LOCUS       sequence-205553-       10052 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Level 2 construct.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10052)
  AUTHORS   Kowarschik K, Hoehenwarter W, Marillonnet S, Trujillo M
  TITLE     UbiGate: a synthetic biology toolbox to analyse ubiquitination.
  JOURNAL   New Phytol. 2018 Mar;217(4):1749-1763. doi: 10.1111/nph.14900. Epub 
            2017 Nov 30.
  PUBMED    29194629
REFERENCE   2  (bases 1 to 10052)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/nph.14900";
            journalName: "New Phytol"; date: "2018-03"; volume: "217"; issue: 
            "4"; pages: "1749-1763"
COMMENT     Sequence Label: pUG-336
FEATURES             Location/Qualifiers
     source          1..10052
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     946..965
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(985..1062)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     1268..1287
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     1371..1390
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1371..1389
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1390..1414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             1468..1485
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5033..5122
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     CDS             5135..5224
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGLYPYDVPDYA"
     primer_bind     complement(6357..6375)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      6371..6418
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      6455..6910
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6542..6561)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6752..6773
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(7002..7817)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7002..7817)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     7725..7744
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     7809..7828
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      7939..8527
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8428..8447
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8681..8698
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8713..8852
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8778..8797)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8938..8960
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(8954..9145)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(join(9954..10052,1..984))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 ggtttttctt ttcaccagtg acacgggcaa cagctgattg cccttcaccg cctggccctg
       61 agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat cctgtttgat
      121 ggtggttaac ggcgggatat aacatgagct gtcctcggta tcgtcgtatc ccactaccga
      181 gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc ccagcgccat
      241 ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca tttgcatggt
      301 ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg gctgaatttg
      361 attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga cagaacttaa
      421 tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct ccacgcccag
      481 tcgcgtaccg tcctcatggg agaaaataat actgttgatg ggtgtctggt cagagacatc
      541 aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat cctggtcatc
      601 cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt gcaccgccgc
      661 tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg cacccagttg
      721 atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg ccagactgga
      781 ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca cgcggttggg
      841 aatgtaattc agctccgcca tcgccgcttc cactttttcc cgcgttttcg cagaaacgtg
      901 gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat actctgcgac
      961 atcgtataac gttactggtt tcacattcac caccctgaat tgactctctt ccgggcgcta
     1021 tcatgccata ccgcgaaagg ttttgcgcca ttcgatggtg tccgggatct cgacgctctc
     1081 ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg
     1141 ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc
     1201 ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt
     1261 ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc
     1321 cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat taatacgact
     1381 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgccttaact
     1441 ttaagaagga gatataccat gggcagccac caccaccacc accacagcgg aatgcagatc
     1501 tttgttaaga ctctcaccgg aaagacaatc accctagagg tggaaagctc cgacaccatc
     1561 gacaacgtta aggccaagat ccaggataag gagggcattc ctccggatca gcagaggctt
     1621 attttcgccg gcaagcagct agaggatggc cgtacgttgg ctgattacaa tatccagaag
     1681 gaatccaccc tccacttggt cctcaggctc cgtggtggtt aagcttgcaa ttaactttaa
     1741 gaaggagata tacaatgctt cacaagcgag ctagtgaagc taacgataag aacgataata
