LOCUS       sequence-205538-        9525 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9525)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9525)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-321
FEATURES             Location/Qualifiers
     source          1..9525
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     932..951
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(971..1048)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1254..1273
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1357..1376
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1357..1375
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1376..1400
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1454..1471
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5019..5108
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5121..5210
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5816..5834)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5830..5877
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5914..6369
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6001..6020)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6211..6232
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6461..7276)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6461..7276)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7184..7203
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7268..7287
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7398..7986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7887..7906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8140..8157
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8172..8311
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8237..8256)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8397..8419
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8413..8604)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9413..9525,1..970))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ccagtgacac gggcaacagc tgattgccct tcaccgcctg gccctgagag agttgcagca
       61 agcggtccac gctggtttgc cccagcaggc gaaaatcctg tttgatggtg gttaacggcg
      121 ggatataaca tgagctgtcc tcggtatcgt cgtatcccac taccgagata tccgcaccaa
      181 cgcgcagccc ggactcggta atggcgcgca ttgcgcccag cgccatctga tcgttggcaa
      241 ccagcatcgc agtgggaacg atgccctcat tcagcatttg catggtttgt tgaaaaccgg
      301 acatggcact ccagtcgcct tcccgttccg ctatcggctg aatttgattg cgagtgagat
      361 atttatgcca gccagccaga cgcagacgcg ccgagacaga acttaatggg cccgctaaca
      421 gcgcgatttg ctggtgaccc aatgcgacca gatgctccac gcccagtcgc gtaccgtcct
      481 catgggagaa aataatactg ttgatgggtg tctggtcaga gacatcaaga aataacgccg
      541 gaacattagt gcaggcagct tccacagcaa tggcatcctg gtcatccagc ggatagttaa
      601 tgatcagccc actgacgcgt tgcgcgagaa gattgtgcac cgccgcttta caggcttcga
      661 cgccgcttcg ttctaccatc gacaccacca cgctggcacc cagttgatcg gcgcgagatt
      721 taatcgccgc gacaatttgc gacggcgcgt gcagggccag actggaggtg gcaacgccaa
      781 tcagcaacga ctgtttgccc gccagttgtt gtgccacgcg gttgggaatg taattcagct
      841 ccgccatcgc cgcttccact ttttcccgcg ttttcgcaga aacgtggctg gcctggttca
      901 ccacgcggga aacggtctga taagagacac cggcatactc tgcgacatcg tataacgtta
      961 ctggtttcac attcaccacc ctgaattgac tctcttccgg gcgctatcat gccataccgc
     1021 gaaaggtttt gcgccattcg atggtgtccg ggatctcgac gctctccctt atgcgactcc
     1081 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     1141 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     1201 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     1261 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     1321 ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact ataggggaat
     1381 tgtgagcgga taacaattcc cctctagaaa taattttgcc ttaactttaa gaaggagata
     1441 taccatgggc agccaccacc accaccacca cagcggaatg cagatctttg ttaagactct
     1501 caccggaaag acaatcaccc tagaggtgga aagctccgac accatcgaca acgttaaggc
     1561 caagatccag gataaggagg gcattcctcc ggatcagcag aggcttattt tcgccggcaa
     1621 gcagctagag gatggccgta cgttggctga ttacaatatc cagaaggaat ccaccctcca
     1681 cttggtcctc aggctccgtg gtggttaagc ttgcaattaa ctttaagaag gagatataca
     1741 atgcttcaca agcgagctag tgaagctaac gataagaacg ataataccat catcggttcc
     1801 gatttagcat catctaagaa acgccgtatc gatttcactg aatcttcgtc tgataagtca
     1861 tcttcgattt tagcttctgg tagtagcagg ggttttcacg gcgatagcgt cgttcagcaa
     1921 atcgacatgg cttttggtaa ttcgaaccgt caggagattg atgaagatct gcacagtcgc
