LOCUS       sequence-205521-       12387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 12387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-314
FEATURES             Location/Qualifiers
     source          1..12387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     126..145
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     229..248
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        229..247
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    248..272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             326..343
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3891..3980
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3993..4082
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(7550..7568)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      7564..7611
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      7648..8103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7735..7754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7945..7966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8195..9010)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8195..9010)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8918..8937
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9002..9021
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9132..9720
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9621..9640
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9874..9891
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9906..10045
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9971..9990)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10131..10153
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10147..10338)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(11147..12229)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     12191..12210
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(12230..12307)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
       61 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
      121 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
      181 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
      241 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg ccttaacttt
      301 aagaaggaga tataccatgg gcagccacca ccaccaccac cacagcggaa tgcagatctt
      361 tgttaagact ctcaccggaa agacaatcac cctagaggtg gaaagctccg acaccatcga
      421 caacgttaag gccaagatcc aggataagga gggcattcct ccggatcagc agaggcttat
      481 tttcgccggc aagcagctag aggatggccg tacgttggct gattacaata tccagaagga
      541 atccaccctc cacttggtcc tcaggctccg tggtggttaa gcttgcaatt aactttaaga
      601 aggagatata caatgcttca caagcgagct agtgaagcta acgataagaa cgataatacc
      661 atcatcggtt ccgatttagc atcatctaag aaacgccgta tcgatttcac tgaatcttcg
      721 tctgataagt catcttcgat tttagcttct ggtagtagca ggggttttca cggcgatagc
      781 gtcgttcagc aaatcgacat ggcttttggt aattcgaacc gtcaggagat tgatgaagat
      841 ctgcacagtc gccagctcgc cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc
      901 aatgttctca tctcggggat gcacggtctt ggtgccgaga ttgccaagaa tcttatactt
      961 gctggtgtga agtctgtgac cttgcatgat gaaagagttg tagagctatg ggacttatca
     1021 agcaactttg ttttctcgga ggatgatgtt ggcaagaata gagcagatgc ttctgttcag
     1081 aagttacagg atcttaacaa tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa
     1141 gaggatcttt ctggcttcca ggttgttgta ttctctgaca taagcatgga aagagcaatc
     1201 gaatttgatg actattgtca cagccatcag cctcctatcg cttttgtcaa ggctgatgtc
     1261 aggggtcttt tcggctctgt cttttgtgat tttgggcctg aatttgcggt tcttgatgtt
     1321 gatggagagg aaccccatac aggcatcatt gcgtctatct ctaatgagaa ccaggcattt
     1381 atttcctgtg tcgacgatga gaggcttgag tttgaagatg gtgacctagt tgttttctca
     1441 gaagttgagg gtatgacaga attgaatgat gggaaaccaa ggaagataaa aagtacacgg
     1501 ccatattcat tcacccttga tgaggacact actaactatg gaacatatgt gaagggtggt
     1561 attgtcactc aagtgaaaca gccaaagtta ctgaatttca agccattgag ggaagcgctt
     1621 aaggatccag gggattttct atttagtgat ttctctaagt ttgatcggcc tccactcctt
     1681 cacttagcat tccaggcact tgatcatttt aaagctgaag ctggcagatt tcctgttgct
     1741 ggctcagaag aggacgctca gaagcttata tcgattgcca cagccatcaa taccgggcag
