LOCUS       sequence-205541-       10008 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10008)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-323
FEATURES             Location/Qualifiers
     source          1..10008
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     956..975
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(995..1072)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1278..1297
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1381..1400
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1381..1399
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1400..1424
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1478..1495
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5043..5132
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5145..5234
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6323..6341)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6337..6384
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6421..6876
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6508..6527)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6718..6739
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6968..7783)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6968..7783)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7691..7710
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7775..7794
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7905..8493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8394..8413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8647..8664
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8679..8818
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8744..8763)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8904..8926
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8920..9111)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9920..10008,1..994))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gcgccagggt ggtttttctt ttcaccagtg acacgggcaa cagctgattg cccttcaccg
       61 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat
      121 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcctcggta tcgtcgtatc
      181 ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc
      241 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca
      301 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg
      361 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga
      421 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct
      481 ccacgcccag tcgcgtaccg tcctcatggg agaaaataat actgttgatg ggtgtctggt
      541 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat
      601 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt
      661 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg
      721 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg
      781 ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca
      841 cgcggttggg aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg
      901 cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat
      961 actctgcgac atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt
     1021 ccgggcgcta tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct
     1081 cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg
     1141 ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg
     1201 gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga
     1261 gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg
     1321 ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
     1381 taatacgact cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt
     1441 tgccttaact ttaagaagga gatataccat gggcagccac caccaccacc accacagcgg
     1501 aatgcagatc tttgttaaga ctctcaccgg aaagacaatc accctagagg tggaaagctc
     1561 cgacaccatc gacaacgtta aggccaagat ccaggataag gagggcattc ctccggatca
     1621 gcagaggctt attttcgccg gcaagcagct agaggatggc cgtacgttgg ctgattacaa
     1681 tatccagaag gaatccaccc tccacttggt cctcaggctc cgtggtggtt aagcttgcaa
     1741 ttaactttaa gaaggagata tacaatgctt cacaagcgag ctagtgaagc taacgataag
     1801 aacgataata ccatcatcgg ttccgattta gcatcatcta agaaacgccg tatcgatttc
     1861 actgaatctt cgtctgataa gtcatcttcg attttagctt ctggtagtag caggggtttt
     1921 cacggcgata gcgtcgttca gcaaatcgac atggcttttg gtaattcgaa ccgtcaggag
     1981 attgatgaag atctgcacag tcgccagctc gccgtctatg gtcgtgagac tatgaggcgt
