LOCUS       sequence-205493-       16592 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that 
            targets the FWA promoter in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16592)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 16592)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF108_3xFLAG_TET1cd
FEATURES             Location/Qualifiers
     source          1..16592
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      408..602
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(668..1741)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2170..2799)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4100..4124
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4312..4334
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4326..4343
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4327..4343
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6441..6485
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6513..6536
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7047..7118
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    7158..7182
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9385..9409)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10221..10237)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10221..10237)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10234..10256)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10245..10261
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10269..10275,10276..10293,10294..10299))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10314..10335
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10526..11202
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11139..11159
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11247..11798
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11805..11979
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12057..12081
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12506..13300
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14725..15313
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15214..15233
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15467..15484
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15499..15639
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15565..15584)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15725..15747
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(15801..16592)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
       61 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
      121 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      181 gcgttcggtc aaggttctgg accagttgcg tgagcgcatg gcgccggcgg tcgagtggcg
      241 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
      301 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
      361 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
      421 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
      481 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
      541 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
      601 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
      661 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
      721 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
      781 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
      841 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
      901 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
      961 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     1021 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     1081 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     1141 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     1201 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     1261 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     1321 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     1381 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     1441 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     1501 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     1561 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     1621 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     1681 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
     1741 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
     1801 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     1861 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
     1921 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
     1981 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
     2041 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
     2101 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
     2161 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
     2221 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
     2281 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
     2341 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
     2401 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
     2461 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
     2521 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
     2581 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
     2641 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
     2701 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
     2761 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
