LOCUS       sequence-205539-        9543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9543)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-322
FEATURES             Location/Qualifiers
     source          1..9543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     956..975
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(995..1072)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1278..1297
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1381..1400
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1381..1399
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1400..1424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1478..1495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5043..5132
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5145..5234
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5858..5876)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5872..5919
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5956..6411
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6043..6062)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6253..6274
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6503..7318)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6503..7318)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7226..7245
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7310..7329
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7440..8028
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7929..7948
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8182..8199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8214..8353
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8279..8298)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8439..8461
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8455..8646)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9455..9543,1..994))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gcgccagggt ggtttttctt ttcaccagtg acacgggcaa cagctgattg cccttcaccg
       61 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
      121 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcctcggta tcgtcgtatc
      181 ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc
      241 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca
      301 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg
      361 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga
      421 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct
      481 ccacgcccag tcgcgtaccg tcctcatggg agaaaataat actgttgatg ggtgtctggt
      541 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat
      601 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt
      661 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg
      721 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg
      781 ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca
      841 cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg
      901 cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat
      961 actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt
     1021 ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct
     1081 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     1141 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
     1201 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     1261 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     1321 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
     1381 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
     1441 tgccttaact ttaagaagga gatataccat gggcagccac caccaccacc accacagcgg
     1501 aatgcagatc tttgttaaga ctctcaccgg aaagacaatc accctagagg tggaaagctc
     1561 cgacaccatc gacaacgtta aggccaagat ccaggataag gagggcattc ctccggatca
     1621 gcagaggctt attttcgccg gcaagcagct agaggatggc cgtacgttgg ctgattacaa
     1681 tatccagaag gaatccaccc tccacttggt cctcaggctc cgtggtggtt aagcttgcaa
     1741 ttaactttaa gaaggagata tacaatgctt cacaagcgag ctagtgaagc taacgataag
     1801 aacgataata ccatcatcgg ttccgattta gcatcatcta agaaacgccg tatcgatttc
     1861 actgaatctt cgtctgataa gtcatcttcg attttagctt ctggtagtag caggggtttt
     1921 cacggcgata gcgtcgttca gcaaatcgac atggcttttg gtaattcgaa ccgtcaggag
     1981 attgatgaag atctgcacag tcgccagctc gccgtctatg gtcgtgagac tatgaggcgt
     2041 ctctttgctt ccaatgttct catctcgggg atgcacggtc ttggtgccga gattgccaag
     2101 aatcttatac ttgctggtgt gaagtctgtg accttgcatg atgaaagagt tgtagagcta