     2041 ctctttgctt ccaatgttct catctcgggg atgcacggtc ttggtgccga gattgccaag
     2101 aatcttatac ttgctggtgt gaagtctgtg accttgcatg atgaaagagt tgtagagcta
     2161 tgggacttat caagcaactt tgttttctcg gaggatgatg ttggcaagaa tagagcagat
     2221 gcttctgttc agaagttaca ggatcttaac aatgctgtgg ttgtctctag cttgaccaaa
     2281 agcttaaaca aagaggatct ttctggcttc caggttgttg tattctctga cataagcatg
     2341 gaaagagcaa tcgaatttga tgactattgt cacagccatc agcctcctat cgcttttgtc
     2401 aaggctgatg tcaggggtct tttcggctct gtcttttgtg attttgggcc tgaatttgcg
     2461 gttcttgatg ttgatggaga ggaaccccat acaggcatca ttgcgtctat ctctaatgag
     2521 aaccaggcat ttatttcctg tgtcgacgat gagaggcttg agtttgaaga tggtgaccta
     2581 gttgttttct cagaagttga gggtatgaca gaattgaatg atgggaaacc aaggaagata
     2641 aaaagtacac ggccatattc attcaccctt gatgaggaca ctactaacta tggaacatat
     2701 gtgaagggtg gtattgtcac tcaagtgaaa cagccaaagt tactgaattt caagccattg
     2761 agggaagcgc ttaaggatcc aggggatttt ctatttagtg atttctctaa gtttgatcgg
     2821 cctccactcc ttcacttagc attccaggca cttgatcatt ttaaagctga agctggcaga
     2881 tttcctgttg ctggctcaga agaggacgct cagaagctta tatcgattgc cacagccatc
     2941 aataccgggc agggtgattt aaaggtggag aatgttgatc agaagcttct aagacacttt
     3001 tcctttggag ccaaggctgt cctgaatcct atggctgcaa tgtttggcgg tattgttgga
     3061 caggaggttg ttaaagcttg ctctggaaaa tttcatcctc tcttccagtt tttctacttt
     3121 gattcagtgg agtcactccc ctctgaacct gtggactcga gtgactttgc accaagaaac
     3181 agccggtatg atgcccaaat atctgtattt ggggccaagt tccagaagaa acttgaagat
     3241 gctaaagttt tcacagtagg gtctggtgct cttggctgtg agttcttgaa aaatttggct
     3301 ctgatggggg tttcttgtgg aagccaaggg aagctgacag tgacagatga tgatatcatt
     3361 gagaagagta acctcagtcg tcaatttctg ttccgtgact ggaacattgg acaggctaaa
     3421 tccacagttg ctgcttcggc tgctgctgtt ataaatcctc gcttcaacat tgaggccctg
     3481 caaaaccgtg ttggcgcgga gactgagaat gtatttgacg atgccttctg ggagaactta
     3541 actgttgtcg tcaatgcact ggataatgtt aatgcgaggc tctatgttga ttcgaggtgc
     3601 ttgtatttcc agaagcctct ccttgagtct ggaactcttg gtacaaagtg caacacacag
     3661 tcggttatcc cacatctaac tgaaaattac ggtgcctcaa gggacccacc agagaaacag
     3721 gcccccatgt gtacagtgca ttccttcccg cataacattg atcactgttt aacttgggct
     3781 cgctctgagt ttgagggtct gctggagaaa actcccgctg aagtgaatgc atatctttct
     3841 agcccagttg agtacactaa ctcaatgatg agtgctggtg atgctcaggc gagggacaca
     3901 ttggagagga ttgttgagtg ccttgaaaag gagaagtgtg aaaccttcca agactgctta
     3961 acctgggcac gactgaggtt tgaggattac tttgtgaacc gtgtcaagca attgatatac
     4021 acatttcctg aagatgctgc gactagtacc ggagctccat tctggtctgc tccaaaaaga
     4081 ttccctcgtc cactccagta ctcgtcgtcc gacccaagtc tccttaactt tatcacagcg
     4141 actgctattt tacgagcaga gacatttggg atcccgatac ctgagtggac taagaaccca
     4201 aaggaagcag cagaagctgt tgacagagtg atagtcccag actttgagcc aaggcaagat
     4261 gcaaagattg tgactgatga gaaagctacc actttaacca ctgcttcggt ggacgacgct
     4321 gcagtcatcg acgatctcat tgctaagatc gaccagtgta ggcataactt gtcgccagac
     4381 ttcaggatga aacctattca gttcgaaaag gatgatgata caaactatca catggatgtg
     4441 atagcaggac tagccaacat gagggcgaga aattacagca tccctgaagt ggacaagctg
     4501 aaagcaaagt tcatagcagg gagaatcatc ccagccattg cgacttcaac agccatggct
     4561 actgggctgg tctgcctcga gctgtacaag gtccttgatg gaggacataa agtggaagct
     4621 tacagaaaca catttgccaa cctggcactt ccactattct ccatggctga accacttcca
     4681 ccaaaggtgg tgaagcaccg tgacatggct tggacagttt gggacagatg ggttctcaag
     4741 ggaaacccaa ctcttcgtga ggtgttgcag tggctggagg acaaagggct tagcgcatac
     4801 agcatctctt gtggaagctg tctactgttc aacagtatgt tcacaaggca caaggagagg
     4861 atggacaaga aggttgtgga tctcgctcgg gatgttgcga aagtcgagtt accgccttat
     4921 cgcaaccatc ttgatgtagt ggtggcttgt gaggatgaag atgacaatga cgtcgatatt
     4981 cctctcgtct ctatctactt caggtgagct tactattaac tttaagaagg agatatacca
     5041 tgtacccata cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag
     5101 gattgtatcc atatgacgtt ccagattacg ccactagagc tgcttaccca tacgatgttc
     5161 ctgactatgc gggctatccc tatgacgtcc cggactatgc aggattgtat ccatatgacg
     5221 ttccagatta cgcaatggcg gatgagaggt ataatcggaa gaatccggcg gtgaagagga
     5281 ttttgcagga ggttaaggag atgcaagcta atccgtctga tgattttatg tctcttcctc
     5341 ttgaggagaa tatctttgag tggcaattcg ccatccgagg tcctggtgat actgaattcg
     5401 agggcgggat ttatcatggg agaattcagt tgcctgcaga ttatccattc aaacctcctt
