LOCUS       sequence-205528-       13835 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter 
            in arabidopsis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13835)
  AUTHORS   Gallego-Bartolome J, Gardiner J, Liu W, Papikian A, Ghoshal B, Kuo 
            HY, Zhao JM, Segal DJ, Jacobsen SE
  TITLE     Targeted DNA demethylation of the Arabidopsis genome using the human
            TET1 catalytic domain.
  JOURNAL   Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):E2125-E2134. doi: 
            10.1073/pnas.1716945115. Epub 2018 Feb 14.
  PUBMED    29444862
REFERENCE   2  (bases 1 to 13835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1716945115"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2018-02-27- 27"; volume: "115"; issue: "9"; pages: 
            "E2125-E2134"
COMMENT     Sequence Label: pUBQ10::ZF108_3xFLAG_YPet
FEATURES             Location/Qualifiers
     source          1..13835
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      408..602
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(668..1741)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(2170..2799)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    4100..4124
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     4312..4334
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4326..4343
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4327..4343
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             6441..6485
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             6513..6536
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             7047..7118
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     protein_bind    7158..7182
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(7233..7235,7236..7949)
                     /codon_start=1
                     /product="yellow fluorescent protein optimized for FRET 
                     with CyPet (Nguyen and Daugherty, 2005)"
                     /label=YPet
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LLCTTGKLPVPWPTLVTTLGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALFKDPNEKRDHMVLLE
                     FLTAAGITEGMNELYN"
     primer_bind     complement(7536..7555)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     protein_bind    complement(7966..7990)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(8802..8818)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8802..8818)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8815..8837)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8826..8842
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8850..8856,8857..8874,8875..8880))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8895..8916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        9107..9783
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     9720..9740
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             9828..10379
                     /codon_start=1
                     /gene="Streptomyces hygroscopicus bar"
                     /product="phosphinothricin acetyltransferase"
                     /label=BlpR
                     /note="confers resistance to bialophos or phosphinothricin"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     polyA_signal    10386..10560
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    10638..10662
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11087..11881
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      11968..12556
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12457..12476
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12710..12727
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    12742..12882
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(12808..12827)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12968..12990
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(13044..13835)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 aatgtttaac tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa
       61 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg
      121 taccccaaaa aaacagtcat aacaagccat gaaaaccgcc actgcgccgt taccaccgct
      181 gcgttcggtc aaggttctgg accagttgcg tgagcgcatg gcgccggcgg tcgagtggcg
