LOCUS       sequence-205525-       10908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10908)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-316
FEATURES             Location/Qualifiers
     source          1..10908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     584..603
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(623..700)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     906..925
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1009..1028
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1009..1027
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1028..1052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1106..1123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4671..4760
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4773..4862
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6851..6869)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6865..6912
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6949..7404
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7036..7055)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7246..7267
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7496..8311)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7496..8311)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8219..8238
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8303..8322
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      8433..9021
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8922..8941
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9175..9192
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9207..9346
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9272..9291)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9432..9454
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(9448..9639)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(10448..10908,1..622))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
       61 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
      121 gcgtaccgtc ctcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
      181 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
      241 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
      301 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
      361 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
      421 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
      481 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
      541 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
      601 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
      661 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc
      721 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
      781 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
      841 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
      901 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
      961 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
     1021 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg ccttaacttt
     1081 aagaaggaga tataccatgg gcagccacca ccaccaccac cacagcggaa tgcagatctt
     1141 tgttaagact ctcaccggaa agacaatcac cctagaggtg gaaagctccg acaccatcga
     1201 caacgttaag gccaagatcc aggataagga gggcattcct ccggatcagc agaggcttat
     1261 tttcgccggc aagcagctag aggatggccg tacgttggct gattacaata tccagaagga
     1321 atccaccctc cacttggtcc tcaggctccg tggtggttaa gcttgcaatt aactttaaga
     1381 aggagatata caatgcttca caagcgagct agtgaagcta acgataagaa cgataatacc
     1441 atcatcggtt ccgatttagc atcatctaag aaacgccgta tcgatttcac tgaatcttcg
     1501 tctgataagt catcttcgat tttagcttct ggtagtagca ggggttttca cggcgatagc
     1561 gtcgttcagc aaatcgacat ggcttttggt aattcgaacc gtcaggagat tgatgaagat
     1621 ctgcacagtc gccagctcgc cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc
     1681 aatgttctca tctcggggat gcacggtctt ggtgccgaga ttgccaagaa tcttatactt
     1741 gctggtgtga agtctgtgac cttgcatgat gaaagagttg tagagctatg ggacttatca
     1801 agcaactttg ttttctcgga ggatgatgtt ggcaagaata gagcagatgc ttctgttcag
     1861 aagttacagg atcttaacaa tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa
     1921 gaggatcttt ctggcttcca ggttgttgta ttctctgaca taagcatgga aagagcaatc
     1981 gaatttgatg actattgtca cagccatcag cctcctatcg cttttgtcaa ggctgatgtc
     2041 aggggtcttt tcggctctgt cttttgtgat tttgggcctg aatttgcggt tcttgatgtt
     2101 gatggagagg aaccccatac aggcatcatt gcgtctatct ctaatgagaa ccaggcattt
     2161 atttcctgtg tcgacgatga gaggcttgag tttgaagatg gtgacctagt tgttttctca
     2221 gaagttgagg gtatgacaga attgaatgat gggaaaccaa ggaagataaa aagtacacgg
     2281 ccatattcat tcacccttga tgaggacact actaactatg gaacatatgt gaagggtggt
     2341 attgtcactc aagtgaaaca gccaaagtta ctgaatttca agccattgag ggaagcgctt
     2401 aaggatccag gggattttct atttagtgat ttctctaagt ttgatcggcc tccactcctt
     2461 cacttagcat tccaggcact tgatcatttt aaagctgaag ctggcagatt tcctgttgct
     2521 ggctcagaag aggacgctca gaagcttata tcgattgcca cagccatcaa taccgggcag
