LOCUS       sequence-205497-        9582 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9582)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9582)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-305
FEATURES             Location/Qualifiers
     source          1..9582
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     967..986
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1006..1083)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1289..1308
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1392..1411
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1392..1410
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1411..1435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1489..1506
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5054..5143
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5156..5245
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5908..5926)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5922..5969
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6006..6461
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6093..6112)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6303..6324
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6553..7368)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6553..7368)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7276..7295
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7360..7379
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7490..8078
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7979..7998
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8232..8249
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8264..8403
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8329..8348)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8489..8511
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8505..8696)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9505..9582,1..1005))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gacacgggca acagctgatt
       61 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
      121 caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcctcggt
      181 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
      241 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
      301 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
      361 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
      421 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
      481 gaccagatgc tccacgccca gtcgcgtacc gtcctcatgg gagaaaataa tactgttgat
      541 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
      601 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
      661 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
      721 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
      781 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
      841 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
      901 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
      961 gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa
     1021 ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc attcgatggt
     1081 gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta
     1141 ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca
     1201 acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc
     1261 cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg
     1321 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
     1381 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     1441 agaaataatt ttgccttaac tttaagaagg agatatacca tgggcagcca ccaccaccac
     1501 caccacagcg gaatgcagat ctttgttaag actctcaccg gaaagacaat caccctagag
     1561 gtggaaagct ccgacaccat cgacaacgtt aaggccaaga tccaggataa ggagggcatt
     1621 cctccggatc agcagaggct tattttcgcc ggcaagcagc tagaggatgg ccgtacgttg
     1681 gctgattaca atatccagaa ggaatccacc ctccacttgg tcctcaggct ccgtggtggt
     1741 taagcttgca attaacttta agaaggagat atacaatgct tcacaagcga gctagtgaag
     1801 ctaacgataa gaacgataat accatcatcg gttccgattt agcatcatct aagaaacgcc
     1861 gtatcgattt cactgaatct tcgtctgata agtcatcttc gattttagct tctggtagta
     1921 gcaggggttt tcacggcgat agcgtcgttc agcaaatcga catggctttt ggtaattcga
     1981 accgtcagga gattgatgaa gatctgcaca gtcgccagct cgccgtctat ggtcgtgaga
     2041 ctatgaggcg tctctttgct tccaatgttc tcatctcggg gatgcacggt cttggtgccg
     2101 agattgccaa gaatcttata cttgctggtg tgaagtctgt gaccttgcat gatgaaagag
     2161 ttgtagagct atgggactta tcaagcaact ttgttttctc ggaggatgat gttggcaaga
     2221 atagagcaga tgcttctgtt cagaagttac aggatcttaa caatgctgtg gttgtctcta
     2281 gcttgaccaa aagcttaaac aaagaggatc tttctggctt ccaggttgtt gtattctctg
     2341 acataagcat ggaaagagca atcgaatttg atgactattg tcacagccat cagcctccta
     2401 tcgcttttgt caaggctgat gtcaggggtc ttttcggctc tgtcttttgt gattttgggc
     2461 ctgaatttgc ggttcttgat gttgatggag aggaacccca tacaggcatc attgcgtcta
     2521 tctctaatga gaaccaggca tttatttcct gtgtcgacga tgagaggctt gagtttgaag
     2581 atggtgacct agttgttttc tcagaagttg agggtatgac agaattgaat gatgggaaac
     2641 caaggaagat aaaaagtaca cggccatatt cattcaccct tgatgaggac actactaact
     2701 atggaacata tgtgaagggt ggtattgtca ctcaagtgaa acagccaaag ttactgaatt
     2761 tcaagccatt gagggaagcg cttaaggatc caggggattt tctatttagt gatttctcta
     2821 agtttgatcg gcctccactc cttcacttag cattccaggc acttgatcat tttaaagctg
     2881 aagctggcag atttcctgtt gctggctcag aagaggacgc tcagaagctt atatcgattg
     2941 ccacagccat caataccggg cagggtgatt taaaggtgga gaatgttgat cagaagcttc
     3001 taagacactt ttcctttgga gccaaggctg tcctgaatcc tatggctgca atgtttggcg
     3061 gtattgttgg acaggaggtt gttaaagctt gctctggaaa atttcatcct ctcttccagt
     3121 ttttctactt tgattcagtg gagtcactcc cctctgaacc tgtggactcg agtgactttg
     3181 caccaagaaa cagccggtat gatgcccaaa tatctgtatt tggggccaag ttccagaaga
     3241 aacttgaaga tgctaaagtt ttcacagtag ggtctggtgc tcttggctgt gagttcttga
     3301 aaaatttggc tctgatgggg gtttcttgtg gaagccaagg gaagctgaca gtgacagatg
     3361 atgatatcat tgagaagagt aacctcagtc gtcaatttct gttccgtgac tggaacattg
     3421 gacaggctaa atccacagtt gctgcttcgg ctgctgctgt tataaatcct cgcttcaaca
     3481 ttgaggccct gcaaaaccgt gttggcgcgg agactgagaa tgtatttgac gatgccttct
     3541 gggagaactt aactgttgtc gtcaatgcac tggataatgt taatgcgagg ctctatgttg
     3601 attcgaggtg cttgtatttc cagaagcctc tccttgagtc tggaactctt ggtacaaagt
     3661 gcaacacaca gtcggttatc ccacatctaa ctgaaaatta cggtgcctca agggacccac
     3721 cagagaaaca ggcccccatg tgtacagtgc attccttccc gcataacatt gatcactgtt
     3781 taacttgggc tcgctctgag tttgagggtc tgctggagaa aactcccgct gaagtgaatg
     3841 catatctttc tagcccagtt gagtacacta actcaatgat gagtgctggt gatgctcagg
     3901 cgagggacac attggagagg attgttgagt gccttgaaaa ggagaagtgt gaaaccttcc
