LOCUS       sequence-205495-        9579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9579)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-304
FEATURES             Location/Qualifiers
     source          1..9579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     971..990
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1010..1087)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1293..1312
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1396..1415
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1396..1414
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1415..1439
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1493..1510
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5058..5147
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5160..5249
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5909..5927)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5923..5970
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6007..6462
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6094..6113)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6304..6325
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6554..7369)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6554..7369)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7277..7296
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7361..7380
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7491..8079
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7980..7999
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8233..8250
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8265..8404
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8330..8349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8490..8512
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8506..8697)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9506..9579,1..1009))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgacacg ggcaacagct
       61 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
      121 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtcct
      181 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
      241 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
      301 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
      361 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
      421 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
      481 atgcgaccag atgctccacg cccagtcgcg taccgtcctc atgggagaaa ataatactgt
      541 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
      601 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
      661 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
      721 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
      781 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
      841 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
      901 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
      961 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
     1021 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
     1081 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
     1141 agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg
     1201 cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg
     1261 agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca
     1321 accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc
     1381 tcgatcccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc
     1441 ctctagaaat aattttgcct taactttaag aaggagatat accatgggca gccaccacca
     1501 ccaccaccac agcggaatgc agatctttgt taagactctc accggaaaga caatcaccct
     1561 agaggtggaa agctccgaca ccatcgacaa cgttaaggcc aagatccagg ataaggaggg
     1621 cattcctccg gatcagcaga ggcttatttt cgccggcaag cagctagagg atggccgtac
     1681 gttggctgat tacaatatcc agaaggaatc caccctccac ttggtcctca ggctccgtgg
     1741 tggttaagct tgcaattaac tttaagaagg agatatacaa tgcttcacaa gcgagctagt
     1801 gaagctaacg ataagaacga taataccatc atcggttccg atttagcatc atctaagaaa
     1861 cgccgtatcg atttcactga atcttcgtct gataagtcat cttcgatttt agcttctggt
     1921 agtagcaggg gttttcacgg cgatagcgtc gttcagcaaa tcgacatggc ttttggtaat
     1981 tcgaaccgtc aggagattga tgaagatctg cacagtcgcc agctcgccgt ctatggtcgt
     2041 gagactatga ggcgtctctt tgcttccaat gttctcatct cggggatgca cggtcttggt
     2101 gccgagattg ccaagaatct tatacttgct ggtgtgaagt ctgtgacctt gcatgatgaa
     2161 agagttgtag agctatggga cttatcaagc aactttgttt tctcggagga tgatgttggc
     2221 aagaatagag cagatgcttc tgttcagaag ttacaggatc ttaacaatgc tgtggttgtc
     2281 tctagcttga ccaaaagctt aaacaaagag gatctttctg gcttccaggt tgttgtattc
     2341 tctgacataa gcatggaaag agcaatcgaa tttgatgact attgtcacag ccatcagcct
     2401 cctatcgctt ttgtcaaggc tgatgtcagg ggtcttttcg gctctgtctt ttgtgatttt
     2461 gggcctgaat ttgcggttct tgatgttgat ggagaggaac cccatacagg catcattgcg
     2521 tctatctcta atgagaacca ggcatttatt tcctgtgtcg acgatgagag gcttgagttt
     2581 gaagatggtg acctagttgt tttctcagaa gttgagggta tgacagaatt gaatgatggg
     2641 aaaccaagga agataaaaag tacacggcca tattcattca cccttgatga ggacactact
     2701 aactatggaa catatgtgaa gggtggtatt gtcactcaag tgaaacagcc aaagttactg
     2761 aatttcaagc cattgaggga agcgcttaag gatccagggg attttctatt tagtgatttc
     2821 tctaagtttg atcggcctcc actccttcac ttagcattcc aggcacttga tcattttaaa
     2881 gctgaagctg gcagatttcc tgttgctggc tcagaagagg acgctcagaa gcttatatcg
     2941 attgccacag ccatcaatac cgggcagggt gatttaaagg tggagaatgt tgatcagaag
     3001 cttctaagac acttttcctt tggagccaag gctgtcctga atcctatggc tgcaatgttt
     3061 ggcggtattg ttggacagga ggttgttaaa gcttgctctg gaaaatttca tcctctcttc
     3121 cagtttttct actttgattc agtggagtca ctcccctctg aacctgtgga ctcgagtgac
     3181 tttgcaccaa gaaacagccg gtatgatgcc caaatatctg tatttggggc caagttccag
     3241 aagaaacttg aagatgctaa agttttcaca gtagggtctg gtgctcttgg ctgtgagttc
     3301 ttgaaaaatt tggctctgat gggggtttct tgtggaagcc aagggaagct gacagtgaca
     3361 gatgatgata tcattgagaa gagtaacctc agtcgtcaat ttctgttccg tgactggaac
     3421 attggacagg ctaaatccac agttgctgct tcggctgctg ctgttataaa tcctcgcttc
     3481 aacattgagg ccctgcaaaa ccgtgttggc gcggagactg agaatgtatt tgacgatgcc
     3541 ttctgggaga acttaactgt tgtcgtcaat gcactggata atgttaatgc gaggctctat
     3601 gttgattcga ggtgcttgta tttccagaag cctctccttg agtctggaac tcttggtaca
     3661 aagtgcaaca cacagtcggt tatcccacat ctaactgaaa attacggtgc ctcaagggac
     3721 ccaccagaga aacaggcccc catgtgtaca gtgcattcct tcccgcataa cattgatcac
     3781 tgtttaactt gggctcgctc tgagtttgag ggtctgctgg agaaaactcc cgctgaagtg
     3841 aatgcatatc tttctagccc agttgagtac actaactcaa tgatgagtgc tggtgatgct