     1801 ggtgatttaa aggtggagaa tgttgatcag aagcttctaa gacacttttc ctttggagcc
     1861 aaggctgtcc tgaatcctat ggctgcaatg tttggcggta ttgttggaca ggaggttgtt
     1921 aaagcttgct ctggaaaatt tcatcctctc ttccagtttt tctactttga ttcagtggag
     1981 tcactcccct ctgaacctgt ggactcgagt gactttgcac caagaaacag ccggtatgat
     2041 gcccaaatat ctgtatttgg ggccaagttc cagaagaaac ttgaagatgc taaagttttc
     2101 acagtagggt ctggtgctct tggctgtgag ttcttgaaaa atttggctct gatgggggtt
     2161 tcttgtggaa gccaagggaa gctgacagtg acagatgatg atatcattga gaagagtaac
     2221 ctcagtcgtc aatttctgtt ccgtgactgg aacattggac aggctaaatc cacagttgct
     2281 gcttcggctg ctgctgttat aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt
     2341 ggcgcggaga ctgagaatgt atttgacgat gccttctggg agaacttaac tgttgtcgtc
     2401 aatgcactgg ataatgttaa tgcgaggctc tatgttgatt cgaggtgctt gtatttccag
     2461 aagcctctcc ttgagtctgg aactcttggt acaaagtgca acacacagtc ggttatccca
     2521 catctaactg aaaattacgg tgcctcaagg gacccaccag agaaacaggc ccccatgtgt
     2581 acagtgcatt ccttcccgca taacattgat cactgtttaa cttgggctcg ctctgagttt
     2641 gagggtctgc tggagaaaac tcccgctgaa gtgaatgcat atctttctag cccagttgag
     2701 tacactaact caatgatgag tgctggtgat gctcaggcga gggacacatt ggagaggatt
     2761 gttgagtgcc ttgaaaagga gaagtgtgaa accttccaag actgcttaac ctgggcacga
     2821 ctgaggtttg aggattactt tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa
     2881 gatgctgcga ctagtaccgg agctccattc tggtctgctc caaaaagatt ccctcgtcca
     2941 ctccagtact cgtcgtccga cccaagtctc cttaacttta tcacagcgac tgctatttta
     3001 cgagcagaga catttgggat cccgatacct gagtggacta agaacccaaa ggaagcagca
     3061 gaagctgttg acagagtgat agtcccagac tttgagccaa ggcaagatgc aaagattgtg
     3121 actgatgaga aagctaccac tttaaccact gcttcggtgg acgacgctgc agtcatcgac
     3181 gatctcattg ctaagatcga ccagtgtagg cataacttgt cgccagactt caggatgaaa
     3241 cctattcagt tcgaaaagga tgatgataca aactatcaca tggatgtgat agcaggacta
     3301 gccaacatga gggcgagaaa ttacagcatc cctgaagtgg acaagctgaa agcaaagttc
     3361 atagcaggga gaatcatccc agccattgcg acttcaacag ccatggctac tgggctggtc
     3421 tgcctcgagc tgtacaaggt ccttgatgga ggacataaag tggaagctta cagaaacaca
     3481 tttgccaacc tggcacttcc actattctcc atggctgaac cacttccacc aaaggtggtg
     3541 aagcaccgtg acatggcttg gacagtttgg gacagatggg ttctcaaggg aaacccaact
     3601 cttcgtgagg tgttgcagtg gctggaggac aaagggctta gcgcatacag catctcttgt
     3661 ggaagctgtc tactgttcaa cagtatgttc acaaggcaca aggagaggat ggacaagaag
     3721 gttgtggatc tcgctcggga tgttgcgaaa gtcgagttac cgccttatcg caaccatctt
     3781 gatgtagtgg tggcttgtga ggatgaagat gacaatgacg tcgatattcc tctcgtctct
     3841 atctacttca ggtgagctta ctattaactt taagaaggag atataccatg tacccatacg
     3901 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga ttgtatccat
     3961 atgacgttcc agattacgcc actagagctg cttacccata cgatgttcct gactatgcgg
     4021 gctatcccta tgacgtcccg gactatgcag gattgtatcc atatgacgtt ccagattacg
     4081 caatggagca tgagcaagat gaccctggta catcaacaaa tgtaggggtt gactcgtcag
     4141 ttgatgattc aatggcaagt ctctctatat gtgattcaga gcatcctaat atttatcgac
     4201 aagatattgt caaaaacaaa aagaccggta gtgttggtgt tgtcagcgaa gtagctggtg
     4261 attctgactc tgatagtgat attagtgatg aggaagaaga tgatgatgat gacgaagata
     4321 atgatgatga tgatgaggat gtagaggaag ggaaaaaggc tagtgaagaa aatgttgtta
     4381 atggagatgg cgagaaaaaa gctgatggga attataaatg tggtgcactt gagggagatc
     4441 agattcgtgt gctttggatg gataacactg agcctgttca agatattaat gatgtaactg
     4501 ttatagaccg tggtttctta catggtgatt atgttgcttc tgcttctgag cctactggtc
     4561 aggtgggagt tgttgtggat gttaacatat cagttgattt gttggctcct gatggttcta
     4621 ttcacaagga catctctacc aagaatttga aacgcgtcag ggattttgct gttggtgatt
     4681 atgtggttca tggtccatgg ctaggtagaa tcgatgatgt gttggacaat gtgactgtct
     4741 tatttgatga tggttccatg tgtaaagttc tacgtgttga acctcttcga cttaaaccta
     4801 ttccgaagaa taatcttgaa gaagatgcta attttcctta ttacccaggg cagcgggtta
     4861 aagcaagctc ctcttcggtt tttaagaatt cgcggtggtt atctggactg tggaagccta
     4921 atcgtttaga aggtacggtg accaaagtta ctgcgggatc aatttttgtc tactggattg
     4981 cctcagctgg ctttggtcca gattcttctg tttccccacc tgaggagcag aatccaagta
