LOCUS       sequence-205544-        9792 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9792)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 9792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-325
FEATURES             Location/Qualifiers
     source          1..9792
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     72..91
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        72..90
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    91..115
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             169..186
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3734..3823
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3836..3925
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(4798..4816)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      4812..4859
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4896..5351
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4983..5002)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5193..5214
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5443..6258)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5443..6258)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6166..6185
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6250..6269
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6380..6968
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6869..6888
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7122..7139
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7154..7293
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7219..7238)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7379..7401
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7395..7586)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8395..9477)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9439..9458
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9478..9555)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9761..9780
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga
       61 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
      121 agaaataatt ttgccttaac tttaagaagg agatatacca tgggcagcca ccaccaccac
      181 caccacagcg gaatgcagat ctttgttaag actctcaccg gaaagacaat caccctagag
      241 gtggaaagct ccgacaccat cgacaacgtt aaggccaaga tccaggataa ggagggcatt
      301 cctccggatc agcagaggct tattttcgcc ggcaagcagc tagaggatgg ccgtacgttg
      361 gctgattaca atatccagaa ggaatccacc ctccacttgg tcctcaggct ccgtggtggt
      421 taagcttgca attaacttta agaaggagat atacaatgct tcacaagcga gctagtgaag
      481 ctaacgataa gaacgataat accatcatcg gttccgattt agcatcatct aagaaacgcc
      541 gtatcgattt cactgaatct tcgtctgata agtcatcttc gattttagct tctggtagta
      601 gcaggggttt tcacggcgat agcgtcgttc agcaaatcga catggctttt ggtaattcga
      661 accgtcagga gattgatgaa gatctgcaca gtcgccagct cgccgtctat ggtcgtgaga
      721 ctatgaggcg tctctttgct tccaatgttc tcatctcggg gatgcacggt cttggtgccg
      781 agattgccaa gaatcttata cttgctggtg tgaagtctgt gaccttgcat gatgaaagag
      841 ttgtagagct atgggactta tcaagcaact ttgttttctc ggaggatgat gttggcaaga
      901 atagagcaga tgcttctgtt cagaagttac aggatcttaa caatgctgtg gttgtctcta
      961 gcttgaccaa aagcttaaac aaagaggatc tttctggctt ccaggttgtt gtattctctg
     1021 acataagcat ggaaagagca atcgaatttg atgactattg tcacagccat cagcctccta
     1081 tcgcttttgt caaggctgat gtcaggggtc ttttcggctc tgtcttttgt gattttgggc
     1141 ctgaatttgc ggttcttgat gttgatggag aggaacccca tacaggcatc attgcgtcta
     1201 tctctaatga gaaccaggca tttatttcct gtgtcgacga tgagaggctt gagtttgaag
     1261 atggtgacct agttgttttc tcagaagttg agggtatgac agaattgaat gatgggaaac
     1321 caaggaagat aaaaagtaca cggccatatt cattcaccct tgatgaggac actactaact
     1381 atggaacata tgtgaagggt ggtattgtca ctcaagtgaa acagccaaag ttactgaatt
     1441 tcaagccatt gagggaagcg cttaaggatc caggggattt tctatttagt gatttctcta
     1501 agtttgatcg gcctccactc cttcacttag cattccaggc acttgatcat tttaaagctg
     1561 aagctggcag atttcctgtt gctggctcag aagaggacgc tcagaagctt atatcgattg
     1621 ccacagccat caataccggg cagggtgatt taaaggtgga gaatgttgat cagaagcttc
     1681 taagacactt ttcctttgga gccaaggctg tcctgaatcc tatggctgca atgtttggcg
     1741 gtattgttgg acaggaggtt gttaaagctt gctctggaaa atttcatcct ctcttccagt
     1801 ttttctactt tgattcagtg gagtcactcc cctctgaacc tgtggactcg agtgactttg
     1861 caccaagaaa cagccggtat gatgcccaaa tatctgtatt tggggccaag ttccagaaga
     1921 aacttgaaga tgctaaagtt ttcacagtag ggtctggtgc tcttggctgt gagttcttga
     1981 aaaatttggc tctgatgggg gtttcttgtg gaagccaagg gaagctgaca gtgacagatg
     2041 atgatatcat tgagaagagt aacctcagtc gtcaatttct gttccgtgac tggaacattg
