LOCUS       sequence-205522-       11802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11802)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 11802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-315
FEATURES             Location/Qualifiers
     source          1..11802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     963..982
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(1002..1079)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1285..1304
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1388..1407
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1388..1406
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1407..1431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1485..1502
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5050..5139
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5152..5241
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(8124..8142)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      8138..8185
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      8222..8677
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8309..8328)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8519..8540
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(8769..9584)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(8769..9584)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     9492..9511
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     9576..9595
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      9706..10294
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10195..10214
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10448..10465
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10480..10619
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10545..10564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10705..10727
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(10721..10912)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(11721..11802,1..1001))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gtattgggcg ccagggtggt ttttcttttc accagtgaca cgggcaacag ctgattgccc
       61 ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg ccccagcagg
      121 cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc ctcggtatcg
      181 tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt aatggcgcgc
      241 attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac gatgccctca
      301 ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc ttcccgttcc
      361 gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag acgcagacgc
      421 gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc caatgcgacc
      481 agatgctcca cgcccagtcg cgtaccgtcc tcatgggaga aaataatact gttgatgggt
      541 gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc ttccacagca
      601 atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg ttgcgcgaga
      661 agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat cgacaccacc
      721 acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg cgacggcgcg
      781 tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc cgccagttgt
      841 tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac tttttcccgc
      901 gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg ataagagaca
      961 ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac cctgaattga
     1021 ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc gatggtgtcc
     1081 gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt
     1141 gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag
     1201 tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa
     1261 gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc
     1321 tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc
     1381 gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa
     1441 ataattttgc cttaacttta agaaggagat ataccatggg cagccaccac caccaccacc
     1501 acagcggaat gcagatcttt gttaagactc tcaccggaaa gacaatcacc ctagaggtgg
     1561 aaagctccga caccatcgac aacgttaagg ccaagatcca ggataaggag ggcattcctc
     1621 cggatcagca gaggcttatt ttcgccggca agcagctaga ggatggccgt acgttggctg
     1681 attacaatat ccagaaggaa tccaccctcc acttggtcct caggctccgt ggtggttaag
     1741 cttgcaatta actttaagaa ggagatatac aatgcttcac aagcgagcta gtgaagctaa
     1801 cgataagaac gataatacca tcatcggttc cgatttagca tcatctaaga aacgccgtat
     1861 cgatttcact gaatcttcgt ctgataagtc atcttcgatt ttagcttctg gtagtagcag
     1921 gggttttcac ggcgatagcg tcgttcagca aatcgacatg gcttttggta attcgaaccg
     1981 tcaggagatt gatgaagatc tgcacagtcg ccagctcgcc gtctatggtc gtgagactat
     2041 gaggcgtctc tttgcttcca atgttctcat ctcggggatg cacggtcttg gtgccgagat
     2101 tgccaagaat cttatacttg ctggtgtgaa gtctgtgacc ttgcatgatg aaagagttgt
     2161 agagctatgg gacttatcaa gcaactttgt tttctcggag gatgatgttg gcaagaatag
     2221 agcagatgct tctgttcaga agttacagga tcttaacaat gctgtggttg tctctagctt
     2281 gaccaaaagc ttaaacaaag aggatctttc tggcttccag gttgttgtat tctctgacat
     2341 aagcatggaa agagcaatcg aatttgatga ctattgtcac agccatcagc ctcctatcgc
     2401 ttttgtcaag gctgatgtca ggggtctttt cggctctgtc ttttgtgatt ttgggcctga
     2461 atttgcggtt cttgatgttg atggagagga accccataca ggcatcattg cgtctatctc
     2521 taatgagaac caggcattta tttcctgtgt cgacgatgag aggcttgagt ttgaagatgg
     2581 tgacctagtt gttttctcag aagttgaggg tatgacagaa ttgaatgatg ggaaaccaag
     2641 gaagataaaa agtacacggc catattcatt cacccttgat gaggacacta ctaactatgg
