LOCUS       sequence-205548-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-333
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     563..582
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(602..679)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     885..904
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     988..1007
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        988..1006
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1007..1031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1085..1102
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4650..4739
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4752..4841
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5935..5953)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5949..5996
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6033..6488
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6120..6139)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6330..6351
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6580..7395)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6580..7395)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7303..7322
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7387..7406
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7517..8105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8006..8025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8259..8276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8291..8430
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8356..8375)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8516..8538
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8532..8723)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9532..10013,1..601))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 agccagccag acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt
       61 gctggtgacc caatgcgacc agatgctcca cgcccagtcg cgtaccgtcc tcatgggaga
      121 aaataatact gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag
      181 tgcaggcagc ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc
      241 cactgacgcg ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc
      301 gttctaccat cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg
      361 cgacaatttg cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg
      421 actgtttgcc cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg
      481 ccgcttccac tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg
      541 aaacggtctg ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca
      601 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
      661 tgcgccattc gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg
      721 aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc
      781 aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa
      841 caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatcgaga tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg
     1021 ataacaattc ccctctagaa ataattttgc cttaacttta agaaggagat ataccatggg
     1081 cagccaccac caccaccacc acagcggaat gcagatcttt gttaagactc tcaccggaaa
     1141 gacaatcacc ctagaggtgg aaagctccga caccatcgac aacgttaagg ccaagatcca
     1201 ggataaggag ggcattcctc cggatcagca gaggcttatt ttcgccggca agcagctaga
     1261 ggatggccgt acgttggctg attacaatat ccagaaggaa tccaccctcc acttggtcct
     1321 caggctccgt ggtggttaag cttgcaatta actttaagaa ggagatatac aatgcttcac
     1381 aagcgagcta gtgaagctaa cgataagaac gataatacca tcatcggttc cgatttagca
     1441 tcatctaaga aacgccgtat cgatttcact gaatcttcgt ctgataagtc atcttcgatt
     1501 ttagcttctg gtagtagcag gggttttcac ggcgatagcg tcgttcagca aatcgacatg
     1561 gcttttggta attcgaaccg tcaggagatt gatgaagatc tgcacagtcg ccagctcgcc
     1621 gtctatggtc gtgagactat gaggcgtctc tttgcttcca atgttctcat ctcggggatg
     1681 cacggtcttg gtgccgagat tgccaagaat cttatacttg ctggtgtgaa gtctgtgacc
     1741 ttgcatgatg aaagagttgt agagctatgg gacttatcaa gcaactttgt tttctcggag
     1801 gatgatgttg gcaagaatag agcagatgct tctgttcaga agttacagga tcttaacaat
     1861 gctgtggttg tctctagctt gaccaaaagc ttaaacaaag aggatctttc tggcttccag
     1921 gttgttgtat tctctgacat aagcatggaa agagcaatcg aatttgatga ctattgtcac
     1981 agccatcagc ctcctatcgc ttttgtcaag gctgatgtca ggggtctttt cggctctgtc
     2041 ttttgtgatt ttgggcctga atttgcggtt cttgatgttg atggagagga accccataca
     2101 ggcatcattg cgtctatctc taatgagaac caggcattta tttcctgtgt cgacgatgag
     2161 aggcttgagt ttgaagatgg tgacctagtt gttttctcag aagttgaggg tatgacagaa
     2221 ttgaatgatg ggaaaccaag gaagataaaa agtacacggc catattcatt cacccttgat
     2281 gaggacacta ctaactatgg aacatatgtg aagggtggta ttgtcactca agtgaaacag
