LOCUS       sequence-205509-        9552 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9552)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9552)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-312
FEATURES             Location/Qualifiers
     source          1..9552
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     932..951
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(971..1048)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1254..1273
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1357..1376
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1357..1375
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1376..1400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1454..1471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5019..5108
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5121..5210
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5843..5861)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5857..5904
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5941..6396
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6028..6047)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6238..6259
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6488..7303)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6488..7303)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7211..7230
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7295..7314
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7425..8013
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7914..7933
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8167..8184
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8199..8338
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8264..8283)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8424..8446
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8440..8631)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9440..9552,1..970))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ccagtgacac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
       61 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
      121 ggatataaca tgagctgtcc tcggtatcgt cgtatcccac taccgagata tccgcaccaa
      181 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
      241 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
      301 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
      361 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
      421 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtcct
      481 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
      541 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
      601 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
      661 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
      721 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
      781 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
      841 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
      901 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
      961 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     1021 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     1081 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     1141 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     1201 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     1261 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     1321 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     1381 tgtgagcgga taacaattcc cctctagaaa taattttgcc ttaactttaa gaaggagata
     1441 taccatgggc agccaccacc accaccacca cagcggaatg cagatctttg ttaagactct
     1501 caccggaaag acaatcaccc tagaggtgga aagctccgac accatcgaca acgttaaggc
     1561 caagatccag gataaggagg gcattcctcc ggatcagcag aggcttattt tcgccggcaa
     1621 gcagctagag gatggccgta cgttggctga ttacaatatc cagaaggaat ccaccctcca
     1681 cttggtcctc aggctccgtg gtggttaagc ttgcaattaa ctttaagaag gagatataca
     1741 atgcttcaca agcgagctag tgaagctaac gataagaacg ataataccat catcggttcc
     1801 gatttagcat catctaagaa acgccgtatc gatttcactg aatcttcgtc tgataagtca
     1861 tcttcgattt tagcttctgg tagtagcagg ggttttcacg gcgatagcgt cgttcagcaa
     1921 atcgacatgg cttttggtaa ttcgaaccgt caggagattg atgaagatct gcacagtcgc
     1981 cagctcgccg tctatggtcg tgagactatg aggcgtctct ttgcttccaa tgttctcatc
     2041 tcggggatgc acggtcttgg tgccgagatt gccaagaatc ttatacttgc tggtgtgaag
     2101 tctgtgacct tgcatgatga aagagttgta gagctatggg acttatcaag caactttgtt
     2161 ttctcggagg atgatgttgg caagaataga gcagatgctt ctgttcagaa gttacaggat
     2221 cttaacaatg ctgtggttgt ctctagcttg accaaaagct taaacaaaga ggatctttct
     2281 ggcttccagg ttgttgtatt ctctgacata agcatggaaa gagcaatcga atttgatgac
     2341 tattgtcaca gccatcagcc tcctatcgct tttgtcaagg ctgatgtcag gggtcttttc
     2401 ggctctgtct tttgtgattt tgggcctgaa tttgcggttc ttgatgttga tggagaggaa
     2461 ccccatacag gcatcattgc gtctatctct aatgagaacc aggcatttat ttcctgtgtc
     2521 gacgatgaga ggcttgagtt tgaagatggt gacctagttg ttttctcaga agttgagggt
     2581 atgacagaat tgaatgatgg gaaaccaagg aagataaaaa gtacacggcc atattcattc
     2641 acccttgatg aggacactac taactatgga acatatgtga agggtggtat tgtcactcaa
     2701 gtgaaacagc caaagttact gaatttcaag ccattgaggg aagcgcttaa ggatccaggg
     2761 gattttctat ttagtgattt ctctaagttt gatcggcctc cactccttca cttagcattc
     2821 caggcacttg atcattttaa agctgaagct ggcagatttc ctgttgctgg ctcagaagag
     2881 gacgctcaga agcttatatc gattgccaca gccatcaata ccgggcaggg tgatttaaag
     2941 gtggagaatg ttgatcagaa gcttctaaga cacttttcct ttggagccaa ggctgtcctg
     3001 aatcctatgg ctgcaatgtt tggcggtatt gttggacagg aggttgttaa agcttgctct
     3061 ggaaaatttc atcctctctt ccagtttttc tactttgatt cagtggagtc actcccctct
     3121 gaacctgtgg actcgagtga ctttgcacca agaaacagcc ggtatgatgc ccaaatatct
     3181 gtatttgggg ccaagttcca gaagaaactt gaagatgcta aagttttcac agtagggtct
     3241 ggtgctcttg gctgtgagtt cttgaaaaat ttggctctga tgggggtttc ttgtggaagc
     3301 caagggaagc tgacagtgac agatgatgat atcattgaga agagtaacct cagtcgtcaa
     3361 tttctgttcc gtgactggaa cattggacag gctaaatcca cagttgctgc ttcggctgct
     3421 gctgttataa atcctcgctt caacattgag gccctgcaaa accgtgttgg cgcggagact
     3481 gagaatgtat ttgacgatgc cttctgggag aacttaactg ttgtcgtcaa tgcactggat
     3541 aatgttaatg cgaggctcta tgttgattcg aggtgcttgt atttccagaa gcctctcctt
     3601 gagtctggaa ctcttggtac aaagtgcaac acacagtcgg ttatcccaca tctaactgaa
     3661 aattacggtg cctcaaggga cccaccagag aaacaggccc ccatgtgtac agtgcattcc