     2821 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
     2881 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
     2941 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
     3001 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
     3061 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
     3121 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
     3181 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
     3241 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
     3301 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
     3361 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
     3421 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
     3481 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
     3541 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
     3601 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
     3661 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
     3721 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
     3781 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
     3841 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
     3901 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
     3961 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
     4021 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
     4081 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
     4141 actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca
     4201 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4261 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     4321 gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac tctagaggat
     4381 ccccgggtac cgggcccccc ctcgaggcgc gccacgcgta gtctagctca acagagcttt
     4441 taacccaaat tggtacaata gaatacaact ttagatcata attctcaaaa gaaagagatt
     4501 ccttagctat tctatctgcc actccatttc cttctcggct tgtatgcaca agcataaaat
     4561 cctcaaactt gctaagtaga tactttatgt cttggataat tggattgaga cttgacaagc
     4621 ataactttca tgtaaccaaa gacacaagtt gctgagaatc cacctcaaaa atgatcttcc
     4681 tataattgaa tcgggataat gacagcacag cccatctaag agcctccact tctacttcca
     4741 gcacgcttct tacttttacc acagctcttg cacctaacca taacaccttc cctgtatgat
     4801 cgcgaagcac ccaccctaag ccacatttta atccttctgt tggccatgcc ccatcaaagt
     4861 tgcacttaac ccaagattgt ggtggagctt cccatgtttc tcgtctgtcc cgacggtgtt
     4921 gtggttggtg ctttccttac attctgagcc tctttccttc taatccactc atctgcatct
     4981 tcttgtgtcc ttactaatac ctcattggtt ccaaattccc tccctttaag caccagctcg
     5041 tttctgttct tccacagcct cccaagtatc caagggacta aagcctccac attcttcaga
     5101 tcaggatatt cttgtttaag atgttgaact ctatggaggt ttgtatgaac tgatgatcta
     5161 ggaccggata agttcccttc ttcatagcga acttattcaa agaatgtttt gtgtatcatt
     5221 cttgttacat tgttattaat gaaaaaatat tattggtcat tggactgaac acgagtgtta
     5281 aatatggacc aggccccaaa taagatccat tgatatatga attaaataac aagaataaat
     5341 cgagtcacca aaccacttgc cttttttaac gagacttgtt caccaacttg atacaaaagt
     5401 cattatccta tgcaaatcaa taatcataca aaaatatcca ataacactaa aaaattaaaa
     5461 gaaatggata atttcacaat atgttatacg ataaagaagt tacttttcca agaaattcac
     5521 tgattttata agcccacttg cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa
     5581 taaagcacga agaattctag aaaatacgaa atacgcttca atgcagtggg acccacggtt
     5641 caattattgc caattttcag ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa
     5701 aaatataaat cgtaacgatc gttaaatctc aacggctgga tcttatgacg accgttagaa
     5761 attgtggttg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac
     5821 gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt
     5881 agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt attattagct
     5941 attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct
     6001 tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa
     6061 atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt
     6121 attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg
     6181 tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat
     6241 cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt
     6301 gtcgaataat tactcttcga tttgtgattt ctatctagat ctggtgttag tttctagttt
     6361 gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagacgcg tccatggttg
     6421 ggtaccaaca aatggctggt ggacttaacg atatcttcga agctcagaag attgaatggc
     6481 atgaggatac tggtggatct agcatccccg gacttgaggt tcttttccaa ggacctctcg
     6541 agccttataa atgccctgag tgtggaaaat ctttctctca atctggagat cttaggaggc
     6601 accaaaggac ccataccggt gaaaaacctt ataaatgccc tgagtgtgga aaatcattca
     6661 gtaggagtga tcatcttacc acccatcaga ggacccatac tggtgaaaaa ccttataaat
     6721 gccctgagtg tggaaaatca ttctcacaat cttcatctct tgttaggcat cagaggactc
     6781 ataccggaga aaaaccttat aaatgccctg agtgtggaaa atcattctca acatctggaa
     6841 accttgttag gcaccaaagg acccatactg gtgaaaaacc ttataaatgc cctgagtgtg
     6901 gaaaatcatt ctctcagaga gcaaacctta gggctcatca aaggacacac actggagaaa
     6961 aaccttataa atgccctgag tgtggaaaat ctttctctag gagtgataag ctcaccgagc
     7021 atcaaaggac tcacaccgga ctcgaggact acaaagacga tgacgacaaa gactacaaag
     7081 acgatgacga caaagactac aaagacgatg acgacaaaaa gcttgatatc gccatggggg
     7141 gcgcgccaag ctatcaaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca
     7201 ccatgggatc cctgcccacc tgcagctgtc ttgatcgagt tatacaaaaa gacaaaggcc
     7261 catattatac acaccttggg gcaggaccaa gtgttgctgc tgtcagggaa atcatggaga
     7321 ataggtatgg tcaaaaagga aacgcaataa ggatagaaat agtagtgtac accggtaaag