     2101 gacaggctaa atccacagtt gctgcttcgg ctgctgctgt tataaatcct cgcttcaaca
     2161 ttgaggccct gcaaaaccgt gttggcgcgg agactgagaa tgtatttgac gatgccttct
     2221 gggagaactt aactgttgtc gtcaatgcac tggataatgt taatgcgagg ctctatgttg
     2281 attcgaggtg cttgtatttc cagaagcctc tccttgagtc tggaactctt ggtacaaagt
     2341 gcaacacaca gtcggttatc ccacatctaa ctgaaaatta cggtgcctca agggacccac
     2401 cagagaaaca ggcccccatg tgtacagtgc attccttccc gcataacatt gatcactgtt
     2461 taacttgggc tcgctctgag tttgagggtc tgctggagaa aactcccgct gaagtgaatg
     2521 catatctttc tagcccagtt gagtacacta actcaatgat gagtgctggt gatgctcagg
     2581 cgagggacac attggagagg attgttgagt gccttgaaaa ggagaagtgt gaaaccttcc
     2641 aagactgctt aacctgggca cgactgaggt ttgaggatta ctttgtgaac cgtgtcaagc
     2701 aattgatata cacatttcct gaagatgctg cgactagtac cggagctcca ttctggtctg
     2761 ctccaaaaag attccctcgt ccactccagt actcgtcgtc cgacccaagt ctccttaact
     2821 ttatcacagc gactgctatt ttacgagcag agacatttgg gatcccgata cctgagtgga
     2881 ctaagaaccc aaaggaagca gcagaagctg ttgacagagt gatagtccca gactttgagc
     2941 caaggcaaga tgcaaagatt gtgactgatg agaaagctac cactttaacc actgcttcgg
     3001 tggacgacgc tgcagtcatc gacgatctca ttgctaagat cgaccagtgt aggcataact
     3061 tgtcgccaga cttcaggatg aaacctattc agttcgaaaa ggatgatgat acaaactatc
     3121 acatggatgt gatagcagga ctagccaaca tgagggcgag aaattacagc atccctgaag
     3181 tggacaagct gaaagcaaag ttcatagcag ggagaatcat cccagccatt gcgacttcaa
     3241 cagccatggc tactgggctg gtctgcctcg agctgtacaa ggtccttgat ggaggacata
     3301 aagtggaagc ttacagaaac acatttgcca acctggcact tccactattc tccatggctg
     3361 aaccacttcc accaaaggtg gtgaagcacc gtgacatggc ttggacagtt tgggacagat
     3421 gggttctcaa gggaaaccca actcttcgtg aggtgttgca gtggctggag gacaaagggc
     3481 ttagcgcata cagcatctct tgtggaagct gtctactgtt caacagtatg ttcacaaggc
     3541 acaaggagag gatggacaag aaggttgtgg atctcgctcg ggatgttgcg aaagtcgagt
     3601 taccgcctta tcgcaaccat cttgatgtag tggtggcttg tgaggatgaa gatgacaatg
     3661 acgtcgatat tcctctcgtc tctatctact tcaggtgagc ttactattaa ctttaagaag
     3721 gagatatacc atgtacccat acgatgttcc tgactatgcg ggctatccct atgacgtccc
     3781 ggactatgca ggattgtatc catatgacgt tccagattac gccactagag ctgcttaccc
     3841 atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg caggattgta
     3901 tccatatgac gttccagatt acgcaatggc agaaaaggcc tgtataaaac gacttcaaaa
     3961 ggaatataga gcactttgca aggaaccagt ctcccatgtt gttgctcgtc cttccccaaa
     4021 tgacattctc gagtggcatt atgtgctgga aggcagtgag ggaacacctt ttgcaggtgg
     4081 attttactat ggaaagatca agtttcctcc cgagtatcct tacaagccac cagggattac
     4141 aatgactaca ccaaatggtc gattcatgac acagaagaaa atttgtttat ctatgagtga
     4201 ttttcatcca gaaagctgga atccgatgtg gtctgtatca agcatactta caggacttct
     4261 ctcatttatg atggatacca gtccgacaac tggaagtgtt aacactactg taattgagaa
     4321 acaacggttg gctaagtcat ctctcgcttt caattgtaaa accccagcat ttcgaaagct
     4381 atttccagag tatgtagaga agtacaacca gcagcaacta gctgagcaag ctaccacaca
     4441 actgacaaca ccagagtctc ctcaaaagag cgataccaaa gtcgagtcag agaaaaccat
     4501 cgatccaaca aagggagatt cagaaggtgg cttgaaggag aggaaaaaga acaataaaca
     4561 gggattgcca gcgtggataa tactgttgct agtgtcggtt ttcggtgtgg taatggcgtt
     4621 gcctctgctt caactgtgag cttttacgct tttaagccag ccccgacacc cgccaacacc
     4681 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     4741 cgggatgaga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
     4801 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
     4861 tgaaaggagg aactatatcc ggattggcga atgggacgcg ccctgtagcg gcgcattaag
     4921 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4981 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5041 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5101 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5161 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5221 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     5281 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5341 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     5401 ttctaaatac attcaaatat gtatccgctc atgaattaat tcttagaaaa actcatcgag
     5461 catcaaatga aactgcaatt tattcatatc aggattatca ataccatatt tttgaaaaag
     5521 ccgtttctgt aatgaaggag aaaactcacc gaggcagttc cataggatgg caagatcctg
     5581 gtatcggtct gcgattccga ctcgtccaac atcaatacaa cctattaatt tcccctcgtc
     5641 aaaaataagg ttatcaagtg agaaatcacc atgagtgacg actgaatccg gtgagaatgg
     5701 caaaagttta tgcatttctt tccagacttg ttcaacaggc cagccattac gctcgtcatc
     5761 aaaatcactc gcatcaacca aaccgttatt cattcgtgat tgcgcctgag cgagtcgaaa
     5821 tacgcgatcg ctgttaaaag gacaattaca aacaggaatc gaatgcaacc ggcgcaggaa
