LOCUS       sequence-205532-       10710 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10710)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-317
FEATURES             Location/Qualifiers
     source          1..10710
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     15..34
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     118..137
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        118..136
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    137..161
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             215..232
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3780..3869
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3882..3971
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5762..5780)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5776..5823
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5860..6315
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5947..5966)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6157..6178
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6407..7222)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6407..7222)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7130..7149
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7214..7233
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7344..7932
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7833..7852
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8086..8103
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8118..8257
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8183..8202)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8343..8365
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8359..8550)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(9359..10441)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     10403..10422
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(10442..10519)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg
       61 gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaattaa
      121 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgc
      181 cttaacttta agaaggagat ataccatggg cagccaccac caccaccacc acagcggaat
      241 gcagatcttt gttaagactc tcaccggaaa gacaatcacc ctagaggtgg aaagctccga
      301 caccatcgac aacgttaagg ccaagatcca ggataaggag ggcattcctc cggatcagca
      361 gaggcttatt ttcgccggca agcagctaga ggatggccgt acgttggctg attacaatat
      421 ccagaaggaa tccaccctcc acttggtcct caggctccgt ggtggttaag cttgcaatta
      481 actttaagaa ggagatatac aatgcttcac aagcgagcta gtgaagctaa cgataagaac
      541 gataatacca tcatcggttc cgatttagca tcatctaaga aacgccgtat cgatttcact
      601 gaatcttcgt ctgataagtc atcttcgatt ttagcttctg gtagtagcag gggttttcac
      661 ggcgatagcg tcgttcagca aatcgacatg gcttttggta attcgaaccg tcaggagatt
      721 gatgaagatc tgcacagtcg ccagctcgcc gtctatggtc gtgagactat gaggcgtctc
      781 tttgcttcca atgttctcat ctcggggatg cacggtcttg gtgccgagat tgccaagaat
      841 cttatacttg ctggtgtgaa gtctgtgacc ttgcatgatg aaagagttgt agagctatgg
      901 gacttatcaa gcaactttgt tttctcggag gatgatgttg gcaagaatag agcagatgct
      961 tctgttcaga agttacagga tcttaacaat gctgtggttg tctctagctt gaccaaaagc
     1021 ttaaacaaag aggatctttc tggcttccag gttgttgtat tctctgacat aagcatggaa
     1081 agagcaatcg aatttgatga ctattgtcac agccatcagc ctcctatcgc ttttgtcaag
     1141 gctgatgtca ggggtctttt cggctctgtc ttttgtgatt ttgggcctga atttgcggtt
     1201 cttgatgttg atggagagga accccataca ggcatcattg cgtctatctc taatgagaac
     1261 caggcattta tttcctgtgt cgacgatgag aggcttgagt ttgaagatgg tgacctagtt
     1321 gttttctcag aagttgaggg tatgacagaa ttgaatgatg ggaaaccaag gaagataaaa
     1381 agtacacggc catattcatt cacccttgat gaggacacta ctaactatgg aacatatgtg
     1441 aagggtggta ttgtcactca agtgaaacag ccaaagttac tgaatttcaa gccattgagg
     1501 gaagcgctta aggatccagg ggattttcta tttagtgatt tctctaagtt tgatcggcct
     1561 ccactccttc acttagcatt ccaggcactt gatcatttta aagctgaagc tggcagattt
     1621 cctgttgctg gctcagaaga ggacgctcag aagcttatat cgattgccac agccatcaat
     1681 accgggcagg gtgatttaaa ggtggagaat gttgatcaga agcttctaag acacttttcc
     1741 tttggagcca aggctgtcct gaatcctatg gctgcaatgt ttggcggtat tgttggacag
     1801 gaggttgtta aagcttgctc tggaaaattt catcctctct tccagttttt ctactttgat
     1861 tcagtggagt cactcccctc tgaacctgtg gactcgagtg actttgcacc aagaaacagc
     1921 cggtatgatg cccaaatatc tgtatttggg gccaagttcc agaagaaact tgaagatgct
     1981 aaagttttca cagtagggtc tggtgctctt ggctgtgagt tcttgaaaaa tttggctctg
     2041 atgggggttt cttgtggaag ccaagggaag ctgacagtga cagatgatga tatcattgag
     2101 aagagtaacc tcagtcgtca atttctgttc cgtgactgga acattggaca ggctaaatcc
     2161 acagttgctg cttcggctgc tgctgttata aatcctcgct tcaacattga ggccctgcaa
     2221 aaccgtgttg gcgcggagac tgagaatgta tttgacgatg ccttctggga gaacttaact
     2281 gttgtcgtca atgcactgga taatgttaat gcgaggctct atgttgattc gaggtgcttg
     2341 tatttccaga agcctctcct tgagtctgga actcttggta caaagtgcaa cacacagtcg