      241 acggcgcggc ttgtccgcgc cctggtagat tgcctggccg taggccagcc atttttgagc
      301 ggccagcggc cgcgataggc cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa
      361 gggtaggcgc tttttgcagc tcttcggctg tgcgctggcc agacagttat gcacaggcca
      421 ggcgggtttt aagagtttta ataagtttta aagagtttta ggcggaaaaa tcgccttttt
      481 tctcttttat atcagtcact tacatgtgtg accggttccc aatgtacggc tttgggttcc
      541 caatgtacgg gttccggttc ccaatgtacg gctttgggtt cccaatgtac gtgctatcca
      601 caggaaagag accttttcga cctttttccc ctgctagggc aatttgccct agcatctgct
      661 ccgtacatta ggaaccggcg gatgcttcgc cctcgatcag gttgcggtag cgcatgacta
      721 ggatcgggcc agcctgcccc gcctcctcct tcaaatcgta ctccggcagg tcatttgacc
      781 cgatcagctt gcgcacggtg aaacagaact tcttgaactc tccggcgctg ccactgcgtt
      841 cgtagatcgt cttgaacaac catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc
      901 ggtagagaaa acggccgatg ccgggatcga tcaaaaagta atcggggtga accgtcagca
      961 cgtccgggtt cttgccttct gtgatctcgc ggtacatcca atcagctagc tcgatctcga
     1021 tgtactccgg ccgcccggtt tcgctcttta cgatcttgta gcggctaatc aaggcttcac
     1081 cctcggatac cgtcaccagg cggccgttct tggccttctt cgtacgctgc atggcaacgt
     1141 gcgtggtgtt taaccgaatg caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg
     1201 ctcgccggca gaacttgagt acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt
     1261 ctcccttccc ttcccggtat cggttcatgg attcggttag atgggaaacc gccatcagta
     1321 ccaggtcgta atcccacaca ctggccatgc cggccggccc tgcggaaacc tctacgtgcc
     1381 cgtctggaag ctcgtagcgg atcacctcgc cagctcgtcg gtcacgcttc gacagacgga
     1441 aaacggccac gtccatgatg ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa
     1501 cgaaaaaatc tggttgctcg tcgcccttgg gcggcttcct aatcgacggc gcaccggctg
     1561 ccggcggttg ccgggattct ttgcggattc gatcagcggc cgcttgccac gattcaccgg
     1621 ggcgtgcttc tgcctcgatg cgttgccgct gggcggcctg cgcggccttc aacttctcca
     1681 ccaggtcatc acccagcgcc gcgccgattt gtaccgggcc ggatggtttg cgaccgctca
     1741 cgccgattcc tcgggcttgg gggttccagt gccattgcag ggccggcagg caacccagcc
     1801 gcttacgcct ggccaaccgc ccgttcctcc acacatgggg cattccacgg cgtcggtgcc
     1861 tggttgttct tgattttcca tgccgcctcc tttagccgct aaaattcatc tactcattta
     1921 ttcatttgct catttactct ggtagctgcg cgatgtattc agatagcagc tcggtaatgg
     1981 tcttgccttg gcgtaccgcg tacatcttca gcttggtgtg atcctccgcc ggcaactgaa
     2041 agttgacccg cttcatggct ggcgtgtctg ccaggctggc caacgttgca gccttgctgc
     2101 tgcgtgcgct cggacggccg gcacttagcg tgtttgtgct tttgctcatt ttctctttac
     2161 ctcattaact caaatgagtt ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc
     2221 agcgtcgccc tcgggttctg attcaagaac ggttgtgccg gcggcggcag tgcctgggta
     2281 gctcacgcgc tgcgtgatac gggactcaag aatgggcagc tcgtacccgg ccagcgcctc
     2341 ggcaacctca ccgccgatgc gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg
     2401 tagccttcca tccgtgacct caatgcgctg cttaaccagc tccaccaggt cggcggtggc
     2461 ccatatgtcg taagggcttg gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc
     2521 ggacacagcc aagtccgccg cctggggcgc tccgtcgatc actacgaagt cgcgccggcc
     2581 gatggccttc acgtcgcggt caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg
     2641 atcttcccgc acggccgccc aatcgcgggc actgccctgg ggatcggaat cgactaacag
     2701 aacatcggcc ccggcgagtt gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc
     2761 tgacccgcct ttctggttaa gtacagcgat aaccttcatg cgttcccctt gcgtatttgt
     2821 ttatttactc atcgcatcat atacgcagcg accgcatgac gcaagctgtt ttactcaaat
     2881 acacatcacc tttttagacg gcggcgctcg gtttcttcag cggccaagct ggccggccag
     2941 gccgccagct tggcatcaga caaaccggcc aggatttcat gcagccgcac ggttgagacg
     3001 tgcgcgggcg gctcgaacac gtacccggcc gcgatcatct ccgcctcgat ctcttcggta
     3061 atgaaaaacg gttcgtcctg gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt
     3121 cattctcggc ggccgccagg gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc
     3181 gccgcctggc ctcggtgggc gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg
     3241 agcgatgcac gccaagcagt gcagccgcct ctttcacggt gcggccttcc tggtcgatca
     3301 gctcgcgggc gtgcgcgatc tgtgccgggg tgagggtagg gcgggggcca aacttcacgc
     3361 ctcgggcctt ggcggcctcg cgcccgctcc gggtgcggtc gatgattagg gaacgctcga
     3421 actcggcaat gccggcgaac acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg
