LOCUS       sequence-205533-        9657 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-318
FEATURES             Location/Qualifiers
     source          1..9657
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     922..941
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(961..1038)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1244..1263
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1347..1366
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1347..1365
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1366..1390
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1444..1461
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5009..5098
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5111..5200
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5938..5956)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5952..5999
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6036..6491
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6123..6142)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6333..6354
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6583..7398)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6583..7398)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7306..7325
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7390..7409
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7520..8108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8009..8028
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8262..8279
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8294..8433
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8359..8378)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8519..8541
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8535..8726)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9535..9657,1..960))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca agcggtccac
       61 gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg ggatataaca
      121 tgagctgtcc tcggtatcgt cgtatcccac taccgagata tccgcaccaa cgcgcagccc
      181 ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc
      241 agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg acatggcact
      301 ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat atttatgcca
      361 gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca gcgcgatttg
      421 ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtcct catgggagaa
      481 aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg gaacattagt
      541 gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa tgatcagccc
      601 actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga cgccgcttcg
      661 ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt taatcgccgc
      721 gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa tcagcaacga
      781 ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct ccgccatcgc
      841 cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca ccacgcggga
      901 aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta ctggtttcac
      961 attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc gaaaggtttt
     1021 gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc tgcattagga
     1081 agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca
     1141 aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac
     1201 aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat
     1261 aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga
     1321 ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga
     1381 taacaattcc cctctagaaa taattttgcc ttaactttaa gaaggagata taccatgggc
     1441 agccaccacc accaccacca cagcggaatg cagatctttg ttaagactct caccggaaag
     1501 acaatcaccc tagaggtgga aagctccgac accatcgaca acgttaaggc caagatccag
     1561 gataaggagg gcattcctcc ggatcagcag aggcttattt tcgccggcaa gcagctagag
     1621 gatggccgta cgttggctga ttacaatatc cagaaggaat ccaccctcca cttggtcctc
     1681 aggctccgtg gtggttaagc ttgcaattaa ctttaagaag gagatataca atgcttcaca
     1741 agcgagctag tgaagctaac gataagaacg ataataccat catcggttcc gatttagcat
     1801 catctaagaa acgccgtatc gatttcactg aatcttcgtc tgataagtca tcttcgattt
     1861 tagcttctgg tagtagcagg ggttttcacg gcgatagcgt cgttcagcaa atcgacatgg
     1921 cttttggtaa ttcgaaccgt caggagattg atgaagatct gcacagtcgc cagctcgccg
     1981 tctatggtcg tgagactatg aggcgtctct ttgcttccaa tgttctcatc tcggggatgc
     2041 acggtcttgg tgccgagatt gccaagaatc ttatacttgc tggtgtgaag tctgtgacct
     2101 tgcatgatga aagagttgta gagctatggg acttatcaag caactttgtt ttctcggagg
     2161 atgatgttgg caagaataga gcagatgctt ctgttcagaa gttacaggat cttaacaatg
     2221 ctgtggttgt ctctagcttg accaaaagct taaacaaaga ggatctttct ggcttccagg
     2281 ttgttgtatt ctctgacata agcatggaaa gagcaatcga atttgatgac tattgtcaca
     2341 gccatcagcc tcctatcgct tttgtcaagg ctgatgtcag gggtcttttc ggctctgtct
     2401 tttgtgattt tgggcctgaa tttgcggttc ttgatgttga tggagaggaa ccccatacag
     2461 gcatcattgc gtctatctct aatgagaacc aggcatttat ttcctgtgtc gacgatgaga
     2521 ggcttgagtt tgaagatggt gacctagttg ttttctcaga agttgagggt atgacagaat
     2581 tgaatgatgg gaaaccaagg aagataaaaa gtacacggcc atattcattc acccttgatg
     2641 aggacactac taactatgga acatatgtga agggtggtat tgtcactcaa gtgaaacagc
     2701 caaagttact gaatttcaag ccattgaggg aagcgcttaa ggatccaggg gattttctat
     2761 ttagtgattt ctctaagttt gatcggcctc cactccttca cttagcattc caggcacttg
     2821 atcattttaa agctgaagct ggcagatttc ctgttgctgg ctcagaagag gacgctcaga
     2881 agcttatatc gattgccaca gccatcaata ccgggcaggg tgatttaaag gtggagaatg
     2941 ttgatcagaa gcttctaaga cacttttcct ttggagccaa ggctgtcctg aatcctatgg
     3001 ctgcaatgtt tggcggtatt gttggacagg aggttgttaa agcttgctct ggaaaatttc
     3061 atcctctctt ccagtttttc tactttgatt cagtggagtc actcccctct gaacctgtgg
     3121 actcgagtga ctttgcacca agaaacagcc ggtatgatgc ccaaatatct gtatttgggg
     3181 ccaagttcca gaagaaactt gaagatgcta aagttttcac agtagggtct ggtgctcttg
     3241 gctgtgagtt cttgaaaaat ttggctctga tgggggtttc ttgtggaagc caagggaagc
     3301 tgacagtgac agatgatgat atcattgaga agagtaacct cagtcgtcaa tttctgttcc
     3361 gtgactggaa cattggacag gctaaatcca cagttgctgc ttcggctgct gctgttataa
     3421 atcctcgctt caacattgag gccctgcaaa accgtgttgg cgcggagact gagaatgtat
     3481 ttgacgatgc cttctgggag aacttaactg ttgtcgtcaa tgcactggat aatgttaatg
     3541 cgaggctcta tgttgattcg aggtgcttgt atttccagaa gcctctcctt gagtctggaa
     3601 ctcttggtac aaagtgcaac acacagtcgg ttatcccaca tctaactgaa aattacggtg
     3661 cctcaaggga cccaccagag aaacaggccc ccatgtgtac agtgcattcc ttcccgcata
     3721 acattgatca ctgtttaact tgggctcgct ctgagtttga gggtctgctg gagaaaactc
     3781 ccgctgaagt gaatgcatat ctttctagcc cagttgagta cactaactca atgatgagtg