     2401 gttatcccac atctaactga aaattacggt gcctcaaggg acccaccaga gaaacaggcc
     2461 cccatgtgta cagtgcattc cttcccgcat aacattgatc actgtttaac ttgggctcgc
     2521 tctgagtttg agggtctgct ggagaaaact cccgctgaag tgaatgcata tctttctagc
     2581 ccagttgagt acactaactc aatgatgagt gctggtgatg ctcaggcgag ggacacattg
     2641 gagaggattg ttgagtgcct tgaaaaggag aagtgtgaaa ccttccaaga ctgcttaacc
     2701 tgggcacgac tgaggtttga ggattacttt gtgaaccgtg tcaagcaatt gatatacaca
     2761 tttcctgaag atgctgcgac tagtaccgga gctccattct ggtctgctcc aaaaagattc
     2821 cctcgtccac tccagtactc gtcgtccgac ccaagtctcc ttaactttat cacagcgact
     2881 gctattttac gagcagagac atttgggatc ccgatacctg agtggactaa gaacccaaag
     2941 gaagcagcag aagctgttga cagagtgata gtcccagact ttgagccaag gcaagatgca
     3001 aagattgtga ctgatgagaa agctaccact ttaaccactg cttcggtgga cgacgctgca
     3061 gtcatcgacg atctcattgc taagatcgac cagtgtaggc ataacttgtc gccagacttc
     3121 aggatgaaac ctattcagtt cgaaaaggat gatgatacaa actatcacat ggatgtgata
     3181 gcaggactag ccaacatgag ggcgagaaat tacagcatcc ctgaagtgga caagctgaaa
     3241 gcaaagttca tagcagggag aatcatccca gccattgcga cttcaacagc catggctact
     3301 gggctggtct gcctcgagct gtacaaggtc cttgatggag gacataaagt ggaagcttac
     3361 agaaacacat ttgccaacct ggcacttcca ctattctcca tggctgaacc acttccacca
     3421 aaggtggtga agcaccgtga catggcttgg acagtttggg acagatgggt tctcaaggga
     3481 aacccaactc ttcgtgaggt gttgcagtgg ctggaggaca aagggcttag cgcatacagc
     3541 atctcttgtg gaagctgtct actgttcaac agtatgttca caaggcacaa ggagaggatg
     3601 gacaagaagg ttgtggatct cgctcgggat gttgcgaaag tcgagttacc gccttatcgc
     3661 aaccatcttg atgtagtggt ggcttgtgag gatgaagatg acaatgacgt cgatattcct
     3721 ctcgtctcta tctacttcag gtgagcttac tattaacttt aagaaggaga tataccatgt
     3781 acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat
     3841 tgtatccata tgacgttcca gattacgcca ctagagctgc ttacccatac gatgttcctg
     3901 actatgcggg ctatccctat gacgtcccgg actatgcagg attgtatcca tatgacgttc
     3961 cagattacgc aatggagcct gacgtggtgg agatccctcc tcctcctctg atcgcttcgg
     4021 gttctagaac tcgtaaaccc agaaaggctg ttcctgaagt gattgatgtg gaaagttatg
     4081 aattccgcaa tgttggtgtt gttaaggata acaatgttgt tgataagaag aacaaaggga
     4141 aggcaataca agttgactcc ttttctttca acaacgttca atctcatcat catggatcgt
     4201 cgctgcttaa cttagagacg ttccaagact attatggtca taagaatatc ccttttagtg
     4261 aatttgctaa tcaaccaatt gatgttgatg actactcaat gtatcaagac gttctagatc
     4321 ctaaggatgt tccagctggt gcagaggtga cggtaccttg gggtttaaat tcgagcagta
     4381 agggaactgc gaaatcaagt ataagcatta tgagaagtca atccatgaaa ggatatggaa
     4441 cagtatctct ggcaacgact aatgttcctc aattatggga ttatactttg cctcagcaga
     4501 atcaggctat ctattcttct gtatcgtttt ctgcggttca acctcagact cctgatgtag
     4561 tgatggtcac caatcctact ccaaaccctt ttagttatga tgcttctgct agttcttctc
     4621 atcctatagc ggctgagcct atttcttcag ttcaggattc ttccaatgct aggaaactta
     4681 aggaagagtt tctgagggat tttaaaagat ttgacaccgt cgaggatttc tcagatcatc
     4741 actatgcttc aaagggtaaa tcttcaaagc agcactcgaa gaattgggtg aaaaaggttc
     4801 aagcagattg gaagattctt gagaatgatt tgccagaggc aatatctgtc agagcctgtg
     4861 aatcaagaat ggatcttttg agagctgtaa ttattggagc tgagggaact ccttaccatg
     4921 acggtctttt cttcttcgat attcagttcc cagatactta tccttcagtg ccaccgaatg
     4981 ttcattacca ttctggtggg cttagaatca acccgaatct atacaattgt gggaaagtat
     5041 gcttgagtct actcggtacc tgggcaggta gcgcgaggga gaaatggctc ccaaatgagt
     5101 cgacaatgtt acagcttctt gtctcaatcc aagcactcat cttgaatgaa aaaccatact
     5161 ttaacgaacc tggctacgta cagagcgcgg ggactgcatc aggcgagtcc aagtctaaag
     5221 tttacagtga gaacgtattt ctattgtcgt taaagactat ggtttacagc ataagaaggc
     5281 caccccagca ttttgaagag tatgttcaaa accattactt tgtccgctct cacgacattg
     5341 tgaaagcatg caatgcttat aaagctggag ctccgcttgg atcgatggtt aaaggcggag
     5401 tccaagacct tgaagaagct agacagagcg gctccaagaa gtttaagacc gatgtagcta
     5461 gctttatgca gacagtggtt gatgaattcg tcaaactagg agtcaaggag cttgcagaaa
     5521 aaccagaacc gcctatgagc aatgctaaca cagagaatca gagcaagaaa aaaacccgta
     5581 agagaagcag atcatcgagg tgagctttta cgcttttaag ccagccccga cacccgccaa
     5641 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     5701 tgaccgggat gagatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     5761 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     5821 ttgctgaaag gaggaactat atccggattg gcgaatggga cgcgccctgt agcggcgcat
     5881 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5941 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6001 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6061 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6121 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     6181 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     6241 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6301 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     6361 atttttctaa atacattcaa atatgtatcc gctcatgaat taattcttag aaaaactcat