     2161 tgggacttat caagcaactt tgttttctcg gaggatgatg ttggcaagaa tagagcagat
     2221 gcttctgttc agaagttaca ggatcttaac aatgctgtgg ttgtctctag cttgaccaaa
     2281 agcttaaaca aagaggatct ttctggcttc caggttgttg tattctctga cataagcatg
     2341 gaaagagcaa tcgaatttga tgactattgt cacagccatc agcctcctat cgcttttgtc
     2401 aaggctgatg tcaggggtct tttcggctct gtcttttgtg attttgggcc tgaatttgcg
     2461 gttcttgatg ttgatggaga ggaaccccat acaggcatca ttgcgtctat ctctaatgag
     2521 aaccaggcat ttatttcctg tgtcgacgat gagaggcttg agtttgaaga tggtgaccta
     2581 gttgttttct cagaagttga gggtatgaca gaattgaatg atgggaaacc aaggaagata
     2641 aaaagtacac ggccatattc attcaccctt gatgaggaca ctactaacta tggaacatat
     2701 gtgaagggtg gtattgtcac tcaagtgaaa cagccaaagt tactgaattt caagccattg
     2761 agggaagcgc ttaaggatcc aggggatttt ctatttagtg atttctctaa gtttgatcgg
     2821 cctccactcc ttcacttagc attccaggca cttgatcatt ttaaagctga agctggcaga
     2881 tttcctgttg ctggctcaga agaggacgct cagaagctta tatcgattgc cacagccatc
     2941 aataccgggc agggtgattt aaaggtggag aatgttgatc agaagcttct aagacacttt
     3001 tcctttggag ccaaggctgt cctgaatcct atggctgcaa tgtttggcgg tattgttgga
     3061 caggaggttg ttaaagcttg ctctggaaaa tttcatcctc tcttccagtt tttctacttt
     3121 gattcagtgg agtcactccc ctctgaacct gtggactcga gtgactttgc accaagaaac
     3181 agccggtatg atgcccaaat atctgtattt ggggccaagt tccagaagaa acttgaagat
     3241 gctaaagttt tcacagtagg gtctggtgct cttggctgtg agttcttgaa aaatttggct
     3301 ctgatggggg tttcttgtgg aagccaaggg aagctgacag tgacagatga tgatatcatt
     3361 gagaagagta acctcagtcg tcaatttctg ttccgtgact ggaacattgg acaggctaaa
     3421 tccacagttg ctgcttcggc tgctgctgtt ataaatcctc gcttcaacat tgaggccctg
     3481 caaaaccgtg ttggcgcgga gactgagaat gtatttgacg atgccttctg ggagaactta
     3541 actgttgtcg tcaatgcact ggataatgtt aatgcgaggc tctatgttga ttcgaggtgc
     3601 ttgtatttcc agaagcctct ccttgagtct ggaactcttg gtacaaagtg caacacacag
     3661 tcggttatcc cacatctaac tgaaaattac ggtgcctcaa gggacccacc agagaaacag
     3721 gcccccatgt gtacagtgca ttccttcccg cataacattg atcactgttt aacttgggct
     3781 cgctctgagt ttgagggtct gctggagaaa actcccgctg aagtgaatgc atatctttct
     3841 agcccagttg agtacactaa ctcaatgatg agtgctggtg atgctcaggc gagggacaca
     3901 ttggagagga ttgttgagtg ccttgaaaag gagaagtgtg aaaccttcca agactgctta
     3961 acctgggcac gactgaggtt tgaggattac tttgtgaacc gtgtcaagca attgatatac
     4021 acatttcctg aagatgctgc gactagtacc ggagctccat tctggtctgc tccaaaaaga
     4081 ttccctcgtc cactccagta ctcgtcgtcc gacccaagtc tccttaactt tatcacagcg
     4141 actgctattt tacgagcaga gacatttggg atcccgatac ctgagtggac taagaaccca
     4201 aaggaagcag cagaagctgt tgacagagtg atagtcccag actttgagcc aaggcaagat
     4261 gcaaagattg tgactgatga gaaagctacc actttaacca ctgcttcggt ggacgacgct
     4321 gcagtcatcg acgatctcat tgctaagatc gaccagtgta ggcataactt gtcgccagac
     4381 ttcaggatga aacctattca gttcgaaaag gatgatgata caaactatca catggatgtg
     4441 atagcaggac tagccaacat gagggcgaga aattacagca tccctgaagt ggacaagctg
     4501 aaagcaaagt tcatagcagg gagaatcatc ccagccattg cgacttcaac agccatggct
     4561 actgggctgg tctgcctcga gctgtacaag gtccttgatg gaggacataa agtggaagct
     4621 tacagaaaca catttgccaa cctggcactt ccactattct ccatggctga accacttcca
     4681 ccaaaggtgg tgaagcaccg tgacatggct tggacagttt gggacagatg ggttctcaag
     4741 ggaaacccaa ctcttcgtga ggtgttgcag tggctggagg acaaagggct tagcgcatac
     4801 agcatctctt gtggaagctg tctactgttc aacagtatgt tcacaaggca caaggagagg
     4861 atggacaaga aggttgtgga tctcgctcgg gatgttgcga aagtcgagtt accgccttat
     4921 cgcaaccatc ttgatgtagt ggtggcttgt gaggatgaag atgacaatga cgtcgatatt
     4981 cctctcgtct ctatctactt caggtgagct tactattaac tttaagaagg agatatacca
     5041 tgtacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag
     5101 gattgtatcc atatgacgtt ccagattacg ccactagagc tgcttaccca tacgatgttc
     5161 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggattgtat ccatatgacg
     5221 ttccagatta cgcaatgttt aagaaaatgg ataaaaaagc agcgcagaga attgcgatgg
     5281 aatacagagc tatgatctcg aaagaatctt tgttcagtat tggtcaaaac tcgaacaata
     5341 tatacgaatg gactgcagtg atccggggtc cagatggcac tccctatgaa ggtggcatgt
     5401 ttaatctcag tattaagttt cctacggatt atccttttaa accacccaag tttacgttta
     5461 aaactccgat ttaccatcca aatatcaatg atgaaggatc gatttgcatg aacattctta
     5521 aagacaaatg gactcctgct cttatggttg aaaaggtgct tctgtcaata cttttactat
     5581 tggaaaaacc aaacccagat gatcctcttg tacctgaaat tggacagctc ttcaagaata
     5641 acagattcca atttgaccag agagctcgag aattcactgc tcgacatgct aataattaag
