LOCUS       sequence-205549-       10013 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10013)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10013)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-334
FEATURES             Location/Qualifiers
     source          1..10013
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     584..603
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(623..700)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     906..925
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1009..1028
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1009..1027
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1028..1052
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1106..1123
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             4671..4760
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             4773..4862
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5956..5974)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5970..6017
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6054..6509
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6141..6160)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6351..6372
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(6601..7416)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6601..7416)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7324..7343
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7408..7427
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7538..8126
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8027..8046
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8280..8297
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8312..8451
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8377..8396)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8537..8559
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8553..8744)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9553..10013,1..622))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
       61 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
      121 gcgtaccgtc ctcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
      181 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
      241 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
      301 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
      361 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
      421 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
      481 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
      541 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
      601 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
      661 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc
      721 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
      781 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
      841 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
      901 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
      961 gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaatta atacgactca
     1021 ctatagggga attgtgagcg gataacaatt cccctctaga aataattttg ccttaacttt
     1081 aagaaggaga tataccatgg gcagccacca ccaccaccac cacagcggaa tgcagatctt
     1141 tgttaagact ctcaccggaa agacaatcac cctagaggtg gaaagctccg acaccatcga
     1201 caacgttaag gccaagatcc aggataagga gggcattcct ccggatcagc agaggcttat
     1261 tttcgccggc aagcagctag aggatggccg tacgttggct gattacaata tccagaagga
     1321 atccaccctc cacttggtcc tcaggctccg tggtggttaa gcttgcaatt aactttaaga
     1381 aggagatata caatgcttca caagcgagct agtgaagcta acgataagaa cgataatacc
     1441 atcatcggtt ccgatttagc atcatctaag aaacgccgta tcgatttcac tgaatcttcg
     1501 tctgataagt catcttcgat tttagcttct ggtagtagca ggggttttca cggcgatagc
     1561 gtcgttcagc aaatcgacat ggcttttggt aattcgaacc gtcaggagat tgatgaagat
     1621 ctgcacagtc gccagctcgc cgtctatggt cgtgagacta tgaggcgtct ctttgcttcc
     1681 aatgttctca tctcggggat gcacggtctt ggtgccgaga ttgccaagaa tcttatactt
     1741 gctggtgtga agtctgtgac cttgcatgat gaaagagttg tagagctatg ggacttatca
     1801 agcaactttg ttttctcgga ggatgatgtt ggcaagaata gagcagatgc ttctgttcag
     1861 aagttacagg atcttaacaa tgctgtggtt gtctctagct tgaccaaaag cttaaacaaa
     1921 gaggatcttt ctggcttcca ggttgttgta ttctctgaca taagcatgga aagagcaatc
     1981 gaatttgatg actattgtca cagccatcag cctcctatcg cttttgtcaa ggctgatgtc
     2041 aggggtcttt tcggctctgt cttttgtgat tttgggcctg aatttgcggt tcttgatgtt
     2101 gatggagagg aaccccatac aggcatcatt gcgtctatct ctaatgagaa ccaggcattt
     2161 atttcctgtg tcgacgatga gaggcttgag tttgaagatg gtgacctagt tgttttctca
     2221 gaagttgagg gtatgacaga attgaatgat gggaaaccaa ggaagataaa aagtacacgg