     2701 aacatatgtg aagggtggta ttgtcactca agtgaaacag ccaaagttac tgaatttcaa
     2761 gccattgagg gaagcgctta aggatccagg ggattttcta tttagtgatt tctctaagtt
     2821 tgatcggcct ccactccttc acttagcatt ccaggcactt gatcatttta aagctgaagc
     2881 tggcagattt cctgttgctg gctcagaaga ggacgctcag aagcttatat cgattgccac
     2941 agccatcaat accgggcagg gtgatttaaa ggtggagaat gttgatcaga agcttctaag
     3001 acacttttcc tttggagcca aggctgtcct gaatcctatg gctgcaatgt ttggcggtat
     3061 tgttggacag gaggttgtta aagcttgctc tggaaaattt catcctctct tccagttttt
     3121 ctactttgat tcagtggagt cactcccctc tgaacctgtg gactcgagtg actttgcacc
     3181 aagaaacagc cggtatgatg cccaaatatc tgtatttggg gccaagttcc agaagaaact
     3241 tgaagatgct aaagttttca cagtagggtc tggtgctctt ggctgtgagt tcttgaaaaa
     3301 tttggctctg atgggggttt cttgtggaag ccaagggaag ctgacagtga cagatgatga
     3361 tatcattgag aagagtaacc tcagtcgtca atttctgttc cgtgactgga acattggaca
     3421 ggctaaatcc acagttgctg cttcggctgc tgctgttata aatcctcgct tcaacattga
     3481 ggccctgcaa aaccgtgttg gcgcggagac tgagaatgta tttgacgatg ccttctggga
     3541 gaacttaact gttgtcgtca atgcactgga taatgttaat gcgaggctct atgttgattc
     3601 gaggtgcttg tatttccaga agcctctcct tgagtctgga actcttggta caaagtgcaa
     3661 cacacagtcg gttatcccac atctaactga aaattacggt gcctcaaggg acccaccaga
     3721 gaaacaggcc cccatgtgta cagtgcattc cttcccgcat aacattgatc actgtttaac
     3781 ttgggctcgc tctgagtttg agggtctgct ggagaaaact cccgctgaag tgaatgcata
     3841 tctttctagc ccagttgagt acactaactc aatgatgagt gctggtgatg ctcaggcgag
     3901 ggacacattg gagaggattg ttgagtgcct tgaaaaggag aagtgtgaaa ccttccaaga
     3961 ctgcttaacc tgggcacgac tgaggtttga ggattacttt gtgaaccgtg tcaagcaatt
     4021 gatatacaca tttcctgaag atgctgcgac tagtaccgga gctccattct ggtctgctcc
     4081 aaaaagattc cctcgtccac tccagtactc gtcgtccgac ccaagtctcc ttaactttat
     4141 cacagcgact gctattttac gagcagagac atttgggatc ccgatacctg agtggactaa
     4201 gaacccaaag gaagcagcag aagctgttga cagagtgata gtcccagact ttgagccaag
     4261 gcaagatgca aagattgtga ctgatgagaa agctaccact ttaaccactg cttcggtgga
     4321 cgacgctgca gtcatcgacg atctcattgc taagatcgac cagtgtaggc ataacttgtc
     4381 gccagacttc aggatgaaac ctattcagtt cgaaaaggat gatgatacaa actatcacat
     4441 ggatgtgata gcaggactag ccaacatgag ggcgagaaat tacagcatcc ctgaagtgga
     4501 caagctgaaa gcaaagttca tagcagggag aatcatccca gccattgcga cttcaacagc
     4561 catggctact gggctggtct gcctcgagct gtacaaggtc cttgatggag gacataaagt
     4621 ggaagcttac agaaacacat ttgccaacct ggcacttcca ctattctcca tggctgaacc
     4681 acttccacca aaggtggtga agcaccgtga catggcttgg acagtttggg acagatgggt
     4741 tctcaaggga aacccaactc ttcgtgaggt gttgcagtgg ctggaggaca aagggcttag
     4801 cgcatacagc atctcttgtg gaagctgtct actgttcaac agtatgttca caaggcacaa
     4861 ggagaggatg gacaagaagg ttgtggatct cgctcgggat gttgcgaaag tcgagttacc
     4921 gccttatcgc aaccatcttg atgtagtggt ggcttgtgag gatgaagatg acaatgacgt
     4981 cgatattcct ctcgtctcta tctacttcag gtgagcttac tattaacttt aagaaggaga
     5041 tataccatgt acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac
     5101 tatgcaggat tgtatccata tgacgttcca gattacgcca ctagagctgc ttacccatac
     5161 gatgttcctg actatgcggg ctatccctat gacgtcccgg actatgcagg attgtatcca
     5221 tatgacgttc cagattacgc aatggaaatg tcccttactg actctgattg ggatagctcc
     5281 agcgacagtg gtagcagtga acacgaagaa gtcgagtttt cttatggtgg acgggcacag
     5341 aacattttct caaaccttga agaaaccatt ggcaaaatcg atgagttcct gtcgttcgag
     5401 aggggattta tgtatggtga cattgtgcgg tccgccactg aaccatcagg acagagtggc
     5461 agggttatca acatagacat gtttgtcaat ctcgaaagta ctcatgggaa gatcatgaag
     5521 gaagttgata ccaagaggct tcaaaagttg cgttctattt cactctctga ttatgtgatt
     5581 aacggacctt gggttggaag ggttgacaaa atagttgagc gtgtctctgt cacccttgat
     5641 gatgggacca actatgaggt ccttgtagat ggtcaagata aacttgtggc cattccccca
     5701 aatttactcg aggattctca atattcgtat tacccagggc aaagagttca ggtaaagctg
     5761 gcccatgccc ccagatcaac tacatggtta tgcgggacct ggagaggaac ccaggttatg
     5821 ggaactgttt gcactgtaga agcaggactt gtctacgtcg attgggttgc ctccatcgta
     5881 atggagggtg atcggaattt aactgcacct caagctttgc agaatcctga gagtttaact
     5941 ttgttacctt gtgtttctca tgcgagttgg cagcttggtg actggtgtat actccctggc
     6001 tcttcccact gtgatatagc agagcggcaa actccaaatg tggctgccta caatctcaat
     6061 gaatgccata agacattcca aaaagggttt aacagaaata tgcagaactc aggtttggat
     6121 gagctatttg ttatcacaaa gacaaagatg aaggttgctg ttatgtggca agatggtagc
     6181 tgcagtctgg gagttgattc ccaacagctg cttcctgttg gtgctgttaa tgctcatgat
     6241 ttttggcccg aacagtttgt tgtggaaaag gaaacctgca acagcaaaaa atggggagtt
     6301 gtgaaggctg tcaatgctaa ggaacaaact gtgaaggtac aatggacaat acaggttgag
     6361 aaagaagcaa caggttgtgt tgatgaggtg atggaagaaa ttgtcagtgc atatgaactg
     6421 cttgagcacc ctgattttgg attctgtttc agcgatgtgg tggtcaagtt acttccagaa
     6481 ggaaaatttg atccaaatgc agacacaatc gtcgctacag aggcgaaaca cctacttaca
     6541 gagagtgact acagtggcgc atattttttg tcaagtattg gtgttgttac aggttttaaa
     6601 aatggttccg tgaaggtgaa atgggccaat ggttctacta gcaaggttgc accatgtgaa
     6661 atttggaaaa tggaaaggtc tgaatattcc aactctagca ctgtaagttc ggaaggcagt
     6721 gttcaagatc taagtcagaa gattagtcaa tcggatgaag catcttcaaa ccatcaggaa
     6781 acgggtctgg tgaagctcta cagtgttggt gaaagttgca acgagaacat tccggaatgt
     6841 agttcatttt tccttccaaa agctgccatt ggatttatca caaaccttgc atcaagcctt
     6901 tttggttatc agggttccac ttcggttata agttcacatt cacgttgcaa tgattctgaa
     6961 gatcaaagtg actctgaggt ccttgttcaa gaaacagcag aatcatatga caactctgaa
