LOCUS       sequence-205537-        6354 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses E. coli HPII catalase with TAG stop codon at site 348 and 
            N-terminal 6x his tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6354)
  AUTHORS   Mehl R, van Zee K, Kean K
  TITLE     
  JOURNAL   2020
REFERENCE   2  (bases 1 to 6354)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "2020"
COMMENT     Sequence Label: pBad-HPII TAG348
FEATURES             Location/Qualifiers
     source          1..6354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        28..312
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     235..254
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             358..375
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             379..411
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(415..423,424..438)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(2782..2799)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      2952..3038
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3130..3157
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3176..3267
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3268..3336,3337..4128)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3486..3505)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4299..4887
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4788..4807
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5041..5058
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5073..5213
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5139..5158)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5299..5321
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(join(5477..6354,1))
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
ORIGIN
        1 tacttttcat actcccgcca ttcagagaag aaaccaattg tccatattgc atcagacatt
       61 gccgtcactg cgtcttttac tggctcttct cgctaaccaa accggtaacc ccgcttatta
      121 aaagcattct gtaacaaagc gggaccaaag ccatgacaaa aacgcgtaac aaaagtgtct
      181 ataatcacgg cagaaaagtc cacattgatt atttgcacgg cgtcacactt tgctatgcca
      241 tagcattttt atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta
      301 ctgtttctcc atacccgttt tttgggctaa caggaggaat taaccatggg gggttctcat
      361 catcatcatc atcatggtat ggctagcatg actggtggac agcaaatggg tcgggatctg
      421 tacgacgatg acgataagga tcgatgggga tccgagctcg agtcgcaaca taacgaaaag
      481 aacccacatc agcaccagtc accactacac gattccagcg aagcgaaacc ggggatggac
      541 tcactggcac ctgaggacgg ctctcatcgt ccagcggctg aaccaacacc gccaggtgca
      601 caacctaccg ccccagggag cctgaaagcc cctgatacgc gtaacgaaaa acttaattct
      661 ctggaagacg tacgcaaagg cagtgaaaat tatgcgctga ccactaatca gggcgtgcgc
      721 atcgccgacg atcaaaactc actgcgtgcc ggtagccgtg gtccaacgct gctggaagat
      781 tttattctgc gcgagaaaat cacccacttt gaccatgagc gcattccgga acgtattgtt
      841 catgcacgcg gatcagccgc tcacggttat ttccagccat ataaaagctt aagcgatatt
      901 accaaagcgg atttcctctc agatccgaac aaaatcaccc cagtatttgt acgtttctct
      961 accgttcagg gtggtgctgg ctctgctgat accgtgcgtg atatccgtgg ctttgccacc
     1021 aagttctata ccgaagaggg tatttttgac ctcgttggca ataacacgcc aatcttcttt
     1081 atccaggatg cgcataaatt ccccgatttt gttcatgcgg taaaaccaga accgcactgg
     1141 gcaattccac aagggcaaag tgcccacgat actttctggg attatgtttc tctgcaacct
     1201 gaaactctgc acaacgtgat gtgggcgatg tcggatcgcg gcatcccccg cagttaccgc
     1261 accatggaag gcttcggtat tcacaccttc cgcctgatta atgccgaagg gaaggcaacg
     1321 tttgtacgtt tccactggaa accactggca ggtaaagcct cactcgtttg ggatgaagca
     1381 caaaaactca ccggacgtga cccggacttc caccgccgcg agttgtggga agccattgaa
     1441 gcaggcgatt ttccggaata cgaactgggc ttccagttga ttcctgaaga agatgaattc
     1501 tagttcgact tcgatcttct cgatccaacc aaacttatcc cggaagaact ggtgcccgtt
     1561 cagcgtgtcg gcaaaatggt gctcaatcgc aacccggata acttctttgc tgaaaacgaa
     1621 caggcggctt tccatcctgg gcatatcgtg ccgggactgg acttcaccaa cgatccgctg
     1681 ttgcagggac gtttgttctc ctataccgat acacaaatca gtcgtcttgg tgggccgaat
     1741 ttccatgaga ttccgattaa ccgtccgacc tgcccttacc ataatttcca gcgtgacggc
     1801 atgcatcgca tggggatcga cactaacccg gcgaattacg aaccgaactc gattaacgat
     1861 aactggccgc gcgaaacacc gccggggccg aaacgcggcg gttttgaatc ataccaggag
     1921 cgcgtggaag gcaataaagt tcgcgagcgc agcccatcgt ttggcgaata ttattcccat
     1981 ccgcgtctgt tctggctaag tcagacgcca tttgagcagc gccatattgt cgatggtttc
     2041 agttttgagt taagcaaagt cgttcgtccg tatattcgtg agcgcgttgt tgaccagctg
     2101 gcgcatattg atctcactct ggcccaggcg gtggcgaaaa atctcggtat cgaactgact
     2161 gacgaccagc tgaatatcac cccacctccg gacgtcaacg gtctgaaaaa ggatccatcc
     2221 ttaagtttgt acgccattcc tgacggtgat gtgaaaggtc gcgtggtagc gattttactt
     2281 aatgatgaag tgagatcggc agaccttctg gccattctca aggcgctgaa ggccaaaggc
     2341 gttcatgcca aactgctcta ctcccgaatg ggtgaagtga ctgcggatga cggtacggtg
     2401 ttgcctatag ccgctacctt tgccggtgca ccttcgctga cggtcgatgc ggtcattgtc
     2461 ccttgcggca atatcgcgga tatcgctgac aacggcgatg ccaactacta cctgatggaa
     2521 gcctacaaac accttaaacc gattgcgctg gcgggtgacg cgcgcaagtt taaagcaaca
     2581 atcaagatcg ctgaccaggg tgaagaaggg attgtggaag ctgacagcgc tgacggtagt
     2641 tttatggatg aactgctaac gctgatggca gcacaccgcg tgtggtcacg cattcctaag
     2701 attgacaaaa ttcctgcctg atggggtacc atatgggaat tcgaagcttg gctgttttgg
     2761 cggatgagag aagattttca gcctgataca gattaaatca gaacgcagaa gcggtctgat
     2821 aaaacagaat ttgcctggcg gcagtagcgc ggtggtccca cctgacccca tgccgaactc
     2881 agaagtgaaa cgccgtagcg ccgatggtag tgtggggtct ccccatgcga gagtagggaa
     2941 ctgccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     3001 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
     3061 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccaggcatc
     3121 aaattaagca gaaggccatc ctgacggatg gcctttttgc gtttctacaa actcttttgt
     3181 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     3241 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     3301 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     3361 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     3421 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     3481 gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc
     3541 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     3601 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     3661 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     3721 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     3781 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     3841 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     3901 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     3961 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     4021 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     4081 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     4141 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     4201 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     4261 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     4321 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     4381 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     4441 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     4501 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     4561 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     4621 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     4681 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     4741 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     4801 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     4861 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     4921 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     4981 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     5041 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     5101 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca
     5161 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac
     5221 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     5281 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     5341 aacgcgcgag gcagcagatc aattcgcgcg cgaaggcgaa gcggcatgca taatgtgcct
     5401 gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg
     5461 tctgattcgt taccaattat gacaacttga cggctacatc attcactttt tcttcacaac
     5521 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc gagaaataga
     5581 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
     5641 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta
     5701 actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc
     5761 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
     5821 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
     5881 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
     5941 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
     6001 accccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
     6061 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
     6121 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
     6181 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
     6241 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
     6301 gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gcca
//
