LOCUS       sequence-205535-        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-320
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     55..74
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     158..177
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        158..176
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    177..201
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             255..272
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3820..3909
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3922..4011
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4617..4635)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4631..4678
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4715..5170
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4802..4821)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5012..5033
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5262..6077)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5262..6077)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     5985..6004
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6069..6088
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6199..6787
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6688..6707
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6941..6958
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6973..7112
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7038..7057)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7198..7220
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7214..7405)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8214..9296)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9258..9277
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9297..9374)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat
       61 gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg
      121 tccggcgtag aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa
      181 ttgtgagcgg ataacaattc ccctctagaa ataattttgc cttaacttta agaaggagat
      241 ataccatggg cagccaccac caccaccacc acagcggaat gcagatcttt gttaagactc
      301 tcaccggaaa gacaatcacc ctagaggtgg aaagctccga caccatcgac aacgttaagg
      361 ccaagatcca ggataaggag ggcattcctc cggatcagca gaggcttatt ttcgccggca
      421 agcagctaga ggatggccgt acgttggctg attacaatat ccagaaggaa tccaccctcc
      481 acttggtcct caggctccgt ggtggttaag cttgcaatta actttaagaa ggagatatac
      541 aatgcttcac aagcgagcta gtgaagctaa cgataagaac gataatacca tcatcggttc
      601 cgatttagca tcatctaaga aacgccgtat cgatttcact gaatcttcgt ctgataagtc
      661 atcttcgatt ttagcttctg gtagtagcag gggttttcac ggcgatagcg tcgttcagca
      721 aatcgacatg gcttttggta attcgaaccg tcaggagatt gatgaagatc tgcacagtcg
      781 ccagctcgcc gtctatggtc gtgagactat gaggcgtctc tttgcttcca atgttctcat
      841 ctcggggatg cacggtcttg gtgccgagat tgccaagaat cttatacttg ctggtgtgaa
      901 gtctgtgacc ttgcatgatg aaagagttgt agagctatgg gacttatcaa gcaactttgt
      961 tttctcggag gatgatgttg gcaagaatag agcagatgct tctgttcaga agttacagga
     1021 tcttaacaat gctgtggttg tctctagctt gaccaaaagc ttaaacaaag aggatctttc
     1081 tggcttccag gttgttgtat tctctgacat aagcatggaa agagcaatcg aatttgatga
     1141 ctattgtcac agccatcagc ctcctatcgc ttttgtcaag gctgatgtca ggggtctttt
     1201 cggctctgtc ttttgtgatt ttgggcctga atttgcggtt cttgatgttg atggagagga
     1261 accccataca ggcatcattg cgtctatctc taatgagaac caggcattta tttcctgtgt
     1321 cgacgatgag aggcttgagt ttgaagatgg tgacctagtt gttttctcag aagttgaggg
     1381 tatgacagaa ttgaatgatg ggaaaccaag gaagataaaa agtacacggc catattcatt
     1441 cacccttgat gaggacacta ctaactatgg aacatatgtg aagggtggta ttgtcactca
     1501 agtgaaacag ccaaagttac tgaatttcaa gccattgagg gaagcgctta aggatccagg
     1561 ggattttcta tttagtgatt tctctaagtt tgatcggcct ccactccttc acttagcatt
     1621 ccaggcactt gatcatttta aagctgaagc tggcagattt cctgttgctg gctcagaaga
     1681 ggacgctcag aagcttatat cgattgccac agccatcaat accgggcagg gtgatttaaa
     1741 ggtggagaat gttgatcaga agcttctaag acacttttcc tttggagcca aggctgtcct
     1801 gaatcctatg gctgcaatgt ttggcggtat tgttggacag gaggttgtta aagcttgctc
     1861 tggaaaattt catcctctct tccagttttt ctactttgat tcagtggagt cactcccctc
     1921 tgaacctgtg gactcgagtg actttgcacc aagaaacagc cggtatgatg cccaaatatc
     1981 tgtatttggg gccaagttcc agaagaaact tgaagatgct aaagttttca cagtagggtc
     2041 tggtgctctt ggctgtgagt tcttgaaaaa tttggctctg atgggggttt cttgtggaag
     2101 ccaagggaag ctgacagtga cagatgatga tatcattgag aagagtaacc tcagtcgtca
     2161 atttctgttc cgtgactgga acattggaca ggctaaatcc acagttgctg cttcggctgc
     2221 tgctgttata aatcctcgct tcaacattga ggccctgcaa aaccgtgttg gcgcggagac
     2281 tgagaatgta tttgacgatg ccttctggga gaacttaact gttgtcgtca atgcactgga
     2341 taatgttaat gcgaggctct atgttgattc gaggtgcttg tatttccaga agcctctcct
     2401 tgagtctgga actcttggta caaagtgcaa cacacagtcg gttatcccac atctaactga
     2461 aaattacggt gcctcaaggg acccaccaga gaaacaggcc cccatgtgta cagtgcattc
     2521 cttcccgcat aacattgatc actgtttaac ttgggctcgc tctgagtttg agggtctgct
     2581 ggagaaaact cccgctgaag tgaatgcata tctttctagc ccagttgagt acactaactc
     2641 aatgatgagt gctggtgatg ctcaggcgag ggacacattg gagaggattg ttgagtgcct
     2701 tgaaaaggag aagtgtgaaa ccttccaaga ctgcttaacc tgggcacgac tgaggtttga
     2761 ggattacttt gtgaaccgtg tcaagcaatt gatatacaca tttcctgaag atgctgcgac
     2821 tagtaccgga gctccattct ggtctgctcc aaaaagattc cctcgtccac tccagtactc
     2881 gtcgtccgac ccaagtctcc ttaactttat cacagcgact gctattttac gagcagagac
     2941 atttgggatc ccgatacctg agtggactaa gaacccaaag gaagcagcag aagctgttga
     3001 cagagtgata gtcccagact ttgagccaag gcaagatgca aagattgtga ctgatgagaa
     3061 agctaccact ttaaccactg cttcggtgga cgacgctgca gtcatcgacg atctcattgc
     3121 taagatcgac cagtgtaggc ataacttgtc gccagacttc aggatgaaac ctattcagtt
     3181 cgaaaaggat gatgatacaa actatcacat ggatgtgata gcaggactag ccaacatgag
     3241 ggcgagaaat tacagcatcc ctgaagtgga caagctgaaa gcaaagttca tagcagggag
     3301 aatcatccca gccattgcga cttcaacagc catggctact gggctggtct gcctcgagct
     3361 gtacaaggtc cttgatggag gacataaagt ggaagcttac agaaacacat ttgccaacct
     3421 ggcacttcca ctattctcca tggctgaacc acttccacca aaggtggtga agcaccgtga
     3481 catggcttgg acagtttggg acagatgggt tctcaaggga aacccaactc ttcgtgaggt
     3541 gttgcagtgg ctggaggaca aagggcttag cgcatacagc atctcttgtg gaagctgtct
     3601 actgttcaac agtatgttca caaggcacaa ggagaggatg gacaagaagg ttgtggatct