     2581 ggtgatttaa aggtggagaa tgttgatcag aagcttctaa gacacttttc ctttggagcc
     2641 aaggctgtcc tgaatcctat ggctgcaatg tttggcggta ttgttggaca ggaggttgtt
     2701 aaagcttgct ctggaaaatt tcatcctctc ttccagtttt tctactttga ttcagtggag
     2761 tcactcccct ctgaacctgt ggactcgagt gactttgcac caagaaacag ccggtatgat
     2821 gcccaaatat ctgtatttgg ggccaagttc cagaagaaac ttgaagatgc taaagttttc
     2881 acagtagggt ctggtgctct tggctgtgag ttcttgaaaa atttggctct gatgggggtt
     2941 tcttgtggaa gccaagggaa gctgacagtg acagatgatg atatcattga gaagagtaac
     3001 ctcagtcgtc aatttctgtt ccgtgactgg aacattggac aggctaaatc cacagttgct
     3061 gcttcggctg ctgctgttat aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt
     3121 ggcgcggaga ctgagaatgt atttgacgat gccttctggg agaacttaac tgttgtcgtc
     3181 aatgcactgg ataatgttaa tgcgaggctc tatgttgatt cgaggtgctt gtatttccag
     3241 aagcctctcc ttgagtctgg aactcttggt acaaagtgca acacacagtc ggttatccca
     3301 catctaactg aaaattacgg tgcctcaagg gacccaccag agaaacaggc ccccatgtgt
     3361 acagtgcatt ccttcccgca taacattgat cactgtttaa cttgggctcg ctctgagttt
     3421 gagggtctgc tggagaaaac tcccgctgaa gtgaatgcat atctttctag cccagttgag
     3481 tacactaact caatgatgag tgctggtgat gctcaggcga gggacacatt ggagaggatt
     3541 gttgagtgcc ttgaaaagga gaagtgtgaa accttccaag actgcttaac ctgggcacga
     3601 ctgaggtttg aggattactt tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa
     3661 gatgctgcga ctagtaccgg agctccattc tggtctgctc caaaaagatt ccctcgtcca
     3721 ctccagtact cgtcgtccga cccaagtctc cttaacttta tcacagcgac tgctatttta
     3781 cgagcagaga catttgggat cccgatacct gagtggacta agaacccaaa ggaagcagca
     3841 gaagctgttg acagagtgat agtcccagac tttgagccaa ggcaagatgc aaagattgtg
     3901 actgatgaga aagctaccac tttaaccact gcttcggtgg acgacgctgc agtcatcgac
     3961 gatctcattg ctaagatcga ccagtgtagg cataacttgt cgccagactt caggatgaaa
     4021 cctattcagt tcgaaaagga tgatgataca aactatcaca tggatgtgat agcaggacta
     4081 gccaacatga gggcgagaaa ttacagcatc cctgaagtgg acaagctgaa agcaaagttc
     4141 atagcaggga gaatcatccc agccattgcg acttcaacag ccatggctac tgggctggtc
     4201 tgcctcgagc tgtacaaggt ccttgatgga ggacataaag tggaagctta cagaaacaca
     4261 tttgccaacc tggcacttcc actattctcc atggctgaac cacttccacc aaaggtggtg
     4321 aagcaccgtg acatggcttg gacagtttgg gacagatggg ttctcaaggg aaacccaact
     4381 cttcgtgagg tgttgcagtg gctggaggac aaagggctta gcgcatacag catctcttgt
     4441 ggaagctgtc tactgttcaa cagtatgttc acaaggcaca aggagaggat ggacaagaag
     4501 gttgtggatc tcgctcggga tgttgcgaaa gtcgagttac cgccttatcg caaccatctt
     4561 gatgtagtgg tggcttgtga ggatgaagat gacaatgacg tcgatattcc tctcgtctct
     4621 atctacttca ggtgagctta ctattaactt taagaaggag atataccatg tacccatacg
     4681 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga ttgtatccat
     4741 atgacgttcc agattacgcc actagagctg cttacccata cgatgttcct gactatgcgg
     4801 gctatcccta tgacgtcccg gactatgcag gattgtatcc atatgacgtt ccagattacg
     4861 caatggagcc caacgtagtg gagatcgcta ctcctcctgc cgcttcttgc tcgagaattc
     4921 gaacgccaac aaaggctgaa actccagaag tgattgatgt ggaggaatat gatttgcaaa
     4981 acggtggtgt tcctaatggc aacaatgttg attataagaa caaagggaag gctatagact
     5041 ttgattccat gtcttacggt gactatggtg aagaagatga gtatgctgtg ggatctcctg
     5101 gtgatgatta tggttatcct gaatcatctc cactatcaaa tagtttgtta gacccagagt
     5161 ctttgatcta tgaggacgac gaaaactatt ccgaacaata tgactttgaa atggaggcgg
     5221 agcctgataa ttactccatg tatcaagacc ttttcgatgg taaggatatc ccaactggtg
     5281 tggaggtttc aatggattgg tttccaaatt cagctgacaa ggaatcagcg agtagtagca
     5341 aatcgtctca tgctaataac gggaataaca gttccaagaa agcaacaaaa gcaagtggaa
     5401 tccattctca attttcttca gatatggaaa cccctgtagc tcaaccatgg aatgctttac
     5461 cgcacaaagc tgaaggagtc ataccaaact ctgcttatgc cttgccacag aacagcaagg
     5521 cttttcaacc accttacgct gtccactatt cagcccttaa aactgctttt tccaattatc
     5581 ttcagcctca gactcctgat accgtattgg gggaggctcc agctcctgct gcaggttctt
     5641 ctggtttatt gccaccaaac acacccggtt ttaaaagtaa tgctgctcgg tttaaagagg
     5701 agccgcctat attacctccg gatgattcca gagttaagag aaatatggag gattatcttg
     5761 ggctttattt gtttttcaaa agatttgaca tcgtcgaaga tttttcagat caccactatg
     5821 cttctaaggg aacaacttca aagcagcatt caaaggattg ggcgaaaagg atccaagacg
     5881 agtggagaat tcttgagaaa gatttaccag agatgatatt tgtcagagct tatgaatcta
     5941 ggatggatct tttaagggct gtaattatcg gagcccaagg aactccctac catgatggac
     6001 tcttcttttt tgatatcttc ttcccggata catacccttc tacgccaccg attgttcatt
     6061 accattctgg tgggctaaga atcaacccaa atctgtacaa ttgtgggaaa gtgtgtctga
     6121 gtctactcgg cacttggagt ggtaaccaga gggagaaatg gatccccaac acttcgacga
     6181 tgctacaagt tcttgtctcg atccaaggtc taatcttaaa tcaaaaacct tacttcaacg
     6241 agcccggcta cgagaggtca gcaggttctg cacatggtga gagtacctct aaagcttaca
     6301 gcgaaaacac attcatactg tctttaaaga ctatggttta caccatgagg aggccaccca
     6361 agtattttga ggactttgcg tatggacatt tcttcagctg tgctcacgac gtgttgaagg
     6421 cgtgtaatgc ttacagaaac ggagctacac cgggatatct ggtgaagggg gctccagatg
     6481 tggaagagaa tagcgcgggc atgagctctc tgaagtttag gacagatgtg gctacctttg
