LOCUS       sequence-205517-        9834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9834)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-313
FEATURES             Location/Qualifiers
     source          1..9834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     965..984
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1004..1081)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1287..1306
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1390..1409
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1390..1408
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1409..1433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1487..1504
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5052..5141
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5154..5243
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6158..6176)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6172..6219
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6256..6711
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6343..6362)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6553..6574
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6803..7618)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6803..7618)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7526..7545
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7610..7629
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7740..8328
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8229..8248
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8482..8499
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8514..8653
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8579..8598)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8739..8761
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8755..8946)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9755..9834,1..1003))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gcgtattggg cgccagggtg gtttttcttt tcaccagtga cacgggcaac agctgattgc
       61 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca
      121 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcctcggtat
      181 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc
      241 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct
      301 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt
      361 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac
      421 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga
      481 ccagatgctc cacgcccagt cgcgtaccgt cctcatggga gaaaataata ctgttgatgg
      541 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag
      601 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga
      661 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca
      721 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg
      781 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt
      841 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc
      901 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga
      961 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt
     1021 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt
     1081 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     1141 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac
     1201 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     1261 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     1321 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc
     1381 ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag
     1441 aaataatttt gccttaactt taagaaggag atataccatg ggcagccacc accaccacca
     1501 ccacagcgga atgcagatct ttgttaagac tctcaccgga aagacaatca ccctagaggt
     1561 ggaaagctcc gacaccatcg acaacgttaa ggccaagatc caggataagg agggcattcc
     1621 tccggatcag cagaggctta ttttcgccgg caagcagcta gaggatggcc gtacgttggc
     1681 tgattacaat atccagaagg aatccaccct ccacttggtc ctcaggctcc gtggtggtta
     1741 agcttgcaat taactttaag aaggagatat acaatgcttc acaagcgagc tagtgaagct
     1801 aacgataaga acgataatac catcatcggt tccgatttag catcatctaa gaaacgccgt
     1861 atcgatttca ctgaatcttc gtctgataag tcatcttcga ttttagcttc tggtagtagc
     1921 aggggttttc acggcgatag cgtcgttcag caaatcgaca tggcttttgg taattcgaac
     1981 cgtcaggaga ttgatgaaga tctgcacagt cgccagctcg ccgtctatgg tcgtgagact
     2041 atgaggcgtc tctttgcttc caatgttctc atctcgggga tgcacggtct tggtgccgag
     2101 attgccaaga atcttatact tgctggtgtg aagtctgtga ccttgcatga tgaaagagtt
     2161 gtagagctat gggacttatc aagcaacttt gttttctcgg aggatgatgt tggcaagaat
     2221 agagcagatg cttctgttca gaagttacag gatcttaaca atgctgtggt tgtctctagc
     2281 ttgaccaaaa gcttaaacaa agaggatctt tctggcttcc aggttgttgt attctctgac
     2341 ataagcatgg aaagagcaat cgaatttgat gactattgtc acagccatca gcctcctatc
     2401 gcttttgtca aggctgatgt caggggtctt ttcggctctg tcttttgtga ttttgggcct
     2461 gaatttgcgg ttcttgatgt tgatggagag gaaccccata caggcatcat tgcgtctatc
     2521 tctaatgaga accaggcatt tatttcctgt gtcgacgatg agaggcttga gtttgaagat
     2581 ggtgacctag ttgttttctc agaagttgag ggtatgacag aattgaatga tgggaaacca
     2641 aggaagataa aaagtacacg gccatattca ttcacccttg atgaggacac tactaactat
     2701 ggaacatatg tgaagggtgg tattgtcact caagtgaaac agccaaagtt actgaatttc
     2761 aagccattga gggaagcgct taaggatcca ggggattttc tatttagtga tttctctaag
     2821 tttgatcggc ctccactcct tcacttagca ttccaggcac ttgatcattt taaagctgaa
     2881 gctggcagat ttcctgttgc tggctcagaa gaggacgctc agaagcttat atcgattgcc
     2941 acagccatca ataccgggca gggtgattta aaggtggaga atgttgatca gaagcttcta
     3001 agacactttt cctttggagc caaggctgtc ctgaatccta tggctgcaat gtttggcggt
     3061 attgttggac aggaggttgt taaagcttgc tctggaaaat ttcatcctct cttccagttt
     3121 ttctactttg attcagtgga gtcactcccc tctgaacctg tggactcgag tgactttgca
     3181 ccaagaaaca gccggtatga tgcccaaata tctgtatttg gggccaagtt ccagaagaaa
     3241 cttgaagatg ctaaagtttt cacagtaggg tctggtgctc ttggctgtga gttcttgaaa
     3301 aatttggctc tgatgggggt ttcttgtgga agccaaggga agctgacagt gacagatgat
     3361 gatatcattg agaagagtaa cctcagtcgt caatttctgt tccgtgactg gaacattgga
     3421 caggctaaat ccacagttgc tgcttcggct gctgctgtta taaatcctcg cttcaacatt
     3481 gaggccctgc aaaaccgtgt tggcgcggag actgagaatg tatttgacga tgccttctgg
     3541 gagaacttaa ctgttgtcgt caatgcactg gataatgtta atgcgaggct ctatgttgat
     3601 tcgaggtgct tgtatttcca gaagcctctc cttgagtctg gaactcttgg tacaaagtgc
     3661 aacacacagt cggttatccc acatctaact gaaaattacg gtgcctcaag ggacccacca
     3721 gagaaacagg cccccatgtg tacagtgcat tccttcccgc ataacattga tcactgttta
     3781 acttgggctc gctctgagtt tgagggtctg ctggagaaaa ctcccgctga agtgaatgca
     3841 tatctttcta gcccagttga gtacactaac tcaatgatga gtgctggtga tgctcaggcg
