LOCUS       sequence-205500-        9567 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9567)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9567)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-307
FEATURES             Location/Qualifiers
     source          1..9567
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     970..989
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1009..1086)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1292..1311
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1395..1414
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1395..1413
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1414..1438
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1492..1509
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5057..5146
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5159..5248
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5896..5914)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5910..5957
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5994..6449
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6081..6100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6291..6312
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6541..7356)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6541..7356)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7264..7283
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7348..7367
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7478..8066
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7967..7986
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8220..8237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8252..8391
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8317..8336)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8477..8499
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8493..8684)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9493..9567,1..1008))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggtttgcgta ttgggcgcca gggtggtttt tcttttcacc agtgacacgg gcaacagctg
       61 attgcccttc accgcctggc cctgagagag ttgcagcaag cggtccacgc tggtttgccc
      121 cagcaggcga aaatcctgtt tgatggtggt taacggcggg atataacatg agctgtcctc
      181 ggtatcgtcg tatcccacta ccgagatatc cgcaccaacg cgcagcccgg actcggtaat
      241 ggcgcgcatt gcgcccagcg ccatctgatc gttggcaacc agcatcgcag tgggaacgat
      301 gccctcattc agcatttgca tggtttgttg aaaaccggac atggcactcc agtcgccttc
      361 ccgttccgct atcggctgaa tttgattgcg agtgagatat ttatgccagc cagccagacg
      421 cagacgcgcc gagacagaac ttaatgggcc cgctaacagc gcgatttgct ggtgacccaa
      481 tgcgaccaga tgctccacgc ccagtcgcgt accgtcctca tgggagaaaa taatactgtt
      541 gatgggtgtc tggtcagaga catcaagaaa taacgccgga acattagtgc aggcagcttc
      601 cacagcaatg gcatcctggt catccagcgg atagttaatg atcagcccac tgacgcgttg
      661 cgcgagaaga ttgtgcaccg ccgctttaca ggcttcgacg ccgcttcgtt ctaccatcga
      721 caccaccacg ctggcaccca gttgatcggc gcgagattta atcgccgcga caatttgcga
      781 cggcgcgtgc agggccagac tggaggtggc aacgccaatc agcaacgact gtttgcccgc
      841 cagttgttgt gccacgcggt tgggaatgta attcagctcc gccatcgccg cttccacttt
      901 ttcccgcgtt ttcgcagaaa cgtggctggc ctggttcacc acgcgggaaa cggtctgata
      961 agagacaccg gcatactctg cgacatcgta taacgttact ggtttcacat tcaccaccct
     1021 gaattgactc tcttccgggc gctatcatgc cataccgcga aaggttttgc gccattcgat
     1081 ggtgtccggg atctcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta
     1141 gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc
     1201 ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga
     1261 gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa
     1321 ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct
     1381 cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata acaattcccc
     1441 tctagaaata attttgcctt aactttaaga aggagatata ccatgggcag ccaccaccac
     1501 caccaccaca gcggaatgca gatctttgtt aagactctca ccggaaagac aatcacccta
     1561 gaggtggaaa gctccgacac catcgacaac gttaaggcca agatccagga taaggagggc
     1621 attcctccgg atcagcagag gcttattttc gccggcaagc agctagagga tggccgtacg
     1681 ttggctgatt acaatatcca gaaggaatcc accctccact tggtcctcag gctccgtggt
     1741 ggttaagctt gcaattaact ttaagaagga gatatacaat gcttcacaag cgagctagtg
     1801 aagctaacga taagaacgat aataccatca tcggttccga tttagcatca tctaagaaac
     1861 gccgtatcga tttcactgaa tcttcgtctg ataagtcatc ttcgatttta gcttctggta