     5041 atttgacatt gttgtcatgt ttcactcatg ccaattggca agtgggtgac tggtgccttc
     5101 ttccatctct gaatcaatct gctacaatcc ccttacacaa gcatgtatct aaattaagac
     5161 tttatgattc ccaagctgat cggcaacaga aaattgggcg tgatttagaa gatgtgcaag
     5221 atgaagtaag tggaaaagtt gaaccagctg gtattactgc tgaagctttg ccaaaagtaa
     5281 ctagtgacga tcctcctcaa agaaatcctt ctgtttcaaa ggagcctgtt catgagcctt
     5341 ggcctctcca tcggaagaag attcgcaaac ttgttatcag aaaggacaaa aaggttaaga
     5401 aaaaagagga aagctttgaa caagctcttt tggtagttaa tagcagaaca cgcgttgatg
     5461 tatcctggca ggatggtaca atagaatgta gacgggaagc gataacattg attccaattg
     5521 agactcctgg tgatcatgaa tttgtctctg agcagtatgt ggtagagagg acctctgatg
     5581 atggtgataa cacaactgaa cctagacgtg ctggcgttgt aaaaaatgtc aatgcaaaag
     5641 atcgtactgc ttctgtgagg tggctaaatc cacttcgaag ggcagaagaa ccccgtgagt
     5701 ttgaaaagga agaaattgtc agtgtgtatg agctagaggg ccatccagat tatgactact
     5761 gttatgggga tgttgttgtt cgcttatcac ctattgccgt tgcgttacca gcatcttctc
     5821 ccggaaactc ttttgaggag gcaacacagc aagacaatgg ataccaagat tcagaatccc
     5881 atcaagaagc taaaatcctt gtggataagg aagaaaacga gcctagtacg gacctttcaa
     5941 agctctcatg ggttggaaat attactggcc tcaaggatgg tgatattgaa gtaacatggg
     6001 ccgatggaac aatatcaacg gttggccctc atgcagttta tgtagttgga cgcgatgatg
     6061 atgatgaatc agttgctgga gaaagtgaaa caagtgatgc tgctagttgg gaaactttaa
     6121 acgatgatga taggggtgct cctgagattc ctgaagagga ccttggaagg agtagttcca
     6181 ttgagggaaa ctctgatgca gatatctacg ctgagaatga ttctgggagg aacggtgccc
     6241 tagctcttcc tctggctgca atcgaatttg tgactcgact ggctagtgga atcttttcgc
     6301 gtgcaaggaa atctgtagat tcttccagtt ctgattacac ggttgaaaat gtatataagc
     6361 aagctgaatc gaccaacccg tccgatgaaa cagattccct tgatgaccct agtccttcaa
     6421 aagttaatgt gactgataat tgcgagtcaa agggcactca agcaaatgcc aagaatattt
     6481 tgagcgggga aacctctaca tttttagaag atgaagataa gccagtacct tctgaaggtg
     6541 atagttgcag tttcagacgc tttgatatat cacaagatcc tctggaccac catttccttg
     6601 gcgtagatgg gcagaaaacc aaagaaagac agtggttcaa gaaggttgat caagactgga
     6661 aaatacttca gaacaatctt ccagatggaa tctttgttcg agcttacgaa gatagaatgg
     6721 atctcttgag ggcagtaata gtcggggcat ttggaacacc ataccaagat ggactcttct
     6781 tttttgattt tcaccttcca tctgactacc ctagtgtgcc accgtcggca tattatcatt
     6841 ctggtggatg gaggttaaac cccaatctgt acgaagaagg caaagtctgt cttagccttc
     6901 tcaatacctg gacaggcagg ggaaatgaag tctgggatcc caaatcatct agcatccttc
     6961 aagttcttgt ttcactccag ggattggtgt tgaattcaaa gccttatttc aatgaagccg
     7021 ggtatgataa gcaggtcgga actgcggaag gagaaaaaaa ctcactggga tacaacgaga
     7081 atactttctt gctaaattgt aaaaccatga tgtatcttat gcggaaacca ccaaaggatt
     7141 ttgaagaact catcaaagat catttcagaa aacgcggata ttacattttg aaggcatgtg
     7201 acgcctacat gaaaggatat ctcataggtt ccctcaccaa agatgcctcg gttatcgatg
     7261 aacgcagcag cgctaattca acttcagttg gttttaagct tatgctggct aagatagcac
     7321 caaagctttt ctcagcactg agcgaggtag gagctgactg caatgaattc cagcatctgc
     7381 agcagcaata agcttttacg cttttaagcc agccccgaca cccgccaaca cccgctgacg
     7441 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgggatga
     7501 gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa
     7561 taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga
     7621 ggaactatat ccggattggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     7681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     8041 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     8101 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8161 acattcaaat atgtatccgc tcatgaatta attcttagaa aaactcatcg agcatcaaat
     8221 gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa agccgtttct
     8281 gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc tggtatcggt
     8341 ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg tcaaaaataa
     8401 ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat ggcaaaagtt
     8461 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
     8521 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagtcga aatacgcgat
     8581 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
     8641 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