     3481 cccacggctc tgccaggcta cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt
     3541 ccagtaggtc gcgggtgctg cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc
     3601 cagggcgtag gtggtcaagc atcctggcca gctccgggcg gtcgcgcctg gtgccggtga
     3661 tcttctcgga aaacagcttg gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca
     3721 agtcctggtc gtcggtgctg acgcgggcat agcccagcag gccagcggcg gcgctcttgt
     3781 tcatggcgta atgtctccgg ttctagtcgc aagtattcta ctttatgcga ctaaaacacg
     3841 cgacaagaaa acgccaggaa aagggcaggg cggcagcctg tcgcgtaact taggacttgt
     3901 gcgacatgtc gttttcagaa gacggctgca ctgaacgtca gaagccgact gcactatagc
     3961 agcggagggg ttggatcaaa gtactttgat cccgagggga accctgtggt tggcatgcac
     4021 atacaaatgg acgaacggat aaaccttttc acgccctttt aaatatccga ttattctaat
     4081 aaacgctctt ttctcttagg tttacccgcc aatatatcct gtcaaacact gatagtttaa
     4141 actgaaggcg ggaaacgaca atctgatcca agctcaagct gctctagcat tcgccattca
     4201 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4261 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     4321 gacgttgtaa aacgacggcc agtgccaagc ttgcatgcct gcaggtcgac tctagaggat
     4381 ccccgggtac cgggcccccc ctcgaggcgc gccacgcgta gtctagctca acagagcttt
     4441 taacccaaat tggtacaata gaatacaact ttagatcata attctcaaaa gaaagagatt
     4501 ccttagctat tctatctgcc actccatttc cttctcggct tgtatgcaca agcataaaat
     4561 cctcaaactt gctaagtaga tactttatgt cttggataat tggattgaga cttgacaagc
     4621 ataactttca tgtaaccaaa gacacaagtt gctgagaatc cacctcaaaa atgatcttcc
     4681 tataattgaa tcgggataat gacagcacag cccatctaag agcctccact tctacttcca
     4741 gcacgcttct tacttttacc acagctcttg cacctaacca taacaccttc cctgtatgat
     4801 cgcgaagcac ccaccctaag ccacatttta atccttctgt tggccatgcc ccatcaaagt
     4861 tgcacttaac ccaagattgt ggtggagctt cccatgtttc tcgtctgtcc cgacggtgtt
     4921 gtggttggtg ctttccttac attctgagcc tctttccttc taatccactc atctgcatct
     4981 tcttgtgtcc ttactaatac ctcattggtt ccaaattccc tccctttaag caccagctcg
     5041 tttctgttct tccacagcct cccaagtatc caagggacta aagcctccac attcttcaga
     5101 tcaggatatt cttgtttaag atgttgaact ctatggaggt ttgtatgaac tgatgatcta
     5161 ggaccggata agttcccttc ttcatagcga acttattcaa agaatgtttt gtgtatcatt
     5221 cttgttacat tgttattaat gaaaaaatat tattggtcat tggactgaac acgagtgtta
     5281 aatatggacc aggccccaaa taagatccat tgatatatga attaaataac aagaataaat
     5341 cgagtcacca aaccacttgc cttttttaac gagacttgtt caccaacttg atacaaaagt
     5401 cattatccta tgcaaatcaa taatcataca aaaatatcca ataacactaa aaaattaaaa
     5461 gaaatggata atttcacaat atgttatacg ataaagaagt tacttttcca agaaattcac
     5521 tgattttata agcccacttg cattagataa atggcaaaaa aaaacaaaaa ggaaaagaaa
     5581 taaagcacga agaattctag aaaatacgaa atacgcttca atgcagtggg acccacggtt
     5641 caattattgc caattttcag ctccaccgta tatttaaaaa ataaaacgat aatgctaaaa
     5701 aaatataaat cgtaacgatc gttaaatctc aacggctgga tcttatgacg accgttagaa
     5761 attgtggttg tcgacgagtc agtaataaac ggcgtcaaag tggttgcagc cggcacacac
     5821 gagtcgtgtt tatcaactca aagcacaaat acttttcctc aacctaaaaa taaggcaatt
     5881 agccaaaaac aactttgcgt gtaaacaacg ctcaatacac gtgtcatttt attattagct
     5941 attgcttcac cgccttagct ttctcgtgac ctagtcgtcc tcgtcttttc ttcttcttct
     6001 tctataaaac aatacccaaa gagctcttct tcttcacaat tcagatttca atttctcaaa
     6061 atcttaaaaa ctttctctca attctctcta ccgtgatcaa ggtaaatttc tgtgttcctt
     6121 attctctcaa aatcttcgat tttgttttcg ttcgatccca atttcgtata tgttctttgg
     6181 tttagattct gttaatctta gatcgaagac gattttctgg gtttgatcgt tagatatcat
     6241 cttaattctc gattagggtt tcatagatat catccgattt gttcaaataa tttgagtttt
     6301 gtcgaataat tactcttcga tttgtgattt ctatctagat ctggtgttag tttctagttt
     6361 gtgcgatcga atttgtcgat taatctgagt ttttctgatt aacagacgcg tccatggttg
     6421 ggtaccaaca aatggctggt ggacttaacg atatcttcga agctcagaag attgaatggc
     6481 atgaggatac tggtggatct agcatccccg gacttgaggt tcttttccaa ggacctctcg
     6541 agccttataa atgccctgag tgtggaaaat ctttctctca atctggagat cttaggaggc
     6601 accaaaggac ccataccggt gaaaaacctt ataaatgccc tgagtgtgga aaatcattca
     6661 gtaggagtga tcatcttacc acccatcaga ggacccatac tggtgaaaaa ccttataaat
     6721 gccctgagtg tggaaaatca ttctcacaat cttcatctct tgttaggcat cagaggactc
     6781 ataccggaga aaaaccttat aaatgccctg agtgtggaaa atcattctca acatctggaa
