LOCUS       sequence-205550-       10049 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10049)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-335
FEATURES             Location/Qualifiers
     source          1..10049
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     81..100
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     184..203
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        184..202
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    203..227
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             281..298
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             3846..3935
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             3948..4037
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(5167..5185)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5181..5228
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5265..5720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5352..5371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5562..5583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(5812..6627)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(5812..6627)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6535..6554
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6619..6638
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      6749..7337
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7238..7257
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7491..7508
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7523..7662
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7588..7607)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7748..7770
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7764..7955)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8764..9846)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9808..9827
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9847..9924)
                     /gene="lacI"
                     /label=lacI promoter
ORIGIN
        1 ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg
       61 cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg
      121 gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga
      181 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
      241 ttttgcctta actttaagaa ggagatatac catgggcagc caccaccacc accaccacag
      301 cggaatgcag atctttgtta agactctcac cggaaagaca atcaccctag aggtggaaag
      361 ctccgacacc atcgacaacg ttaaggccaa gatccaggat aaggagggca ttcctccgga
      421 tcagcagagg cttattttcg ccggcaagca gctagaggat ggccgtacgt tggctgatta
      481 caatatccag aaggaatcca ccctccactt ggtcctcagg ctccgtggtg gttaagcttg
      541 caattaactt taagaaggag atatacaatg cttcacaagc gagctagtga agctaacgat
      601 aagaacgata ataccatcat cggttccgat ttagcatcat ctaagaaacg ccgtatcgat
      661 ttcactgaat cttcgtctga taagtcatct tcgattttag cttctggtag tagcaggggt
      721 tttcacggcg atagcgtcgt tcagcaaatc gacatggctt ttggtaattc gaaccgtcag
      781 gagattgatg aagatctgca cagtcgccag ctcgccgtct atggtcgtga gactatgagg
      841 cgtctctttg cttccaatgt tctcatctcg gggatgcacg gtcttggtgc cgagattgcc
      901 aagaatctta tacttgctgg tgtgaagtct gtgaccttgc atgatgaaag agttgtagag
      961 ctatgggact tatcaagcaa ctttgttttc tcggaggatg atgttggcaa gaatagagca
     1021 gatgcttctg ttcagaagtt acaggatctt aacaatgctg tggttgtctc tagcttgacc
     1081 aaaagcttaa acaaagagga tctttctggc ttccaggttg ttgtattctc tgacataagc
     1141 atggaaagag caatcgaatt tgatgactat tgtcacagcc atcagcctcc tatcgctttt
     1201 gtcaaggctg atgtcagggg tcttttcggc tctgtctttt gtgattttgg gcctgaattt
     1261 gcggttcttg atgttgatgg agaggaaccc catacaggca tcattgcgtc tatctctaat
     1321 gagaaccagg catttatttc ctgtgtcgac gatgagaggc ttgagtttga agatggtgac
     1381 ctagttgttt tctcagaagt tgagggtatg acagaattga atgatgggaa accaaggaag
     1441 ataaaaagta cacggccata ttcattcacc cttgatgagg acactactaa ctatggaaca
     1501 tatgtgaagg gtggtattgt cactcaagtg aaacagccaa agttactgaa tttcaagcca
     1561 ttgagggaag cgcttaagga tccaggggat tttctattta gtgatttctc taagtttgat
     1621 cggcctccac tccttcactt agcattccag gcacttgatc attttaaagc tgaagctggc
     1681 agatttcctg ttgctggctc agaagaggac gctcagaagc ttatatcgat tgccacagcc
     1741 atcaataccg ggcagggtga tttaaaggtg gagaatgttg atcagaagct tctaagacac
     1801 ttttcctttg gagccaaggc tgtcctgaat cctatggctg caatgtttgg cggtattgtt
     1861 ggacaggagg ttgttaaagc ttgctctgga aaatttcatc ctctcttcca gtttttctac
     1921 tttgattcag tggagtcact cccctctgaa cctgtggact cgagtgactt tgcaccaaga
     1981 aacagccggt atgatgccca aatatctgta tttggggcca agttccagaa gaaacttgaa
     2041 gatgctaaag ttttcacagt agggtctggt gctcttggct gtgagttctt gaaaaatttg
     2101 gctctgatgg gggtttcttg tggaagccaa gggaagctga cagtgacaga tgatgatatc
     2161 attgagaaga gtaacctcag tcgtcaattt ctgttccgtg actggaacat tggacaggct
     2221 aaatccacag ttgctgcttc ggctgctgct gttataaatc ctcgcttcaa cattgaggcc
     2281 ctgcaaaacc gtgttggcgc ggagactgag aatgtatttg acgatgcctt ctgggagaac
     2341 ttaactgttg tcgtcaatgc actggataat gttaatgcga ggctctatgt tgattcgagg
     2401 tgcttgtatt tccagaagcc tctccttgag tctggaactc ttggtacaaa gtgcaacaca
     2461 cagtcggtta tcccacatct aactgaaaat tacggtgcct caagggaccc accagagaaa
     2521 caggccccca tgtgtacagt gcattccttc ccgcataaca ttgatcactg tttaacttgg
     2581 gctcgctctg agtttgaggg tctgctggag aaaactcccg ctgaagtgaa tgcatatctt
     2641 tctagcccag ttgagtacac taactcaatg atgagtgctg gtgatgctca ggcgagggac
     2701 acattggaga ggattgttga gtgccttgaa aaggagaagt gtgaaacctt ccaagactgc
     2761 ttaacctggg cacgactgag gtttgaggat tactttgtga accgtgtcaa gcaattgata
     2821 tacacatttc ctgaagatgc tgcgactagt accggagctc cattctggtc tgctccaaaa
     2881 agattccctc gtccactcca gtactcgtcg tccgacccaa gtctccttaa ctttatcaca
     2941 gcgactgcta ttttacgagc agagacattt gggatcccga tacctgagtg gactaagaac
     3001 ccaaaggaag cagcagaagc tgttgacaga gtgatagtcc cagactttga gccaaggcaa
     3061 gatgcaaaga ttgtgactga tgagaaagct accactttaa ccactgcttc ggtggacgac
     3121 gctgcagtca tcgacgatct cattgctaag atcgaccagt gtaggcataa cttgtcgcca
     3181 gacttcagga tgaaacctat tcagttcgaa aaggatgatg atacaaacta tcacatggat
     3241 gtgatagcag gactagccaa catgagggcg agaaattaca gcatccctga agtggacaag
     3301 ctgaaagcaa agttcatagc agggagaatc atcccagcca ttgcgacttc aacagccatg
     3361 gctactgggc tggtctgcct cgagctgtac aaggtccttg atggaggaca taaagtggaa
     3421 gcttacagaa acacatttgc caacctggca cttccactat tctccatggc tgaaccactt
     3481 ccaccaaagg tggtgaagca ccgtgacatg gcttggacag tttgggacag atgggttctc
     3541 aagggaaacc caactcttcg tgaggtgttg cagtggctgg aggacaaagg gcttagcgca
     3601 tacagcatct cttgtggaag ctgtctactg ttcaacagta tgttcacaag gcacaaggag
     3661 aggatggaca agaaggttgt ggatctcgct cgggatgttg cgaaagtcga gttaccgcct
     3721 tatcgcaacc atcttgatgt agtggtggct tgtgaggatg aagatgacaa tgacgtcgat
     3781 attcctctcg tctctatcta cttcaggtga gcttactatt aactttaaga aggagatata
     3841 ccatgtaccc atacgatgtt cctgactatg cgggctatcc ctatgacgtc ccggactatg