     5881 cactgccagc gcatcaacaa tattttcacc tgaatcagga tattcttcta atacctggaa
     5941 tgctgttttc ccggggatcg cagtggtgag taaccatgca tcatcaggag tacggataaa
     6001 atgcttgatg gtcggaagag gcataaattc cgtcagccag tttagtctga ccatctcatc
     6061 tgtaacatca ttggcaacgc tacctttgcc atgtttcaga aacaactctg gcgcatcggg
     6121 cttcccatac aatcgataga ttgtcgcacc tgattgcccg acattatcgc gagcccattt
     6181 atacccatat aaatcagcat ccatgttgga atttaatcgc ggcctagagc aagacgtttc
     6241 ccgttgaata tggctcataa caccccttgt attactgttt atgtaagcag acagttttat
     6301 tgttcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6361 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6421 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6481 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
     6541 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6601 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6661 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6721 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6781 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6841 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6901 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6961 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7021 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     7081 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     7141 aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     7201 gcaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact
     7261 ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac
     7321 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgactcc
     7381 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg
     7441 taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc
     7501 agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta
     7561 agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg
     7621 ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa
     7681 catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac
     7741 cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca
     7801 cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac
     7861 ttccgcgttt ccagacttta cgaaacacgg aaacccaaga ccattcatgt tgttgctcag
     7921 gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc
     7981 tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc
     8041 atgcgcaccc gtggggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg
     8101 gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc
     8161 gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc
     8221 gctgccggca cctgtcctac gagttgcatg ataaagaaca cagtcataag tgcggcgacg
     8281 atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc
     8341 ggtcgagatc ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg
     8401 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
     8461 gggagaggcg gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgacacggg
     8521 caacagctga ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct
     8581 ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga
     8641 gctgtcctcg gtatcgtcgt atcccactac cgagatatcc gcaccaacgc gcagcccgga
     8701 ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt
     8761 gggaacgatg ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca
     8821 gtcgccttcc cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc
     8881 agccagacgc agacgcgccg agacagaact taatgggccc gctaacagcg cgatttgctg
     8941 gtgacccaat gcgaccagat gctccacgcc cagtcgcgta ccgtcctcat gggagaaaat
     9001 aatactgttg atgggtgtct ggtcagagac atcaagaaat aacgccggaa cattagtgca
     9061 ggcagcttcc acagcaatgg catcctggtc atccagcgga tagttaatga tcagcccact
     9121 gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag gcttcgacgc cgcttcgttc
     9181 taccatcgac accaccacgc tggcacccag ttgatcggcg cgagatttaa tcgccgcgac
     9241 aatttgcgac ggcgcgtgca gggccagact ggaggtggca acgccaatca gcaacgactg
     9301 tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa ttcagctccg ccatcgccgc
     9361 ttccactttt tcccgcgttt tcgcagaaac gtggctggcc tggttcacca cgcgggaaac
     9421 ggtctgataa gagacaccgg catactctgc gacatcgtat aacgttactg gtttcacatt
     9481 caccaccctg aattgactct cttccgggcg ctatcatgcc ataccgcgaa aggttttgcg
     9541 ccattcgatg gtgtccggga tctcgacgct ctcccttatg cgactcctgc attaggaagc
     9601 agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg
     9661 agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag
     9721 cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg
     9781 cgccagcaac cg
//