     1981 cagctcgccg tctatggtcg tgagactatg aggcgtctct ttgcttccaa tgttctcatc
     2041 tcggggatgc acggtcttgg tgccgagatt gccaagaatc ttatacttgc tggtgtgaag
     2101 tctgtgacct tgcatgatga aagagttgta gagctatggg acttatcaag caactttgtt
     2161 ttctcggagg atgatgttgg caagaataga gcagatgctt ctgttcagaa gttacaggat
     2221 cttaacaatg ctgtggttgt ctctagcttg accaaaagct taaacaaaga ggatctttct
     2281 ggcttccagg ttgttgtatt ctctgacata agcatggaaa gagcaatcga atttgatgac
     2341 tattgtcaca gccatcagcc tcctatcgct tttgtcaagg ctgatgtcag gggtcttttc
     2401 ggctctgtct tttgtgattt tgggcctgaa tttgcggttc ttgatgttga tggagaggaa
     2461 ccccatacag gcatcattgc gtctatctct aatgagaacc aggcatttat ttcctgtgtc
     2521 gacgatgaga ggcttgagtt tgaagatggt gacctagttg ttttctcaga agttgagggt
     2581 atgacagaat tgaatgatgg gaaaccaagg aagataaaaa gtacacggcc atattcattc
     2641 acccttgatg aggacactac taactatgga acatatgtga agggtggtat tgtcactcaa
     2701 gtgaaacagc caaagttact gaatttcaag ccattgaggg aagcgcttaa ggatccaggg
     2761 gattttctat ttagtgattt ctctaagttt gatcggcctc cactccttca cttagcattc
     2821 caggcacttg atcattttaa agctgaagct ggcagatttc ctgttgctgg ctcagaagag
     2881 gacgctcaga agcttatatc gattgccaca gccatcaata ccgggcaggg tgatttaaag
     2941 gtggagaatg ttgatcagaa gcttctaaga cacttttcct ttggagccaa ggctgtcctg
     3001 aatcctatgg ctgcaatgtt tggcggtatt gttggacagg aggttgttaa agcttgctct
     3061 ggaaaatttc atcctctctt ccagtttttc tactttgatt cagtggagtc actcccctct
     3121 gaacctgtgg actcgagtga ctttgcacca agaaacagcc ggtatgatgc ccaaatatct
     3181 gtatttgggg ccaagttcca gaagaaactt gaagatgcta aagttttcac agtagggtct
     3241 ggtgctcttg gctgtgagtt cttgaaaaat ttggctctga tgggggtttc ttgtggaagc
     3301 caagggaagc tgacagtgac agatgatgat atcattgaga agagtaacct cagtcgtcaa
     3361 tttctgttcc gtgactggaa cattggacag gctaaatcca cagttgctgc ttcggctgct
     3421 gctgttataa atcctcgctt caacattgag gccctgcaaa accgtgttgg cgcggagact
     3481 gagaatgtat ttgacgatgc cttctgggag aacttaactg ttgtcgtcaa tgcactggat
     3541 aatgttaatg cgaggctcta tgttgattcg aggtgcttgt atttccagaa gcctctcctt
     3601 gagtctggaa ctcttggtac aaagtgcaac acacagtcgg ttatcccaca tctaactgaa
     3661 aattacggtg cctcaaggga cccaccagag aaacaggccc ccatgtgtac agtgcattcc
     3721 ttcccgcata acattgatca ctgtttaact tgggctcgct ctgagtttga gggtctgctg
     3781 gagaaaactc ccgctgaagt gaatgcatat ctttctagcc cagttgagta cactaactca
     3841 atgatgagtg ctggtgatgc tcaggcgagg gacacattgg agaggattgt tgagtgcctt
     3901 gaaaaggaga agtgtgaaac cttccaagac tgcttaacct gggcacgact gaggtttgag
     3961 gattactttg tgaaccgtgt caagcaattg atatacacat ttcctgaaga tgctgcgact
     4021 agtaccggag ctccattctg gtctgctcca aaaagattcc ctcgtccact ccagtactcg
     4081 tcgtccgacc caagtctcct taactttatc acagcgactg ctattttacg agcagagaca
     4141 tttgggatcc cgatacctga gtggactaag aacccaaagg aagcagcaga agctgttgac
     4201 agagtgatag tcccagactt tgagccaagg caagatgcaa agattgtgac tgatgagaaa
     4261 gctaccactt taaccactgc ttcggtggac gacgctgcag tcatcgacga tctcattgct
     4321 aagatcgacc agtgtaggca taacttgtcg ccagacttca ggatgaaacc tattcagttc
     4381 gaaaaggatg atgatacaaa ctatcacatg gatgtgatag caggactagc caacatgagg
     4441 gcgagaaatt acagcatccc tgaagtggac aagctgaaag caaagttcat agcagggaga
     4501 atcatcccag ccattgcgac ttcaacagcc atggctactg ggctggtctg cctcgagctg
     4561 tacaaggtcc ttgatggagg acataaagtg gaagcttaca gaaacacatt tgccaacctg
     4621 gcacttccac tattctccat ggctgaacca cttccaccaa aggtggtgaa gcaccgtgac
     4681 atggcttgga cagtttggga cagatgggtt ctcaagggaa acccaactct tcgtgaggtg
     4741 ttgcagtggc tggaggacaa agggcttagc gcatacagca tctcttgtgg aagctgtcta
     4801 ctgttcaaca gtatgttcac aaggcacaag gagaggatgg acaagaaggt tgtggatctc
     4861 gctcgggatg ttgcgaaagt cgagttaccg ccttatcgca accatcttga tgtagtggtg
     4921 gcttgtgagg atgaagatga caatgacgtc gatattcctc tcgtctctat ctacttcagg
     4981 tgagcttact attaacttta agaaggagat ataccatgta cccatacgat gttcctgact
     5041 atgcgggcta tccctatgac gtcccggact atgcaggatt gtatccatat gacgttccag
     5101 attacgccac tagagctgct tacccatacg atgttcctga ctatgcgggc tatccctatg
     5161 acgtcccgga ctatgcagga ttgtatccat atgacgttcc agattacgca atggcgtcga
     5221 aaagaattaa caaagagctt agggacttgc aaagagatcc tcctgtgtcc tgcagtgcag
     5281 gtcctacggg agatgatatg ttccaatggc aagcaactat catgggtcca gcagatagcc
     5341 cgtttgccgg aggtgtgttt cttgttacca ttcacttccc accagattac cctttcaagc
     5401 caccaaaggt tgccttccgg accaaggttt atcacccaaa catcaacagt aatggaagta
