     8521 aacacaggtg gtctgatggg tggagacctc acgtctggtg caggcaccag tgcagggaac
     8581 gggtatggca atccccgaaa cgcaccaggt ctgctggtct cacctggtaa cttgaacaag
     8641 aatatgcaag caaaatctcc tcccccaatg aatttaggaa tgaataaccg taaaccagat
     8701 ctccgagttc ttattccacc aggcagcaag aatacgatgc catcagtgtc tgaggatgtc
     8761 gacctgcttt tgaatcaaag gataaataac tcccagtcgg ctcagtcatt ggctacccca
     8821 gtggtttccg tagcaactcc tactttacca ggacaaggaa tgggaggata tccatcagcc
     8881 atttcaacaa catatggtac cgagtactct ctgagtagtg cagacctgtc atctctgtct
     8941 gggtttaaca ccgccagcgc tcttcacctt ggttcagtaa ctggctggca acagcaacac
     9001 ctacataaca tgccaccatc tgccctcagt cagttgggag cttgcactag cactcattta
     9061 tctcagagtt caaatctctc cctgccttct actcaaagcc tcaacatcaa gtcagaacct
     9121 gtttctcctc ctagagaccg taccaccacc ccttcgagat acccacaaca cacgcgccac
     9181 gaggcgggga gatctcctgt tgacagcttg agcagctgta gcagttcgta cgacgggagc
     9241 gaccgagagg atcaccggaa cgaattccac tcccccattg gactcaccag accttcgccg
     9301 gacgaaaggg aaagtccctc agtcaagcgc atgcgacttt ctgaaggatg ggcaacataa
     9361 ggcctcatgg gcccagc
//