     6541 tggagacagt gctgttgaaa gagtttattc ttctaggtgt ccttggactc gaaccggaag
     6601 aagaagaaaa aacacctgag actataatcg ttgcagagag tagcaaatgt accagatcaa
     6661 ggtccaagag agacagagtt tcttctagtt gagcttttac gcttttaagc cagccccgac
     6721 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6781 gacaagctgt gaccgggatg agatccggct gctaacaaag cccgaaagga agctgagttg
     6841 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     6901 aggggttttt tgctgaaagg aggaactata tccggattgg cgaatgggac gcgccctgta
     6961 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     7021 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     7081 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     7141 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     7201 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     7261 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     7321 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
     7381 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     7441 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgaatt aattcttaga
     7501 aaaactcatc gagcatcaaa tgaaactgca atttattcat atcaggatta tcaataccat
     7561 atttttgaaa aagccgtttc tgtaatgaag gagaaaactc accgaggcag ttccatagga
     7621 tggcaagatc ctggtatcgg tctgcgattc cgactcgtcc aacatcaata caacctatta
     7681 atttcccctc gtcaaaaata aggttatcaa gtgagaaatc accatgagtg acgactgaat
     7741 ccggtgagaa tggcaaaagt ttatgcattt ctttccagac ttgttcaaca ggccagccat
     7801 tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct
     7861 gagcgagtcg aaatacgcga tcgctgttaa aaggacaatt acaaacagga atcgaatgca
     7921 accggcgcag gaacactgcc agcgcatcaa caatattttc acctgaatca ggatattctt
     7981 ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt gagtaaccat gcatcatcag
     8041 gagtacggat aaaatgcttg atggtcggaa gaggcataaa ttccgtcagc cagtttagtc
     8101 tgaccatctc atctgtaaca tcattggcaa cgctaccttt gccatgtttc agaaacaact
     8161 ctggcgcatc gggcttccca tacaatcgat agattgtcgc acctgattgc ccgacattat
     8221 cgcgagccca tttataccca tataaatcag catccatgtt ggaatttaat cgcggcctag
     8281 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
     8341 cagacagttt tattgttcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     8401 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     8461 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     8521 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     8581 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     8641 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     8701 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     8761 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     8821 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     8881 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     8941 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     9001 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     9061 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     9121 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     9181 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     9241 ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     9301 gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     9361 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     9421 tgtgaccgac tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     9481 gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     9541 ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     9601 gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     9661 atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     9721 tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     9781 gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     9841 tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     9901 gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccca agaccattca
     9961 tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
    10021 cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
    10081 caggagcacg atcatgcgca cccgtggggc cgccatgccg gcgataatgg cctgcttctc
    10141 gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc
    10201 gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa
    10261 aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga acacagtcat
    10321 aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc
    10381 tctcaagggc atcggtcgag atcccggtgc ctaatgagtg agctaactta cattaattgc
    10441 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10501 cggccaacgc gcggggagag gcggtttgcg tattgggcgc cagggtggtt tttcttttca
    10561 ccagtgacac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
    10621 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
    10681 ggatataaca tgagctgtcc tcggtatcgt cgtatcccac taccgagata tccgcaccaa
    10741 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
    10801 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
    10861 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgat
//
