LOCUS       sequence-205529-        3779 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Synthetic sequence for single-stranded DNA production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Veneziano R, Shepherd TR, Ratanalert S, Bellou L, Tao C, Bathe M
  TITLE     In vitro synthesis of gene-length single-stranded DNA.
  JOURNAL   Sci Rep. 2018 Apr 25;8(1):6548. doi: 10.1038/s41598-018-24677-5.
  PUBMED    29695837
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-04-25"; volume: "8(1):6548. doi"; pages: " 
            10.1038/s41598-018-24677-5"
COMMENT     Sequence Label: pUC19-Cv3
FEATURES             Location/Qualifiers
     source          1..3779
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1558..1574)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1558..1574)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1571..1593)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1582..1598
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1606..1612,1613..1630,1631..1636))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1651..1672
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1789..1806)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1960..2548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2040..2059)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2719..3510,3511..3579))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3342..3361
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3580..3684)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3752..3770
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat
      421 cctctagagt cgacctgcag gtcgtcgtcc cctcaaactc ttgggtggag aggctattcg
      481 tttaaggtca catcgcatgt aatttactta ttctctgttg ttgagccacc cgggcgccag
      541 attttgttta aagctttgtc tcttagtttg tatagacaga ttcagagtgc aaggtttcgt
      601 tcgctcgtac ctggttttcc ctggttcttc acagatagga tttgactttc tacaacactt
      661 atgcggcttc ctacccgttt gaaggccgat acaggtgctg cgcaaaatgc gggcgaacat
      721 agagtatcaa aacaacgcct tctaatctag gaatataggg aagatacgta tttgctacca
      781 tgctttcttg ggtcattaac gaccaacctc ttttctttta aagtaggatt gcacaatgaa
      841 tgaatacacg tggtccgata actgaccaag taacatggtt atcactcgat gtccgccaga
      901 cgtgtgcaaa ccaacccggg agttacgtca ctaatccttc gctacgtcgt gaagatattt
      961 acttgtgaat atcgagggta ataagataat agactgtgac tagtattgcc agactgtcgc
     1021 tacctgcaac acataactat cctgaggtta ctgcatagta ctgattacac ccgagtcaaa
     1081 atttctaact tctaacatgt acctagtaac cagctcaata attatgtcag aatatagctc
     1141 tgggaaccct cggacaatta tgatacacgg tattaatatc ttgcttgcgt tagccacttc
     1201 tcatctttgg ataccgattc tattttgcat agcagttcct tttacacata taagaatttc
     1261 gccataggta tgacctaccc cagatcgtcg attatctgct ggaaaattta tttaacacta
     1321 tgtttctctc cagatgtgag tatacacgat aaataatacc tgggtaccgg ttggtgttat
     1381 taccttgttt ctaagtgctt aatcggcgct tagtgataag gttgtactag tcgacgcgtg
     1441 gccgccaatt attcttgtca taatttgact ttgttctata tgactatgat ctcctgtcat
     1501 ctcacctatt gatgccacct tttcagcctg caggcatgca agcttggcgt aatcatggtc
     1561 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     1621 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     1681 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     1741 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     1801 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     1861 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     1921 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     1981 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     2041 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     2101 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     2161 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     2221 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     2281 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     2341 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     2401 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     2461 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     2521 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     2581 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     2641 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     2701 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     2761 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     2821 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     2881 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     2941 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     3001 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     3061 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     3121 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     3181 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     3241 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     3301 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     3361 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     3421 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     3481 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     3541 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     3601 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     3661 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     3721 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc
//