     3721 ttcccgcata acattgatca ctgtttaact tgggctcgct ctgagtttga gggtctgctg
     3781 gagaaaactc ccgctgaagt gaatgcatat ctttctagcc cagttgagta cactaactca
     3841 atgatgagtg ctggtgatgc tcaggcgagg gacacattgg agaggattgt tgagtgcctt
     3901 gaaaaggaga agtgtgaaac cttccaagac tgcttaacct gggcacgact gaggtttgag
     3961 gattactttg tgaaccgtgt caagcaattg atatacacat ttcctgaaga tgctgcgact
     4021 agtaccggag ctccattctg gtctgctcca aaaagattcc ctcgtccact ccagtactcg
     4081 tcgtccgacc caagtctcct taactttatc acagcgactg ctattttacg agcagagaca
     4141 tttgggatcc cgatacctga gtggactaag aacccaaagg aagcagcaga agctgttgac
     4201 agagtgatag tcccagactt tgagccaagg caagatgcaa agattgtgac tgatgagaaa
     4261 gctaccactt taaccactgc ttcggtggac gacgctgcag tcatcgacga tctcattgct
     4321 aagatcgacc agtgtaggca taacttgtcg ccagacttca ggatgaaacc tattcagttc
     4381 gaaaaggatg atgatacaaa ctatcacatg gatgtgatag caggactagc caacatgagg
     4441 gcgagaaatt acagcatccc tgaagtggac aagctgaaag caaagttcat agcagggaga
     4501 atcatcccag ccattgcgac ttcaacagcc atggctactg ggctggtctg cctcgagctg
     4561 tacaaggtcc ttgatggagg acataaagtg gaagcttaca gaaacacatt tgccaacctg
     4621 gcacttccac tattctccat ggctgaacca cttccaccaa aggtggtgaa gcaccgtgac
     4681 atggcttgga cagtttggga cagatgggtt ctcaagggaa acccaactct tcgtgaggtg
     4741 ttgcagtggc tggaggacaa agggcttagc gcatacagca tctcttgtgg aagctgtcta
     4801 ctgttcaaca gtatgttcac aaggcacaag gagaggatgg acaagaaggt tgtggatctc
     4861 gctcgggatg ttgcgaaagt cgagttaccg ccttatcgca accatcttga tgtagtggtg
     4921 gcttgtgagg atgaagatga caatgacgtc gatattcctc tcgtctctat ctacttcagg
     4981 tgagcttact attaacttta agaaggagat ataccatgta cccatacgat gttcctgact
     5041 atgcgggcta tccctatgac gtcccggact atgcaggatt gtatccatat gacgttccag
     5101 attacgccac tagagctgct tacccatacg atgttcctga ctatgcgggc tatccctatg
     5161 acgtcccgga ctatgcagga ttgtatccat atgacgttcc agattacgca atgcaggcat
     5221 caagagcgcg actgtttaaa gaatacaaag aggtacagcg agagaaagta gcagatcctg
     5281 atattcaatt gatctgtgac gataccaaca tattcaaatg gaccgctctt atcaaaggac
     5341 cttcggagac tccttacgaa ggcggtgttt ttcagcttgc tttttctgtt cctgaacctt
     5401 atcctttgca acctcctcaa gttcgattct tgaccaagat attccatcct aatgttcatt
     5461 tcaagacagg agaaatatgt ctcgacattt tgaagaatgc ttggagtcct gcttggacgc
     5521 ttcagtctgt gtgtagagct atcatagcat tgatggctca tcctgagccg gacagtcctc
     5581 ttaactgcga ctcagggaat cttctaagat ctggtgatgt gagagggttc aattcaatgg
     5641 cacaaatgta tacacgcctc gccgctatgc ctaagaaagg atgagctttt acgcttttaa
     5701 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5761 catccgctta cagacaagct gtgaccggga tgagatccgg ctgctaacaa agcccgaaag
     5821 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     5881 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
     5941 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6001 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6061 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6121 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6181 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6241 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6301 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6361 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6421 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa
     6481 ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     6541 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     6601 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     6661 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     6721 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     6781 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     6841 gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     6901 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     6961 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     7021 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     7081 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     7141 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     7201 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     7261 atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     7321 tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg
     7381 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7441 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7501 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     7561 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     7621 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     7681 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7741 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7801 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7861 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7921 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7981 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8041 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8101 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8161 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     8221 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     8281 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     8341 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8401 ttacagacaa gctgtgaccg actccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8461 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     8521 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     8581 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     8641 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     8701 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     8761 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     8821 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     8881 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     8941 caagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     9001 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     9061 ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat
     9121 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     9181 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     9241 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     9301 gaacacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     9361 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     9421 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9481 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
     9541 tttttctttt ca
//