     3841 ctggtgatgc tcaggcgagg gacacattgg agaggattgt tgagtgcctt gaaaaggaga
     3901 agtgtgaaac cttccaagac tgcttaacct gggcacgact gaggtttgag gattactttg
     3961 tgaaccgtgt caagcaattg atatacacat ttcctgaaga tgctgcgact agtaccggag
     4021 ctccattctg gtctgctcca aaaagattcc ctcgtccact ccagtactcg tcgtccgacc
     4081 caagtctcct taactttatc acagcgactg ctattttacg agcagagaca tttgggatcc
     4141 cgatacctga gtggactaag aacccaaagg aagcagcaga agctgttgac agagtgatag
     4201 tcccagactt tgagccaagg caagatgcaa agattgtgac tgatgagaaa gctaccactt
     4261 taaccactgc ttcggtggac gacgctgcag tcatcgacga tctcattgct aagatcgacc
     4321 agtgtaggca taacttgtcg ccagacttca ggatgaaacc tattcagttc gaaaaggatg
     4381 atgatacaaa ctatcacatg gatgtgatag caggactagc caacatgagg gcgagaaatt
     4441 acagcatccc tgaagtggac aagctgaaag caaagttcat agcagggaga atcatcccag
     4501 ccattgcgac ttcaacagcc atggctactg ggctggtctg cctcgagctg tacaaggtcc
     4561 ttgatggagg acataaagtg gaagcttaca gaaacacatt tgccaacctg gcacttccac
     4621 tattctccat ggctgaacca cttccaccaa aggtggtgaa gcaccgtgac atggcttgga
     4681 cagtttggga cagatgggtt ctcaagggaa acccaactct tcgtgaggtg ttgcagtggc
     4741 tggaggacaa agggcttagc gcatacagca tctcttgtgg aagctgtcta ctgttcaaca
     4801 gtatgttcac aaggcacaag gagaggatgg acaagaaggt tgtggatctc gctcgggatg
     4861 ttgcgaaagt cgagttaccg ccttatcgca accatcttga tgtagtggtg gcttgtgagg
     4921 atgaagatga caatgacgtc gatattcctc tcgtctctat ctacttcagg tgagcttact
     4981 attaacttta agaaggagat ataccatgta cccatacgat gttcctgact atgcgggcta
     5041 tccctatgac gtcccggact atgcaggatt gtatccatat gacgttccag attacgccac
     5101 tagagctgct tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga
     5161 ctatgcagga ttgtatccat atgacgttcc agattacgca atgatagatt tcagtcgaat
     5221 ccagaaagag cttcaggact gcgagagaaa ccaggattct tcgggtatcc gggtctgccc
     5281 caaatccgat aacctcaccc gactcaccgg aaccattcct ggtccaatcg gtactcctta
     5341 cgaaggcggt acatttcaga tcgatatcac tatgccagat gggtatccat ttgagcctcc
     5401 aaagatgcag ttttcaacta aagtttggca tccgaatatt agtagccaaa gcggtgcaat
     5461 atgcttggat atcttgaaag accagtggag tccagctctt actctcaaga cagctcttgt
     5521 ttcaattcag gctttactct ctgcacctga gcccaaagac cctcaagatg ctgttgtagc
     5581 tgaacagtac atgaagaact atcaagtgtt tgtatcaaca gctcgttact ggactgaaac
     5641 tttcgccaag aaatcttctc tagaggaaaa agtcaagaga cttgtggaga tgggttttgg
     5701 agacgctcaa gttaggagtg caattgaatc aagtggtggt gatgagaatc tggctctgga
     5761 aaagctctgt tctgcttagg cttttacgct tttaagccag ccccgacacc cgccaacacc
     5821 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     5881 cgggatgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     5941 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     6001 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
     6061 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     6121 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     6181 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     6241 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     6301 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     6361 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     6421 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     6481 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     6541 ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag
     6601 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     6661 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     6721 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     6781 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     6841 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     6901 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa
     6961 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     7021 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     7081 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     7141 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     7201 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     7261 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     7321 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc
     7381 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     7441 tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     7501 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     7561 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     7621 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     7681 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     7741 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     7801 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     7861 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     7921 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7981 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     8041 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     8101 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     8161 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     8221 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     8281 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     8341 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     8401 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     8461 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgactcc
     8521 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     8581 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
     8641 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     8701 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
     8761 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
     8821 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
     8881 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
     8941 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
     9001 ttccgcgttt ccagacttta cgaaacacgg aaacccaaga ccattcatgt tgttgctcag
     9061 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     9121 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     9181 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
     9241 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
     9301 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     9361 gctgccggca cctgtcctac gagttgcatg ataaagaaca cagtcataag tgcggcgacg
     9421 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     9481 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     9541 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     9601 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgacac
//