     3901 agggacacat tggagaggat tgttgagtgc cttgaaaagg agaagtgtga aaccttccaa
     3961 gactgcttaa cctgggcacg actgaggttt gaggattact ttgtgaaccg tgtcaagcaa
     4021 ttgatataca catttcctga agatgctgcg actagtaccg gagctccatt ctggtctgct
     4081 ccaaaaagat tccctcgtcc actccagtac tcgtcgtccg acccaagtct ccttaacttt
     4141 atcacagcga ctgctatttt acgagcagag acatttggga tcccgatacc tgagtggact
     4201 aagaacccaa aggaagcagc agaagctgtt gacagagtga tagtcccaga ctttgagcca
     4261 aggcaagatg caaagattgt gactgatgag aaagctacca ctttaaccac tgcttcggtg
     4321 gacgacgctg cagtcatcga cgatctcatt gctaagatcg accagtgtag gcataacttg
     4381 tcgccagact tcaggatgaa acctattcag ttcgaaaagg atgatgatac aaactatcac
     4441 atggatgtga tagcaggact agccaacatg agggcgagaa attacagcat ccctgaagtg
     4501 gacaagctga aagcaaagtt catagcaggg agaatcatcc cagccattgc gacttcaaca
     4561 gccatggcta ctgggctggt ctgcctcgag ctgtacaagg tccttgatgg aggacataaa
     4621 gtggaagctt acagaaacac atttgccaac ctggcacttc cactattctc catggctgaa
     4681 ccacttccac caaaggtggt gaagcaccgt gacatggctt ggacagtttg ggacagatgg
     4741 gttctcaagg gaaacccaac tcttcgtgag gtgttgcagt ggctggagga caaagggctt
     4801 agcgcataca gcatctcttg tggaagctgt ctactgttca acagtatgtt cacaaggcac
     4861 aaggagagga tggacaagaa ggttgtggat ctcgctcggg atgttgcgaa agtcgagtta
     4921 ccgccttatc gcaaccatct tgatgtagtg gtggcttgtg aggatgaaga tgacaatgac
     4981 gtcgatattc ctctcgtctc tatctacttc aggtgagctt actattaact ttaagaagga
     5041 gatataccat gtacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
     5101 actatgcagg attgtatcca tatgacgttc cagattacgc cactagagct gcttacccat
     5161 acgatgttcc tgactatgcg ggctatccct atgacgtccc ggactatgca ggattgtatc
     5221 catatgacgt tccagattac gcaatggcta gtaatgagaa tctaccaccc aatgttatca
     5281 agcagcttgc caaggagctg aagagtcttg acgaatcccc tccagatggc atcaaggttg
     5341 tggtaaatga cgaggatttc tcacaaatat gtgctgatat tgaaggacca gttggtactc
     5401 cttatgagaa cggacttttc cgtatgaagt tggccttgtc acatgatttt ccacattcgc
     5461 ctcccaaagg ctacttcatg acaaagattt tccatcctaa tgttgcttct aatggagaga
     5521 tatgtgttaa cacgcttaaa aaagattgga accctagtct tggattaaga catgttctct
     5581 ctgttgtaag gtgcttatta atcgagccat ttccagaatc tgcattaaat gaacaagctg
     5641 gcaagatgct acttgaaaac tatgacgagt atgcgaggca tgctaggctt tacacaggta
     5701 tccatgctaa accaaaaccc aagttcaaga caggagcaat ctcagagtca acaaccgctc
     5761 taaatgttgg ccagacgaac aacgagacgc ctggtgctgc tactgcaata ccctcttcaa
     5821 tgacagacat aaagagagta acaacgagtg cccaagacca acaacacgta gctaatgtcg
     5881 ttgtagcagc ctctgcaagt gtggtcacta cgacgcaaaa aagggaagca ggtttggcca
     5941 aggttcaggc agataagaag aaggtagacg ctagaaagaa aagcttgaag agactatgag
     6001 cttttacgct tttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     6061 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgggatgaga tccggctgct
     6121 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     6181 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     6241 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6301 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6361 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6421 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6481 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6541 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6601 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6661 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6721 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6781 gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt
     6841 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     6901 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     6961 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     7021 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     7081 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     7141 aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag
     7201 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     7261 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     7321 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     7381 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     7441 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     7501 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     7561 ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa
     7621 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct
     7681 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7741 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7801 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7861 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7921 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7981 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     8041 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     8101 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     8161 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     8221 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     8281 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8341 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8401 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8461 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     8521 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta
     8581 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     8641 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     8701 gctcccggca tccgcttaca gacaagctgt gaccgactcc gggagctgca tgtgtcagag
     8761 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     8821 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     8881 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     8941 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     9001 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     9061 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     9121 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     9181 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     9241 cgaaacacgg aaacccaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     9301 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     9361 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     9421 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     9481 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     9541 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     9601 gagttgcatg ataaagaaca cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     9661 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     9721 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gttt
//
