LOCUS       sequence-205505-        9621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9621)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-311
FEATURES             Location/Qualifiers
     source          1..9621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     563..582
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(602..679)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     885..904
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     988..1007
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        988..1006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1007..1031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1085..1102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4650..4739
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4752..4841
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5543..5561)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5557..5604
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5641..6096
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5728..5747)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5938..5959
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6188..7003)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6188..7003)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6911..6930
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6995..7014
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7125..7713
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7614..7633
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7867..7884
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7899..8038
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7964..7983)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8124..8146
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8140..8331)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9140..9621,1..601))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
       61 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtcc tcatgggaga
      121 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
      181 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
      241 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
      301 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
      361 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
      421 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
      481 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
      541 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
      601 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
      661 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      721 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      781 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      841 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     1021 ataacaattc ccctctagaa ataattttgc cttaacttta agaaggagat ataccatggg
     1081 cagccaccac caccaccacc acagcggaat gcagatcttt gttaagactc tcaccggaaa
     1141 gacaatcacc ctagaggtgg aaagctccga caccatcgac aacgttaagg ccaagatcca
     1201 ggataaggag ggcattcctc cggatcagca gaggcttatt ttcgccggca agcagctaga
     1261 ggatggccgt acgttggctg attacaatat ccagaaggaa tccaccctcc acttggtcct
     1321 caggctccgt ggtggttaag cttgcaatta actttaagaa ggagatatac aatgcttcac
     1381 aagcgagcta gtgaagctaa cgataagaac gataatacca tcatcggttc cgatttagca
     1441 tcatctaaga aacgccgtat cgatttcact gaatcttcgt ctgataagtc atcttcgatt
     1501 ttagcttctg gtagtagcag gggttttcac ggcgatagcg tcgttcagca aatcgacatg
     1561 gcttttggta attcgaaccg tcaggagatt gatgaagatc tgcacagtcg ccagctcgcc
     1621 gtctatggtc gtgagactat gaggcgtctc tttgcttcca atgttctcat ctcggggatg
     1681 cacggtcttg gtgccgagat tgccaagaat cttatacttg ctggtgtgaa gtctgtgacc
     1741 ttgcatgatg aaagagttgt agagctatgg gacttatcaa gcaactttgt tttctcggag
     1801 gatgatgttg gcaagaatag agcagatgct tctgttcaga agttacagga tcttaacaat
     1861 gctgtggttg tctctagctt gaccaaaagc ttaaacaaag aggatctttc tggcttccag
     1921 gttgttgtat tctctgacat aagcatggaa agagcaatcg aatttgatga ctattgtcac
     1981 agccatcagc ctcctatcgc ttttgtcaag gctgatgtca ggggtctttt cggctctgtc
     2041 ttttgtgatt ttgggcctga atttgcggtt cttgatgttg atggagagga accccataca
     2101 ggcatcattg cgtctatctc taatgagaac caggcattta tttcctgtgt cgacgatgag
     2161 aggcttgagt ttgaagatgg tgacctagtt gttttctcag aagttgaggg tatgacagaa
     2221 ttgaatgatg ggaaaccaag gaagataaaa agtacacggc catattcatt cacccttgat
     2281 gaggacacta ctaactatgg aacatatgtg aagggtggta ttgtcactca agtgaaacag
     2341 ccaaagttac tgaatttcaa gccattgagg gaagcgctta aggatccagg ggattttcta
     2401 tttagtgatt tctctaagtt tgatcggcct ccactccttc acttagcatt ccaggcactt
     2461 gatcatttta aagctgaagc tggcagattt cctgttgctg gctcagaaga ggacgctcag
     2521 aagcttatat cgattgccac agccatcaat accgggcagg gtgatttaaa ggtggagaat
     2581 gttgatcaga agcttctaag acacttttcc tttggagcca aggctgtcct gaatcctatg
     2641 gctgcaatgt ttggcggtat tgttggacag gaggttgtta aagcttgctc tggaaaattt
     2701 catcctctct tccagttttt ctactttgat tcagtggagt cactcccctc tgaacctgtg
     2761 gactcgagtg actttgcacc aagaaacagc cggtatgatg cccaaatatc tgtatttggg
     2821 gccaagttcc agaagaaact tgaagatgct aaagttttca cagtagggtc tggtgctctt
     2881 ggctgtgagt tcttgaaaaa tttggctctg atgggggttt cttgtggaag ccaagggaag
     2941 ctgacagtga cagatgatga tatcattgag aagagtaacc tcagtcgtca atttctgttc
     3001 cgtgactgga acattggaca ggctaaatcc acagttgctg cttcggctgc tgctgttata
     3061 aatcctcgct tcaacattga ggccctgcaa aaccgtgttg gcgcggagac tgagaatgta
     3121 tttgacgatg ccttctggga gaacttaact gttgtcgtca atgcactgga taatgttaat
     3181 gcgaggctct atgttgattc gaggtgcttg tatttccaga agcctctcct tgagtctgga
     3241 actcttggta caaagtgcaa cacacagtcg gttatcccac atctaactga aaattacggt
     3301 gcctcaaggg acccaccaga gaaacaggcc cccatgtgta cagtgcattc cttcccgcat
     3361 aacattgatc actgtttaac ttgggctcgc tctgagtttg agggtctgct ggagaaaact
     3421 cccgctgaag tgaatgcata tctttctagc ccagttgagt acactaactc aatgatgagt
     3481 gctggtgatg ctcaggcgag ggacacattg gagaggattg ttgagtgcct tgaaaaggag
     3541 aagtgtgaaa ccttccaaga ctgcttaacc tgggcacgac tgaggtttga ggattacttt
