LOCUS       sequence-205503-        9624 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9624)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-310
FEATURES             Location/Qualifiers
     source          1..9624
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     564..583
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(603..680)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     886..905
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     989..1008
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        989..1007
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1008..1032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1086..1103
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4651..4740
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4753..4842
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5547..5565)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5561..5608
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5645..6100
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5732..5751)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5942..5963
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6192..7007)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6192..7007)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6915..6934
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6999..7018
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7129..7717
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7618..7637
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7871..7888
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7903..8042
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7968..7987)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8128..8150
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8144..8335)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9144..9624,1..602))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt
       61 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ctcatgggag
      121 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta
      181 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc
      241 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt
      301 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc
      361 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac
      421 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc
      481 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg
      541 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc
      601 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt
      661 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag
      721 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg
      781 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa
      841 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat
      901 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta
      961 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg
     1021 gataacaatt cccctctaga aataattttg ccttaacttt aagaaggaga tataccatgg
     1081 gcagccacca ccaccaccac cacagcggaa tgcagatctt tgttaagact ctcaccggaa
     1141 agacaatcac cctagaggtg gaaagctccg acaccatcga caacgttaag gccaagatcc
     1201 aggataagga gggcattcct ccggatcagc agaggcttat tttcgccggc aagcagctag
     1261 aggatggccg tacgttggct gattacaata tccagaagga atccaccctc cacttggtcc
     1321 tcaggctccg tggtggttaa gcttgcaatt aactttaaga aggagatata caatgcttca
     1381 caagcgagct agtgaagcta acgataagaa cgataatacc atcatcggtt ccgatttagc
     1441 atcatctaag aaacgccgta tcgatttcac tgaatcttcg tctgataagt catcttcgat
     1501 tttagcttct ggtagtagca ggggttttca cggcgatagc gtcgttcagc aaatcgacat
     1561 ggcttttggt aattcgaacc gtcaggagat tgatgaagat ctgcacagtc gccagctcgc
     1621 cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc aatgttctca tctcggggat
     1681 gcacggtctt ggtgccgaga ttgccaagaa tcttatactt gctggtgtga agtctgtgac
     1741 cttgcatgat gaaagagttg tagagctatg ggacttatca agcaactttg ttttctcgga
     1801 ggatgatgtt ggcaagaata gagcagatgc ttctgttcag aagttacagg atcttaacaa
     1861 tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa gaggatcttt ctggcttcca
     1921 ggttgttgta ttctctgaca taagcatgga aagagcaatc gaatttgatg actattgtca
     1981 cagccatcag cctcctatcg cttttgtcaa ggctgatgtc aggggtcttt tcggctctgt
     2041 cttttgtgat tttgggcctg aatttgcggt tcttgatgtt gatggagagg aaccccatac
     2101 aggcatcatt gcgtctatct ctaatgagaa ccaggcattt atttcctgtg tcgacgatga
     2161 gaggcttgag tttgaagatg gtgacctagt tgttttctca gaagttgagg gtatgacaga
     2221 attgaatgat gggaaaccaa ggaagataaa aagtacacgg ccatattcat tcacccttga
     2281 tgaggacact actaactatg gaacatatgt gaagggtggt attgtcactc aagtgaaaca
     2341 gccaaagtta ctgaatttca agccattgag ggaagcgctt aaggatccag gggattttct
     2401 atttagtgat ttctctaagt ttgatcggcc tccactcctt cacttagcat tccaggcact
     2461 tgatcatttt aaagctgaag ctggcagatt tcctgttgct ggctcagaag aggacgctca
     2521 gaagcttata tcgattgcca cagccatcaa taccgggcag ggtgatttaa aggtggagaa
     2581 tgttgatcag aagcttctaa gacacttttc ctttggagcc aaggctgtcc tgaatcctat
     2641 ggctgcaatg tttggcggta ttgttggaca ggaggttgtt aaagcttgct ctggaaaatt
     2701 tcatcctctc ttccagtttt tctactttga ttcagtggag tcactcccct ctgaacctgt
     2761 ggactcgagt gactttgcac caagaaacag ccggtatgat gcccaaatat ctgtatttgg
     2821 ggccaagttc cagaagaaac ttgaagatgc taaagttttc acagtagggt ctggtgctct
     2881 tggctgtgag ttcttgaaaa atttggctct gatgggggtt tcttgtggaa gccaagggaa
     2941 gctgacagtg acagatgatg atatcattga gaagagtaac ctcagtcgtc aatttctgtt
     3001 ccgtgactgg aacattggac aggctaaatc cacagttgct gcttcggctg ctgctgttat
     3061 aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt ggcgcggaga ctgagaatgt
     3121 atttgacgat gccttctggg agaacttaac tgttgtcgtc aatgcactgg ataatgttaa
     3181 tgcgaggctc tatgttgatt cgaggtgctt gtatttccag aagcctctcc ttgagtctgg
     3241 aactcttggt acaaagtgca acacacagtc ggttatccca catctaactg aaaattacgg
     3301 tgcctcaagg gacccaccag agaaacaggc ccccatgtgt acagtgcatt ccttcccgca
     3361 taacattgat cactgtttaa cttgggctcg ctctgagttt gagggtctgc tggagaaaac
     3421 tcccgctgaa gtgaatgcat atctttctag cccagttgag tacactaact caatgatgag
     3481 tgctggtgat gctcaggcga gggacacatt ggagaggatt gttgagtgcc ttgaaaagga
     3541 gaagtgtgaa accttccaag actgcttaac ctgggcacga ctgaggtttg aggattactt
     3601 tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa gatgctgcga ctagtaccgg
     3661 agctccattc tggtctgctc caaaaagatt ccctcgtcca ctccagtact cgtcgtccga
     3721 cccaagtctc cttaacttta tcacagcgac tgctatttta cgagcagaga catttgggat
     3781 cccgatacct gagtggacta agaacccaaa ggaagcagca gaagctgttg acagagtgat
