LOCUS       sequence-205542-        9776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression plasmid for codon optimized hSERT with internal
            MYC tag inserted in ECL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9776)
  AUTHORS   Young HJ, Chan M, Selvam B, Szymanski SK, Shukla D, Procko E
  TITLE     Deep Mutagenesis of a Transporter for Uptake of a Non-Native 
            Substrate Identifies Conformationally Dynamic Regions
  JOURNAL   bioRxiv 2021.04.19.440442
REFERENCE   2  (bases 1 to 9776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2021.04.19.440442"
COMMENT     Sequence Label: pCEP4-SERT-myc
FEATURES             Location/Qualifiers
     source          1..9776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(731..749)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        817..921
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(922..990,991..1782)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1140..1159)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1953..2541
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2442..2461
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2972..3117
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3160..4197
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(4195..4214)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4239..4287
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        4941..5320
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5321..5524
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5475..5495
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5521..5545
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      5573..5582
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6248..6277
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    7566..7700
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7590..7609)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7644..7663
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(7843..7865)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 cctgcccctc ccgctcctgc tcctgctcct gttccaccgt gggtcccttt gcagccaatg
       61 caacttggac gtttttgggg tctccggaca ccatctctat gtcttggccc tgatcctgag
      121 ccgcccgggg ctcctggtct tccgcctcct cgtcctcgtc ctcttccccg tcctcgtcca
      181 tggttatcac cccctcttct ttgaggtcca ctgccgccgg agccttctgg tccagatgtg
      241 tctcccttct ctcctaggcc atttccaggt cctgtacctg gcccctcgtc agacatgatt
      301 cacactaaaa gagatcaata gacatcttta ttagacgacg ctcagtgaat acagggagtg
      361 cagactcctg ccccctccaa cagccccccc accctcatcc ccttcatggt cgctgtcaga
      421 cagatccagg tctgaaaatt ccccatcctc cgaaccatcc tcgtcctcat caccaattac
      481 tcgcagcccg gaaaactccc gctgaacatc ctcaagattt gcgtcctgag cctcaagcca
      541 ggcctcaaat tcctcgtccc cctttttgct ggacggtagg gatggggatt ctcgggaccc
      601 ctcctcttcc tcttcaaggt caccagacag agatgctact ggggcaacgg aagaaaagct
      661 gggtgcggcc tgtgaggatc agcttatcga tgataagctg tcaaacatga gaattcttga
      721 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
      781 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      841 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      901 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      961 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     1021 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     1081 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     1141 ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata
     1201 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     1261 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     1321 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     1381 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     1441 gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact
     1501 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     1561 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     1621 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     1681 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1741 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1801 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1861 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1921 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1981 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     2041 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     2101 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     2161 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     2221 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     2281 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     2341 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     2401 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     2461 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     2521 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     2581 tgctggcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc
     2641 aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag
     2701 cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccccgag atgcgccgcg
     2761 tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat
     2821 tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga
     2881 ggtgccgccc tgcttcatcc ccgtggcccg ttgctcgcgt ttgctggcgg tgtccccgga
     2941 agaaatatat ttgcatgtct ttagttctat gatgacacaa accccgccca gcgtcttgtc
     3001 attggcgaat tcgaacacgc agatgcagtc ggggcggcgc ggtccgaggt ccacttcgca
     3061 tattaaggtg acgcgtgtgg cctcgaacac cgagcgaccc tgcagcgacc cgcttaacag
     3121 cgtcaacagc gtgccgcaga tcccgggggg caatgagata tgaaaaagcc tgaactcacc
     3181 gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag
     3241 ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc
     3301 ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt
     3361 gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg
     3421 acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa
     3481 ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt
     3541 agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg
     3601 cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac
     3661 gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac
     3721 tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac
     3781 aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac
     3841 gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc
     3901 tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc
     3961 cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct
     4021 tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca
     4081 caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat
     4141 agtggaaacc gacgccccag cactcgtccg gatcgggaga tgggggaggc taactgaaac
     4201 acggaaggag acaataccgg aaggaacccg cgctatgacg gcaataaaaa gacagaataa
     4261 aacgcacggg tgttgggtcg tttgttcata aacgcggggt tcggtcccag ggctggcact