     7021 acaaattcag gtgaagtgga tatgaccacc acgatggtca acatacctat agaaggaaaa
     7081 ggaattaaca agacactgga ttcaactctt ctagagaaca gcaggaacca agtgagattc
     7141 agacagtttg atatggttaa tgactgctcg gaccatcatt tcctttcttc ggataaaggg
     7201 ttggctcagt cccaggtcac aaagagttgg gtgaagaaag tccagcaaga atggagcaat
     7261 ttggaggcaa atcttccgaa cacgatatat gtgcgtgtgt gtgaagaaag gatggacctt
     7321 ctgcgtgcag ccctggttgg tgcccctgga acgccatatc acgatgggct tttctttttc
     7381 gacataatgc ttccacctca atatcctcat gagccaccaa tggtacatta tcattcaggt
     7441 gggatgcgac tgaatccgaa cctgtatgag tcaggaagag tttgcttgag tctgctgaat
     7501 acatggagtg gctctggcac tgaagtatgg aacgcaggga gctcctccat ccttcaactt
     7561 cttctttcgt ttcaggctct ggttctgaat gagaagcctt acttcaatga agctggctat
     7621 gataagcagt tgggccgagc cgagggagag aaaaactcag tgagttacaa tgagaatgca
     7681 ttcctcataa cctgcaaatc catgatctca atgctccgta agcctccaaa gcattttgag
     7741 atgcttgtga aggaccattt tacgcaccgg gcccagcatg ttctggctgc gtgcaaggct
     7801 tatatggaag gcgtccctgt aggatcatca gctaacctgc aggggaactc aaccacaaat
     7861 tccaccggtt tcaagatcat gctctccaaa ctctacccaa aacttcttga agcattctca
     7921 gagattggag ttgattgcgt ccaagagatt ggaccagaat cataagcttt tacgctttta
     7981 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     8041 gcatccgctt acagacaagc tgtgaccggg atgagatccg gctgctaaca aagcccgaaa
     8101 ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc
     8161 taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat tggcgaatgg
     8221 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8281 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8341 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8401 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8461 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8521 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8581 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8641 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8701 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8761 attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     8821 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     8881 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     8941 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     9001 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     9061 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     9121 cgtgattgcg cctgagcgag tcgaaatacg cgatcgctgt taaaaggaca attacaaaca
     9181 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     9241 tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt ggtgagtaac
     9301 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     9361 agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc tttgccatgt
     9421 ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt cgcacctgat
     9481 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     9541 aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     9601 ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac gtgagttttc
     9661 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     9721 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     9781 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     9841 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     9901 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     9961 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    10021 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    10081 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    10141 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    10201 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    10261 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    10321 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    10381 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    10441 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
    10501 tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat ctgctctgat
    10561 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
    10621 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
    10681 cttacagaca agctgtgacc gactccggga gctgcatgtg tcagaggttt tcaccgtcat
    10741 caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac
    10801 agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct
    10861 ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct
    10921 ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc
    10981 tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac
    11041 aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct
    11101 tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc
    11161 ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac
    11221 ccaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg
    11281 ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc
    11341 gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg ccggcgataa
    11401 tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg
    11461 cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc
    11521 ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa
    11581 agaacacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga
    11641 ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga gtgagctaac
    11701 ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
    11761 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gc
//