     1801 ccatcatcgg ttccgattta gcatcatcta agaaacgccg tatcgatttc actgaatctt
     1861 cgtctgataa gtcatcttcg attttagctt ctggtagtag caggggtttt cacggcgata
     1921 gcgtcgttca gcaaatcgac atggcttttg gtaattcgaa ccgtcaggag attgatgaag
     1981 atctgcacag tcgccagctc gccgtctatg gtcgtgagac tatgaggcgt ctctttgctt
     2041 ccaatgttct catctcgggg atgcacggtc ttggtgccga gattgccaag aatcttatac
     2101 ttgctggtgt gaagtctgtg accttgcatg atgaaagagt tgtagagcta tgggacttat
     2161 caagcaactt tgttttctcg gaggatgatg ttggcaagaa tagagcagat gcttctgttc
     2221 agaagttaca ggatcttaac aatgctgtgg ttgtctctag cttgaccaaa agcttaaaca
     2281 aagaggatct ttctggcttc caggttgttg tattctctga cataagcatg gaaagagcaa
     2341 tcgaatttga tgactattgt cacagccatc agcctcctat cgcttttgtc aaggctgatg
     2401 tcaggggtct tttcggctct gtcttttgtg attttgggcc tgaatttgcg gttcttgatg
     2461 ttgatggaga ggaaccccat acaggcatca ttgcgtctat ctctaatgag aaccaggcat
     2521 ttatttcctg tgtcgacgat gagaggcttg agtttgaaga tggtgaccta gttgttttct
     2581 cagaagttga gggtatgaca gaattgaatg atgggaaacc aaggaagata aaaagtacac
     2641 ggccatattc attcaccctt gatgaggaca ctactaacta tggaacatat gtgaagggtg
     2701 gtattgtcac tcaagtgaaa cagccaaagt tactgaattt caagccattg agggaagcgc
     2761 ttaaggatcc aggggatttt ctatttagtg atttctctaa gtttgatcgg cctccactcc
     2821 ttcacttagc attccaggca cttgatcatt ttaaagctga agctggcaga tttcctgttg
     2881 ctggctcaga agaggacgct cagaagctta tatcgattgc cacagccatc aataccgggc
     2941 agggtgattt aaaggtggag aatgttgatc agaagcttct aagacacttt tcctttggag
     3001 ccaaggctgt cctgaatcct atggctgcaa tgtttggcgg tattgttgga caggaggttg
     3061 ttaaagcttg ctctggaaaa tttcatcctc tcttccagtt tttctacttt gattcagtgg
     3121 agtcactccc ctctgaacct gtggactcga gtgactttgc accaagaaac agccggtatg
     3181 atgcccaaat atctgtattt ggggccaagt tccagaagaa acttgaagat gctaaagttt
     3241 tcacagtagg gtctggtgct cttggctgtg agttcttgaa aaatttggct ctgatggggg
     3301 tttcttgtgg aagccaaggg aagctgacag tgacagatga tgatatcatt gagaagagta
     3361 acctcagtcg tcaatttctg ttccgtgact ggaacattgg acaggctaaa tccacagttg
     3421 ctgcttcggc tgctgctgtt ataaatcctc gcttcaacat tgaggccctg caaaaccgtg
     3481 ttggcgcgga gactgagaat gtatttgacg atgccttctg ggagaactta actgttgtcg
     3541 tcaatgcact ggataatgtt aatgcgaggc tctatgttga ttcgaggtgc ttgtatttcc
     3601 agaagcctct ccttgagtct ggaactcttg gtacaaagtg caacacacag tcggttatcc
     3661 cacatctaac tgaaaattac ggtgcctcaa gggacccacc agagaaacag gcccccatgt
     3721 gtacagtgca ttccttcccg cataacattg atcactgttt aacttgggct cgctctgagt
     3781 ttgagggtct gctggagaaa actcccgctg aagtgaatgc atatctttct agcccagttg
     3841 agtacactaa ctcaatgatg agtgctggtg atgctcaggc gagggacaca ttggagagga
     3901 ttgttgagtg ccttgaaaag gagaagtgtg aaaccttcca agactgctta acctgggcac
     3961 gactgaggtt tgaggattac tttgtgaacc gtgtcaagca attgatatac acatttcctg
     4021 aagatgctgc gactagtacc ggagctccat tctggtctgc tccaaaaaga ttccctcgtc
     4081 cactccagta ctcgtcgtcc gacccaagtc tccttaactt tatcacagcg actgctattt
     4141 tacgagcaga gacatttggg atcccgatac ctgagtggac taagaaccca aaggaagcag
     4201 cagaagctgt tgacagagtg atagtcccag actttgagcc aaggcaagat gcaaagattg
     4261 tgactgatga gaaagctacc actttaacca ctgcttcggt ggacgacgct gcagtcatcg
     4321 acgatctcat tgctaagatc gaccagtgta ggcataactt gtcgccagac ttcaggatga
     4381 aacctattca gttcgaaaag gatgatgata caaactatca catggatgtg atagcaggac
     4441 tagccaacat gagggcgaga aattacagca tccctgaagt ggacaagctg aaagcaaagt
     4501 tcatagcagg gagaatcatc ccagccattg cgacttcaac agccatggct actgggctgg
     4561 tctgcctcga gctgtacaag gtccttgatg gaggacataa agtggaagct tacagaaaca
     4621 catttgccaa cctggcactt ccactattct ccatggctga accacttcca ccaaaggtgg
     4681 tgaagcaccg tgacatggct tggacagttt gggacagatg ggttctcaag ggaaacccaa
     4741 ctcttcgtga ggtgttgcag tggctggagg acaaagggct tagcgcatac agcatctctt
     4801 gtggaagctg tctactgttc aacagtatgt tcacaaggca caaggagagg atggacaaga
     4861 aggttgtgga tctcgctcgg gatgttgcga aagtcgagtt accgccttat cgcaaccatc
     4921 ttgatgtagt ggtggcttgt gaggatgaag atgacaatga cgtcgatatt cctctcgtct
     4981 ctatctactt caggtgagct tactattaac tttaagaagg agatatacca tgtacccata
     5041 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gattgtatcc
     5101 atatgacgtt ccagattacg ccactagagc tgcttaccca tacgatgttc ctgactatgc
     5161 gggctatccc tatgacgtcc cggactatgc aggattgtat ccatatgacg ttccagatta
     5221 cgcaatggcc aatagtaatc ttccccgacg aatcatcaag gaaactcaac gtctgcttag
     5281 tgaacctgct cctgggataa gtgcgtctcc atcagaggaa aatatgagat atttcaatgt
     5341 tatgattctt ggtcctactc aatctcctta tgaaggagga gttttcaagt tggagctctt
     5401 tttgcctgaa gaatatccta tggcagctcc caaggttagg tttctcacca agatttacca
     5461 tcctaacatc gacaagcttg gaagaatctg ccttgacata ctgaaagaca agtggagtcc
     5521 tgcactacaa atacgaactg tgctcttgag tatccaagct cttttaagtg caccaaaccc
     5581 agatgatcct ctgtctgaga acatagctaa gcattggaag agcaatgagg cagaagctgt
