LOCUS       sequence-205546-       10049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-331
FEATURES             Location/Qualifiers
     source          1..10049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     951..970
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(990..1067)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1273..1292
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1376..1395
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1376..1394
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1395..1419
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1473..1490
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5038..5127
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5140..5229
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6359..6377)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6373..6420
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6457..6912
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6544..6563)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6754..6775
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7004..7819)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7004..7819)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7727..7746
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7811..7830
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7941..8529
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8430..8449
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8683..8700
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8715..8854
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8780..8799)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8940..8962
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8956..9147)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9956..10049,1..989))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agggtggttt ttcttttcac cagtgacacg ggcaacagct gattgccctt caccgcctgg
       61 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
      121 ttgatggtgg ttaacggcgg gatataacat gagctgtcct cggtatcgtc gtatcccact
      181 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
      241 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
      301 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
      361 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
      421 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
      481 cccagtcgcg taccgtcctc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
      541 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
      601 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
      661 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
      721 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
      781 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
      841 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
      901 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
      961 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
     1021 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
     1081 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
     1141 caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac
     1201 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg
     1261 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt
     1321 gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaattaata
     1381 cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgcct
     1441 taactttaag aaggagatat accatgggca gccaccacca ccaccaccac agcggaatgc
     1501 agatctttgt taagactctc accggaaaga caatcaccct agaggtggaa agctccgaca
     1561 ccatcgacaa cgttaaggcc aagatccagg ataaggaggg cattcctccg gatcagcaga
     1621 ggcttatttt cgccggcaag cagctagagg atggccgtac gttggctgat tacaatatcc
     1681 agaaggaatc caccctccac ttggtcctca ggctccgtgg tggttaagct tgcaattaac
     1741 tttaagaagg agatatacaa tgcttcacaa gcgagctagt gaagctaacg ataagaacga
     1801 taataccatc atcggttccg atttagcatc atctaagaaa cgccgtatcg atttcactga
     1861 atcttcgtct gataagtcat cttcgatttt agcttctggt agtagcaggg gttttcacgg
     1921 cgatagcgtc gttcagcaaa tcgacatggc ttttggtaat tcgaaccgtc aggagattga
     1981 tgaagatctg cacagtcgcc agctcgccgt ctatggtcgt gagactatga ggcgtctctt
     2041 tgcttccaat gttctcatct cggggatgca cggtcttggt gccgagattg ccaagaatct
     2101 tatacttgct ggtgtgaagt ctgtgacctt gcatgatgaa agagttgtag agctatggga
     2161 cttatcaagc aactttgttt tctcggagga tgatgttggc aagaatagag cagatgcttc
     2221 tgttcagaag ttacaggatc ttaacaatgc tgtggttgtc tctagcttga ccaaaagctt
     2281 aaacaaagag gatctttctg gcttccaggt tgttgtattc tctgacataa gcatggaaag