     5461 tctgtcttga cattctgaaa gagcagtgga gccctgcact taccgtatcc aaggttcttc
     5521 tgtcgatatg ctcattgctg acggatccaa accctgacga tcctttggtt cctgaaatag
     5581 cgcacatcta caagacagac cgagtcaagt acgagtccac agctcaatcc tggactcaga
     5641 agtatgcaat gggatgagct tttacgcttt taagccagcc ccgacacccg ccaacacccg
     5701 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     5761 ggatgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
     5821 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
     5881 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     5941 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     6001 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     6061 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     6121 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     6181 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     6241 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     6301 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     6361 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6421 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca
     6481 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     6541 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     6601 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     6661 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     6721 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     6781 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agtcgaaata
     6841 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     6901 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     6961 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     7021 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     7081 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     7141 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     7201 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc
     7261 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     7321 ttcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     7381 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     7441 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     7501 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     7561 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     7621 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     7681 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     7741 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     7801 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     7861 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     7921 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     7981 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     8041 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     8101 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     8161 gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
     8221 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
     8281 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
     8341 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgactccgg
     8401 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta
     8461 aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag
     8521 ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag
     8581 ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg
     8641 ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca
     8701 tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca
     8761 gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca
     8821 gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt
     8881 ccgcgtttcc agactttacg aaacacggaa acccaagacc attcatgttg ttgctcaggt
     8941 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg
     9001 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat
     9061 gcgcacccgt ggggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt
     9121 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga
     9181 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc
     9241 tgccggcacc tgtcctacga gttgcatgat aaagaacaca gtcataagtg cggcgacgat
     9301 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg
     9361 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc
     9421 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     9481 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttca
//