     6421 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa
     6481 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat
     6541 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct
     6601 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga
     6661 atggcaaaag tttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt
     6721 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagtc
     6781 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca
     6841 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct
     6901 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga
     6961 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct
     7021 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat
     7081 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc
     7141 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggccta gagcaagacg
     7201 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt
     7261 ttattgttca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     7321 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     7381 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     7441 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     7501 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     7561 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     7621 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     7681 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     7741 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     7801 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     7861 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     7921 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     7981 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     8041 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     8101 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     8161 caccgcaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata
     8221 cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc
     8281 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccga
     8341 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct
     8401 gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc
     8461 gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat
     8521 gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt
     8581 catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga
     8641 tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg
     8701 ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt
     8761 tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc
     8821 tgacttccgc gtttccagac tttacgaaac acggaaaccc aagaccattc atgttgttgc
     8881 tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc
     8941 attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac
     9001 gatcatgcgc acccgtgggg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg
     9061 tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc
     9121 aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca
     9181 gagcgctgcc ggcacctgtc ctacgagttg catgataaag aacacagtca taagtgcggc
     9241 gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     9301 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc
     9361 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     9421 cgcggggaga ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaca
     9481 cgggcaacag ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca
     9541 cgctggtttg ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac
     9601 atgagctgtc ctcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc
     9661 cggactcggt aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg
     9721 cagtgggaac gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac
     9781 tccagtcgcc ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc
     9841 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
     9901 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtcc tcatgggaga
     9961 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
    10021 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
    10081 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
    10141 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
    10201 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
    10261 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
    10321 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
    10381 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
    10441 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
    10501 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
    10561 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
    10621 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
    10681 caagcgctca tgagcccgaa gtggcgagcc
//