     5461 cattcatgtt attgacccct aacgggcgct ttgaaactaa caccaagatt tgcttgagca
     5521 tttcaaacta ccaccctgag cattggcaac cttcatggag tgttcggact gctttggtgg
     5581 ctctcattgc atttatgccc acaagtccca atggagcact gggctcagta gattatccaa
     5641 aggatgagag acgtacacta gccattaaat cacgtgagac accacctaag tatggctctc
     5701 ctgaacggca aaaaattatt gatgagattc atcagtacat ccttagcaaa gcaaccgtgg
     5761 ttccaaaacc tcttcctctg gaatgtagcc aagcaccttc catcgtatca gaagctcact
     5821 cccaagttga gccacaggaa gcgataactg tagtagaaga gcggtccatc gctacaacag
     5881 acaccatagt tgatgatcaa atcatagaag agacagctga agcagtcaat acagcagcta
     5941 gtgtggttcc cgctgcagca cctttaccag cggttgaagt tgtggtcaaa gcttctgtaa
     6001 gtggtgagca aaggatggcc agaagagcgg ctcagaagcc agtcgatgac agactcttca
     6061 cgtgggcggc ggttgggctc acaatcgcta tcatggttct tcttctgaag aagttcataa
     6121 aatcaaatgg ttacagtact gggtttatgg atgatcagtc ttgagctttt acgcttttaa
     6181 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     6241 catccgctta cagacaagct gtgaccggga tgagatccgg ctgctaacaa agcccgaaag
     6301 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     6361 aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatt ggcgaatggg
     6421 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     6481 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6541 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6601 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6661 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6721 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6781 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6841 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
     6901 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgaa
     6961 ttaattctta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     7021 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     7081 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     7141 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     7201 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     7261 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     7321 gtgattgcgc ctgagcgagt cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     7381 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     7441 caggatattc ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc
     7501 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     7561 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     7621 tcagaaacaa ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt
     7681 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     7741 atcgcggcct agagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     7801 tgtttatgta agcagacagt tttattgttc atgaccaaaa tcccttaacg tgagttttcg
     7861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     7981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     8701 acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc tgctctgatg
     8761 ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc
     8821 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     8881 ttacagacaa gctgtgaccg actccgggag ctgcatgtgt cagaggtttt caccgtcatc
     8941 accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca
     9001 gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg
     9061 gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc
     9121 cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct
     9181 cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca
     9241 actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt
     9301 cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg
     9361 gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc
     9421 caagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt
     9481 tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg
     9541 ggtcctcaac gacaggagca cgatcatgcg cacccgtggg gccgccatgc cggcgataat
     9601 ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc
     9661 gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg
     9721 gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa
     9781 gaacacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac
     9841 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     9901 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     9961 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgg
//