     2341 agcaatcgaa tttgatgact attgtcacag ccatcagcct cctatcgctt ttgtcaaggc
     2401 tgatgtcagg ggtcttttcg gctctgtctt ttgtgatttt gggcctgaat ttgcggttct
     2461 tgatgttgat ggagaggaac cccatacagg catcattgcg tctatctcta atgagaacca
     2521 ggcatttatt tcctgtgtcg acgatgagag gcttgagttt gaagatggtg acctagttgt
     2581 tttctcagaa gttgagggta tgacagaatt gaatgatggg aaaccaagga agataaaaag
     2641 tacacggcca tattcattca cccttgatga ggacactact aactatggaa catatgtgaa
     2701 gggtggtatt gtcactcaag tgaaacagcc aaagttactg aatttcaagc cattgaggga
     2761 agcgcttaag gatccagggg attttctatt tagtgatttc tctaagtttg atcggcctcc
     2821 actccttcac ttagcattcc aggcacttga tcattttaaa gctgaagctg gcagatttcc
     2881 tgttgctggc tcagaagagg acgctcagaa gcttatatcg attgccacag ccatcaatac
     2941 cgggcagggt gatttaaagg tggagaatgt tgatcagaag cttctaagac acttttcctt
     3001 tggagccaag gctgtcctga atcctatggc tgcaatgttt ggcggtattg ttggacagga
     3061 ggttgttaaa gcttgctctg gaaaatttca tcctctcttc cagtttttct actttgattc
     3121 agtggagtca ctcccctctg aacctgtgga ctcgagtgac tttgcaccaa gaaacagccg
     3181 gtatgatgcc caaatatctg tatttggggc caagttccag aagaaacttg aagatgctaa
     3241 agttttcaca gtagggtctg gtgctcttgg ctgtgagttc ttgaaaaatt tggctctgat
     3301 gggggtttct tgtggaagcc aagggaagct gacagtgaca gatgatgata tcattgagaa
     3361 gagtaacctc agtcgtcaat ttctgttccg tgactggaac attggacagg ctaaatccac
     3421 agttgctgct tcggctgctg ctgttataaa tcctcgcttc aacattgagg ccctgcaaaa
     3481 ccgtgttggc gcggagactg agaatgtatt tgacgatgcc ttctgggaga acttaactgt
     3541 tgtcgtcaat gcactggata atgttaatgc gaggctctat gttgattcga ggtgcttgta
     3601 tttccagaag cctctccttg agtctggaac tcttggtaca aagtgcaaca cacagtcggt
     3661 tatcccacat ctaactgaaa attacggtgc ctcaagggac ccaccagaga aacaggcccc
     3721 catgtgtaca gtgcattcct tcccgcataa cattgatcac tgtttaactt gggctcgctc
     3781 tgagtttgag ggtctgctgg agaaaactcc cgctgaagtg aatgcatatc tttctagccc
     3841 agttgagtac actaactcaa tgatgagtgc tggtgatgct caggcgaggg acacattgga
     3901 gaggattgtt gagtgccttg aaaaggagaa gtgtgaaacc ttccaagact gcttaacctg
     3961 ggcacgactg aggtttgagg attactttgt gaaccgtgtc aagcaattga tatacacatt
     4021 tcctgaagat gctgcgacta gtaccggagc tccattctgg tctgctccaa aaagattccc
     4081 tcgtccactc cagtactcgt cgtccgaccc aagtctcctt aactttatca cagcgactgc
     4141 tattttacga gcagagacat ttgggatccc gatacctgag tggactaaga acccaaagga
     4201 agcagcagaa gctgttgaca gagtgatagt cccagacttt gagccaaggc aagatgcaaa
     4261 gattgtgact gatgagaaag ctaccacttt aaccactgct tcggtggacg acgctgcagt
     4321 catcgacgat ctcattgcta agatcgacca gtgtaggcat aacttgtcgc cagacttcag
     4381 gatgaaacct attcagttcg aaaaggatga tgatacaaac tatcacatgg atgtgatagc
     4441 aggactagcc aacatgaggg cgagaaatta cagcatccct gaagtggaca agctgaaagc
     4501 aaagttcata gcagggagaa tcatcccagc cattgcgact tcaacagcca tggctactgg
     4561 gctggtctgc ctcgagctgt acaaggtcct tgatggagga cataaagtgg aagcttacag
     4621 aaacacattt gccaacctgg cacttccact attctccatg gctgaaccac ttccaccaaa
     4681 ggtggtgaag caccgtgaca tggcttggac agtttgggac agatgggttc tcaagggaaa
     4741 cccaactctt cgtgaggtgt tgcagtggct ggaggacaaa gggcttagcg catacagcat
     4801 ctcttgtgga agctgtctac tgttcaacag tatgttcaca aggcacaagg agaggatgga
     4861 caagaaggtt gtggatctcg ctcgggatgt tgcgaaagtc gagttaccgc cttatcgcaa
     4921 ccatcttgat gtagtggtgg cttgtgagga tgaagatgac aatgacgtcg atattcctct
     4981 cgtctctatc tacttcaggt gagcttacta ttaactttaa gaaggagata taccatgtac
     5041 ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggattg
     5101 tatccatatg acgttccaga ttacgccact agagctgctt acccatacga tgttcctgac
     5161 tatgcgggct atccctatga cgtcccggac tatgcaggat tgtatccata tgacgttcca
     5221 gattacgcaa tggccaacag taatttgccg cgaagaatca tcaaggaaac tcaacgtctg
     5281 ctcagtgaac cggctcccgg tataagtgcc tctccatctg aggataatat gcggtatttc
     5341 aacgttatga ttcttggtcc tacacaatca ccttatgaag gaggagtttt caagttggag
     5401 ctctttttgc ctgaagaata ccctatggca gctcccaagg ttaggtttct cacaaagata
     5461 taccatccta acattgacaa gcttggaaga atctgtcttg atattctcaa ggacaaatgg
     5521 agccctgcac tacaaatacg aacagtgctc ttaagtattc aagctcttct gagtgcacca
     5581 aacccagatg atccgctttc tgagaacatt gctaagcact ggaagagtaa tgaggcagaa
     5641 gctgtggata cagctaagga atggacccgt ctttatgcaa gcggcgcatg agcttttact
     5701 taactttaag aaggagatat acaatgagtt cggaggaagc caaggtcgtt gttccgagga
     5761 actttagatt gttggaagag cttgaaagag gtgagaaagg tattggagat ggtactgtta
     5821 gctatggaat ggatgatgct gatgatattt atatgcaatc ttggactggc accattcttg
     5881 gtcctcctaa tactgcatat gaagggaaaa tctttcagct gaagcttttc tgtggtaagg