     1921 gtagcagggg ttttcacggc gatagcgtcg ttcagcaaat cgacatggct tttggtaatt
     1981 cgaaccgtca ggagattgat gaagatctgc acagtcgcca gctcgccgtc tatggtcgtg
     2041 agactatgag gcgtctcttt gcttccaatg ttctcatctc ggggatgcac ggtcttggtg
     2101 ccgagattgc caagaatctt atacttgctg gtgtgaagtc tgtgaccttg catgatgaaa
     2161 gagttgtaga gctatgggac ttatcaagca actttgtttt ctcggaggat gatgttggca
     2221 agaatagagc agatgcttct gttcagaagt tacaggatct taacaatgct gtggttgtct
     2281 ctagcttgac caaaagctta aacaaagagg atctttctgg cttccaggtt gttgtattct
     2341 ctgacataag catggaaaga gcaatcgaat ttgatgacta ttgtcacagc catcagcctc
     2401 ctatcgcttt tgtcaaggct gatgtcaggg gtcttttcgg ctctgtcttt tgtgattttg
     2461 ggcctgaatt tgcggttctt gatgttgatg gagaggaacc ccatacaggc atcattgcgt
     2521 ctatctctaa tgagaaccag gcatttattt cctgtgtcga cgatgagagg cttgagtttg
     2581 aagatggtga cctagttgtt ttctcagaag ttgagggtat gacagaattg aatgatggga
     2641 aaccaaggaa gataaaaagt acacggccat attcattcac ccttgatgag gacactacta
     2701 actatggaac atatgtgaag ggtggtattg tcactcaagt gaaacagcca aagttactga
     2761 atttcaagcc attgagggaa gcgcttaagg atccagggga ttttctattt agtgatttct
     2821 ctaagtttga tcggcctcca ctccttcact tagcattcca ggcacttgat cattttaaag
     2881 ctgaagctgg cagatttcct gttgctggct cagaagagga cgctcagaag cttatatcga
     2941 ttgccacagc catcaatacc gggcagggtg atttaaaggt ggagaatgtt gatcagaagc
     3001 ttctaagaca cttttccttt ggagccaagg ctgtcctgaa tcctatggct gcaatgtttg
     3061 gcggtattgt tggacaggag gttgttaaag cttgctctgg aaaatttcat cctctcttcc
     3121 agtttttcta ctttgattca gtggagtcac tcccctctga acctgtggac tcgagtgact
     3181 ttgcaccaag aaacagccgg tatgatgccc aaatatctgt atttggggcc aagttccaga
     3241 agaaacttga agatgctaaa gttttcacag tagggtctgg tgctcttggc tgtgagttct
     3301 tgaaaaattt ggctctgatg ggggtttctt gtggaagcca agggaagctg acagtgacag
     3361 atgatgatat cattgagaag agtaacctca gtcgtcaatt tctgttccgt gactggaaca
     3421 ttggacaggc taaatccaca gttgctgctt cggctgctgc tgttataaat cctcgcttca
     3481 acattgaggc cctgcaaaac cgtgttggcg cggagactga gaatgtattt gacgatgcct
     3541 tctgggagaa cttaactgtt gtcgtcaatg cactggataa tgttaatgcg aggctctatg
     3601 ttgattcgag gtgcttgtat ttccagaagc ctctccttga gtctggaact cttggtacaa
     3661 agtgcaacac acagtcggtt atcccacatc taactgaaaa ttacggtgcc tcaagggacc
     3721 caccagagaa acaggccccc atgtgtacag tgcattcctt cccgcataac attgatcact
     3781 gtttaacttg ggctcgctct gagtttgagg gtctgctgga gaaaactccc gctgaagtga
     3841 atgcatatct ttctagccca gttgagtaca ctaactcaat gatgagtgct ggtgatgctc
     3901 aggcgaggga cacattggag aggattgttg agtgccttga aaaggagaag tgtgaaacct
     3961 tccaagactg cttaacctgg gcacgactga ggtttgagga ttactttgtg aaccgtgtca
     4021 agcaattgat atacacattt cctgaagatg ctgcgactag taccggagct ccattctggt
     4081 ctgctccaaa aagattccct cgtccactcc agtactcgtc gtccgaccca agtctcctta
     4141 actttatcac agcgactgct attttacgag cagagacatt tgggatcccg atacctgagt
     4201 ggactaagaa cccaaaggaa gcagcagaag ctgttgacag agtgatagtc ccagactttg
     4261 agccaaggca agatgcaaag attgtgactg atgagaaagc taccacttta accactgctt
     4321 cggtggacga cgctgcagtc atcgacgatc tcattgctaa gatcgaccag tgtaggcata
     4381 acttgtcgcc agacttcagg atgaaaccta ttcagttcga aaaggatgat gatacaaact
     4441 atcacatgga tgtgatagca ggactagcca acatgagggc gagaaattac agcatccctg
     4501 aagtggacaa gctgaaagca aagttcatag cagggagaat catcccagcc attgcgactt
     4561 caacagccat ggctactggg ctggtctgcc tcgagctgta caaggtcctt gatggaggac
     4621 ataaagtgga agcttacaga aacacatttg ccaacctggc acttccacta ttctccatgg
     4681 ctgaaccact tccaccaaag gtggtgaagc accgtgacat ggcttggaca gtttgggaca
     4741 gatgggttct caagggaaac ccaactcttc gtgaggtgtt gcagtggctg gaggacaaag
     4801 ggcttagcgc atacagcatc tcttgtggaa gctgtctact gttcaacagt atgttcacaa
     4861 ggcacaagga gaggatggac aagaaggttg tggatctcgc tcgggatgtt gcgaaagtcg
     4921 agttaccgcc ttatcgcaac catcttgatg tagtggtggc ttgtgaggat gaagatgaca
     4981 atgacgtcga tattcctctc gtctctatct acttcaggtg agcttactat taactttaag
     5041 aaggagatat accatgtacc catacgatgt tcctgactat gcgggctatc cctatgacgt
     5101 cccggactat gcaggattgt atccatatga cgttccagat tacgccacta gagctgctta
     5161 cccatacgat gttcctgact atgcgggcta tccctatgac gtcccggact atgcaggatt
     5221 gtatccatat gacgttccag attacgcaat gatgtcaagt tctggtgctc cttcacgcaa
     5281 gactctgagt aagatcgcga ctaatcgtct acagaaagag cttgtggaat ggcagatgaa
     5341 tccacctact ggtttcaaac acaaagtcac tgataatctc caaagatgga taattgaagt
