LOCUS       sequence-205504-       16474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that 
            targets the CACTA1 promoter in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16474)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 16474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF2CACTA1_3xFLAG_TET1cd
FEATURES             Location/Qualifiers
     source          1..16474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      408..602
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(668..1741)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2170..2799)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4100..4124
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4312..4334
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4326..4343
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4327..4343
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6929..7000
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    7040..7064
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(9267..9291)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(10103..10119)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10103..10119)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10116..10138)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10127..10143
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10151..10157,10158..10175,10176..10181))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10196..10217
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10408..11084
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     11021..11041
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11129..11680
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    11687..11861
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    11939..11963
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12388..13182
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      14607..15195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15096..15115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     15349..15366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    15381..15521
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(15447..15466)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15607..15629
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(15683..16474)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
       61 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
      121 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      181 gcgttcggtc aaggttctgg accagttgcg tgagcgcatg gcgccggcgg tcgagtggcg
      241 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
      301 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
      361 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
      421 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
      481 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
      541 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
      601 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
      661 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
      721 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
      781 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
      841 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
      901 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
      961 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     1021 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     1081 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     1141 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     1201 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     1261 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     1321 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     1381 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     1441 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     1501 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     1561 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     1621 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     1681 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
     1741 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
     1801 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     1861 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
     1921 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
     1981 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
     2041 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
     2101 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
     2161 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
     2221 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
     2281 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
     2341 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
     2401 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
     2461 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
     2521 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
     2581 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
     2641 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
     2701 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
     2761 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
     2821 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
     2881 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
     2941 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
     3001 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
     3061 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
     3121 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
     3181 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
     3241 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
     3301 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
     3361 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
     3421 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
     3481 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
     3541 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
     3601 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
     3661 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
     3721 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
     3781 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
     3841 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
     3901 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
     3961 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
     4021 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
     4081 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
     4141 actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca
     4201 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4261 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     4321 gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac tctagaggat
     4381 ccccgggtac cgggcccccc ctcgaggcgc gagtctagct caacagagct tttaacccaa
     4441 attggtacaa tagaatacaa ctttagatca taattctcaa aagaaagaga ttccttagct
     4501 attctatctg ccactccatt tccttctcgg cttgtatgca caagcataaa atcctcaaac
     4561 ttgctaagta gatactttat gtcttggata attggattga gacttgacaa gcataacttt
     4621 catgtaacca aagacacaag ttgctgagaa tccacctcaa aaatgatctt cctataattg
     4681 aatcgggata atgacagcac agcccatcta agagcctcca cttctacttc cagcacgctt
     4741 cttactttta ccacagctct tgcacctaac cataacacct tccctgtatg atcgcgaagc
     4801 acccacccta agccacattt taatccttct gttggccatg ccccatcaaa gttgcactta
     4861 acccaagatt gtggtggagc ttcccatgtt tctcgtctgt cccgacggtg ttgtggttgg
     4921 tgctttcctt acattctgag cctctttcct tctaatccac tcatctgcat cttcttgtgt
     4981 ccttactaat acctcattgg ttccaaattc cctcccttta agcaccagct cgtttctgtt
     5041 cttccacagc ctcccaagta tccaagggac taaagcctcc acattcttca gatcaggata
     5101 ttcttgttta agatgttgaa ctctatggag gtttgtatga actgatgatc taggaccgga
     5161 taagttccct tcttcatagc gaacttattc aaagaatgtt ttgtgtatca ttcttgttac
     5221 attgttatta atgaaaaaat attattggtc attgggctga acacgagtgt taaatatgga
     5281 ccaggcccca aataagatcc attgatatat gaattaaata acaagaataa atcgagtcac
     5341 caaaccactt gcctttttta acgagacttg ttcaccaact tgatacaaaa gtcattatcc
     5401 tatgcaaatc aataatcata caaaaatatc caataacact aaaaaattaa aagaaatgga
     5461 taatttcaca atatgttata cgataaagaa gttacttttc caagaaattc actgatttta
     5521 taagcccact tgcattagat aaatggcaaa aaaaaacaaa aaggaaaaga aataaagcac
     5581 gaagaattct agaaaatacg aaatacgctt caatgcagtg ggacccacgg ttcaattatt
     5641 gccaattttc agctccaccg tatatttaaa aaataaaacg ataatgctaa aaaaatataa
     5701 atcgtaacga tcgttaaatc tcaacggctg gatcttatga cgaccgttag aaattgtggt
     5761 tgtcgacgag tcagtaataa acggcgtcaa agtggttgca gccggcacac acgagtcgtg