     6841 accttgttag gcaccaaagg acccatactg gtgaaaaacc ttataaatgc cctgagtgtg
     6901 gaaaatcatt ctctcagaga gcaaacctta gggctcatca aaggacacac actggagaaa
     6961 aaccttataa atgccctgag tgtggaaaat ctttctctag gagtgataag ctcaccgagc
     7021 atcaaaggac tcacaccgga ctcgaggact acaaagacga tgacgacaaa gactacaaag
     7081 acgatgacga caaagactac aaagacgatg acgacaaaaa gcttgatatc gccatggggg
     7141 gcgcgccaag ctatcaaaca agtttgtaca aaaaagcagg ctccgcggcc gcccccttca
     7201 ccctagaccc tttgcctgct gctgctgccg ctgtgagcaa aggcgaagag ctgttcaccg
     7261 gcgtggtgcc catcctggtg gagctggacg gcgacgtgaa cggccacaag ttcagcgtga
     7321 gcggcgaggg cgagggcgac gccacctacg gcaagctgac cctgaagctg ctgtgcacca
     7381 ccggcaagct gcccgtgccc tggcccaccc tggtgaccac cctgggctac ggcgtgcagt
     7441 gcttcgcccg gtaccccgac cacatgaagc agcacgactt cttcaagagc gccatgcccg
     7501 agggctacgt gcaggagcgg accatcttct tcaaggacga cggcaactac aagacccggg
     7561 ccgaggtgaa gttcgagggc gacaccctgg tgaaccggat cgagctgaag ggcatcgact
     7621 tcaaggagga cggcaacatc ctgggccaca agctggagta caactacaac agccacaacg
     7681 tgtacatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccggcaca
     7741 acatcgagga cggcggcgtg cagctggccg accactacca gcagaacacc cccatcggcg
     7801 acggccccgt gctgctgccc gacaaccact acctgagcta ccagagcgcc ctgttcaagg
     7861 accccaacga gaagcgggac cacatggtgc tgctggagtt cctgaccgcc gccggcatca
     7921 ccgagggcat gaacgagctc tataattaga agggtgggcg cgccgaccca gctttcttgt
     7981 acaaagtggt tcgataattc ttaattaact agttctagag cggccgccac cgcggtggag
     8041 ctcctaggtg agtctagaga gttaattaag acccgggact agtccctaga gtcctgcttt
     8101 aatgagatat gcgagacgcc tatgatcgca tgatatttgc tttcaattct gttgtgcacg
     8161 ttgtaaaaaa cctgagcatg tgtagctcag atccttaccg ccggtttcgg ttcattctaa
     8221 tgaatatatc acccgttact atcgtatttt tatgaataat attctccgtt caatttactg
     8281 attgtaccct actacttata tgtacaatat taaaatgaaa acaatatatt gtgctgaata
     8341 ggtttatagc gacatctatg atagagcgcc acaataacaa acaattgcgt tttattatta
     8401 caaatccaat tttaaaaaaa gcggcagaac cggtcaaacc taaaagactg attacataaa
     8461 tcttattcaa atttcaaaag tgccccaggg gctagtatct acgacacacc gagcggcgaa
     8521 ctaataacgc tcactgaagg gaactccggt tccccgccgg cgcgcatggg tgagattcct
     8581 tgaagttgag tattggccgt ccgctctacc gaaagttacg ggcaccattc aacccggtcc
     8641 agcacggcgg ccgggtaacc gacttgctgc cccgagaatt atgcagcatt tttttggtgt
     8701 atgtgggccc caaatgaagt gcaggtcaaa ccttgacagt gacgacaaat cgttgggcgg
     8761 gtccagggcg aattttgcga cgagctcgaa ttcgtaatca tgtcatagct gtttcctgtg
     8821 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
     8881 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
     8941 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga
     9001 ggcggtttgc gtattggcta gagcagcttg ccaacatggt ggagcacgac actctcgtct
     9061 actccaagaa tatcaaagat acagtctcag aagaccaaag ggctattgag acttttcaac
     9121 aaagggtaat atcgggaaac ctcctcggat tccattgccc agctatctgt cacttcatca
     9181 aaaggacagt agaaaaggaa ggtggcacct acaaatgcca tcattgcgat aaaggaaagg
     9241 ctatcgttca agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga
     9301 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgaac
     9361 atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac
     9421 caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat
     9481 tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa
     9541 tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc
     9601 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     9661 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     9721 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc
     9781 tgaaatcacc agtctctctc tacaaatcta tctctctcga gtctaccatg agcccagaac
     9841 gacgcccggc cgacatccgc cgtgccaccg aggcggacat gccggcggtc tgcaccatcg
     9901 tcaaccacta catcgagaca agcacggtca acttccgtac cgagccgcag gaaccgcagg
     9961 agtggacgga cgacctcgtc cgtctgcggg agcgctatcc ctggctcgtc gccgaggtgg
    10021 acggcgaggt cgccggcatc gcctacgcgg gcccctggaa ggcacgcaac gcctacgact
    10081 ggacggccga gtcgaccgtg tacgtctccc cccgccacca gcggacggga ctgggctcca
    10141 cgctctacac ccacctgctg aagtccctgg aggcacaggg cttcaagagc gtggtcgctg
    10201 tcatcgggct gcccaacgac ccgagcgtgc gcatgcacga ggcgctcgga tatgcccccc
    10261 gcggcatgct gcgggcggcc ggcttcaagc acgggaactg gcatgacgtg ggtttctggc