     5941 agtaccctga aagtccaccg actgtgaggt tccagacccg gataaacatg gcttgtgtta
     6001 acccagaaac tggagtggtt gaaccgagtc tcttccctat gctcactaac tggcggcgag
     6061 aatatacaat ggaggacata ctggttaagc tgaaaaaaga aatgatgact tcgcataacc
     6121 ggaagttagc tcaacccccg gaaggtaacg aggaagctag ggcagatcca aaaggaccag
     6181 ctaaatgttg tgtgatgtga gcttcagagc ttttaagcca gccccgacac ccgccaacac
     6241 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     6301 ccgggatgag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc
     6361 gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg
     6421 ctgaaaggag gaactatatc cggattggcg aatgggacgc gccctgtagc ggcgcattaa
     6481 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6541 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6601 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6661 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6721 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6781 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6841 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6901 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6961 tttctaaata cattcaaata tgtatccgct catgaattaa ttcttagaaa aactcatcga
     7021 gcatcaaatg aaactgcaat ttattcatat caggattatc aataccatat ttttgaaaaa
     7081 gccgtttctg taatgaagga gaaaactcac cgaggcagtt ccataggatg gcaagatcct
     7141 ggtatcggtc tgcgattccg actcgtccaa catcaataca acctattaat ttcccctcgt
     7201 caaaaataag gttatcaagt gagaaatcac catgagtgac gactgaatcc ggtgagaatg
     7261 gcaaaagttt atgcatttct ttccagactt gttcaacagg ccagccatta cgctcgtcat
     7321 caaaatcact cgcatcaacc aaaccgttat tcattcgtga ttgcgcctga gcgagtcgaa
     7381 atacgcgatc gctgttaaaa ggacaattac aaacaggaat cgaatgcaac cggcgcagga
     7441 acactgccag cgcatcaaca atattttcac ctgaatcagg atattcttct aatacctgga
     7501 atgctgtttt cccggggatc gcagtggtga gtaaccatgc atcatcagga gtacggataa
     7561 aatgcttgat ggtcggaaga ggcataaatt ccgtcagcca gtttagtctg accatctcat
     7621 ctgtaacatc attggcaacg ctacctttgc catgtttcag aaacaactct ggcgcatcgg
     7681 gcttcccata caatcgatag attgtcgcac ctgattgccc gacattatcg cgagcccatt
     7741 tatacccata taaatcagca tccatgttgg aatttaatcg cggcctagag caagacgttt
     7801 cccgttgaat atggctcata acaccccttg tattactgtt tatgtaagca gacagtttta
     7861 ttgttcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7921 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7981 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8041 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     8101 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8161 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8221 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8281 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8341 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8401 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8461 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8521 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     8581 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8641 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8701 gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     8761 cgcaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac
     8821 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
     8881 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgactc
     8941 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg
     9001 gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc
     9061 cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt
     9121 aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat
     9181 gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga
     9241 acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga
     9301 ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc
     9361 acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga
     9421 cttccgcgtt tccagacttt acgaaacacg gaaacccaag accattcatg ttgttgctca
     9481 ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt
     9541 ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat
     9601 catgcgcacc cgtggggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt
     9661 ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag
     9721 cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag
     9781 cgctgccggc acctgtccta cgagttgcat gataaagaac acagtcataa gtgcggcgac
     9841 gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat
     9901 cggtcgagat cccggtgcct aatgagtgag ctaacttaca ttaattgcgt tgcgctcact
     9961 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
    10021 ggggagaggc ggtttgcgta ttgggcgcc
//