     8701 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
     8761 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
     8821 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
     8881 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
     8941 ataaatcagc atccatgttg gaatttaatc gcggcctaga gcaagacgtt tcccgttgaa
     9001 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
     9061 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     9121 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     9181 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     9241 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     9301 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     9361 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     9421 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     9481 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     9541 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     9601 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     9661 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     9721 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     9781 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     9841 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     9901 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcaatggt
     9961 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
    10021 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
    10081 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgact ccgggagctg
    10141 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc
    10201 atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt
    10261 gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt
    10321 tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat
    10381 gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg
    10441 gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa
    10501 aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag
    10561 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt
    10621 ttccagactt tacgaaacac ggaaacccaa gaccattcat gttgttgctc aggtcgcaga
    10681 cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc
    10741 agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac
    10801 ccgtggggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg
    10861 accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc
    10921 gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg
    10981 cacctgtcct acgagttgca tgataaagaa cacagtcata agtgcggcga cgatagtcat
    11041 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
    11101 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctcac tgcccgcttt
    11161 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
    11221 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgacacg ggcaacagct
    11281 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc
    11341 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtcct
    11401 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa
    11461 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga
    11521 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt
    11581 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac
    11641 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca
    11701 atgcgaccag atgctccacg cccagtcgcg taccgtcctc atgggagaaa ataatactgt
    11761 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt
    11821 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt
    11881 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg
    11941 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg
    12001 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg
    12061 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt
    12121 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat
    12181 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc
    12241 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg cgccattcga
    12301 tggtgtccgg gatctcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt
    12361 agtaggttga ggccgttgag caccgcc
//