     2281 ccatattcat tcacccttga tgaggacact actaactatg gaacatatgt gaagggtggt
     2341 attgtcactc aagtgaaaca gccaaagtta ctgaatttca agccattgag ggaagcgctt
     2401 aaggatccag gggattttct atttagtgat ttctctaagt ttgatcggcc tccactcctt
     2461 cacttagcat tccaggcact tgatcatttt aaagctgaag ctggcagatt tcctgttgct
     2521 ggctcagaag aggacgctca gaagcttata tcgattgcca cagccatcaa taccgggcag
     2581 ggtgatttaa aggtggagaa tgttgatcag aagcttctaa gacacttttc ctttggagcc
     2641 aaggctgtcc tgaatcctat ggctgcaatg tttggcggta ttgttggaca ggaggttgtt
     2701 aaagcttgct ctggaaaatt tcatcctctc ttccagtttt tctactttga ttcagtggag
     2761 tcactcccct ctgaacctgt ggactcgagt gactttgcac caagaaacag ccggtatgat
     2821 gcccaaatat ctgtatttgg ggccaagttc cagaagaaac ttgaagatgc taaagttttc
     2881 acagtagggt ctggtgctct tggctgtgag ttcttgaaaa atttggctct gatgggggtt
     2941 tcttgtggaa gccaagggaa gctgacagtg acagatgatg atatcattga gaagagtaac
     3001 ctcagtcgtc aatttctgtt ccgtgactgg aacattggac aggctaaatc cacagttgct
     3061 gcttcggctg ctgctgttat aaatcctcgc ttcaacattg aggccctgca aaaccgtgtt
     3121 ggcgcggaga ctgagaatgt atttgacgat gccttctggg agaacttaac tgttgtcgtc
     3181 aatgcactgg ataatgttaa tgcgaggctc tatgttgatt cgaggtgctt gtatttccag
     3241 aagcctctcc ttgagtctgg aactcttggt acaaagtgca acacacagtc ggttatccca
     3301 catctaactg aaaattacgg tgcctcaagg gacccaccag agaaacaggc ccccatgtgt
     3361 acagtgcatt ccttcccgca taacattgat cactgtttaa cttgggctcg ctctgagttt
     3421 gagggtctgc tggagaaaac tcccgctgaa gtgaatgcat atctttctag cccagttgag
     3481 tacactaact caatgatgag tgctggtgat gctcaggcga gggacacatt ggagaggatt
     3541 gttgagtgcc ttgaaaagga gaagtgtgaa accttccaag actgcttaac ctgggcacga
     3601 ctgaggtttg aggattactt tgtgaaccgt gtcaagcaat tgatatacac atttcctgaa
     3661 gatgctgcga ctagtaccgg agctccattc tggtctgctc caaaaagatt ccctcgtcca
     3721 ctccagtact cgtcgtccga cccaagtctc cttaacttta tcacagcgac tgctatttta
     3781 cgagcagaga catttgggat cccgatacct gagtggacta agaacccaaa ggaagcagca
     3841 gaagctgttg acagagtgat agtcccagac tttgagccaa ggcaagatgc aaagattgtg
     3901 actgatgaga aagctaccac tttaaccact gcttcggtgg acgacgctgc agtcatcgac
     3961 gatctcattg ctaagatcga ccagtgtagg cataacttgt cgccagactt caggatgaaa
     4021 cctattcagt tcgaaaagga tgatgataca aactatcaca tggatgtgat agcaggacta
     4081 gccaacatga gggcgagaaa ttacagcatc cctgaagtgg acaagctgaa agcaaagttc
     4141 atagcaggga gaatcatccc agccattgcg acttcaacag ccatggctac tgggctggtc
     4201 tgcctcgagc tgtacaaggt ccttgatgga ggacataaag tggaagctta cagaaacaca
     4261 tttgccaacc tggcacttcc actattctcc atggctgaac cacttccacc aaaggtggtg
     4321 aagcaccgtg acatggcttg gacagtttgg gacagatggg ttctcaaggg aaacccaact
     4381 cttcgtgagg tgttgcagtg gctggaggac aaagggctta gcgcatacag catctcttgt
     4441 ggaagctgtc tactgttcaa cagtatgttc acaaggcaca aggagaggat ggacaagaag
     4501 gttgtggatc tcgctcggga tgttgcgaaa gtcgagttac cgccttatcg caaccatctt
     4561 gatgtagtgg tggcttgtga ggatgaagat gacaatgacg tcgatattcc tctcgtctct
     4621 atctacttca ggtgagctta ctattaactt taagaaggag atataccatg tacccatacg
     4681 atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga ttgtatccat
     4741 atgacgttcc agattacgcc actagagctg cttacccata cgatgttcct gactatgcgg
     4801 gctatcccta tgacgtcccg gactatgcag gattgtatcc atatgacgtt ccagattacg
     4861 caatggccaa cagtaatttg ccgcgaagaa tcatcaagga aactcaacgt ctgctcagtg
     4921 aaccggctcc cggtataagt gcctctccat ctgaggataa tatgcggtat ttcaacgtta
     4981 tgattcttgg tcctacacaa tcaccttatg aaggaggagt tttcaagttg gagctctttt
     5041 tgcctgaaga ataccctatg gcagctccca aggttaggtt tctcacaaag atataccatc
     5101 ctaacattga caagcttgga agaatctgtc ttgatattct caaggacaaa tggagccctg
     5161 cactacaaat acgaacagtg ctcttaagta ttcaagctct tctgagtgca ccaaacccag
     5221 atgatccgct ttctgagaac attgctaagc actggaagag taatgaggca gaagctgtgg
     5281 atacagctaa ggaatggacc cgtctttatg caagcggcgc atgagctttt acttaacttt
     5341 aagaaggaga tatacaatga ctcttggctc aggaggatct agcgttgttg ttccgaggaa
     5401 ttttcggttg ttggaggagc ttgaacgtgg ggagaaaggt attggggatg gaactgtgag
     5461 ctatggaatg gatgatggag atgacatcta tatgcgttct tggactggca ccatcatcgg
     5521 tcctcacaac actgttcatg aaggtagaat ctatcagttg aagctctttt gtgacaaaga
     5581 ttacccggag aaacctccaa ctgtccgatt ccattcacgt gtcaacatgg cttgtgtcaa
     5641 ccacgaaaca ggagtggtgg atcctaagaa atttgggcta cttgcaaact ggcaaagaga
     5701 gtacacaatg gaggatatac tggtacagtt gaagaaggaa atgtctacgt cgcataaccg
     5761 taagcttgtt caacctccgg aaggtacttg cttctaggct tcagagcttt taagccagcc
     5821 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
     5881 ttacagacaa gctgtgaccg ggatgagatc cggctgctaa caaagcccga aaggaagctg
     5941 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