     3961 aagactgctt aacctgggca cgactgaggt ttgaggatta ctttgtgaac cgtgtcaagc
     4021 aattgatata cacatttcct gaagatgctg cgactagtac cggagctcca ttctggtctg
     4081 ctccaaaaag attccctcgt ccactccagt actcgtcgtc cgacccaagt ctccttaact
     4141 ttatcacagc gactgctatt ttacgagcag agacatttgg gatcccgata cctgagtgga
     4201 ctaagaaccc aaaggaagca gcagaagctg ttgacagagt gatagtccca gactttgagc
     4261 caaggcaaga tgcaaagatt gtgactgatg agaaagctac cactttaacc actgcttcgg
     4321 tggacgacgc tgcagtcatc gacgatctca ttgctaagat cgaccagtgt aggcataact
     4381 tgtcgccaga cttcaggatg aaacctattc agttcgaaaa ggatgatgat acaaactatc
     4441 acatggatgt gatagcagga ctagccaaca tgagggcgag aaattacagc atccctgaag
     4501 tggacaagct gaaagcaaag ttcatagcag ggagaatcat cccagccatt gcgacttcaa
     4561 cagccatggc tactgggctg gtctgcctcg agctgtacaa ggtccttgat ggaggacata
     4621 aagtggaagc ttacagaaac acatttgcca acctggcact tccactattc tccatggctg
     4681 aaccacttcc accaaaggtg gtgaagcacc gtgacatggc ttggacagtt tgggacagat
     4741 gggttctcaa gggaaaccca actcttcgtg aggtgttgca gtggctggag gacaaagggc
     4801 ttagcgcata cagcatctct tgtggaagct gtctactgtt caacagtatg ttcacaaggc
     4861 acaaggagag gatggacaag aaggttgtgg atctcgctcg ggatgttgcg aaagtcgagt
     4921 taccgcctta tcgcaaccat cttgatgtag tggtggcttg tgaggatgaa gatgacaatg
     4981 acgtcgatat tcctctcgtc tctatctact tcaggtgagc ttactattaa ctttaagaag
     5041 gagatatacc atgtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     5101 ggactatgca ggattgtatc catatgacgt tccagattac gccactagag ctgcttaccc
     5161 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggattgta
     5221 tccatatgac gttccagatt acgcaatggc taataaccaa gcaagccttc ttctgcagaa
     5281 acagcttaaa gatctgtgta agaaacctgt tgatggtttc tctgctggcc tcgttgatga
     5341 gaagaacgtt ttccaatgga gcgtctctat tatgggtcct cctgatactt tgtatgaagg
     5401 tggctttttt aatgcaatta tgtcgtttcc ggagaattat ccagtttcac caccaactgt
     5461 gacgtttacc tcggagatgt ggcatcctaa tgtttattct gatggaaaag tttgcatttc
     5521 tattcttcat cctcctggtg atgatcctca tggatacgag cttgcctctg aacgttggac
     5581 ccctgtccat acggtagaaa gcattgtgtt gagtatcata tcgatgctct caggtcccaa
     5641 cgatgagtca ccagcgaatg tggaagctgc aaaggaatgg agagacaaca gagcagagtt
     5701 taggaagaaa gtgagtcgct gtgtgagaag atcgcaagag atgctatgag cttttacgct
     5761 tttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5821 cccggcatcc gcttacagac aagctgtgac cgggatgaga tccggctgct aacaaagccc
     5881 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg
     5941 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggattggcga
     6001 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     6061 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     6121 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     6181 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     6241 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     6301 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     6361 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     6421 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
     6481 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     6541 atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt tattcatatc
     6601 aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag aaaactcacc
     6661 gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga ctcgtccaac
     6721 atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg agaaatcacc
     6781 atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt tccagacttg
     6841 ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca aaccgttatt
     6901 cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag gacaattaca
     6961 aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa tattttcacc
     7021 tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg cagtggtgag
     7081 taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag gcataaattc
     7141 cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc tacctttgcc
     7201 atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga ttgtcgcacc
     7261 tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat ccatgttgga
     7321 atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa caccccttgt
     7381 attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct taacgtgagt
     7441 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     7501 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     7561 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     7621 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     7681 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     7741 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     7801 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     7861 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     7921 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     7981 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8041 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     8101 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     8161 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     8221 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     8281 tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta caatctgctc
     8341 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
     8401 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     8461 tccgcttaca gacaagctgt gaccgactcc gggagctgca tgtgtcagag gttttcaccg
     8521 tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat
     8581 tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat
     8641 gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat
     8701 gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg
     8761 atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt
     8821 aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag
     8881 cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag
     8941 atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg
     9001 aaacccaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
     9061 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
     9121 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc catgccggcg
     9181 ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg
     9241 agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga
     9301 aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg
     9361 ataaagaaca cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag
     9421 ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc
     9481 taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     9541 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gt
//