     5821 tttatcaact caaagcacaa atacttttcc tcaacctaaa aataaggcaa ttagccaaaa
     5881 acaactttgc gtgtaaacaa cgctcaatac acgtgtcatt ttattattag ctattgcttc
     5941 accgccttag ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt cttctataaa
     6001 acaataccca aagagctctt cttcttcaca attcagattt caatttctca aaatcttaaa
     6061 aactttctct caattctctc taccgtgatc aaggtaaatt tctgtgttcc ttattctctc
     6121 aaaatcttcg attttgtttt cgttcgatcc caatttcgta tatgttcttt ggtttagatt
     6181 ctgttaatct tagatcgaag acgattttct gggtttgatc gttagatatc atcttaattc
     6241 tcgattaggg tttcatagat atcatccgat ttgttcaaat aatttgagtt ttgtcgaata
     6301 attactcttc gatttgtgat ttctatctag atctggtgtt agtttctagt ttgtgcgatc
     6361 gaatttgtcg attaatctga gtttttctga ttaacagatg gttgagaagc cctataagtg
     6421 ccccgagtgt gggaagtctt tcagcaggga agataacctc cacactcatc aaagaacgca
     6481 tacgggagaa aagccttaca aatgtccgga atgtgggaaa tcattcagcc aatcaagcaa
     6541 tctcgtgaga catcaacgta cacatacagg ggaaaaacca tataagtgcc ccgaatgcgg
     6601 taagtcattt tcacaccgta ctactctgac aaaccatcaa cgtacccata ctggggagaa
     6661 gccttataaa tgtcctgagt gtgggaagag cttctctcag agggcgcatt tggagcgtca
     6721 ccagaggaca cataccggcg aaaaaccgta caaatgtcct gaatgtggaa agtcattctc
     6781 acgtagtgac aatctcgtca ggcaccagcg tactcatact ggagagaaac cctacaagtg
     6841 tcctgaatgt ggtaagtcct tcagtcaatc ctccagccta gtgagacatc aaaggacgca
     6901 taccgggaac cgcgccaagc ttggtaccga ctacaaagac gatgacgaca aagactacaa
     6961 agacgatgac gacaaagact acaaagacga tgacgacaaa ggatcccttg aggttctttt
     7021 cggcgcgcca agctatcaaa caagtttgta caaaaaagca ggctccgcgg ccgccccctt
     7081 caccatggga tccctgccca cctgcagctg tcttgatcga gttatacaaa aagacaaagg
     7141 cccatattat acacaccttg gggcaggacc aagtgttgct gctgtcaggg aaatcatgga
     7201 gaataggtat ggtcaaaaag gaaacgcaat aaggatagaa atagtagtgt acaccggtaa
     7261 agaagggaaa agctctcatg ggtgtccaat tgctaagtgg gttttaagaa gaagcagtga
     7321 tgaagaaaaa gttctttgtt tggtccggca gcgtacaggc caccactgtc caactgctgt
     7381 gatggtggtg ctcatcatgg tgtgggatgg catccctctt ccaatggccg accggctata
     7441 cacagagctc acagagaatc taaagtcata caatgggcac cctaccgaca gaagatgcac
     7501 cctcaatgaa aatcgtacct gtacatgtca aggaattgat ccagagactt gtggagcttc
     7561 attctctttt ggctgttcat ggagtatgta ctttaatggc tgtaagtttg gtagaagccc
     7621 aagccccaga agatttagaa ttgatccaag ctctccctta catgaaaaaa accttgaaga
     7681 taacttacag agtttggcta cacgattagc tccaatttat aagcagtatg ctccagtagc
     7741 ttaccaaaat caggtggaat atgaaaatgt tgcccgagaa tgtcggcttg gcagcaagga
     7801 aggtcgaccc ttctctgggg tcactgcttg cctggacttc tgtgctcatc cccacaggga
     7861 cattcacaac atgaataatg gaagcactgt ggtttgtacc ttaactcgag aagataaccg
     7921 ctctttgggt gttattcctc aagatgagca gctccatgtg ctacctcttt ataagctttc
     7981 agacacagat gagtttggct ccaaggaagg aatggaagcc aagatcaaat ctggggccat
     8041 cgaggtcctg gcaccccgcc gcaaaaaaag aacgtgtttc actcagcctg ttccccgttc
     8101 tggaaagaag agggctgcga tgatgacaga ggttcttgca cataagataa gggcagtgga
     8161 aaagaaacct attccccgaa tcaagcggaa gaataactca acaacaacaa acaacagtaa
     8221 gccttcgtca ctgccaacct tagggagtaa cactgagacc gtgcaacctg aagtaaaaag
     8281 tgaaaccgaa ccccatttta tcttaaaaag ttcagacaac actaaaactt attcgctgat
     8341 gccatccgct cctcacccag tgaaagaggc atctccaggc ttctcctggt ccccgaagac
     8401 tgcttcagcc acaccagctc cactgaagaa tgacgcaaca gcctcatgcg ggttttcaga
     8461 aagaagcagc actccccact gtacgatgcc ttcgggaaga ctcagtggtg ccaatgctgc
     8521 agctgctgat ggccctggca tttcacagct tggcgaagtg gctcctctcc ccaccctgtc
     8581 tgctcctgtg atggagcccc tcattaattc tgagccttcc actggtgtga ctgagccgct
     8641 aacgcctcat cagccaaacc accagccctc cttcctcacc tctcctcaag accttgcctc
     8701 ttctccaatg gaagaagatg agcagcattc tgaagcagat gagcctccat cagacgaacc
     8761 cctatctgat gaccccctgt cacctgctga ggagaaattg ccccacattg atgagtattg
     8821 gtcagacagt gagcacatct ttttggatgc aaatattggt ggggtggcca tcgcacctgc
     8881 tcacggctcg gttttgattg agtgtgcccg gcgagagctg cacgctacca ctcctgttga
     8941 gcaccccaac cgtaatcatc caacccgcct ctcccttgtc ttttaccagc acaaaaacct
     9001 aaataagccc caacatggtt ttgaactaaa caagattaag tttgaggcta aagaagctaa
     9061 gaataagaaa atgaaggcct cagagcaaaa agaccaggca gctaatgaag gtccagaaca
     9121 gtcctctgaa gtaaatgaat tgaaccaaat tccttctcat aaagcattaa cattaaccca
     9181 tgacaatgtt gtcaccgtgt ccccttatgc tctcacacac gttgcggggc cctataacca