     3661 cgctcgggat gttgcgaaag tcgagttacc gccttatcgc aaccatcttg atgtagtggt
     3721 ggcttgtgag gatgaagatg acaatgacgt cgatattcct ctcgtctcta tctacttcag
     3781 gtgagcttac tattaacttt aagaaggaga tataccatgt acccatacga tgttcctgac
     3841 tatgcgggct atccctatga cgtcccggac tatgcaggat tgtatccata tgacgttcca
     3901 gattacgcca ctagagctgc ttacccatac gatgttcctg actatgcggg ctatccctat
     3961 gacgtcccgg actatgcagg attgtatcca tatgacgttc cagattacgc aatggcaacg
     4021 agacggatat tgaaagaatt gaaggagttg caaagggacc ctcctgtatc atgcagtgca
     4081 ggtccaacag gagaagatat gttccactgg caagctacta taatgggtcc gaatgaaagt
     4141 ccttactccg gaggtgtttt ccttgtcaat attcatttcc ctcctgatta tccttttaaa
     4201 cctcccaagg ttgtattcag aaccaaagtg tttcacccaa acatcaacag taatggaaac
     4261 atatgtttgg acattctcaa agaccaatgg agccctgccc ttaccatttc taaggtgctt
     4321 ctctctatat gctctcttct aacagatcca aaccctgacg accctctggt gccagagata
     4381 gctcacatat acaaaactga caagaccaaa tatgaagcca tggctcgaag ctggacccag
     4441 aagtatgcct tgttttaagc ttttacgctt ttaagccagc cccgacaccc gccaacaccc
     4501 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     4561 gggatgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     4621 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     4681 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     4741 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     4801 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     4861 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     4921 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     4981 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5041 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5101 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5161 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5221 tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc
     5281 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     5341 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     5401 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     5461 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     5521 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     5581 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat
     5641 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     5701 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     5761 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     5821 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     5881 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     5941 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     6001 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     6061 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     6121 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6181 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6241 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6301 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     6361 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6421 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6481 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6541 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6601 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6661 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6721 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6781 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6841 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6901 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6961 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     7021 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     7081 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     7141 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgactccg
     7201 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     7261 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     7321 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     7381 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     7441 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     7501 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     7561 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     7621 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     7681 tccgcgtttc cagactttac gaaacacgga aacccaagac cattcatgtt gttgctcagg
     7741 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     7801 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     7861 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     7921 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     7981 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     8041 ctgccggcac ctgtcctacg agttgcatga taaagaacac agtcataagt gcggcgacga
     8101 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     8161 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     8221 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
     8281 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgacacgggc
     8341 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
     8401 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
     8461 ctgtcctcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
     8521 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
     8581 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
     8641 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
     8701 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
     8761 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcctcatg ggagaaaata
     8821 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
     8881 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
     8941 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
     9001 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
     9061 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
     9121 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
     9181 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
     9241 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
     9301 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
     9361 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca
     9421 gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga
     9481 gatggcgccc aacagtcccc cggccacggg gcctgccacc atacc
//