     6001 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
     6061 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     6121 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     6181 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     6241 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     6301 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     6361 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     6421 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     6481 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     6541 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gaattaattc
     6601 ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta ttcatatcag gattatcaat
     6661 accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa aactcaccga ggcagttcca
     6721 taggatggca agatcctggt atcggtctgc gattccgact cgtccaacat caatacaacc
     6781 tattaatttc ccctcgtcaa aaataaggtt atcaagtgag aaatcaccat gagtgacgac
     6841 tgaatccggt gagaatggca aaagtttatg catttctttc cagacttgtt caacaggcca
     6901 gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa ccgttattca ttcgtgattg
     6961 cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga caattacaaa caggaatcga
     7021 atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata ttttcacctg aatcaggata
     7081 ttcttctaat acctggaatg ctgttttccc ggggatcgca gtggtgagta accatgcatc
     7141 atcaggagta cggataaaat gcttgatggt cggaagaggc ataaattccg tcagccagtt
     7201 tagtctgacc atctcatctg taacatcatt ggcaacgcta cctttgccat gtttcagaaa
     7261 caactctggc gcatcgggct tcccatacaa tcgatagatt gtcgcacctg attgcccgac
     7321 attatcgcga gcccatttat acccatataa atcagcatcc atgttggaat ttaatcgcgg
     7381 cctagagcaa gacgtttccc gttgaatatg gctcataaca ccccttgtat tactgtttat
     7441 gtaagcagac agttttattg ttcatgacca aaatccctta acgtgagttt tcgttccact
     7501 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     7561 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     7621 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     7681 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     7741 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     7801 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     7861 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     7921 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     7981 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     8041 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     8101 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     8161 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     8221 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     8281 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc
     8341 tgtgcggtat ttcacaccgc aatggtgcac tctcagtaca atctgctctg atgccgcata
     8401 gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac
     8461 ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga
     8521 caagctgtga ccgactccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa
     8581 cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct
     8641 gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg
     8701 ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa
     8761 gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata
     8821 cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg
     8881 gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat
     8941 acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata
     9001 atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa acccaagacc
     9061 attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg
     9121 cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc
     9181 aacgacagga gcacgatcat gcgcacccgt ggggccgcca tgccggcgat aatggcctgc
     9241 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
     9301 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
     9361 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaacaca
     9421 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
     9481 aaggctctca agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta
     9541 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9601 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct
     9661 tttcaccagt gacacgggca acagctgatt gcccttcacc gcctggccct gagagagttg
     9721 cagcaagcgg tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa
     9781 cggcgggata taacatgagc tgtcctcggt atcgtcgtat cccactaccg agatatccgc
     9841 accaacgcgc agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt
     9901 ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa
     9961 accggacatg gcactccagt cgccttcccg ttccgctatc ggctgaattt gat
//
