LOCUS       sequence-205523-        6354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses E. coli HPII catalase with N-terminal 6x his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Mehl R, van Zee K, Kean K
  TITLE     
  JOURNAL   2020
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "2020"
COMMENT     Sequence Label: pBad-HPII
FEATURES             Location/Qualifiers
     source          1..6354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      14..602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     503..522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     756..773
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    788..928
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(854..873)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1014..1036
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(1192..2070)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2097..2381
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     2304..2323
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             2427..2444
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2448..2480
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(2484..2492,2493..2507)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(4851..4868)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      5021..5107
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5199..5226
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        5245..5336
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5337..5405,5406..6197)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5555..5574)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
       61 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
      121 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
      181 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
      241 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
      301 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
      361 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
      421 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
      481 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
      541 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
      601 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
      661 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
      721 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
      781 aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat
      841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
      901 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
      961 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
     1021 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc agatcaattc
     1081 gcgcgcgaag gcgaagcggc atgcataatg tgcctgtcaa atggacgaag cagggattct
     1141 gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa
     1201 cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc
     1261 cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac caacattgcg
     1321 accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct ggctgatacg
     1381 ttggtcctcg cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag
     1441 acgcgacggc gacaagcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc
     1501 caggtgatcg ctgatgtact gacaagcctc gcgtacccga ttatccatcg gtggatggag
     1561 cgactcgtta atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag
     1621 cagctccgaa tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct
     1681 gaaatgcggc tggtgcgctt catccgggcg aaagaacccc gtattggcaa atattgacgg
     1741 ccagttaagc cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca
     1801 ttcgcgagcc tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat
     1861 atcacccggt cggcaaacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat
     1921 ggtgagattg agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata
     1981 accgttggcc tcaatcggcg ttaaacccgc caccagatgg gcattaaacg agtatcccgg
     2041 cagcagggga tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga
     2101 aaccaattgt ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc
     2161 gctaaccaaa ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc
     2221 catgacaaaa acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta
     2281 tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc
     2341 tacctgacgc tttttatcgc aactctctac tgtttctcca tacccgtttt ttgggctaac
     2401 aggaggaatt aaccatgggg ggttctcatc atcatcatca tcatggtatg gctagcatga
     2461 ctggtggaca gcaaatgggt cgggatctgt acgacgatga cgataaggat cgatggggat
     2521 ccgagctcga gtcgcaacat aacgaaaaga acccacatca gcaccagtca ccactacacg
     2581 attccagcga agcgaaaccg gggatggact cactggcacc tgaggacggc tctcatcgtc
     2641 cagcggctga accaacaccg ccaggtgcac aacctaccgc cccagggagc ctgaaagccc
     2701 ctgatacgcg taacgaaaaa cttaattctc tggaagacgt acgcaaaggc agtgaaaatt
     2761 atgcgctgac cactaatcag ggcgtgcgca tcgccgacga tcaaaactca ctgcgtgccg
     2821 gtagccgtgg tccaacgctg ctggaagatt ttattctgcg cgagaaaatc acccactttg
     2881 accatgagcg cattccggaa cgtattgttc atgcacgcgg atcagccgct cacggttatt
     2941 tccagccata taaaagctta agcgatatta ccaaagcgga tttcctctca gatccgaaca
     3001 aaatcacccc agtatttgta cgtttctcta ccgttcaggg tggtgctggc tctgctgata
     3061 ccgtgcgtga tatccgtggc tttgccacca agttctatac cgaagagggt atttttgacc
     3121 tcgttggcaa taacacgcca atcttcttta tccaggatgc gcataaattc cccgattttg
     3181 ttcatgcggt aaaaccagaa ccgcactggg caattccaca agggcaaagt gcccacgata
     3241 ctttctggga ttatgtttct ctgcaacctg aaactctgca caacgtgatg tgggcgatgt
     3301 cggatcgcgg catcccccgc agttaccgca ccatggaagg cttcggtatt cacaccttcc
     3361 gcctgattaa tgccgaaggg aaggcaacgt ttgtacgttt ccactggaaa ccactggcag
     3421 gtaaagcctc actcgtttgg gatgaagcac aaaaactcac cggacgtgac ccggacttcc
     3481 accgccgcga gttgtgggaa gccattgaag caggcgattt tccggaatac gaactgggct
     3541 tccagttgat tcctgaagaa gatgaattca agttcgactt cgatcttctc gatccaacca
     3601 aacttatccc ggaagaactg gtgcccgttc agcgtgtcgg caaaatggtg ctcaatcgca
     3661 acccggataa cttctttgct gaaaacgaac aggcggcttt ccatcctggg catatcgtgc
     3721 cgggactgga cttcaccaac gatccgctgt tgcagggacg tttgttctcc tataccgata
     3781 cacaaatcag tcgtcttggt gggccgaatt tccatgagat tccgattaac cgtccgacct
     3841 gcccttacca taatttccag cgtgacggca tgcatcgcat ggggatcgac actaacccgg
     3901 cgaattacga accgaactcg attaacgata actggccgcg cgaaacaccg ccggggccga
     3961 aacgcggcgg ttttgaatca taccaggagc gcgtggaagg caataaagtt cgcgagcgca
     4021 gcccatcgtt tggcgaatat tattcccatc cgcgtctgtt ctggctaagt cagacgccat
     4081 ttgagcagcg ccatattgtc gatggtttca gttttgagtt aagcaaagtc gttcgtccgt
     4141 atattcgtga gcgcgttgtt gaccagctgg cgcatattga tctcactctg gcccaggcgg
     4201 tggcgaaaaa tctcggtatc gaactgactg acgaccagct gaatatcacc ccacctccgg
     4261 acgtcaacgg tctgaaaaag gatccatcct taagtttgta cgccattcct gacggtgatg
     4321 tgaaaggtcg cgtggtagcg attttactta atgatgaagt gagatcggca gaccttctgg
     4381 ccattctcaa ggcgctgaag gccaaaggcg ttcatgccaa actgctctac tcccgaatgg
     4441 gtgaagtgac tgcggatgac ggtacggtgt tgcctatagc cgctaccttt gccggtgcac
     4501 cttcgctgac ggtcgatgcg gtcattgtcc cttgcggcaa tatcgcggat atcgctgaca
     4561 acggcgatgc caactactac ctgatggaag cctacaaaca ccttaaaccg attgcgctgg
     4621 cgggtgacgc gcgcaagttt aaagcaacaa tcaagatcgc tgaccagggt gaagaaggga
     4681 ttgtggaagc tgacagcgct gacggtagtt ttatggatga actgctaacg ctgatggcag
     4741 cacaccgcgt gtggtcacgc attcctaaga ttgacaaaat tcctgcctga tggggtacca
     4801 tatgggaatt cgaagcttgg ctgttttggc ggatgagaga agattttcag cctgatacag
     4861 attaaatcag aacgcagaag cggtctgata aaacagaatt tgcctggcgg cagtagcgcg
     4921 gtggtcccac ctgaccccat gccgaactca gaagtgaaac gccgtagcgc cgatggtagt
     4981 gtggggtctc cccatgcgag agtagggaac tgccaggcat caaataaaac gaaaggctca
     5041 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag
     5101 gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc
     5161 aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc tgacggatgg
     5221 cctttttgcg tttctacaaa ctcttttgtt tatttttcta aatacattca aatatgtatc
     5281 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5341 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5401 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5461 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5521 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     5581 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5641 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5701 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5761 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5821 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5881 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5941 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     6001 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     6061 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     6121 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     6181 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6241 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6301 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aaga
//