     9241 ttgggtctga aagggtgggc gcgccgaccc agctttcttg tacaaagtgg ttcgataatt
     9301 cttaattaac tagttctaga gcggccgcca ccgcggtgga gctcctaggt gagtctagag
     9361 agttaattaa gacccgggac tagtccctag agtcctgctt taatgagata tgcgagacgc
     9421 ctatgatcgc atgatatttg ctttcaattc tgttgtgcac gttgtaaaaa acctgagcat
     9481 gtgtagctca gatccttacc gccggtttcg gttcattcta atgaatatat cacccgttac
     9541 tatcgtattt ttatgaataa tattctccgt tcaatttact gattgtaccc tactacttat
     9601 atgtacaata ttaaaatgaa aacaatatat tgtgctgaat aggtttatag cgacatctat
     9661 gatagagcgc cacaataaca aacaattgcg ttttattatt acaaatccaa ttttaaaaaa
     9721 agcggcagaa ccggtcaaac ctaaaagact gattacataa atcttattca aatttcaaaa
     9781 gtgccccagg ggctagtatc tacgacacac cgagcggcga actaataacg ctcactgaag
     9841 ggaactccgg ttccccgccg gcgcgcatgg gtgagattcc ttgaagttga gtattggccg
     9901 tccgctctac cgaaagttac gggcaccatt caacccggtc cagcacggcg gccgggtaac
     9961 cgacttgctg ccccgagaat tatgcagcat ttttttggtg tatgtgggcc ccaaatgaag
    10021 tgcaggtcaa accttgacag tgacgacaaa tcgttgggcg ggtccagggc gaattttgcg
    10081 acgagctcga attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca
    10141 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
    10201 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
    10261 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct
    10321 agagcagctt gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga
    10381 tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa
    10441 cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga
    10501 aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc
    10561 tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga
    10621 cgttccaacc acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc
    10681 tcgtctactc caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt
    10741 ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact
    10801 tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag
    10861 gaaaggctat cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca
    10921 cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat
    10981 gtgatatctc cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt
    11041 cctctatata aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct
    11101 ctacaaatct atctctctcg agtctaccat gagcccagaa cgacgcccgg ccgacatccg
    11161 ccgtgccacc gaggcggaca tgccggcggt ctgcaccatc gtcaaccact acatcgagac
    11221 aagcacggtc aacttccgta ccgagccgca ggaaccgcag gagtggacgg acgacctcgt
    11281 ccgtctgcgg gagcgctatc cctggctcgt cgccgaggtg gacggcgagg tcgccggcat
    11341 cgcctacgcg ggcccctgga aggcacgcaa cgcctacgac tggacggccg agtcgaccgt
    11401 gtacgtctcc ccccgccacc agcggacggg actgggctcc acgctctaca cccacctgct
    11461 gaagtccctg gaggcacagg gcttcaagag cgtggtcgct gtcatcgggc tgcccaacga
    11521 cccgagcgtg cgcatgcacg aggcgctcgg atatgccccc cgcggcatgc tgcgggcggc
    11581 cggcttcaag cacgggaact ggcatgacgt gggtttctgg cagctggact tcagcctgcc
    11641 ggtaccgccc cgtccggtcc tgcccgtcac cgagatttga ctcgagtttc tccataataa
    11701 tgtgtgagta gttcccagat aagggaatta gggttcctat agggtttcgc tcatgtgttg
    11761 agcatataag aaacccttag tatgtatttg tatttgtaaa atacttctat caataaaatt
    11821 tctaattcct aaaaccaaaa tccagtacta aaatccagat cccccgaatt aattcggcgt
    11881 taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt
    11941 ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca
    12001 caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg
    12061 ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc
    12121 tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca
    12181 gagcgttgct gcctgtgatc accgcggttt caaaatcggc tccgtcgata ctatgttata
    12241 cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc
    12301 aaggaacagt gaattggagt tcgtcttgtt ataattagct tcttggggta tctttaaata
    12361 ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga gaatatcacc ggaattgaaa
    12421 aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta
    12481 tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa tgacggacag ccggtataaa
    12541 gggaccacct atgatgtgga acgggaaaag gacatgatgc tatggctgga aggaaagctg
    12601 cctgttccaa aggtcctgca ctttgaacgg catgatggct ggagcaatct gctcatgagt
    12661 gaggccgatg gcgtcctttg ctcggaagag tatgaagatg aacaaagccc tgaaaagatt
    12721 atcgagctgt atgcggagtg catcaggctc tttcactcca tcgacatatc ggattgtccc
    12781 tatacgaata gcttagacag ccgcttagcc gaattggatt acttactgaa taacgatctg