     3901 caggattgta tccatatgac gttccagatt acgccactag agctgcttac ccatacgatg
     3961 ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggattg tatccatatg
     4021 acgttccaga ttacgcaatg gccaatagta atcttccccg acgaatcatc aaggaaactc
     4081 aacgtctgct tagtgaacct gctcctggga taagtgcgtc tccatcagag gaaaatatga
     4141 gatatttcaa tgttatgatt cttggtccta ctcaatctcc ttatgaagga ggagttttca
     4201 agttggagct ctttttgcct gaagaatatc ctatggcagc tcccaaggtt aggtttctca
     4261 ccaagattta ccatcctaac atcgacaagc ttggaagaat ctgccttgac atactgaaag
     4321 acaagtggag tcctgcacta caaatacgaa ctgtgctctt gagtatccaa gctcttttaa
     4381 gtgcaccaaa cccagatgat cctctgtctg agaacatagc taagcattgg aagagcaatg
     4441 aggcagaagc tgtggaaaca gctaaggaat ggactcgtct ttacgcaagt ggtgcttaag
     4501 cttttactta actttaagaa ggagatatac aatgagttcg gaggaagcca aggtcgttgt
     4561 tccgaggaac tttagattgt tggaagagct tgaaagaggt gagaaaggta ttggagatgg
     4621 tactgttagc tatggaatgg atgatgctga tgatatttat atgcaatctt ggactggcac
     4681 cattcttggt cctcctaata ctgcatatga agggaaaatc tttcagctga agcttttctg
     4741 tggtaaggag taccctgaaa gtccaccgac tgtgaggttc cagacccgga taaacatggc
     4801 ttgtgttaac ccagaaactg gagtggttga accgagtctc ttccctatgc tcactaactg
     4861 gcggcgagaa tatacaatgg aggacatact ggttaagctg aaaaaagaaa tgatgacttc
     4921 gcataaccgg aagttagctc aacccccgga aggtaacgag gaagctaggg cagatccaaa
     4981 aggaccagct aaatgttgtg tgatgtgagc ttcagagctt ttaagccagc cccgacaccc
     5041 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     5101 agctgtgacc gggatgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg
     5161 ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg
     5221 gttttttgct gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg
     5281 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5341 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5401 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5461 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5521 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5581 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5641 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5701 aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt
     5761 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa
     5821 ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt
     5881 ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc
     5941 aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt
     6001 cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg
     6061 tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg
     6121 ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc
     6181 gagtcgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg
     6241 gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa
     6301 tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt
     6361 acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac
     6421 catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg
     6481 cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg
     6541 agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca
     6601 agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga
     6661 cagttttatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6721 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6781 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6841 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6901 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6961 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     7021 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7081 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7141 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7201 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7261 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7321 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7381 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7441 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7501 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
     7561 tttcacaccg caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     7621 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca
     7681 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg
     7741 accgactccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg
     7801 cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca
     7861 tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg
     7921 gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt
     7981 ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact
     8041 gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg
     8101 cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta
     8161 ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag
     8221 ggcgctgact tccgcgtttc cagactttac gaaacacgga aacccaagac cattcatgtt
     8281 gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt
     8341 gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg
     8401 agcacgatca tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg
     8461 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat
     8521 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg
     8581 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaacac agtcataagt
     8641 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc
     8701 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg
     8761 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
     8821 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag
     8881 tgacacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg
     8941 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat
     9001 ataacatgag ctgtcctcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg
     9061 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag
     9121 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat
     9181 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt
     9241 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc
     9301 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcctcatg
     9361 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac
     9421 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat
     9481 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc
     9541 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat
     9601 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag
     9661 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc
     9721 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac
     9781 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg
     9841 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa
     9901 ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca
     9961 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg
    10021 catgcaagga gatggcgccc aacagtccc
//