    10321 agctggactt cagcctgccg gtaccgcccc gtccggtcct gcccgtcacc gagatttgac
    10381 tcgagtttct ccataataat gtgtgagtag ttcccagata agggaattag ggttcctata
    10441 gggtttcgct catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa
    10501 tacttctatc aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc
    10561 ccccgaatta attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt
    10621 tgtctaagcg tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc
    10681 cccgaccggc agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac
    10741 ggcgtcagcg ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc
    10801 ggaagaacgg caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat
    10861 gttgattgta acgatgacag agcgttgctg cctgtgatca ccgcggtttc aaaatcggct
    10921 ccgtcgatac tatgttatac gccaactttg aaaacaactt tgaaaaagct gttttctggt
    10981 atttaaggtt ttagaatgca aggaacagtg aattggagtt cgtcttgtta taattagctt
    11041 cttggggtat ctttaaatac tgtagaaaag aggaaggaaa taataaatgg ctaaaatgag
    11101 aatatcaccg gaattgaaaa aactgatcga aaaataccgc tgcgtaaaag atacggaagg
    11161 aatgtctcct gctaaggtat ataagctggt gggagaaaat gaaaacctat atttaaaaat
    11221 gacggacagc cggtataaag ggaccaccta tgatgtggaa cgggaaaagg acatgatgct
    11281 atggctggaa ggaaagctgc ctgttccaaa ggtcctgcac tttgaacggc atgatggctg
    11341 gagcaatctg ctcatgagtg aggccgatgg cgtcctttgc tcggaagagt atgaagatga
    11401 acaaagccct gaaaagatta tcgagctgta tgcggagtgc atcaggctct ttcactccat
    11461 cgacatatcg gattgtccct atacgaatag cttagacagc cgcttagccg aattggatta
    11521 cttactgaat aacgatctgg ccgatgtgga ttgcgaaaac tgggaagaag acactccatt
    11581 taaagatccg cgcgagctgt atgatttttt aaagacggaa aagcccgaag aggaacttgt
    11641 cttttcccac ggcgacctgg gagacagcaa catctttgtg aaagatggca aagtaagtgg
    11701 ctttattgat cttgggagaa gcggcagggc ggacaagtgg tatgacattg ccttctgcgt
    11761 ccggtcgatc agggaggata tcggggaaga acagtatgtc gagctatttt ttgacttact
    11821 ggggatcaag cctgattggg agaaaataaa atattatatt ttactggatg aattgtttta
    11881 gtacctagaa tgcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    11941 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    12001 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    12061 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct
    12121 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    12181 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    12241 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    12301 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    12361 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    12421 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    12481 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    12541 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    12601 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    12661 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    12721 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
    12781 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
    12841 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
    12901 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
    12961 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
    13021 cagggtgcct tgatgtgggc gccttatttg ccgactacct tggtgatctc gcctttcacg
    13081 tagtggacaa attcttccaa ctgatctgcg cgcgaggcca agcgatcttc ttcttgtcca
    13141 agataagcct gtctagcttc aagtatgacg ggctgatact gggccggcag gcgctccatt
    13201 gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct gtaccaaatg
    13261 cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg cgagttccat
    13321 agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg atcaaagagt
    13381 tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt cagcaagata
    13441 gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc attgcgctgc
    13501 cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat gtcgtcgtgc
    13561 acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga agccgaagtt
    13621 tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac ggtcaccgta
    13681 accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga gccgtacaaa
    13741 tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac ctctgatagt
    13801 tgagtcgata cttcggcgat caccgcttcc ctcat
//