     5641 ggaaacagct aaggaatgga ctcgtcttta cgcaagtggt gcttaagctt ttacttaact
     5701 ttaagaagga gatatacaat gggttcggaa gaagagaagg tcgttgttcc cagaaacttt
     5761 agattattgg aagagctaga aagaggtgag aaaggaattg gagatgggac agtgagttat
     5821 ggaatggatg atgctgatga tattttgatg caatcttgga ctggtaccat tcttggtcct
     5881 cataatactg cgtatgaagg gaaaatcttt cagctgaagc tattctgtgg aaaggattat
     5941 ccggaaagtc cacctactgt gaggtttcag agtcgtataa acatggcttg tgtcaatcct
     6001 gagaatggag tggttgatcc gagtcacttc cctatgcttt ccaactggag aagagaattc
     6061 acaatggagg atctactgat tcagcttaaa aaagaaatga tgtcatcgca gaaccgcaag
     6121 ttagctcaac ccttggaagg taatgaggaa ggcaggacag acccaaaggg actagtggtg
     6181 aaatgttgcg tgatgtgagc ttcagagctt ttaagccagc cccgacaccc gccaacaccc
     6241 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     6301 gggatgagat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc
     6361 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
     6421 gaaaggagga actatatccg gattggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6481 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6541 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6601 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6661 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6721 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6781 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6841 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6901 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6961 tctaaataca ttcaaatatg tatccgctca tgaattaatt cttagaaaaa ctcatcgagc
     7021 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     7081 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     7141 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     7201 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     7261 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     7321 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagtcgaaat
     7381 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     7441 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     7501 gctgttttcc cggggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     7561 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     7621 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     7681 ttcccataca atcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     7741 tacccatata aatcagcatc catgttggaa tttaatcgcg gcctagagca agacgtttcc
     7801 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     7861 gttcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7921 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     7981 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8041 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     8101 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8161 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8221 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8281 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8341 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8401 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8461 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8521 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8581 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8641 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8701 agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg
     8761 caatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc
     8821 cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg
     8881 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgactccg
     8941 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt
     9001 aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca
     9061 gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa
     9121 gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg
     9181 gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac
     9241 atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc
     9301 agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac
     9361 agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact
     9421 tccgcgtttc cagactttac gaaacacgga aacccaagac cattcatgtt gttgctcagg
     9481 tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct
     9541 gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca
     9601 tgcgcacccg tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg
     9661 tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg
     9721 acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg
     9781 ctgccggcac ctgtcctacg agttgcatga taaagaacac agtcataagt gcggcgacga
     9841 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
     9901 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
     9961 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    10021 ggagaggcgg tttgcgtatt gggcgccagg gt
//