     2341 ccaaagttac tgaatttcaa gccattgagg gaagcgctta aggatccagg ggattttcta
     2401 tttagtgatt tctctaagtt tgatcggcct ccactccttc acttagcatt ccaggcactt
     2461 gatcatttta aagctgaagc tggcagattt cctgttgctg gctcagaaga ggacgctcag
     2521 aagcttatat cgattgccac agccatcaat accgggcagg gtgatttaaa ggtggagaat
     2581 gttgatcaga agcttctaag acacttttcc tttggagcca aggctgtcct gaatcctatg
     2641 gctgcaatgt ttggcggtat tgttggacag gaggttgtta aagcttgctc tggaaaattt
     2701 catcctctct tccagttttt ctactttgat tcagtggagt cactcccctc tgaacctgtg
     2761 gactcgagtg actttgcacc aagaaacagc cggtatgatg cccaaatatc tgtatttggg
     2821 gccaagttcc agaagaaact tgaagatgct aaagttttca cagtagggtc tggtgctctt
     2881 ggctgtgagt tcttgaaaaa tttggctctg atgggggttt cttgtggaag ccaagggaag
     2941 ctgacagtga cagatgatga tatcattgag aagagtaacc tcagtcgtca atttctgttc
     3001 cgtgactgga acattggaca ggctaaatcc acagttgctg cttcggctgc tgctgttata
     3061 aatcctcgct tcaacattga ggccctgcaa aaccgtgttg gcgcggagac tgagaatgta
     3121 tttgacgatg ccttctggga gaacttaact gttgtcgtca atgcactgga taatgttaat
     3181 gcgaggctct atgttgattc gaggtgcttg tatttccaga agcctctcct tgagtctgga
     3241 actcttggta caaagtgcaa cacacagtcg gttatcccac atctaactga aaattacggt
     3301 gcctcaaggg acccaccaga gaaacaggcc cccatgtgta cagtgcattc cttcccgcat
     3361 aacattgatc actgtttaac ttgggctcgc tctgagtttg agggtctgct ggagaaaact
     3421 cccgctgaag tgaatgcata tctttctagc ccagttgagt acactaactc aatgatgagt
     3481 gctggtgatg ctcaggcgag ggacacattg gagaggattg ttgagtgcct tgaaaaggag
     3541 aagtgtgaaa ccttccaaga ctgcttaacc tgggcacgac tgaggtttga ggattacttt
     3601 gtgaaccgtg tcaagcaatt gatatacaca tttcctgaag atgctgcgac tagtaccgga
     3661 gctccattct ggtctgctcc aaaaagattc cctcgtccac tccagtactc gtcgtccgac
     3721 ccaagtctcc ttaactttat cacagcgact gctattttac gagcagagac atttgggatc
     3781 ccgatacctg agtggactaa gaacccaaag gaagcagcag aagctgttga cagagtgata
     3841 gtcccagact ttgagccaag gcaagatgca aagattgtga ctgatgagaa agctaccact
     3901 ttaaccactg cttcggtgga cgacgctgca gtcatcgacg atctcattgc taagatcgac
     3961 cagtgtaggc ataacttgtc gccagacttc aggatgaaac ctattcagtt cgaaaaggat
     4021 gatgatacaa actatcacat ggatgtgata gcaggactag ccaacatgag ggcgagaaat
     4081 tacagcatcc ctgaagtgga caagctgaaa gcaaagttca tagcagggag aatcatccca
     4141 gccattgcga cttcaacagc catggctact gggctggtct gcctcgagct gtacaaggtc
     4201 cttgatggag gacataaagt ggaagcttac agaaacacat ttgccaacct ggcacttcca
     4261 ctattctcca tggctgaacc acttccacca aaggtggtga agcaccgtga catggcttgg
     4321 acagtttggg acagatgggt tctcaaggga aacccaactc ttcgtgaggt gttgcagtgg
     4381 ctggaggaca aagggcttag cgcatacagc atctcttgtg gaagctgtct actgttcaac
     4441 agtatgttca caaggcacaa ggagaggatg gacaagaagg ttgtggatct cgctcgggat
     4501 gttgcgaaag tcgagttacc gccttatcgc aaccatcttg atgtagtggt ggcttgtgag
     4561 gatgaagatg acaatgacgt cgatattcct ctcgtctcta tctacttcag gtgagcttac
     4621 tattaacttt aagaaggaga tataccatgt acccatacga tgttcctgac tatgcgggct
     4681 atccctatga cgtcccggac tatgcaggat tgtatccata tgacgttcca gattacgcca
     4741 ctagagctgc ttacccatac gatgttcctg actatgcggg ctatccctat gacgtcccgg
     4801 actatgcagg attgtatcca tatgacgttc cagattacgc aatggccaac agtaatttgc
     4861 cgcgaagaat catcaaggaa actcaacgtc tgctcagtga accggctccc ggtataagtg
     4921 cctctccatc tgaggataat atgcggtatt tcaacgttat gattcttggt cctacacaat
     4981 caccttatga aggaggagtt ttcaagttgg agctcttttt gcctgaagaa taccctatgg
     5041 cagctcccaa ggttaggttt ctcacaaaga tataccatcc taacattgac aagcttggaa
     5101 gaatctgtct tgatattctc aaggacaaat ggagccctgc actacaaata cgaacagtgc
     5161 tcttaagtat tcaagctctt ctgagtgcac caaacccaga tgatccgctt tctgagaaca
     5221 ttgctaagca ctggaagagt aatgaggcag aagctgtgga tacagctaag gaatggaccc
     5281 gtctttatgc aagcggcgca tgagctttta cttaacttta agaaggagat atacaatgac
     5341 tcttggctca ggatcgagtg ttgtcgttcc gaggaatttc cggttgctgg aggagcttga
     5401 acgtggagag aaaggtattg gagatggaac tgtgagctat ggaatggatg atggagatga
     5461 catttatatg cgctcttgga ctggcactat catcggtcct cacaacgtaa ctgtacatga
     5521 gggtcggatt tatcagttga agctcttctg tgacaaagat taccctgaga aacctccgac
     5581 tgttcggttc cattcgcgta tcaacatgac ttgtgtcaac catgataccg gcgtggtgga
     5641 ttcaaagaag tttggggttc ttgcaaactg gcaaagacag tacacaatgg aggacatact
     5701 gactcaattg aagaaggaga tggctgcatc gcataaccgg aagcttgttc aacctcccga
     5761 aggaactttc ttctaagctt cagagctttt aagccagccc cgacacccgc caacacccgc
     5821 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgg
     5881 gatgagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     5941 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     6001 aaggaggaac tatatccgga ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     6061 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6121 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6181 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6241 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6301 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6361 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6421 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     6481 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     6541 taaatacatt caaatatgta tccgctcatg aattaattct tagaaaaact catcgagcat
     6601 caaatgaaac tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg
     6661 tttctgtaat gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta
     6721 tcggtctgcg attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa
     6781 aataaggtta tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa
     6841 aagtttatgc atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa
     6901 atcactcgca tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gtcgaaatac
     6961 gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac
     7021 tgccagcgca tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc
     7081 tgttttcccg gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg
     7141 cttgatggtc ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt
     7201 aacatcattg gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt
     7261 cccatacaat cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata
     7321 cccatataaa tcagcatcca tgttggaatt taatcgcggc ctagagcaag acgtttcccg
     7381 ttgaatatgg ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt
     7441 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     7501 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     7561 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     7621 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     7681 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     7741 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     7801 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     7861 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     7921 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     7981 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     8041 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     8101 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     8161 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     8221 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     8281 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     8341 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg
     8401 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg
     8461 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgactccggg
     8521 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa
     8581 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc
     8641 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg
     8701 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg
     8761 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat
     8821 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag
     8881 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag
     8941 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc
     9001 cgcgtttcca gactttacga aacacggaaa cccaagacca ttcatgttgt tgctcaggtc
     9061 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc
     9121 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg
     9181 cgcacccgtg gggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg
     9241 gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac
     9301 aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct
     9361 gccggcacct gtcctacgag ttgcatgata aagaacacag tcataagtgc ggcgacgata
     9421 gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt
     9481 cgagatcccg gtgcctaatg agtgagctaa cttacattaa ttgcgttgcg ctcactgccc
     9541 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     9601 agaggcggtt tgcgtattgg gcgccagggt ggtttttctt ttcaccagtg acacgggcaa
     9661 cagctgattg cccttcaccg cctggccctg agagagttgc agcaagcggt ccacgctggt
     9721 ttgccccagc aggcgaaaat cctgtttgat ggtggttaac ggcgggatat aacatgagct
     9781 gtcctcggta tcgtcgtatc ccactaccga gatatccgca ccaacgcgca gcccggactc
     9841 ggtaatggcg cgcattgcgc ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg
     9901 aacgatgccc tcattcagca tttgcatggt ttgttgaaaa ccggacatgg cactccagtc
     9961 gccttcccgt tccgctatcg gctgaatttg attgcgagtg agatatttat gcc
//