     7381 aagggaaaag ctctcatggg tgtccaattg ctaagtgggt tttaagaaga agcagtgatg
     7441 aagaaaaagt tctttgtttg gtccggcagc gtacaggcca ccactgtcca actgctgtga
     7501 tggtggtgct catcatggtg tgggatggca tccctcttcc aatggccgac cggctataca
     7561 cagagctcac agagaatcta aagtcataca atgggcaccc taccgacaga agatgcaccc
     7621 tcaatgaaaa tcgtacctgt acatgtcaag gaattgatcc agagacttgt ggagcttcat
     7681 tctcttttgg ctgttcatgg agtatgtact ttaatggctg taagtttggt agaagcccaa
     7741 gccccagaag atttagaatt gatccaagct ctcccttaca tgaaaaaaac cttgaagata
     7801 acttacagag tttggctaca cgattagctc caatttataa gcagtatgct ccagtagctt
     7861 accaaaatca ggtggaatat gaaaatgttg cccgagaatg tcggcttggc agcaaggaag
     7921 gtcgaccctt ctctggggtc actgcttgcc tggacttctg tgctcatccc cacagggaca
     7981 ttcacaacat gaataatgga agcactgtgg tttgtacctt aactcgagaa gataaccgct
     8041 ctttgggtgt tattcctcaa gatgagcagc tccatgtgct acctctttat aagctttcag
     8101 acacagatga gtttggctcc aaggaaggaa tggaagccaa gatcaaatct ggggccatcg
     8161 aggtcctggc accccgccgc aaaaaaagaa cgtgtttcac tcagcctgtt ccccgttctg
     8221 gaaagaagag ggctgcgatg atgacagagg ttcttgcaca taagataagg gcagtggaaa
     8281 agaaacctat tccccgaatc aagcggaaga ataactcaac aacaacaaac aacagtaagc
     8341 cttcgtcact gccaacctta gggagtaaca ctgagaccgt gcaacctgaa gtaaaaagtg
     8401 aaaccgaacc ccattttatc ttaaaaagtt cagacaacac taaaacttat tcgctgatgc
     8461 catccgctcc tcacccagtg aaagaggcat ctccaggctt ctcctggtcc ccgaagactg
     8521 cttcagccac accagctcca ctgaagaatg acgcaacagc ctcatgcggg ttttcagaaa
     8581 gaagcagcac tccccactgt acgatgcctt cgggaagact cagtggtgcc aatgctgcag
     8641 ctgctgatgg ccctggcatt tcacagcttg gcgaagtggc tcctctcccc accctgtctg
     8701 ctcctgtgat ggagcccctc attaattctg agccttccac tggtgtgact gagccgctaa
     8761 cgcctcatca gccaaaccac cagccctcct tcctcacctc tcctcaagac cttgcctctt
     8821 ctccaatgga agaagatgag cagcattctg aagcagatga gcctccatca gacgaacccc
     8881 tatctgatga ccccctgtca cctgctgagg agaaattgcc ccacattgat gagtattggt
     8941 cagacagtga gcacatcttt ttggatgcaa atattggtgg ggtggccatc gcacctgctc
     9001 acggctcggt tttgattgag tgtgcccggc gagagctgca cgctaccact cctgttgagc
     9061 accccaaccg taatcatcca acccgcctct cccttgtctt ttaccagcac aaaaacctaa
     9121 ataagcccca acatggtttt gaactaaaca agattaagtt tgaggctaaa gaagctaaga
     9181 ataagaaaat gaaggcctca gagcaaaaag accaggcagc taatgaaggt ccagaacagt
     9241 cctctgaagt aaatgaattg aaccaaattc cttctcataa agcattaaca ttaacccatg
     9301 acaatgttgt caccgtgtcc ccttatgctc tcacacacgt tgcggggccc tataaccatt
     9361 gggtctgaaa gggtgggcgc gccgacccag ctttcttgta caaagtggtt cgataattct
     9421 taattaacta gttctagagc ggccgccacc gcggtggagc tcctaggtga gtctagagag
     9481 ttaattaaga cccgggacta gtccctagag tcctgcttta atgagatatg cgagacgcct
     9541 atgatcgcat gatatttgct ttcaattctg ttgtgcacgt tgtaaaaaac ctgagcatgt
     9601 gtagctcaga tccttaccgc cggtttcggt tcattctaat gaatatatca cccgttacta
     9661 tcgtattttt atgaataata ttctccgttc aatttactga ttgtacccta ctacttatat
     9721 gtacaatatt aaaatgaaaa caatatattg tgctgaatag gtttatagcg acatctatga
     9781 tagagcgcca caataacaaa caattgcgtt ttattattac aaatccaatt ttaaaaaaag
     9841 cggcagaacc ggtcaaacct aaaagactga ttacataaat cttattcaaa tttcaaaagt
     9901 gccccagggg ctagtatcta cgacacaccg agcggcgaac taataacgct cactgaaggg
     9961 aactccggtt ccccgccggc gcgcatgggt gagattcctt gaagttgagt attggccgtc
    10021 cgctctaccg aaagttacgg gcaccattca acccggtcca gcacggcggc cgggtaaccg
    10081 acttgctgcc ccgagaatta tgcagcattt ttttggtgta tgtgggcccc aaatgaagtg
    10141 caggtcaaac cttgacagtg acgacaaatc gttgggcggg tccagggcga attttgcgac
    10201 gagctcgaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
    10261 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
    10321 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
    10381 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctag
    10441 agcagcttgc caacatggtg gagcacgaca ctctcgtcta ctccaagaat atcaaagata
    10501 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaata tcgggaaacc
    10561 tcctcggatt ccattgccca gctatctgtc acttcatcaa aaggacagta gaaaaggaag
    10621 gtggcaccta caaatgccat cattgcgata aaggaaaggc tatcgttcaa gatgcctctg
    10681 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
    10741 ttccaaccac gtcttcaaag caagtggatt gatgtgaaca tggtggagca cgacactctc
    10801 gtctactcca agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt
    10861 caacaaaggg taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc
    10921 atcaaaagga cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga
    10981 aaggctatcg ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg
    11041 aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt
    11101 gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca agacccttcc
    11161 tctatataag gaagttcatt tcatttggag aggacacgct gaaatcacca gtctctctct
    11221 acaaatctat ctctctcgag tctaccatga gcccagaacg acgcccggcc gacatccgcc
    11281 gtgccaccga ggcggacatg ccggcggtct gcaccatcgt caaccactac atcgagacaa
    11341 gcacggtcaa cttccgtacc gagccgcagg aaccgcagga gtggacggac gacctcgtcc
    11401 gtctgcggga gcgctatccc tggctcgtcg ccgaggtgga cggcgaggtc gccggcatcg
    11461 cctacgcggg cccctggaag gcacgcaacg cctacgactg gacggccgag tcgaccgtgt
    11521 acgtctcccc ccgccaccag cggacgggac tgggctccac gctctacacc cacctgctga
    11581 agtccctgga ggcacagggc ttcaagagcg tggtcgctgt catcgggctg cccaacgacc
    11641 cgagcgtgcg catgcacgag gcgctcggat atgccccccg cggcatgctg cgggcggccg
    11701 gcttcaagca cgggaactgg catgacgtgg gtttctggca gctggacttc agcctgccgg