     5401 tattggagct ccaggaactc tatatgccaa cgatacttat cagcttcaag ttgattttcc
     5461 agaacattat cctatggaat cgccacaagt gatttttctt catccagctc ctctgcatcc
     5521 tcacatttac agcaatgggc atatttgtct agatattttg tatgattcgt ggtcaccagc
     5581 catgacggtg agttctatct gcattagcat cctctccatg ctctcaagct cgactgaaaa
     5641 gcaacgacca accgataatg accgatatgt gaagaattgt aagaacggaa gatctccaaa
     5701 ggaaacccga tggtggttcc acgacgataa agtataagct tttacgcttt taagccagcc
     5761 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5821 ttacagacaa gctgtgaccg ggatgagatc cggctgctaa caaagcccga aaggaagctg
     5881 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     5941 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
     6001 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6061 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6121 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6181 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6241 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6301 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6361 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6421 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     6481 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     6541 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     6601 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     6661 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     6721 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     6781 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     6841 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     6901 cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     6961 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     7021 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     7081 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     7141 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     7201 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     7261 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     7321 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     7381 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
     7441 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7501 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7561 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7621 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7681 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7741 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7801 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7861 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7921 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     7981 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8041 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8101 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8161 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8221 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     8281 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
     8341 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     8401 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8461 caagctgtga ccgactccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8521 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     8581 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     8641 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     8701 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     8761 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     8821 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     8881 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     8941 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa acccaagacc
     9001 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     9061 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     9121 aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc
     9181 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     9241 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     9301 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaacaca
     9361 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     9421 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     9481 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9541 tgaatcggcc aacgcgcggg gagaggc
//