     3601 gtgaaccgtg tcaagcaatt gatatacaca tttcctgaag atgctgcgac tagtaccgga
     3661 gctccattct ggtctgctcc aaaaagattc cctcgtccac tccagtactc gtcgtccgac
     3721 ccaagtctcc ttaactttat cacagcgact gctattttac gagcagagac atttgggatc
     3781 ccgatacctg agtggactaa gaacccaaag gaagcagcag aagctgttga cagagtgata
     3841 gtcccagact ttgagccaag gcaagatgca aagattgtga ctgatgagaa agctaccact
     3901 ttaaccactg cttcggtgga cgacgctgca gtcatcgacg atctcattgc taagatcgac
     3961 cagtgtaggc ataacttgtc gccagacttc aggatgaaac ctattcagtt cgaaaaggat
     4021 gatgatacaa actatcacat ggatgtgata gcaggactag ccaacatgag ggcgagaaat
     4081 tacagcatcc ctgaagtgga caagctgaaa gcaaagttca tagcagggag aatcatccca
     4141 gccattgcga cttcaacagc catggctact gggctggtct gcctcgagct gtacaaggtc
     4201 cttgatggag gacataaagt ggaagcttac agaaacacat ttgccaacct ggcacttcca
     4261 ctattctcca tggctgaacc acttccacca aaggtggtga agcaccgtga catggcttgg
     4321 acagtttggg acagatgggt tctcaaggga aacccaactc ttcgtgaggt gttgcagtgg
     4381 ctggaggaca aagggcttag cgcatacagc atctcttgtg gaagctgtct actgttcaac
     4441 agtatgttca caaggcacaa ggagaggatg gacaagaagg ttgtggatct cgctcgggat
     4501 gttgcgaaag tcgagttacc gccttatcgc aaccatcttg atgtagtggt ggcttgtgag
     4561 gatgaagatg acaatgacgt cgatattcct ctcgtctcta tctacttcag gtgagcttac
     4621 tattaacttt aagaaggaga tataccatgt acccatacga tgttcctgac tatgcgggct
     4681 atccctatga cgtcccggac tatgcaggat tgtatccata tgacgttcca gattacgcca
     4741 ctagagctgc ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
     4801 actatgcagg attgtatcca tatgacgttc cagattacgc aatggccgcc gtaaatggat
     4861 atcagggaaa tactccggca gatcctccgg cgtctaatgg atcaaaacaa cctgcggctc
     4921 cgaccaagac tgttgacagc caatctgttc tcaaaagact gcaatctgaa ttgatgggct
     4981 tgatgatggg cggtggtcca ggaatatcgg ctttccctga ggaggacaac atattctgct
     5041 ggaaagggac aataacagga agcaaagata ctgtgtttga aggaactgag tacagactct
     5101 cactctcttt ctccaatgac tatcctttca agcctccaaa ggttaagttt gagacatgtt
     5161 gcttccaccc caatgttgat gtctatggca atatctgctt agacattctt caggataagt
     5221 ggtcatctgc ttacgatgtg aggacgatac tactatcgat tcagagcctc ctgggagaac
     5281 cgaacatcag ttcaccattg aacactcaag cagctcagct ttggagtaac caagaagagt
     5341 acaggaagat ggttgagaag ctttacaagc ctccaagtgc atgagctttt acgcttttaa
     5401 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5461 catccgctta cagacaagct gtgaccggga tgagatccgg ctgctaacaa agcccgaaag
     5521 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     5581 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
     5641 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5701 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     5761 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     5821 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     5881 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     5941 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6001 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6061 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa
     6181 ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     6241 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     6301 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     6361 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     6421 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     6481 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     6541 gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     6601 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     6661 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     6721 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     6781 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     6841 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     6901 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     6961 atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     7021 tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg
     7081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7261 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7801 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7861 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     7921 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     7981 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     8041 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8101 ttacagacaa gctgtgaccg actccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8161 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     8221 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     8281 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     8341 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     8401 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     8461 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     8521 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     8581 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     8641 caagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     8701 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     8761 ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat
     8821 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     8881 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     8941 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     9001 gaacacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     9061 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     9121 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9181 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
     9241 tttttctttt caccagtgac acgggcaaca gctgattgcc cttcaccgcc tggccctgag
     9301 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
     9361 tggttaacgg cgggatataa catgagctgt cctcggtatc gtcgtatccc actaccgaga
     9421 tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     9481 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     9541 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     9601 tgcgagtgag atatttatgc c
//