     3841 agtcccagac tttgagccaa ggcaagatgc aaagattgtg actgatgaga aagctaccac
     3901 tttaaccact gcttcggtgg acgacgctgc agtcatcgac gatctcattg ctaagatcga
     3961 ccagtgtagg cataacttgt cgccagactt caggatgaaa cctattcagt tcgaaaagga
     4021 tgatgataca aactatcaca tggatgtgat agcaggacta gccaacatga gggcgagaaa
     4081 ttacagcatc cctgaagtgg acaagctgaa agcaaagttc atagcaggga gaatcatccc
     4141 agccattgcg acttcaacag ccatggctac tgggctggtc tgcctcgagc tgtacaaggt
     4201 ccttgatgga ggacataaag tggaagctta cagaaacaca tttgccaacc tggcacttcc
     4261 actattctcc atggctgaac cacttccacc aaaggtggtg aagcaccgtg acatggcttg
     4321 gacagtttgg gacagatggg ttctcaaggg aaacccaact cttcgtgagg tgttgcagtg
     4381 gctggaggac aaagggctta gcgcatacag catctcttgt ggaagctgtc tactgttcaa
     4441 cagtatgttc acaaggcaca aggagaggat ggacaagaag gttgtggatc tcgctcggga
     4501 tgttgcgaaa gtcgagttac cgccttatcg caaccatctt gatgtagtgg tggcttgtga
     4561 ggatgaagat gacaatgacg tcgatattcc tctcgtctct atctacttca ggtgagctta
     4621 ctattaactt taagaaggag atataccatg tacccatacg atgttcctga ctatgcgggc
     4681 tatccctatg acgtcccgga ctatgcagga ttgtatccat atgacgttcc agattacgcc
     4741 actagagctg cttacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg
     4801 gactatgcag gattgtatcc atatgacgtt ccagattacg caatggcgac ggttaatggg
     4861 tacacaggga atactccggc ggcgactaca ccggcagcca ccggatcaaa gcaatctgct
     4921 cctccgacta agaccgtcga tagccactcc gttctaaaaa ggctgcaatc tgaactaatg
     4981 ggattgatga tgggagctga tccggggata tctgcgtttc cagaggagga caacatattt
     5041 tgctggaaag gaacaattac aggaagcaaa gatactgtgt tcgaaggaac tgagtacaga
     5101 ctctcactca ctttctctaa cgattatcct tttaagtctc ccaaagttaa gtttgagaca
     5161 tgctgcttcc accccaatgt ggatctctat ggcaatattt gcttggacat tcttcaggat
     5221 aaatggtcat ctgcttatga tgtgaggacg atattactct caattcaaag ccttctcgga
     5281 gaaccgaaca tcagctcacc attgaacaat caagcggctc agctttggag caatcaagaa
     5341 gagtacagga agatggttga gaagctctac aagcctttaa acgcatgagc ttttacgctt
     5401 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     5461 ccggcatccg cttacagaca agctgtgacc gggatgagat ccggctgcta acaaagcccg
     5521 aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc
     5581 ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gattggcgaa
     5641 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     5701 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     5761 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     5821 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     5881 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     5941 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     6001 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     6061 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     6121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     6181 tgaattaatt cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca
     6241 ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg
     6301 aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca
     6361 tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca
     6421 tgagtgacga ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt
     6481 tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc
     6541 attcgtgatt gcgcctgagc gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa
     6601 acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct
     6661 gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt
     6721 aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc
     6781 gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca
     6841 tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct
     6901 gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa
     6961 tttaatcgcg gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta
     7021 ttactgttta tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt
     7081 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7141 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7201 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7261 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7321 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7381 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7441 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7501 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7561 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7681 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7741 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7801 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7861 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
     7921 ccttacgcat ctgtgcggta tttcacaccg caatggtgca ctctcagtac aatctgctct
     7981 gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     8041 cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     8101 ccgcttacag acaagctgtg accgactccg ggagctgcat gtgtcagagg ttttcaccgt
     8161 catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt
     8221 cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg
     8281 tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg
     8341 cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga
     8401 tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta
     8461 aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc
     8521 gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga
     8581 tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga
     8641 aacccaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc
     8701 acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta
     8761 gccgggtcct caacgacagg agcacgatca tgcgcacccg tggggccgcc atgccggcga
     8821 taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga
     8881 gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa
     8941 agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga
     9001 taaagaacac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc
     9061 tgactgggtt gaaggctctc aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct
     9121 aacttacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     9181 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg
     9241 gtggtttttc ttttcaccag tgacacgggc aacagctgat tgcccttcac cgcctggccc
     9301 tgagagagtt gcagcaagcg gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg
     9361 atggtggtta acggcgggat ataacatgag ctgtcctcgg tatcgtcgta tcccactacc
     9421 gagatatccg caccaacgcg cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc
     9481 atctgatcgt tggcaaccag catcgcagtg ggaacgatgc cctcattcag catttgcatg
     9541 gtttgttgaa aaccggacat ggcactccag tcgccttccc gttccgctat cggctgaatt
     9601 tgattgcgag tgagatattt atgc
//