    11761 taccgccccg tccggtcctg cccgtcaccg agatttgact cgagtttctc cataataatg
    11821 tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc atgtgttgag
    11881 catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca ataaaatttc
    11941 taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa ttcggcgtta
    12001 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
    12061 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
    12121 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
    12181 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
    12241 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
    12301 gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg
    12361 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
    12421 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
    12481 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
    12541 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
    12601 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
    12661 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
    12721 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
    12781 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
    12841 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
    12901 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
    12961 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
    13021 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
    13081 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
    13141 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
    13201 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
    13261 gaaaataaaa tattatattt tactggatga attgttttag tacctagaat gcatgaccaa
    13321 aatcccttaa cgctgatgaa tcccctaatg atttttatca aaatcattaa gttaaggtag
    13381 atacacatct tgtcatatga tcaaatggtt tcgccaaaaa tcaataatca gacaacaaaa
    13441 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
    13501 aacgactcaa cagcttaacg ttggcttgcc acgccttact tgactgtaaa actctcactc
    13561 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
    13621 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
    13681 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
    13741 tccgtgagca aaaacggctt atggtattgc gagcttcagt cgcactacac ggtcgttctg
    13801 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
    13861 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
    13921 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
    13981 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
    14041 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
    14101 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
    14161 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
    14221 caaaggagcc atggattcta gcaactaact tacctgttga aattcgaaca cccaaacaac
    14281 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
    14341 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
    14401 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
    14461 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
    14521 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
    14581 tactcgtggc tgcaacccta ctagctcaaa atttattcac acatggttac gctttgggga
    14641 aattatgagg ggatctctca gcccttaacg tgagttttcg ttccactgag cgtcagaccc
    14701 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
    14761 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
    14821 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
    14881 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
    14941 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
    15001 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
    15061 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
    15121 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
    15181 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    15241 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    15301 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    15361 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    15421 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    15481 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    15541 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    15601 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
    15661 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    15721 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
    15781 ggtgccttga tgtgggcgcc ttatttgccg actaccttgg tgatctcgcc tttcacgtag
    15841 tggacaaatt cttccaactg atctgcgcgc gaggccaagc gatcttcttc ttgtccaaga
    15901 taagcctgtc tagcttcaag tatgacgggc tgatactggg ccggcaggcg ctccattgcc
    15961 cagtcggcag cgacatcctt cggcgcgatt ttgccggtta ctgcgctgta ccaaatgcgg
    16021 gacaacgtaa gcactacatt tcgctcatcg ccagcccagt cgggcggcga gttccatagc
    16081 gttaaggttt catttagcgc ctcaaataga tcctgttcag gaaccggatc aaagagttcc
    16141 tccgccgctg gacctaccaa ggcaacgcta tgttctcttg cttttgtcag caagatagcc
    16201 agatcaatgt cgatcgtggc tggctcgaag atacctgcaa gaatgtcatt gcgctgccat
    16261 tctccaaatt gcagttcgcg cttagctgga taacgccacg gaatgatgtc gtcgtgcaca
    16321 acaatggtga cttctacagc gcggagaatc tcgctctctc caggggaagc cgaagtttcc
    16381 aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa gccttacggt caccgtaacc
    16441 agcaaatcaa tatcactgtg tggcttcagg ccgccatcca ctgcggagcc gtacaaatgt
    16501 acggccagca acgtcggttc gagatggcgc tcgatgacgc caactacctc tgatagttga
    16561 gtcgatactt cggcgatcac cgcttccctc at
//