     5701 cttttacgct tttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg
     5761 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgggatgaga tccggctgct
     5821 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
     5881 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
     5941 ggattggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     6001 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     6061 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     6121 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
     6181 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     6241 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
     6301 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     6361 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
     6421 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     6481 gtatccgctc atgaattaat tcttagaaaa actcatcgag catcaaatga aactgcaatt
     6541 tattcatatc aggattatca ataccatatt tttgaaaaag ccgtttctgt aatgaaggag
     6601 aaaactcacc gaggcagttc cataggatgg caagatcctg gtatcggtct gcgattccga
     6661 ctcgtccaac atcaatacaa cctattaatt tcccctcgtc aaaaataagg ttatcaagtg
     6721 agaaatcacc atgagtgacg actgaatccg gtgagaatgg caaaagttta tgcatttctt
     6781 tccagacttg ttcaacaggc cagccattac gctcgtcatc aaaatcactc gcatcaacca
     6841 aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa tacgcgatcg ctgttaaaag
     6901 gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa cactgccagc gcatcaacaa
     6961 tattttcacc tgaatcagga tattcttcta atacctggaa tgctgttttc ccggggatcg
     7021 cagtggtgag taaccatgca tcatcaggag tacggataaa atgcttgatg gtcggaagag
     7081 gcataaattc cgtcagccag tttagtctga ccatctcatc tgtaacatca ttggcaacgc
     7141 tacctttgcc atgtttcaga aacaactctg gcgcatcggg cttcccatac aatcgataga
     7201 ttgtcgcacc tgattgcccg acattatcgc gagcccattt atacccatat aaatcagcat
     7261 ccatgttgga atttaatcgc ggcctagagc aagacgtttc ccgttgaata tggctcataa
     7321 caccccttgt attactgttt atgtaagcag acagttttat tgttcatgac caaaatccct
     7381 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     7441 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     7501 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     7561 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
     7621 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     7681 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     7741 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     7801 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     7861 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     7921 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     7981 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     8041 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     8101 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     8161 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
     8221 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaatggtgc actctcagta
     8281 caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg
     8341 ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     8401 gctcccggca tccgcttaca gacaagctgt gaccgactcc gggagctgca tgtgtcagag
     8461 gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc
     8521 gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag
     8581 aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt
     8641 ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa
     8701 acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg
     8761 ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg
     8821 tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc
     8881 tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta
     8941 cgaaacacgg aaacccaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca
     9001 gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc
     9061 ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggggccgc
     9121 catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa
     9181 ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc
     9241 gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac
     9301 gagttgcatg ataaagaaca cagtcataag tgcggcgacg atagtcatgc cccgcgccca
     9361 ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc ccggtgccta
     9421 atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     9481 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     9541 tgg
//