     3901 caggcgaggg acacattgga gaggattgtt gagtgccttg aaaaggagaa gtgtgaaacc
     3961 ttccaagact gcttaacctg ggcacgactg aggtttgagg attactttgt gaaccgtgtc
     4021 aagcaattga tatacacatt tcctgaagat gctgcgacta gtaccggagc tccattctgg
     4081 tctgctccaa aaagattccc tcgtccactc cagtactcgt cgtccgaccc aagtctcctt
     4141 aactttatca cagcgactgc tattttacga gcagagacat ttgggatccc gatacctgag
     4201 tggactaaga acccaaagga agcagcagaa gctgttgaca gagtgatagt cccagacttt
     4261 gagccaaggc aagatgcaaa gattgtgact gatgagaaag ctaccacttt aaccactgct
     4321 tcggtggacg acgctgcagt catcgacgat ctcattgcta agatcgacca gtgtaggcat
     4381 aacttgtcgc cagacttcag gatgaaacct attcagttcg aaaaggatga tgatacaaac
     4441 tatcacatgg atgtgatagc aggactagcc aacatgaggg cgagaaatta cagcatccct
     4501 gaagtggaca agctgaaagc aaagttcata gcagggagaa tcatcccagc cattgcgact
     4561 tcaacagcca tggctactgg gctggtctgc ctcgagctgt acaaggtcct tgatggagga
     4621 cataaagtgg aagcttacag aaacacattt gccaacctgg cacttccact attctccatg
     4681 gctgaaccac ttccaccaaa ggtggtgaag caccgtgaca tggcttggac agtttgggac
     4741 agatgggttc tcaagggaaa cccaactctt cgtgaggtgt tgcagtggct ggaggacaaa
     4801 gggcttagcg catacagcat ctcttgtgga agctgtctac tgttcaacag tatgttcaca
     4861 aggcacaagg agaggatgga caagaaggtt gtggatctcg ctcgggatgt tgcgaaagtc
     4921 gagttaccgc cttatcgcaa ccatcttgat gtagtggtgg cttgtgagga tgaagatgac
     4981 aatgacgtcg atattcctct cgtctctatc tacttcaggt gagcttacta ttaactttaa
     5041 gaaggagata taccatgtac ccatacgatg ttcctgacta tgcgggctat ccctatgacg
     5101 tcccggacta tgcaggattg tatccatatg acgttccaga ttacgccact agagctgctt
     5161 acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat
     5221 tgtatccata tgacgttcca gattacgcaa tgaactcaca agcttgtcta cttcttcaga
     5281 aacagcttaa agatctttgt aagcatcctg tagatggatt ctctgctggt ttagttgatg
     5341 agaagaatat attcgaatgg agcgtaacta ttattggacc tcctgatact ctctatgaag
     5401 gaggattttt ctatgccatt atgtcattcc cacaaaacta tcctaatagc ccacctacgg
     5461 tgcggtttac ttcagatatt tggcatccta acgtttatcc cgatggccgc gtttgtatat
     5521 ctattctaca tcctcctggt gatgatccaa gtggatatga gcttgcaagt gagcgttgga
     5581 cgcctgttca cacagttgag agtatcatgt tgagtattat atcaatgctc tctggtccca
     5641 acgacgaatc tccagcaaac gttgaggctg ctaaagaatg gagagagaag agagatgagt
     5701 tcaagaagaa agtgagccgt tgtgtcagaa agtctcaaga aatgttttga gcttttacgc
     5761 ttttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     5821 tcccggcatc cgcttacaga caagctgtga ccgggatgag atccggctgc taacaaagcc
     5881 cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg
     5941 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggattggcg
     6001 aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     6061 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc
     6121 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     6181 gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta
     6241 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     6301 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg
     6361 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa
     6421 aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg
     6481 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     6541 catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     6601 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     6661 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     6721 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     6781 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     6841 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     6901 tcattcgtga ttgcgcctga gcgagtcgaa atacgcgatc gctgttaaaa ggacaattac
     6961 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     7021 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     7081 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     7141 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     7201 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     7261 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     7321 aatttaatcg cggcctagag caagacgttt cccgttgaat atggctcata acaccccttg
     7381 tattactgtt tatgtaagca gacagtttta ttgttcatga ccaaaatccc ttaacgtgag
     7441 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     7501 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     7561 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     7621 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     7681 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     7741 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     7801 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     7861 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     7921 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     7981 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     8041 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     8101 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     8161 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     8221 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     8281 ctccttacgc atctgtgcgg tatttcacac cgcaatggtg cactctcagt acaatctgct
     8341 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc
     8401 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     8461 atccgcttac agacaagctg tgaccgactc cgggagctgc atgtgtcaga ggttttcacc
     8521 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga
     8581 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa
     8641 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     8701 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag
     8761 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg
     8821 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca
     8881 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca
     8941 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg
     9001 gaaacccaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct
     9061 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc
     9121 tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggggccg ccatgccggc
     9181 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc
     9241 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg
     9301 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat
     9361 gataaagaac acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga
     9421 gctgactggg ttgaaggctc tcaagggcat cggtcgagat cccggtgcct aatgagtgag
     9481 ctaacttaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     9541 ccagctgcat taatgaatcg gccaacgcgc ggggagagg
//