    12841 gccgatgtgg attgcgaaaa ctgggaagaa gacactccat ttaaagatcc gcgcgagctg
    12901 tatgattttt taaagacgga aaagcccgaa gaggaacttg tcttttccca cggcgacctg
    12961 ggagacagca acatctttgt gaaagatggc aaagtaagtg gctttattga tcttgggaga
    13021 agcggcaggg cggacaagtg gtatgacatt gccttctgcg tccggtcgat cagggaggat
    13081 atcggggaag aacagtatgt cgagctattt tttgacttac tggggatcaa gcctgattgg
    13141 gagaaaataa aatattatat tttactggat gaattgtttt agtacctaga atgcatgacc
    13201 aaaatccctt aacgctgatg aatcccctaa tgatttttat caaaatcatt aagttaaggt
    13261 agatacacat cttgtcatat gatcaaatgg tttcgccaaa aatcaataat cagacaacaa
    13321 aatgtgcgaa ctcgatattt tacacgactc tctttaccaa ttctgccccg aattacactt
    13381 aaaacgactc aacagcttaa cgttggcttg ccacgcctta cttgactgta aaactctcac
    13441 tcttaccgaa cttggccgta acctgccaac caaagcgaga acaaaacata acatcaaacg
    13501 aatcgaccga ttgttaggta atcgtcacct ccacaaagag cgactcgctg tataccgttg
    13561 gcatgctagc tttatctgtt cgggcaatac gatgcccatt gtacttgttg actggtctga
    13621 tatccgtgag caaaaacggc ttatggtatt gcgagcttca gtcgcactac acggtcgttc
    13681 tgttactctt tatgagaaag cgttcccgct ttcagagcaa tgttcaaaga aagctcatga
    13741 ccaatttcta gccgaccttg cgagcattct accgagtaac accacaccgc tcattgtcag
    13801 tgatgctggc tttaaagtgc catggtataa atccgttgag aagctgggtt ggtactggtt
    13861 aagtcgagta agaggaaaag tacaatatgc agacctagga gcggaaaact ggaaacctat
    13921 cagcaactta catgatatgt catctagtca ctcaaagact ttaggctata agaggctgac
    13981 taaaagcaat ccaatctcat gccaaattct attgtataaa tctcgctcta aaggccgaaa
    14041 aaatcagcgc tcgacacgga ctcattgtca ccacccgtca cctaaaatct actcagcgtc
    14101 ggcaaaggag ccatggattc tagcaactaa cttacctgtt gaaattcgaa cacccaaaca
    14161 acttgttaat atctattcga agcgaatgca gattgaagaa accttccgag acttgaaaag
    14221 tcctgcctac ggactaggcc tacgccatag ccgaacgagc agctcagagc gttttgatat
    14281 catgctgcta atcgccctga tgcttcaact aacatgttgg cttgcgggcg ttcatgctca
    14341 gaaacaaggt tgggacaagc acttccaggc taacacagtc agaaatcgaa acgtactctc
    14401 aacagttcgc ttaggcatgg aagttttgcg gcattctggc tacacaataa caagggaaga
    14461 cttactcgtg gctgcaaccc tactagctca aaatttattc acacatggtt acgctttggg
    14521 gaaattatga ggggatctct cagcccttaa cgtgagtttt cgttccactg agcgtcagac
    14581 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
    14641 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
    14701 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta
    14761 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
    14821 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
    14881 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
    14941 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
    15001 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
    15061 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
    15121 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
    15181 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
    15241 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
    15301 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
    15361 agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
    15421 tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag
    15481 tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac
    15541 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
    15601 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc
    15661 agggtgcctt gatgtgggcg ccttatttgc cgactacctt ggtgatctcg cctttcacgt
    15721 agtggacaaa ttcttccaac tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa
    15781 gataagcctg tctagcttca agtatgacgg gctgatactg ggccggcagg cgctccattg
    15841 cccagtcggc agcgacatcc ttcggcgcga ttttgccggt tactgcgctg taccaaatgc
    15901 gggacaacgt aagcactaca tttcgctcat cgccagccca gtcgggcggc gagttccata
    15961 gcgttaaggt ttcatttagc gcctcaaata gatcctgttc aggaaccgga tcaaagagtt
    16021 cctccgccgc tggacctacc aaggcaacgc tatgttctct tgcttttgtc agcaagatag
    16081 ccagatcaat gtcgatcgtg gctggctcga agatacctgc aagaatgtca ttgcgctgcc
    16141 attctccaaa ttgcagttcg cgcttagctg gataacgcca cggaatgatg tcgtcgtgca
    16201 caacaatggt gacttctaca gcgcggagaa tctcgctctc tccaggggaa gccgaagttt
    16261 ccaaaaggtc gttgatcaaa gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa
    16321 ccagcaaatc aatatcactg tgtggcttca ggccgccatc cactgcggag ccgtacaaat
    16381 gtacggccag caacgtcggt tcgagatggc gctcgatgac gccaactacc tctgatagtt
    16441 gagtcgatac ttcggcgatc accgcttccc tcat
//
