LOCUS       sequence-205502-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-309
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     103..122
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        103..121
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    122..146
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             200..217
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3765..3854
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3867..3956
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4601..4619)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4615..4662
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4699..5154
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4786..4805)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4996..5017
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5246..6061)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5246..6061)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5969..5988
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6053..6072
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6183..6771
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6672..6691
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6925..6942
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6957..7096
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7022..7041)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7182..7204
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7198..7389)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8198..9280)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9242..9261
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9281..9358)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     join(9564,1..19)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
       61 atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa attaatacga ctcactatag
      121 gggaattgtg agcggataac aattcccctc tagaaataat tttgccttaa ctttaagaag
      181 gagatatacc atgggcagcc accaccacca ccaccacagc ggaatgcaga tctttgttaa
      241 gactctcacc ggaaagacaa tcaccctaga ggtggaaagc tccgacacca tcgacaacgt
      301 taaggccaag atccaggata aggagggcat tcctccggat cagcagaggc ttattttcgc
      361 cggcaagcag ctagaggatg gccgtacgtt ggctgattac aatatccaga aggaatccac
      421 cctccacttg gtcctcaggc tccgtggtgg ttaagcttgc aattaacttt aagaaggaga
      481 tatacaatgc ttcacaagcg agctagtgaa gctaacgata agaacgataa taccatcatc
      541 ggttccgatt tagcatcatc taagaaacgc cgtatcgatt tcactgaatc ttcgtctgat
      601 aagtcatctt cgattttagc ttctggtagt agcaggggtt ttcacggcga tagcgtcgtt
      661 cagcaaatcg acatggcttt tggtaattcg aaccgtcagg agattgatga agatctgcac
      721 agtcgccagc tcgccgtcta tggtcgtgag actatgaggc gtctctttgc ttccaatgtt
      781 ctcatctcgg ggatgcacgg tcttggtgcc gagattgcca agaatcttat acttgctggt
      841 gtgaagtctg tgaccttgca tgatgaaaga gttgtagagc tatgggactt atcaagcaac
      901 tttgttttct cggaggatga tgttggcaag aatagagcag atgcttctgt tcagaagtta
      961 caggatctta acaatgctgt ggttgtctct agcttgacca aaagcttaaa caaagaggat
     1021 ctttctggct tccaggttgt tgtattctct gacataagca tggaaagagc aatcgaattt
     1081 gatgactatt gtcacagcca tcagcctcct atcgcttttg tcaaggctga tgtcaggggt
     1141 cttttcggct ctgtcttttg tgattttggg cctgaatttg cggttcttga tgttgatgga
     1201 gaggaacccc atacaggcat cattgcgtct atctctaatg agaaccaggc atttatttcc
     1261 tgtgtcgacg atgagaggct tgagtttgaa gatggtgacc tagttgtttt ctcagaagtt
     1321 gagggtatga cagaattgaa tgatgggaaa ccaaggaaga taaaaagtac acggccatat
     1381 tcattcaccc ttgatgagga cactactaac tatggaacat atgtgaaggg tggtattgtc
     1441 actcaagtga aacagccaaa gttactgaat ttcaagccat tgagggaagc gcttaaggat
     1501 ccaggggatt ttctatttag tgatttctct aagtttgatc ggcctccact ccttcactta
     1561 gcattccagg cacttgatca ttttaaagct gaagctggca gatttcctgt tgctggctca
     1621 gaagaggacg ctcagaagct tatatcgatt gccacagcca tcaataccgg gcagggtgat
     1681 ttaaaggtgg agaatgttga tcagaagctt ctaagacact tttcctttgg agccaaggct
     1741 gtcctgaatc ctatggctgc aatgtttggc ggtattgttg gacaggaggt tgttaaagct
     1801 tgctctggaa aatttcatcc tctcttccag tttttctact ttgattcagt ggagtcactc
     1861 ccctctgaac ctgtggactc gagtgacttt gcaccaagaa acagccggta tgatgcccaa
     1921 atatctgtat ttggggccaa gttccagaag aaacttgaag atgctaaagt tttcacagta
     1981 gggtctggtg ctcttggctg tgagttcttg aaaaatttgg ctctgatggg ggtttcttgt
     2041 ggaagccaag ggaagctgac agtgacagat gatgatatca ttgagaagag taacctcagt
     2101 cgtcaatttc tgttccgtga ctggaacatt ggacaggcta aatccacagt tgctgcttcg
     2161 gctgctgctg ttataaatcc tcgcttcaac attgaggccc tgcaaaaccg tgttggcgcg
     2221 gagactgaga atgtatttga cgatgccttc tgggagaact taactgttgt cgtcaatgca
     2281 ctggataatg ttaatgcgag gctctatgtt gattcgaggt gcttgtattt ccagaagcct
     2341 ctccttgagt ctggaactct tggtacaaag tgcaacacac agtcggttat cccacatcta
     2401 actgaaaatt acggtgcctc aagggaccca ccagagaaac aggcccccat gtgtacagtg
     2461 cattccttcc cgcataacat tgatcactgt ttaacttggg ctcgctctga gtttgagggt
     2521 ctgctggaga aaactcccgc tgaagtgaat gcatatcttt ctagcccagt tgagtacact
     2581 aactcaatga tgagtgctgg tgatgctcag gcgagggaca cattggagag gattgttgag
     2641 tgccttgaaa aggagaagtg tgaaaccttc caagactgct taacctgggc acgactgagg
     2701 tttgaggatt actttgtgaa ccgtgtcaag caattgatat acacatttcc tgaagatgct
     2761 gcgactagta ccggagctcc attctggtct gctccaaaaa gattccctcg tccactccag
     2821 tactcgtcgt ccgacccaag tctccttaac tttatcacag cgactgctat tttacgagca
     2881 gagacatttg ggatcccgat acctgagtgg actaagaacc caaaggaagc agcagaagct
     2941 gttgacagag tgatagtccc agactttgag ccaaggcaag atgcaaagat tgtgactgat
     3001 gagaaagcta ccactttaac cactgcttcg gtggacgacg ctgcagtcat cgacgatctc
     3061 attgctaaga tcgaccagtg taggcataac ttgtcgccag acttcaggat gaaacctatt
     3121 cagttcgaaa aggatgatga tacaaactat cacatggatg tgatagcagg actagccaac
     3181 atgagggcga gaaattacag catccctgaa gtggacaagc tgaaagcaaa gttcatagca
     3241 gggagaatca tcccagccat tgcgacttca acagccatgg ctactgggct ggtctgcctc
     3301 gagctgtaca aggtccttga tggaggacat aaagtggaag cttacagaaa cacatttgcc
     3361 aacctggcac ttccactatt ctccatggct gaaccacttc caccaaaggt ggtgaagcac
     3421 cgtgacatgg cttggacagt ttgggacaga tgggttctca agggaaaccc aactcttcgt
     3481 gaggtgttgc agtggctgga ggacaaaggg cttagcgcat acagcatctc ttgtggaagc
     3541 tgtctactgt tcaacagtat gttcacaagg cacaaggaga ggatggacaa gaaggttgtg
     3601 gatctcgctc gggatgttgc gaaagtcgag ttaccgcctt atcgcaacca tcttgatgta
     3661 gtggtggctt gtgaggatga agatgacaat gacgtcgata ttcctctcgt ctctatctac
     3721 ttcaggtgag cttactatta actttaagaa ggagatatac catgtaccca tacgatgttc
     3781 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggattgtat ccatatgacg