     4321 ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct tccttttccc
     4381 caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag
     4441 gccctgccat agccactggc cccgtgggtt agggacgggg tcccccatgg ggaatggttt
     4501 atggttcgtg ggggttatta ttttgggcgt tgcgtggggt caggtccacg actggactga
     4561 gcagacagac ccatggtttt tggatggcct gggcatggac cgcatgtact ggcgcgacac
     4621 gaacaccggg cgtctgtggc tgccaaacac ccccgacccc caaaaaccac cgcgcggatt
     4681 tctggcgtgc caagctagtc gaccaattct catgtttgac agcttatcat cgcagatccg
     4741 ggcaacgttg ttgccattgc tgcaggcgca gaactggtag gtatggaaga tctatacatt
     4801 gaatcaatat tggcaattag ccatattagt cattggttat atagcataaa tcaatattgg
     4861 ctattggcca ttgcatacgt tgtatctata tcataatatg tacatttata ttggctcatg
     4921 tccaatatga ccgccatgtt gacattgatt attgactagt tattaatagt aatcaattac
     4981 ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg
     5041 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc
     5101 catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac
     5161 tgcccacttg gcagtacatc aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa
     5221 tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttacggg actttcctac
     5281 ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta
     5341 caccaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga
     5401 cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa
     5461 ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
     5521 agctcgttta gtgaaccgtc agatctctag aagctgggta ccagctgcta gcgccaccat
     5581 ggagaccacc cccctgaaca gtcaaaagca actctctgcc tgtgaggatg gagaggactg
     5641 tcaggagaat ggcgttcttc aaaaggttgt acccacccct ggagataagg tggaaagcgg
     5701 ccagatttcc aacggctaca gcgccgtgcc atcacctgga gccggggatg atacacggca
     5761 cagcattccc gcaaccacca cgacactggt tgcagagctt caccagggcg agagggagac
     5821 gtggggcaaa aaggtagatt ttctgctttc cgtgatcggt tacgcggtcg atctggggaa
     5881 cgtgtggagg ttcccttaca tttgttacca aaacggaggg ggtgccttct tgctgcccta
     5941 cacaattatg gcgatttttg ggggtatccc gctcttctac atggaactgg ccttgggtca
     6001 gtaccaccgg aacggctgca ttagcatttg gcgcaagata tgccccattt tcaagggaat
     6061 cggatacgca atctgtataa tcgcgttcta tattgcatca tattacaaca ccatcatggc
     6121 ctgggccctt tattacttga tcagcagttt caccgaccaa ttgccgtgga cgagctgtaa
     6181 gaactcatgg aataccggga actgtaccaa ctacttctct gaggacagct caggaggtag
     6241 cgggggtgaa cagaagctga ttagcgagga ggacttgggc agtccaggcg gaagcggcaa
     6301 catcacttgg acactccata gtacgagccc cgcagaggag ttttacacca ggcacgtact
     6361 gcagattcat aggagcaagg gtctgcaaga cctgggcggt attagctggc agctcgcatt
     6421 gtgcataatg ttgatcttta cggtgatcta tttctctatc tggaagggag taaagacatc
     6481 cggcaaggtg gtgtgggtga cggcgacctt cccctacatt atactcagcg tgttgttggt
     6541 gaggggggca accctgcctg gggcctggcg aggcgtgctg ttctatctca agccaaactg
     6601 gcagaagctt cttgaaaccg gagtgtggat cgacgcggcc gcgcaaatct tcttttccct
     6661 cggtcccggc ttcggggtcc ttctggcatt cgcaagctac aacaagttca acaataactg
     6721 ctaccaggac gccctcgtga caagtgtagt gaactgcatg acctctttcg tgagcgggtt
     6781 tgtgatcttc acagtactcg gatatatggc tgagatgagg aacgaagatg ttagtgaggt
     6841 tgccaaagac gctggcccga gcctgttgtt catcacatac gccgaggcta tcgcgaacat
     6901 gcccgcatct actttcttcg ccatcatctt tttcctgatg ctgataaccc ttgggctgga
     6961 tagcaccttc gccggactgg agggtgtcat cactgccgtt ctcgacgagt tcccccatgt
     7021 gtgggccaag cgacgggaaa ggttcgtgct ggccgtcgta atcacatgct tcttcggctc
     7081 actggtgacc cttacctttg gcggtgcgta cgtggtcaag ctgctcgaag agtacgccac
     7141 tggaccggcg gttctgaccg tggcattgat cgaggccgtg gccgtaagct ggttttacgg
     7201 catcacgcag ttctgtaggg acgtgaaaga gatgctgggc ttttctcccg gttggttttg
     7261 gaggatttgt tgggtagcaa ttagccccct gtttctcctt ttcattattt gctcttttct
     7321 gatgtctcca ccgcagctcc gactgtttca gtacaactac ccctactggt caattatcct
     7381 gggctattgt atcggcacct caagtttcat ctgcatcccc acatacatcg catacagact
     7441 gataattacg cccggtactt ttaaggagcg aatcattaaa agcataaccc ccgaaacccc
     7501 caccgagatc ccttgtggcg atataagact caatgcggtc taatagctcg aggccggcaa
     7561 ggccggatcc agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc
     7621 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
     7681 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
     7741 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
     7801 atgatccggc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     7861 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     7921 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg aggtcgactc tagaggatcg
     7981 atccccgccc cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca
     8041 gtgcatgtaa tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt
     8101 gacacggggg gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat
     8161 ggatgtgcac atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt
     8221 ggactgcaac acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc
     8281 tgggggacat gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat
     8341 cagaggggcc tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt
     8401 tataaggccc ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata
     8461 ctatccagac taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg
     8521 aattagggtt agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca
     8581 cggttttatt tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg
     8641 gcagtggctg aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc
     8701 attctccttc gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga
     8761 ggtgctcgaa aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca
     8821 gtggtggcat tgtgctatga caccaatata accctcacaa accccttggg caataaatac
     8881 tagtgtagga atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca
     8941 agccgtaaag actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc
     9001 tagtgcaata tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga
     9061 accatgttgt tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg
     9121 actccactgg ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc
     9181 cataaacaaa gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca
     9241 gcccccacac gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga
     9301 ttgtaacccc gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc
     9361 cggggaatac ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct
     9421 ggaggacaaa ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata
     9481 ttcacgaggt cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca
     9541 aatatctgga tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc
     9601 tatatctggg tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt
     9661 tatatctggg tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc
     9721 tatatctggg tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcct
//