     3841 ttccagatta cgccactaga gctgcttacc catacgatgt tcctgactat gcgggctatc
     3901 cctatgacgt cccggactat gcaggattgt atccatatga cgttccagat tacgcaatga
     3961 caagctcttc atctccttca cgcaaggcgt taagcaagat cgcgtgtaat aggttacaaa
     4021 aggagttatc agagtggcaa gtaaatccac cgactgggtt taagcacaga gttactgata
     4081 atcttcaaaa atgggtaatt gaggtaacag gagcacctgg tacgctttac gctaatgaaa
     4141 cttataatct tcaggttgag tttcctcaac attatcctat ggaagctcct caggtgattt
     4201 ttgttccacc agcaccgtta catcctcata tttacagcaa tggacatatt tgtttagata
     4261 ttctgtatga ctcatggtca ccagcaatga cagtgagttc agtctgcatc agcatcctct
     4321 caatgctatc aagttccccc gaaaagcaac gcccgactga taacgatcga tatgtgaaga
     4381 actgcaagaa cgggaggtca cctaaagaga caaggtggtg gttccatgac gacaaggttt
     4441 aagcttttac gcttttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
     4501 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgggatg agatccggct
     4561 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
     4621 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
     4681 tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4741 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4801 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4861 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4921 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4981 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     5041 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5101 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     5161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     5221 tatgtatccg ctcatgaatt aattcttaga aaaactcatc gagcatcaaa tgaaactgca
     5281 atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc tgtaatgaag
     5341 gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg tctgcgattc
     5401 cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata aggttatcaa
     5461 gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagt ttatgcattt
     5521 ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa
     5581 ccaaaccgtt attcattcgt gattgcgcct gagcgagtcg aaatacgcga tcgctgttaa
     5641 aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa
     5701 caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga
     5761 tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa
     5821 gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa
     5881 cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat
     5941 agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag
     6001 catccatgtt ggaatttaat cgcggcctag agcaagacgt ttcccgttga atatggctca
     6061 taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat gaccaaaatc
     6121 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     6181 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     6241 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     6301 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     6361 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     6421 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     6481 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     6541 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     6601 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     6661 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     6721 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     6781 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     6841 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     6901 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg
     6961 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcaatgg tgcactctca
     7021 gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga
     7081 ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     7141 tctgctcccg gcatccgctt acagacaagc tgtgaccgac tccgggagct gcatgtgtca
     7201 gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg
     7261 gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc
     7321 cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg
     7381 tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat
     7441 gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga
     7501 acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca
     7561 gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca
     7621 tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact
     7681 ttacgaaaca cggaaaccca agaccattca tgttgttgct caggtcgcag acgttttgca
     7741 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca
     7801 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggggc
     7861 cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac
     7921 gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt
     7981 cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc
     8041 tacgagttgc atgataaaga acacagtcat aagtgcggcg acgatagtca tgccccgcgc
     8101 ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgag atcccggtgc
     8161 ctaatgagtg agctaactta cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     8221 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     8281 tattgggcgc cagggtggtt tttcttttca ccagtgacac gggcaacagc tgattgccct
     8341 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
     8401 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtcc tcggtatcgt
     8461 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
     8521 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
     8581 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
     8641 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
     8701 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
     8761 gatgctccac gcccagtcgc gtaccgtcct catgggagaa aataatactg ttgatgggtg
     8821 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
     8881 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
     8941 gattgtgcac cgccgcttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
     9001 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
     9061 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
     9121 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
     9181 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
     9241 cggcatactc tgcgacatcg tataacgtta ctggtttcac attcaccacc ctgaattgac
     9301 tctcttccgg gcgctatcat gccataccgc gaaaggtttt gcgccattcg atggtgtccg
     9361 ggatctcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg
     9421 aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt
     9481 cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag
     9541 tggcgagccc gatcttcccc atcg
//
